Online citations, reference lists, and bibliographies.
← Back to Search

Calcium And Lipoprotein Lipase Synergistically Enhance The Binding And Uptake Of Native And Oxidized LDL In Mouse Peritoneal Macrophages.

X. Wang, J. Greilberger, G. Juergens
Published 2000 · Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The influence of Ca(2+) and Mg(2+), together with lipoprotein lipase (LPL), on the binding and uptake of Eu(3+)-labeled native and oxidized low density lipoprotein (LDL) to mouse peritoneal macrophages (MPM), and on the deposition of esterified cholesterol in these macrophages, were studied. We found that both LPL and Ca(2+) (but not Mg(2+)) increased the binding and uptake of native and mildly or moderately oxidized LDL, and the subsequent deposition of cholesterol esters in MPM. When added together, LPL and Ca(2+) synergistically increased the binding and uptake of native and oxidized LDL, and the deposition of esterified cholesterol derived from native and mildly or moderately oxidized LDL, in MPM. Since both calcium and LPL are found in the atherosclerotic lesions, our results suggest that Ca(2+) and LPL may synergistically promote foam cell formation and atherogenesis. Furthermore, future research in the metabolism of lipoproteins should take into account the calcium levels in the experimental conditions.
This paper references
10.1016/0003-9861(88)90437-7
Binding of calcium to glycosaminoglycans: an equilibrium dialysis study.
G. Hunter (1988)
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
10.1161/01.ATV.17.11.2721
In vitro interactions of oxidatively modified LDL with type I, II, III, IV, and V collagen, laminin, fibronectin, and poly-D-lysine.
J. Greilberger (1997)
10.1172/JCI115747
Lipoprotein lipase is synthesized by macrophage-derived foam cells in human coronary atherosclerotic plaques.
K. O'brien (1992)
10.1006/ABIO.1998.3023
Time-resolved fluorometric assay for measuring cell binding and association of native and oxidized low-density lipoproteins to macrophages.
X. Wang (1999)
10.1042/BJ3140563
Lipoprotein lipase stimulates the binding and uptake of moderately oxidized low-density lipoprotein by J774 macrophages.
W. L. Hendriks (1996)
10.1016/0891-5849(92)90181-F
The role of lipid peroxidation and antioxidants in oxidative modification of LDL.
H. Esterbauer (1992)
10.1016/0009-3084(87)90070-3
Modification of human serum low density lipoprotein by oxidation--characterization and pathophysiological implications.
G. Juergens (1987)
10.1042/BJ3430347
Endogenously produced lipoprotein lipase enhances the binding and cell association of native, mildly oxidized and moderately oxidized low-density lipoprotein in mouse peritoneal macrophages.
Xiaosong Wang (1999)
10.1146/ANNUREV.BI.46.070177.004341
The low-density lipoprotein pathway and its relation to atherosclerosis.
J. Goldstein (1977)
10.1006/BBRC.1993.1888
Lipoprotein lipase facilitates very low density lipoprotein binding to the subendothelial cell matrix.
U. Saxena (1993)
10.1016/0006-291X(92)91664-C
Heparan sulphate proteoglycans are involved in the lipoprotein lipase-mediated enhancement of the cellular binding of very low density and low density lipoproteins.
M. Mulder (1992)
10.1016/0006-291X(86)91237-4
Apolipoprotein B is a calcium binding protein.
N. Dashti (1986)
Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.
W. Gamble (1978)
Calcium induces a conformational change in the ligand binding domain of the low density lipoprotein receptor.
K. A. Dirlam-Schatz (1998)
10.1097/00041433-199404000-00008
Structure, function and role of lipoprotein lipase in lipoprotein metabolism.
S. Santamarina-Fojo (1994)
10.1016/0021-9150(87)90263-2
The effect of ethane-1-hydroxy-1,1-diphosphonate (EHDP) on necrosis of atherosclerotic lesions.
A. S. Daoud (1987)
Release of endothelial cell lipoprotein lipase by plasma lipoproteins and free fatty acids.
U. Saxena (1989)
10.1021/BI00246A013
Extensive segregation of acidic phospholipids in membranes induced by protein kinase C and related proteins.
M. Bazzi (1991)
10.1042/BJ3300765
Not the mature 56 kDa lipoprotein lipase protein but a 37 kDa protein co-purifying with the lipase mediates the binding of low density lipoproteins to J774 macrophages.
W. L. Hendriks (1998)
Cellular differences in lipoprotein lipase-mediated uptake of low density lipoproteins.
J. Obunike (1994)
10.1172/JCI116018
Lipoprotein lipase-mediated uptake and degradation of low density lipoproteins by fibroblasts and macrophages.
S. Rumsey (1992)
10.1002/jlb.47.1.79
Control of Lipoprotein Lipase Secretion by Macrophages: Effect of Macrophage Differentiation Agents
R. Goldman (1990)
10.1073/PNAS.88.12.5252
Expression of monocyte chemoattractant protein 1 in macrophage-rich areas of human and rabbit atherosclerotic lesions.
S. Ylä-Herttuala (1991)
10.1073/PNAS.88.19.8342
Lipoprotein lipase enhances the binding of chylomicrons to low density lipoprotein receptor-related protein.
U. Beisiegel (1991)
10.1097/00041433-199710000-00003
Cell-surface heparan sulfate proteoglycans: dynamic molecules mediating ligand catabolism
Kevin Jon Williams (1997)
10.1074/jbc.272.34.20963
Low Density Lipoprotein Oxidation and Its Pathobiological Significance*
D. Steinberg (1997)
10.1006/ABIO.1993.1531
Biotinylation of lipoprotein lipase and hepatic triglyceride lipase: application in the assessment of cell binding sites.
P. Sivaram (1993)
A spectrophotometric assay for lipid peroxides in serum lipoproteins using a commercially available reagent.
M. el-Saadani (1989)



This paper is referenced by
10.1016/S0008-6363(02)00405-4
The pivotal role of lipoprotein lipase in atherosclerosis.
J. R. Mead (2002)
ФАРМАКОЛОГИЧЕСКАЯ КОРРЕКЦИЯ ГИПЕРХОЛЕСТЕРИНЕМИИ И АТЕРОГЕННЫХ ПОВРЕЖДЕНИЙ: ВОЗМОЖНОСТИ ВОССТАНОВЛЕНИЯ СТРУКТУРЫ И МЕТАБОЛИЗМА СЕРДЕЧНОЙ МЫШЦЫ
E. I. Yuzhik (2013)
Role of Intracellular Oxidant Release in Oxidised Low Lipoprotein - Induced U937 Cell Death
Alpha Yan Chen (2012)
10.1016/j.nutres.2009.05.001
Inadequate dietary magnesium intake increases atherosclerotic plaque development in rabbits.
Jennifer L. King (2009)
10.1016/J.CATCOM.2012.04.010
Biomimetic oxidation of guggulsterone with hydrogen peroxide catalyzed by iron(III)porphyrins in ionic liquid
Anchal Singhal (2012)
Lipoprotein lipase-unstable on purpose?
L. Zhang (2007)
10.1016/j.ejphar.2010.11.017
Ginsenoside-Rd, a purified component from panax notoginseng saponins, prevents atherosclerosis in apoE knockout mice.
J. Li (2011)
10.1007/s00109-002-0384-9
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
10.1074/jbc.M507252200
Calcium Triggers Folding of Lipoprotein Lipase into Active Dimers*
L. Zhang (2005)
10.1016/J.ATHEROSCLEROSIS.2006.01.007
LPL-mediated lipolysis of VLDL induces an upregulation of AU-rich mRNAs and an activation of HuR in endothelial cells.
M. M. Tschernatsch (2006)
10.1002/path.935
Oxidative modifications of LDL increase its binding to extracellular matrix from human aortic intima: influence of lesion development, lipoprotein lipase and calcium
X. Wang (2001)
10.1074/JBC.M011090200
Binding of Low Density Lipoproteins to Lipoprotein Lipase Is Dependent on Lipids but Not on Apolipoprotein B*
J. Borén (2001)
10.1016/J.ATHEROSCLEROSIS.2003.10.008
The hypolipidemic natural product Commiphora mukul and its component guggulsterone inhibit oxidative modification of LDL.
X. Wang (2004)
10.1172/JCI16484
Hepatic lipase expression in macrophages contributes to atherosclerosis in apoE-deficient and LCAT-transgenic mice.
Z. Nong (2003)
10.1097/00041433-200210000-00002
Lipoprotein lipase: the regulation of tissue specific expression and its role in lipid and energy metabolism
Karina Preiss-Landl (2002)
Semantic Scholar Logo Some data provided by SemanticScholar