Referencing for people who value simplicity, privacy, and speed.Get Citationsy
← Back to Search
Electron Microscopy Of Scallop Myosin. Location Of Regulatory Light Chains.
P. Flicker, T. Wallimann, P. Vibert
Published 1983 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
The heads of the Ca2+-sensitive myosin molecules from scallop muscle, contrasted for electron microscopy by rotary shadowing, display two appearances depending on the presence or absence of the regulatory light chains. The heads of intact myosin appear "pear-shaped" as described for vertebrate myosin (Elliott & Offer, 1978): they are widest at the end remote from the tail and taper to a narrower neck near their junction with the tail. In contrast, myosin heads that lack the regulatory light chains appear more globular. The neck region is no longer visible: the rounded heads appear directly attached to the tail or there is an apparent gap between the head and the tail. Two preparations of myosin subfragment-1 that differ in light chain content show a similar difference in appearance. Fab fragments of antibodies specific for the light chains bind to the myosin heads and can also be visualized in the electron microscope using rotary shadowing. Both Fab fragments specific for the regulatory light chains and Fab fragments specific for the essential light chains bind preferentially to intact scallop myosin in the narrow region of the myosin head near its junction with the tail.
This paper references
Regulatory and essential light-chain interactions in scallop myosin. I. Protection of essential light-chain thiol groups by regulatory light-chains.
P. Hardwicke (1982)
Regulation in molluscan muscles.
J. Kendrick-Jones (1970)
Physical characterization of myosin light chains.
W. Stafford (1978)
Cooperativity in scallop myosin.
Peter D. Chantler (1981)
Light-chain phosphorylation controls the conformation of vertebrate non-muscle and smooth muscle myosin molecules
R. Craig (1983)
Electron microscopy of thin filaments decorated with a Ca2+-regulated myosin.
R. Craig (1980)
Regulatory light chains in myosins.
J. Kendrick-Jones (1976)
Light-chain movement and regulation in scallop myosin
P. Hardwicke (1983)
Regulatory and essential light-chain interactions in scallop myosin. II. Photochemical cross-linking of regulatory and essential light-chains by heterobifunctional reagents.
T. Wallimann (1982)
Light chains of carp and pike skeletal muscle myosins. Isolation and characterization of the most anodic light chain on alkaline pH electrophoresis
B. Focant (1976)
Changes in crossbridge attachment in a myosin-regulated muscle
P. Vibert (1978)
Regulatory properties of single-headed fragments of scallop myosin.
W. Stafford (1979)
Modulator protein as a component of the myosin light chain kinase from chicken gizzard.
R. Dąbrowska (1978)
The molecular structure of human erythrocyte spectrin. Biophysical and electron microscopic studies.
D. Shotton (1979)
Regulatory light-chains and scallop myosin. Full dissociation, reversibility and co-operative effects.
Peter D. Chantler (1980)
SDS microslab linear gradient polyacrylamide gel electrophoresis.
P. Matsudaira (1978)
Rotary shadowing of extended molecules dried from glycerol.
J. Tyler (1980)
Periodic charge distributions in the myosin rod amino acid sequence match cross-bridge spacings in muscle
A. McLachlan (1982)
Tropomyosin Paracrystals Formed by Divalent Cations
C. Cohen (1966)
An immunological approach to myosin light-chain function in thick filament linked regulation. 2. Effects of anti-scallop myosin light-chain antibodies. Possible regulatory role for the essential light chain.
T. Wallimann (1981)
Topography of the myosin molecule as visualized by an improved negative staining method.
K. Takahashi (1978)
The light chains of scallop myosin as regulatory subunits.
A. G. Szent-Györgyi (1973)
Three-dimensional reconstruction of thin filaments decorated with a Ca2+-regulated myosin.
P. Vibert (1982)
Control of tension development in scallop muscle fibres with foreign regulatory light chains
R. M. Simmons (1980)
Regulation of muscular contraction. Distribution of actin control and myosin control in the animal kingdom
W. Lehman (1975)
Reversible loss of calcium control of tension in scallop striated muscle associated with the removal of regulatory light chains
R. M. Simmons (1978)
Substructure of the myosin molecule. I. Subfragments of myosin by enzymic degradation.
S. Lowey (1969)
Shape and flexibility of the myosin molecule.
A. Elliott (1978)
An immunological approach to myosin light-chain function in thick filament linked regulation. 1. Characterization, specificity, and cross-reactivity of anti-scallop myosin heavy- and light-chain antibodies by competitive, solid-phase radioimmunoassay.
T. Wallimann (1981)
Purification and characterization of smooth muscle myosin light chain kinase.
R. Adelstein (1981)
This paper is referenced by
Structural relationships of actin, myosin, and tropomyosin revealed by cryo-electron microscopy
R. Milligan (1987)
Structure of myosin decorated actin filaments and natural thin filaments
J. Seymour (2004)
The substructure of isolated and in situ outer dynein arms of sea urchin sperm flagella
W. Sale (1985)
The length of myosin subfragment-one
R. Mendelson (1985)
Interaction between the heavy and the regulatory light chains in smooth muscle myosin subfragment 1.
H. Onishi (1992)
Tryptic digestion of scallop S1: evidence for a complex between the two light-chains and a heavy-chain peptide
E. Szentkiralyi (2004)
Orientation of spin-labeled light chain-2 exchanged onto myosin cross-bridges in glycerinated muscle fibers.
B. Hambly (1991)
A point mutation in the regulatory light chain reduces the step size of skeletal muscle myosin.
J. J. Sherwood (2004)
Calcium-induced Mechanical Change in the Neck Domain Alters the Activity of Plant Myosin XI*
Motoki Tominaga (2012)
Structure and structural change of the myosin head.
M. Tokunaga (1991)
Structural Changes that upon Activation Occur in Scallop Myosin Filaments
P. Vibert (2002)
Negative staining of myosin molecules.
M. Walker (1985)
Calcium-induced quenching of intrinsic fluorescence in brain myosin V is linked to dissociation of calmodulin light chains.
L. C. Cameron (1998)
Biological cryo atomic force microscopy: a brief review.
Z. Shao (1996)
Domain structure of the myosin head in correlation-averaged images of shadowed molecules
P. Vibert (2005)
Chapter 2 – Myosin Light Chains
K. Bárány (1996)
Three-dimensional structure of myosin subfragment-1 from electron microscopy of sectioned crystals
D. Winkelmann (1991)
Monoclonal antibodies localize changes on myosin heavy chain isozymes during avian myogenesis
D. Winkelmann (1983)
Studies of the morphology and structure of the plasma lipid transfer particle from the tobacco hornworm, Manduca sexta.
R. Ryan (1990)
Role of gizzard myosin light chains in calcium binding
H. Kwon (2005)
Myosin binding to actin. Structural analysis using myosin fragments.
L. Castellani (1987)
A model for fibrinogen: domains and sequence.
J. Weisel (1985)
Packing analysis of crystalline myosin subfragment-1. Implications for the size and shape of the myosin head.
D. Winkelmann (1985)
Electron microscopy of cross-linked scallop myosin.
P. Vibert (1985)
Monoclonal antibodies detect and stabilize conformational states of smooth muscle myosin
K. Trybus (1989)
Direct determination of myosin filament symmetry in scallop striated adductor muscle by rapid freezing and freeze substitution.
R. Craig (1991)
Electron microscopy of cardiac myosin: Its shape and properties as determined by the regulatory light chain
S. Margossian (2005)
Phosphorylation-dependent Structural Changes in the Regulatory Light Chain Domain of Smooth Muscle Heavy Meromyosin*
X. Wu (1999)
Subunit structure of junctional feet in triads of skeletal muscle: a freeze-drying, rotary-shadowing study
D. G. Ferguson (1984)
Amino acid sequence of myosin essential light chain from the scallop Aquipecten irradians.
J. Collins (1986)
Molecular Biology of Muscle Development
J. Karn (1987)
Shape of the myosin head in the rigor complex. Three-dimensional image reconstruction of the actin-tropomyosin-heavy meromyosin complex.
H. Kajiyama (1988)See more