Online citations, reference lists, and bibliographies.
← Back to Search

Polysaccharide Degradation By Fenton Reaction--or Peroxidase-generated Hydroxyl Radicals In Isolated Plant Cell Walls.

C. Schweikert, A. Liszkay, P. Schopfer
Published 2002 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The formation of hydroxyl radicals (OH*) by peroxidase was confirmed by EPR spectroscopy using ethanol/alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone as a spin-trapping system specific of OH*. The effect of OH*, generated either non-enzymatically with the Fenton reaction (H(2)O(2) + Fe(2+)) or with horseradish peroxidase in the presence of O(2) and NADH, on cell walls isolated from maize (Zea mays) coleoptiles or soybean (Glycine max) hypocotyls was investigated. OH* produced by these reactions attack polysaccharides in the wall, demonstrated by the release of a heterogeneous mixture of polymeric breakdown products into the incubation medium. The peroxidase-catalyzed degradation of cell-wall polysaccharides can be inhibited by KCN and superoxide radical (O(2)*) or OH* scavengers. These data support the hypothesis that OH*, produced by cell-wall peroxidases in vivo, act as wall-loosening agents in plant extension growth.
This paper references
10.1104/PP.95.4.1012
Acid- and Enzyme-Mediated Solubilization of Cell-Wall β-1.3,β-1.4-d-Glucan in Maize Coleoptiles : Implications for Auxin-Mediated Growth
M. Hohl (1991)
10.1016/0003-9861(89)90265-8
Characterization of a superoxide dismutase mimic prepared from desferrioxamine and MnO2.
W. Beyer (1989)
10.1104/PP.117.2.491
Use of a new tetrazolium-based assay to study the production of superoxide radicals by tobacco cell cultures challenged with avirulent zoospores of phytophthora parasitica var nicotianae
Able (1998)
10.1006/PMPP.1996.0076
Evidence for a primary role of active oxygen species in induction of host cell death during infection of bean leaves withBotrytis cinerea
A. V. Tiedemann (1997)
10.1046/J.1432-1327.1999.00199.X
Hydroxyl-radical production in physiological reactions. A novel function of peroxidase.
S. X. Chen (1999)
10.1016/S0891-5849(87)80007-2
Effects of paraquat on the green alga Dunaliella salina: protection by the mimic of superoxide dismutase, Desferal-Mn(IV).
H. Rabinowitch (1987)
10.1104/PP.65.5.768
beta-d-Glucan Hydrolase Activity in Zea Coleoptile Cell Walls.
D. Huber (1980)
10.1016/S0076-6879(84)05026-6
[23] Spin trapping of superoxide and hydroxyl radicals
G. Rosen (1984)
10.1042/BJ3320507
Oxidative scission of plant cell wall polysaccharides by ascorbate-induced hydroxyl radicals.
S. Fry (1998)
10.1021/AC60111A017
Colorimetric Method for Determination of Sugars and Related Substances
M. DuBois (1956)
10.1021/JA01594A086
Methods of Enzymology.
H. Tauber (1956)
10.1007/s004250000376
NADH-stimulated, cyanide-resistant superoxide production in maize coleoptiles analyzed with a tetrazolium-based assay
G. Frahry (2001)
10.1016/S0031-9422(99)00586-5
Scission of polysaccharides by peroxidase-generated hydroxyl radicals.
C. Schweikert (2000)
10.1016/s0021-9258(18)42443-x
Spin trapping evidence for myeloperoxidase-dependent hydroxyl radical formation by human neutrophils and monocytes.
Carroll L. Ramos (1992)
10.1007/s00425-001-0699-8
Evidence that hydroxyl radicals mediate auxin-induced extension growth
P. Schopfer (2001)
10.1111/J.1365-313X.1993.TB00007.X
Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth.
N. Carpita (1993)
10.1016/S0926-6593(65)80154-0
Reaction of peroxidase with reduced nicotinamide-adenine dinucleotide and reduced nicotinamide-adenine dinucleotide phosphate.
K. Yokota (1965)
10.1104/PP.125.4.1591
Release of reactive oxygen intermediates (superoxide radicals, hydrogen peroxide, and hydroxyl radicals) and peroxidase in germinating radish seeds controlled by light, gibberellin, and abscisic acid.
P. Schopfer (2001)
10.1046/J.1365-313X.2001.01187.X
Hydroxyl radical-induced cell-wall loosening in vitro and in vivo: implications for the control of elongation growth.
P. Schopfer (2001)



This paper is referenced by
10.4172/2376-0354.1000110
The Role - Activity and/or Processing - Of (Re) Active Oxygen Species inDesiccation Sensitivity and/or Tolerance, Development, Dormancy and/orGermination in Seeds
Tobias M. Ntuli (2014)
10.1016/J.SCIENTA.2013.05.048
Modification of cell wall polysaccharide during ripening of Chinese bayberry fruit
Sun Xuke (2013)
Solubilisation of tomato fruit pectins by ascorbate: a possible non-enzymic mechanism of fruit softening
Daniel Rutherford Building (2003)
10.4236/AJPS.2011.21005
Participation of Chitin-Binding Peroxidase Isoforms in the Wilt Pathogenesis of Cotton
E. Pshenichnov (2011)
10.1093/jxb/err359
Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast.
J. D. Dragišić Maksimović (2012)
10.1093/jxb/eru167
Involvement of reactive oxygen species in endosperm cap weakening and embryo elongation growth during lettuce seed germination
Y. Zhang (2014)
10.1101/2020.08.11.239541
Proteomics reveals synergy in biomass conversion between fungal enzymes and inorganic Fenton chemistry in leaf-cutting ant colonies
M. Schiøtt (2020)
10.1007/s11738-017-2596-1
Effects of calcium and EGTA on thiol homeostasis and defense-related enzymes in Cd-exposed chickpea roots
Lamia Sakouhi (2017)
10.7554/eLife.61816
Proteomics reveals synergy between biomass degrading enzymes and inorganic Fenton chemistry in leaf-cutting ant colonies
M. Schiøtt (2021)
10.1007/s00425-003-1061-0
Solubilisation of tomato fruit pectins by ascorbate: a possible non-enzymic mechanism of fruit softening
J. Dumville (2003)
10.1016/J.CARBPOL.2004.09.008
Storage stability of laccase induced arabinoxylan gels
E. Carvajal-Millán (2005)
10.1016/J.ENVEXPBOT.2007.08.004
Sub-cellular location of H2O2, peroxidases and pectin epitopes in control and hyperhydric shoots of carnation
N. Fernández-García (2008)
10.3732/ajb.93.10.1415
Biomechanics of plant growth.
P. Schopfer (2006)
10.3929/ETHZ-A-009785350
Hydroxyl radical mediated degradation of cereal β-glucan
Audrey M. Faure (2013)
10.1002/9781119312994.APR0074
Expansion of the Plant Cell Wall
D. Cosgrove (2018)
10.1093/jxb/err336
Ascorbic acid and reactive oxygen species are involved in the inhibition of seed germination by abscisic acid in rice seeds
Nenghui Ye (2012)
10.3724/SP.J.1143.2010.09208
Research advances in methane emission from plants.
Zhang Xiu-jun (2010)
10.1016/j.phytol.2019.08.006
Norbisabolane and bisabolane sesquiterpenoids from the seeds of Angelica keiskei
S. Ohta (2019)
Some Aspects of the Interaction between Cotton Anionic Peroxidases and Verticillium dahliae Kleb .
Nigora Khashimova (2012)
10.1007/s00425-011-1379-y
Oxygen activation at the plasma membrane: relation between superoxide and hydroxyl radical production by isolated membranes
Eiri Heyno (2011)
10.1007/s00344-013-9358-0
Reactive Oxygen Species and Relative Enzyme Activities in the Development of Aerial Roots of Chinese Banyan (Ficus microcarpa)
N. Liu (2013)
10.1016/J.CARBPOL.2011.10.045
Ascorbic acid induced degradation of beta-glucan: Hydroxyl radicals as intermediates studied by spin trapping and electron spin resonance spectroscopy
Audrey M. Faure (2012)
10.1007/s00425-003-1028-1
Evidence for the involvement of cell wall peroxidase in the generation of hydroxyl radicals mediating extension growth
A. Liszkay (2003)
10.2478/s11756-013-0161-y
Reactive oxygen species and seed germination
M. P. Gomes (2013)
10.1016/j.lwt.2020.109324
Phenolic compounds limit or promote oxidative degradation of pectin related to iron-H2O2 ratio
Kévin Vidot (2020)
10.1002/BIOF.5520280202
Plasma membrane‐generated reactive oxygen intermediates and their role in cell growth of plants
P. Schopfer (2006)
10.1016/J.FOODCHEM.2008.03.049
Modification of pectin polysaccharides during ripening of postharvest banana fruit
X. Duan (2008)
10.1002/9780470988565.CH8
Reactive Oxygen Species in Plant Development and Pathogen Defence
M. Jones (2007)
10.1371/journal.pone.0020714
Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation
Xuegui Bai (2011)
10.1016/j.phytochem.2010.06.024
Proteomic and activity profiles of ascorbate-glutathione cycle enzymes in germinating barley embryo.
B. Bønsager (2010)
10.1016/j.biomaterials.2015.11.014
A highly tumor-specific light-triggerable drug carrier responds to hypoxic tumor conditions for effective tumor treatment.
W. Park (2016)
10.1016/j.foodchem.2007.12.041
Effects of reactive oxygen species on cellular wall disassembly of banana fruit during ripening.
G. Cheng (2008)
See more
Semantic Scholar Logo Some data provided by SemanticScholar