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Additive Effects Of Copper And Zinc On Cadmium Toxicity On Phosphatase Activities And ATP Content Of Soil As Estimated By The Ecological Dose (ED50)

G. Renella, A.L.R Ortigoza, Loretta Landi, P. Nannipieri
Published 2003 · Chemistry

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The ecological dose (ED50) of Cd on alkaline and acid phosphatase activity and the ATP content of three contrasting forest soils was measured with or without Cu and Zn to assess the additive toxic effects of these two metals. Soils polluted with Cu and/or Zn were treated with increasing Cd concentrations to give the following metal combinations: Cd, Cd+Cu, Cd+Zn and Cd+Cu+Zn. Alkaline and acid phosphatase activities and ATP content of the three soils were analysed 4 h, 7 and 28 days after the metal additions. The ED50 values were obtained by interpolating the enzyme activities or ATP data with a kinetic model and the goodness of fit was satisfactory. Generally, the ED50 values of both acid and alkaline phosphatase activities for Cd were lower (higher toxicity) with than without Cu and Zn and the effect of Cu and Zn was particularly adverse when these two metals were both added to soils. The alkaline phosphatase was more sensitive in the acid and neutral soil whereas the acid phosphatase was more sensitive in the alkaline soil. Both phosphatase activities and the ATP content were more sensitive in the sandy than in the finer textured soils. The ATP content was less sensitive to the additive effects. Increasing toxicity was observed during the incubation. Analysis of 1 M NH4NO3-extractable Cd, Cu and Zn revealed that Cd competed with Zn for the adsorption sites but not with Cu. However, the lower ED50 values for Cd of the two phosphatase activities and of the ATP content in the presence of heavy metal combinations could be not explained by the heavy metal solubility data. It is concluded that the ED50 may be a sensitive tool for assessing additve toxic effects to soil biochemical parameters.
This paper references
10.2134/JEQ1987.00472425001600020012X
Microbial Mobilization of Cadmium in Soil Under Aerobic and Anaerobic Conditions
P. Chanmugathas (1987)
10.1016/S0038-0717(00)00189-9
The ecological dose value (ED50) for assessing Cd toxicity on ATP content and dehydrogenase and urease activities of soil
J. Moreno (2001)
10.1016/0038-0717(69)90012-1
Use of p-nitrophenyl phosphate for assay of soil phosphatase activity
M. Tabatabai (1969)
The potential use of soil enzymes as indicators of productivity, sustainability and pollution.
P. Nannipieri (1994)
10.1016/S0038-0717(98)00169-2
Simple kinetic approach to determine the toxicity of AS[V] to soil biological properties
T. W. Speir (1999)
10.1016/0038-0717(94)00231-O
A simple kinetic approach to derive the ecological dose value, ED50, for the assessment of Cr(VI) toxicity to soil biological properties
T. W. Speir (1995)
10.1016/S0038-0717(00)00163-2
The influence of the content of heavy metals and molecular weight of humic acids fractions on the activity and stability of urease
C. Marzadori (2000)
10.1007/s003740000205
Influence of cadmium on the metabolic quotient, l- : d-glutamic acid respiration ratio and enzyme activity : microbial biomass ratio under laboratory conditions
Loretta Landi (2000)
10.1007/BF01057585
Application of the “ecological dose” concept to the impact of heavy metals on some microbe-mediated ecologic processes in soil
H. Babich (1983)
Soil Biota: Management in Sustainable Farming Systems
C. Pankhurst (1996)
10.2136/SSSAJ1983.03615995004700060010X
Competitive Complexation of Cd(II) and Cu(II) by Water-Soluble Organic Ligands and Na-Montmorillonite 1
W. P. Inskeep (1983)
10.1046/J.1365-2389.1999.00220.X
General purpose Freundlich isotherms for cadmium, copper and zinc in soils
E. Elzinga (1999)
10.1007/BF00260846
Short- and long-term effects of heavy metals on urease activity in soils
P. Doelman (2004)
10.1007/978-1-4613-8847-0_1
Reactions controlling heavy metal solubility in soils
M. Mcbride (1989)
10.1271/BBB1961.52.1643
Purification and Characterization of Extracellular Alkaline Phosphatase from an Alkalophilic Bacterium
M. Nomoto (1988)
10.1126/SCIENCE.1496397
Uranium bioaccumulation by a Citrobacter sp. as a result of enzymically mediated growth of polycrystalline HUO2PO4.
L. Macaskie (1992)
10.1007/BF00266485
Short- and long-term effects of heavy metals on phosphatase activity in soils: An ecological dose-response model approach
P. Doelman (2004)
Action values for mobile (NH4NO3-extractable) trace elements in soils based on the German national standard DIN 19730
A. Prüess (1997)
10.1016/S0038-0717(97)00231-9
The effect of copper on the activity of cellulase and β-glucosidase in the presence of montmorillonite or Al-montmorillonite
G. Geiger (1998)
10.1007/BF00384432
Effects of lead pollution on different soil enzyme activities
C. Marzadori (2004)
10.1007/BF00193778
Cadmium soil sorption at low concentrations: VI. A model for zinc competition
T. Christensen (1987)
10.1016/0038-0717(95)00005-Y
Cellulase activity of soils: Effect of trace elements
S. Deng (1995)
10.1016/S0038-0717(00)00109-7
Arylamidase activity in soils: effect of trace elements and relationships to soil properties and activities of amidohydrolases
V. Acosta-Martínez (2001)
10.1111/ejss.4_12398
Microbial diversity and soil functions
P. Nannipieri (2003)
10.1016/s0140-6736(01)14139-5
Metals and micro-organisms
M. Hughes (1989)
10.1007/BF00335828
An ecological dose-response model approach to short- and long-term effects of heavy metals on arylsulphatase activity in soil
L. Haanstra (2004)
10.1016/S0038-0717(00)00162-0
Effects of copper on the activity and kinetics of free and immobilized acid phosphatase
Qiaoyun Huang (2000)
10.1146/ANNUREV.BB.21.060192.002301
Structure and mechanism of alkaline phosphatase.
J. Coleman (1992)
10.1007/BF01055564
Immobilization of cadmium by microbial extracellular products
E. Kurek (1991)
10.1007/BF02143194
The use of sigmoidal dose response curves in soil ecotoxicological research
L. Haanstra (2005)
10.1016/0038-0717(82)90099-2
Enzyme activity in soil: Location and a possible role in microbial ecology
R. Burns (1982)
10.1016/0038-0717(90)90022-R
A comparison of methods for measuring ATP in soil
C. Ciardi (1990)
10.1007/BF00017258
Heavy metal pollution and soil enzymatic activity
G. Tyler (2004)
10.1016/S0038-0717(99)00073-5
Microorganisms as metal sorbents: comparison with other soil constituents in multi-compartment systems.
M. Ledin (1999)
10.1016/S0003-2670(97)00717-4
Inhibition behavior of acid phosphatase, phosphodiesterase I and adenosine deaminase as tools for trace metal analysis and speciation
U. Blum (1998)
10.1111/J.1469-8137.1993.TB03796.X
Interactions of fungip with toxic metals
G. M. Gadd (1993)



This paper is referenced by
10.1080/03650340.2010.483592
Does salinity enhance Cd toxicity to soil alkaline phosphatase?
Z. Khanmohammadi (2011)
10.1007/978-3-642-02436-8_11
Effects of Heavy Metals on Soil Enzyme Activities
A. Karaca (2010)
10.1016/J.ECOENV.2006.05.001
Effects of lead contamination on soil enzymatic activities, microbial biomass, and rice physiological indices in soil-lead-rice (Oryza sativa L.) system.
L. S. Zeng (2007)
10.5897/AJMR10.707
Agricultural utilisation of dairy sewage sludge: Its effect on enzymatic activity and microorganisms of the soil environment
M. Frąc (2011)
Effects of Heavy Metal Pollutions on the Ecological Functions of Soil Microbes:A Review
Luo Wei (2010)
MODEL OF PLANT-SOIL COMPLEX TREATED WITH RADOMIR METAL INDUSTRIES WASTE WATER
. Lyubenova (2011)
10.1128/AEM.72.2.1684-1687.2006
Cumulative Effects of Short-Term Polymetal Contamination on Soil Bacterial Community Structure
L. Ranjard (2006)
10.1007/S12665-009-0200-8
Assessment of effects of heavy metals combined pollution on soil enzyme activities and microbial community structure: modified ecological dose–response model and PCR-RAPD
Yang Gao (2010)
10.1109/ICBBE.2008.559
Research on the Woodland Soil Enzyme Activities in Coal Mining Subsidence Area
Xu Hua-shan (2008)
10.1111/JPH.12347
Effects of the Biocontrol Agent Aspergillus flavipes on the Soil Microflora and Soil Enzymes in the Rooting Zone of Pepper Plants Infected with Phytophthora capsici
Wang Hui (2015)
10.3906/BIY-1301-52
Hepatopancreas mitochondria of Mytilus galloprovincialis : effect of zinc ions on mitochondrial bioenergetics and metabolism
A. D. Lisi (2013)
10.1016/j.ecoenv.2014.12.031
Effects of mine wastewater irrigation on activities of soil enzymes and physiological properties, heavy metal uptake and grain yield in winter wheat.
Shou-chen Ma (2015)
10.1016/j.jhazmat.2010.09.019
The biochemical response to different Cr and Cd concentrations in soils amended with organic wastes.
M. Tejada (2011)
10.1007/978-0-387-32964-2_4
Using soil health to assess ecotoxicological impacts of pollutants on soil microflora.
V. Bécaert (2006)
Soil microbiological indices of polluted soils of industrial belts of Jammu, India
Mansoor Rampurawala Ali (2014)
10.5504/BBEQ.2011.0158
Ecotoxicological Assessment Model of Cultural Plant-Soil Complex Treated with Waste Water
Mariyana Lyubenova (2012)
10.1016/S1001-0742(09)60215-1
Effects of plant species coexistence on soil enzyme activities and soil microbial community structure under Cd and Pb combined pollution.
Y. Gao (2010)
10.1631/JZUS.2007.A1157
Effects of Hg and Cu on the activities of soil acid phosphatase
Dongmei Xu (2007)
10.1016/S1002-0160(11)60188-4
Plant Cover and Soil Biochemical Properties in a Mine Tailing Pond Five Years After Application of Marble Wastes and Organic Amendments
R. Zornoza (2012)
10.1016/j.chemosphere.2012.10.107
Carbon mineralization, microbial activity and metal dynamics in tailing ponds amended with pig slurry and marble waste.
R. Zornoza (2013)
10.6001/EKOLOGIJA.V58I2.2524
Effect of zinc and copper on cultivable populations of soil fungi with special reference to entomopathogenic fungi
Dalė Pečiulytė (2012)
10.1016/J.SOILBIO.2010.06.018
Relative sensitivity of different soil biological properties to zinc
Damiano Coppolecchia (2011)
10.1007/s11368-016-1529-8
Evaluation of enzyme activities in long-term polluted soils with mine tailing deposits of San Luis Potosí, México
Á. Martínez-Toledo (2016)
10.1016/j.chemosphere.2008.04.042
Assessment of chemical, biochemical and ecotoxicological aspects in a mine soil amended with sludge of either urban or industrial origin.
P Alvarenga (2008)
10.1016/J.APSOIL.2009.01.001
Composition, biomass and activity of microflora, and leaf yields and foliar elemental concentrations of lettuce, after in situ stabilization of an arsenic-contaminated soil
J. Ascher (2009)
10.1016/j.scitotenv.2009.05.017
Microbiological polyphasic approach for soil health evaluation in an Italian polluted site.
A. Fabiani (2009)
10.1080/00958972.2016.1167885
Pharmacological activities of a propylthiouracil compound structurally modified by coordination with copper(II)
Nora M. Urquiza (2016)
Aging Effects On Partitioning Coefficients of Cd, Cu, and Zn in Metal-spiked Soils
B. Kim (2008)
Chapter 9 Role of Phosphatase Enzymes in Soil
P. Nannipieri (2010)
10.1016/j.chemosphere.2017.11.082
Revitalisation of metal-contaminated, EDTA-washed soil by addition of unpolluted soil, compost and biochar: Effects on soil enzyme activity, microbial community composition and abundance.
Anela Kaurin (2018)
10.1007/s00244-012-9842-8
Metal Contamination Disturbs Biochemical and Microbial Properties of Calcareous Agricultural Soils of the Mediterranean Area
A. Santiago-Martín (2012)
10.1007/s00374-004-0769-8
Spatial variability of enzyme activities in a 100-year old long-term field experiment
Livia Boehme (2004)
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