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DOI: 10.1016/S0076-6879(96)64008-7

In Organello RNA Synthesis System From Mammalian Liver And Brain.

J. Enríquez, A. Pérez-Martos, M. López-Pérez, Julio Montoya
Published 1996 · Medicine, Biology

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Publisher Summary This chapter describes a system to study RNA synthesis by using isolated mitochondria from mammalian brain and liver. In this system, mtDNA transcription and RNA processing are supported in a way that closely reproduces the vivo process. In vivo study of the regulation of mtDNA expression is hampered by the inherent difficulty of manipulating mammalian mitochondrial genes in vivo and of discriminating between direct regulatory phenomena on mtDNA transcription and RNA metabolism and secondary actions dependent on nuclear control. Based on this experimental approach, several attempts are made to apply this system to the study of mitochondrial RNA synthesis in mammalian organs. However, under the incubation conditions described for the organelles isolated from HeLa cells, the mitochondria isolated from the mammalian tissue do not reproduce closely the in vivo processes of mtDNA transcription and RNA processing and the efficiency of transcription is very low.
This paper references
10.1016/0014-5793(91)80197-B
Saturation of the processing of newly synthesized rRNA in isolated brain mitochondria
J. Enríquez (1991)
10.5555/URI:PII:0022214356900646
A simple ultraviolet spectrophotometric method for the determination of protein.
W. Waddell (1956)
10.1146/ANNUREV.CB.04.110188.001445
The biogenesis of mitochondria.
Gottfried Schatz (1970)
10.1111/J.1432-1033.1989.TB14638.X
Control of mitochondrial transcription by thyroid hormone.
A. Mutvei (1989)
10.1038/290465A0
Distinctive features of the 5′-terminal sequences of the human mitochondrial mRNAs
Julio Montoya (1981)
10.1016/0014-5793(92)80639-X
Specific increase of a mitochondrial RNA transcript in chronic ethanol‐fed rats
J. Enríquez (1992)
10.1016/0020-711X(88)90330-8
RNA synthesis in isolated mitochondria from brain cortex, cerebellum and stem: evidence of different transcriptional rates.
J. Enríquez (1993)
10.1016/0022-2836(78)90241-3
Fractionation of mitochondrial RNA from HeLa cells by high-resolution electrophoresis under strongly denaturing conditions.
F. Amalric (1978)
10.1016/S0006-291X(05)80233-5
Reduced synthesis of mtRNA in isolated mitochondria of senescent rat brain.
P. Fernández-Silva (1991)
10.1128/MCB.4.8.1605
Highly efficient RNA-synthesizing system that uses isolated human mitochondria: new initiation events and in vivo-like processing patterns.
G. Gaines (1984)
10.1016/S0022-2836(84)80017-0
Intercalating drugs and low temperatures inhibit synthesis and processing of ribosomal RNA in isolated human mitochondria.
G. Gaines (1984)
10.1093/NAR/22.10.1861
Highly efficient DNA synthesis in isolated mitochondria from rat liver.
J. Enríquez (1994)
10.1016/0092-8674(83)90145-9
The pattern of transcription of the human mitochondrial rRNA genes reveals two overlapping transcription units
Julio Montoya (1983)
A simple procedure for recovering the denaturing effect of methylmercury in agarose gel electrophoresis.
P. Fernández-Silva (1992)
10.1038/290470A0
tRNA punctuation model of RNA processing in human mitochondria
D. Ojala (1981)
10.1146/ANNUREV.BI.45.070176.003133
Processing of RNA.
R. P. Perry (1976)
10.1016/S0005-2728(05)80235-X
Effect of chronic ethanol consumption on hepatic mitochondrial transcription and translation.
W. Coleman (1991)
10.1128/MCB.1.6.497
Synthesis and turnover of mitochondrial ribonucleic acid in HeLa cells: the mature ribosomal and messenger ribonucleic acid species are metabolically unstable.
R. Gelfand (1981)
10.1016/s0021-9258(19)75725-1
Markedly different ATP requirements for rRNA synthesis and mtDNA light strand transcription versus mRNA synthesis in isolated human mitochondria.
G. Gaines (1987)



This paper is referenced by
10.1074/jbc.M107482200
The 2–5A/RNase L/RNase L Inhibitor (RNI) Pathway Regulates Mitochondrial mRNAs Stability in Interferon α-treated H9 Cells*
F. Le Roy (2001)
10.1128/MCB.24.22.9823-9834.2004
Architectural Role of Mitochondrial Transcription Factor A in Maintenance of Human Mitochondrial DNA
T. Kanki (2004)
10.1093/NAR/GKG717
Import of mitochondrial transcription factor A (TFAM) into rat liver mitochondria stimulates transcription of mitochondrial DNA.
H. L. Garstka (2003)
10.1016/S0076-6879(96)64007-5
In organello RNA synthesis system from HeLa cells.
G. Gaines (1996)
10.1016/j.ab.2009.02.040
Isolation of functional pure mitochondria by superparamagnetic microbeads.
Hue-Tran Hornig-Do (2009)
10.1016/B978-012164730-8/50082-4
Isolation of Mitochondria from Mammalian Tissues and Cultured Cells
Erika Fernández-Vizarra (2006)
10.1016/j.mito.2009.12.148
Isolation of mitochondria for biogenetical studies: An update.
E. Fernández-Vizarra (2010)
10.1016/S1046-2023(02)00034-8
Isolation of biogenetically competent mitochondria from mammalian tissues and cultured cells.
E. Fernández-Vizarra (2002)
10.1006/BBRC.1998.9703
Reduced mitochondrial DNA transcription in senescent rat heart.
A. Andreu (1998)
10.1016/S0014-5793(00)01768-3
Iron(II) induces changes in the conformation of mammalian mitochondrial DNA resulting in a reduction of its transcriptional rate
J. Asín (2000)
10.1016/S1567-7249(01)00010-1
Chronic exposure of neural cells to elevated intracellular sodium decreases mitochondrial mRNA expression.
K. Chandrasekaran (2001)
10.1196/annals.1293.009
Regulation and Role of the Mitochondrial Transcription Factor in the Diabetic Rat Heart
Y. Nishio (2004)
10.1016/j.cell.2007.05.046
MTERF3 Is a Negative Regulator of Mammalian mtDNA Transcription
C. B. Park (2007)
10.1152/AJPENDO.00255.2001
Reduced activity of mtTFA decreases the transcription in mitochondria isolated from diabetic rat heart.
A. Kanazawa (2002)
10.1128/MCB.19.1.657
Direct Regulation of Mitochondrial RNA Synthesis by Thyroid Hormone
J. Enríquez (1999)
10.1111/febs.15342
Messenger RNA delivery to mitoribosomes: hints from a bacterial toxin.
F. Bruni (2020)
10.1016/S0091-679X(01)65024-6
Diagnostic assays for defects in mitochondrial DNA replication and transcription in yeast and human cells.
B. Seidel-Rogol (2001)
10.1042/BJ20071615
Cisplatin-mediated impairment of mitochondrial DNA metabolism inversely correlates with glutathione levels.
N. Garrido (2008)
10.1023/A:1010812128765
ATP synthesis is coupled to rat liver mitochondrial RNA synthesis
S. Dasgupta (2004)
10.1016/0928-4346(96)00289-7
Human liver mitochondrial RNA synthesized in isolated organelles
A. Pérez-Martos (1996)
10.1007/978-3-642-39426-3_4
Mitochondrial Targeting of RNA and Mitochondrial Translation
I. Tarassov (2013)
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