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Mapping Promoters In Displacement-loop Region Of Vertebrate Mitochondrial DNA.
Published 1996 · Biology, Medicine
Publisher Summary This chapter discusses a general strategy for locating the major promoters in the D-loop region of the mitochondrial genome of vertebrate organisms. It proposes two approaches for the isolation of a D-loop region-containing DNA fragment that utilize conserved nucleotide sequences occurring within or near the D-loop region of vertebrates. Once in hand, promoters can be precisely located on this template using a crude mitochondrial RNA (mtRNA) polymerase preparation to assay promoter activity in an in vitro transcription reaction. This chapter discusses the aspects unique to analyzing mitochondrial promoters. It describes the methods that are used to locate D-loop region promoters of human and mouse mitochondrial DNAs (mtDNAs) as models for this type of analysis.