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Mechanism Of Granulosa Cell Death During Follicular Atresia Depends On Follicular Size.
Israel Alonso-Pozos, A. M. Rosales-Torres, A. Ávalos-Rodríguez, M. Vergara-Onofre, A. Rosado-García
Published 2003 · Biology, Medicine
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Changes in granulosa cell lysosomal and mitochondrial functions in relation to follicular size and to the stage of atresia were studied by fluorescent emission spectra and intensity using flow cytometry. Antral follicles were grouped by size in two groups: small, 3-6 mm and large, >6mm in diameter, and classified into three stages of atresia: non-atretic, initially atretic and advanced atretic. Differences in Rhodamine 123 (Rh123) and Acridine Orange (AO) fluorescent intensity indicated that changes in mitochondrial function are the primary mechanism of granulosa cell death in atretic follicles 3-6 mm in diameter, while its role in granulosa cell death in >6 mm atretic follicles seemed to be less important. However, modifications in lysosomal function (shown by a decrease in fluorometric intensity of AO incubated granulosa cells) were mainly associated with cell death in large atretic follicles. Our results support the hypothesis that the pathway of granulosa cell death during follicular atresia depends on the state of energy metabolism or on the production of hypoxic conditions related to follicular size. Changes in mitochondrial membrane potential and production of permeability transition pores were the main changes found in small follicles, while lysosomal function destabilization seemed to be the major cause of granulosa cell death during atresia in large follicles.
This paper references
Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis).
Z. Darzynkiewicz (1997)
A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1).
A. Cossarizza (1993)
Development of follicles in the mammalian ovary.
A. Hirshfield (1991)
Glutamate-induced neuronal death: A succession of necrosis or apoptosis depending on mitochondrial function
M. Ankarcrona (1995)
Comparative morphometry and steroidogenic function of antral ovine follicles destined for ovulation or atresia.
W J Murdoch (1992)
Induction of cell death by the lysosomotropic detergent MSDH
W. Li (2000)
Lethal hydrogen peroxide toxicity involves lysosomal iron-catalyzed reactions with membrane damage.
U. Brunk (1995)
Caspase-mediated apoptosis in AK-5 tumor cells: a cell-free study using peptide inhibitors and antisense strategy.
R. Anjum (1997)
The mitochondrial apoptosome: a killer unleashed by the cytochrome seas.
C. Adrain (2001)
Follicular dynamics in sheep and cattle
M. Driancourt (1991)
Induction of apoptosis as well as necrosis by hypoxia and predominant prevention of apoptosis by Bcl-2 and Bcl-XL.
S. Shimizu (1996)
Apoptosis in atretic ovarian follicles is associated with selective decreases in messenger ribonucleic acid transcripts for gonadotropin receptors and cytochrome P450 aromatase.
J. Tilly (1992)
Cell death: the significance of apoptosis.
A. Wyllie (1980)
Semiempirical potential surfaces for the alkali hydrogen‐halide reactions
M. Shapiro (1979)
Commentary. Lysosomotropic agents.
C. de Duve (1974)
Studies on follicular atresia: role of gonadotropins and gonadal steroids in regulating cathepsin-D activity of preovulatory follicles in the rat
N. Dhanasekaran (1989)
Analysis of atresia in bovine follicles using different methods: flow cytometry, enzyme-linked immunosorbent assay, and classic histology.
P. Blondin (1996)
Activity, compartmentation and subcellular distribution of lysosomal enzymes during follicular maturation in the goat
Ballesteros Lm (1992)
Commitment to apoptosis is associated with changes in mitochondrial biogenesis and activity in cell lines conditionally immortalized with simian virus 40.
J. L. Vayssière (1994)
Regulation of a novel pathway for cell death by lysosomal aspartic and cysteine proteinases
K. Isahara (1999)
Cytofluorometric quantitation of acridine orange uptake by cultured cells.
I. Rundquist (1984)
Apoptosis: Identification of dying cells
C. Renvoizé (2004)
Proteolytic activity degrading insulin-like growth factor-binding protein-2, -3, -4, and -5 in healthy growing and atretic follicles in the pig ovary.
N. Besnard (1997)
Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures.
E. Bonfoco (1995)
Control of apoptosis by the cellular ATP level
C. Richter (1996)
Assays of cell viability: discrimination of cells dying by apoptosis.
Z. Darzynkiewicz (1994)
Ovarian follicle macrophages: is follicular atresia in the immature rat a macrophage-mediated event?
F. Gaytán (1998)
Interaction of rhodamine 123 with living cells studied by flow cytometry.
Z. Darzynkiewicz (1982)
Mitochondrial Implication in Accidental and Programmed Cell Death: Apoptosis and Necrosis
N. Zamzami (1997)
Biochemical identification of apoptosis (programmed cell death) in granulosa cells: evidence for a potential mechanism underlying follicular atresia.
F. Hughes (1991)
Intracellular Adenosine Triphosphate (ATP) Concentration: A Switch in the Decision Between Apoptosis and Necrosis
M. Leist (1997)
Localization of mitochondria in living cells with rhodamine 123.
L. Johnson (1980)
Evidence for alternative pathways of granulosa cell death in healthy and slightly atretic bovine antral follicles.
I. L. van Wezel (1999)
Subtypes of active cell death in the granulosa of ovarian atretic follicles in the quail (Coturnix coturnix japonica).
K. D'herde (1996)
Supravital exposure to propidium iodide identifies apoptotic cells in the absence of nucleosomal DNA fragmentation.
L. Zamai (1996)
Different prooxidant levels stimulate growth, trigger apoptosis, or produce necrosis of insulin-secreting RINm5F cells. The role of intracellular polyamines.
J. Dypbukt (1994)
Metalloproteinase activity during growth, maturation and atresia in the ovarian follicles of the goat.
R. García (1997)
Induction of Apoptotic Program in Cell-Free Extracts: Requirement for dATP and Cytochrome c
X. Liu (1996)
Tumor necrosis factor-induced hepatocyte apoptosis precedes liver failure in experimental murine shock models.
M. Leist (1995)
Flow cytometric determination of degraded deoxyribonucleic acid in granulosa cells to identify atretic follicles during preovulatory maturation in the pig.
H. D. Guthrie (1994)
Proteolytic and antiproteolytic activities in goat antral follicles
A. M. Rosales (1990)
Lysosomal proton pump activity: supravital cell staining with acridine orange differentiates leukocyte subpopulations.
F. Traganos (1994)
Size-frequency analysis of atresia in cycling rats.
A. Hirshfield (1988)
The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy.
J. Lemasters (1998)
Reduction in mitochondrial potential constitutes an early irreversible step of programmed lymphocyte death in vivo
N. Zamzami (1995)
Multiparametric study of atresia in ewe antral follicles: Histology, flow cytometry, internucleosomal DNA fragmentation, and lysosomal enzyme activities in granulosa cells and follicular fluid
A. M. Rosales-Torres (2000)
Apoptosis and necrosis: Intracellular ATP level as a determinant for cell death modes
Y. Tsujimoto (1997)
Cell death: Current difficulties in discriminating apoptosis from necrosis in the context of pathological processes in vivo
A. Columbano (1995)
This paper is referenced by
Changes in the metabolic profile and body weight of pre‐pubertal gilts during prolonged monotonic exposure to low doses of zearalenone and deoxynivalenol
M. Gajęcka (2017)
Effects of melatonin on follicular atresia and granulosa cell apoptosis in the porcine
Yamei He (2016)
Expression of Hyaluronan Synthase 1 and Distribution of Hyaluronan During Follicular Atresia in Pig Ovaries1
Yuko Miyake (2009)
Endoplasmic reticulum stress is involved in granulosa cell apoptosis during follicular atresia in goat ovaries
P. Lin (2012)
Association of luteal cell degeneration and progesterone deficiency with lysosomal storage disorder mucolipidosis type IV in Mcoln1 -/- mouse model†
Zidao Wang (2019)
Morphological and Hormonal Identification of Porcine Atretic Follicles and Relationship Analysis of Hormone Receptor Levels During Granulosa Cell Apoptosis In vivo
D. Yu (2014)
Roles of SIRT1 in granulosa cell apoptosis during the process of follicular atresia in porcine ovary.
F. Zhao (2014)
Deoxynivalenol in the Gastrointestinal Tract of Immature Gilts under per os Toxin Application
Agnieszka Waśkiewicz (2014)
Two novel techniques to detect follicles in human ovarian cortical tissue.
R. Soleimani (2006)
Effects of 5-Aza-2'-deoxycytidine on hormone secretion and epigenetic regulation in sika deer ovarian granulosa cells.
Xiumin Chen (2020)
Diabetes Induces Abnormal Ovarian Function via Triggering Apoptosis of Granulosa Cells and Suppressing Ovarian Angiogenesis
Y. Wu (2017)
Effects of tributyltin on gonad and brood pouch development of male pregnant lined seahorse (Hippocampus erectus) at environmentally relevant concentrations.
Lu Tang (2020)
Suppression of miR-1197-3p attenuates H2O2-induced apoptosis of goat luteinized granulosa cells via targeting PPARGC1A.
G. Zhang (2019)
Necrosis and necroptosis in germ cell depletion from mammalian ovary
G. Chaudhary (2019)
[Role of Fas/FasL on apoptosis of porcine follicular granulosa cells derived from isolated follicles during culture in vitro].
P. Lin (2010)
Apoptosis en la atresia folicular y la regresión del cuerpo lúteo. Revisión
A. M. Torres (2008)
Distribution and Y397 phosphorylation of focal adhesion kinase on follicular development in the mouse ovary
M. Sakurai (2011)
The effects of dietary deoxynivalenol (DON) on selected blood biochemical and hematological parameters in pre-pubertal gilts.
Ł. Zielonka (2015)
Toxic Effects of Maternal Zearalenone Exposure on Uterine Capacity and Fetal Development in Gestation Rats
Yuanyuan Zhang (2014)
Influence of chronic administration of zearalenone on the processes of apoptosis in the porcine ovary
K. Wąsowicz (2018)
Growth performance, selected blood biochemical parameters and body weights of pre‐pubertal gilts fed diets supplemented with different doses of zearalenone (ZEN)
Anna Rykaczewska (2018)
Histone deacetylase inhibitor stimulates E2 and P4 secretion in sika deer ovarian granulosa cells at a moderate dose
Mingjie Xing (2018)
Knock-down of apoptosis inducing factor gene protects endoplasmic reticulum stress-mediated goat granulosa cell apoptosis.
Diqi Yang (2017)
Does 2-hydroxyflutamide inhibit apoptosis in porcine granulosa cells? - An in vitro study.
M. Duda (2012)
Identification of the JY-1 in sika deer and its effect on granulosa cell culture in vitro.
Xiumin Chen (2019)
Functional status of STAT3 and MAPK3/1 signaling pathways in granulosa cells during bovine follicular deviation.
B. Gasperin (2015)
Mycotoxin levels in the digestive tissues of immature gilts exposed to zearalenone and deoxynivalenol
M. Gajęcka (2018)
Developing embryo technologies for the eland antelope (Taurotragus oryx)
G. Wirtu (2004)
Effects of follicular size and FSH on granulosa cell apoptosis and atresia in porcine antral follicles
P. Lin (2010)
Regulação da divergência folicular in vivo: uma abordagem molecular
B. Gasperin (2012)
FLUOROMETRIC STUDY OF RABBIT SPERM HEAD MEMBRANE PHOSPHOLIPID ASYMMETRY DURING CAPACITATION AND ACROSOME REACTION USING ANNEXIN-V FITC
A. Ávalos-Rodríguez (2004)
FOLLICULAR DEVELOPMENT IN DOMESTIC RUMINANTS
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