← Back to Search
Photoencapsulation Of Osteoblasts In Injectable RGD-modified PEG Hydrogels For Bone Tissue Engineering.
J. Burdick, K. Anseth
Published 2002 · Materials Science, Medicine
Download PDFAnalyze on Scholarcy
Poly(ethylene glycol) (PEG) hydrogels were investigated as encapsulation matrices for osteoblasts to assess their applicability in promoting bone tissue engineering. Non-adhesive hydrogels were modified with adhesive Arg-Gly-Asp (RGD) peptide sequences to facilitate the adhesion, spreading, and, consequently, cytoskeletal organization of rat calvarial osteoblasts. When attached to hydrogel surfaces, the density and area of osteoblasts attached were dramatically different between modified and unmodified hydrogels. A concentration dependence of RGD groups was observed, with increased osteoblast attachment and spreading with higher RGD concentrations, and cytoskeleton organization was seen with only the highest peptide density. A majority of the osteoblasts survived the photoencapsulation process when gels were formed with 10% macromer, but a decrease in osteoblast viability of approximately 25% and 38% was seen after 1 day of in vitro culture when the macromer concentration was increased to 20 and 30wt%, respectively. There was no statistical difference in cell viability when peptides were added to the network. Finally, mineral deposits were seen in all hydrogels after 4 weeks of in vitro culture, but a significant increase in mineralization was observed upon introduction of adhesive peptides throughout the network.
This paper references
Osteoblast population migration characteristics on substrates modified with immobilized adhesive peptides.
K. Dee (1999)
The ingrowth of new bone tissue and initial mechanical properties of a degrading polymeric composite scaffold.
M. Yaszemski (1995)
Crosslinked polyanhydrides for use in orthopedic applications: degradation behavior and mechanics.
D. Muggli (1999)
Transtissue photopolymerization of poly(vinyl alcohol) hydrogels. In: Stokke BT, Elgsaeter, editors. Chemical and physical network formation and control
S Bryant (1999)
Bioerodible hydrogels based on photopolymerized poly(ethylene glycol)-co-poly(.alpha.-hydroxy acid) diacrylate macromers
A. Sawhney (1993)
Thermal aspects of self-curing polymethylmethacrylate.
C. Jefferiss (1975)
Thermo-responsive peptide-modified hydrogels for tissue regeneration.
R. A. Stile (2001)
Osteoblast function on synthetic biodegradable polymers.
S. L. Ishaug (1994)
The effects of scaffold thickness on tissue engineered cartilage in photocrosslinked poly(ethylene oxide) hydrogels.
S. Bryant (2001)
Surface and bulk modifications to photocrosslinked polyanhydrides to control degradation behavior.
A. K. Burkoth (2000)
Biosynthetic bone grafting.
J. Lane (1999)
Osteoblast migration characteristics on substrates modified with immobilized adhesive peptides
KC Dee (1999)
Injectable biodegradable materials for orthopedic tissue engineering.
J. Temenoff (2000)
The effect of peptide surface density on mineralization of a matrix deposited by osteogenic cells.
A. Rezania (2000)
In vitro degradation of a poly(propylene fumarate)/β-tricalcium phosphate composite orthopaedic scaffold
S. Peter (1997)
Smooth muscle cell growth in photopolymerized hydrogels with cell adhesive and proteolytically degradable domains: synthetic ECM analogs for tissue engineering.
B. Mann (2001)
Covalent surface immobilization of Arg-Gly-Asp- and Tyr-Ile-Gly-Ser-Arg-containing peptides to obtain well-defined cell-adhesive substrates.
S. Massia (1990)
Bone graft materials. An overview of the basic science.
T. Bauer (2000)
Crosslinking characteristics of an injectable poly(propylene fumarate)/β‐tricalcium phosphate paste and mechanical properties of the crosslinked composite for use as a biodegradable bone cement
S. Peter (1999)
Biomimetic Peptide Surfaces That Regulate Adhesion, Spreading, Cytoskeletal Organization, and Mineralization of the Matrix Deposited by Osteoblast‐like Cells
A. Rezania (1999)
Alginate hydrogels as synthetic extracellular matrix materials.
J. Rowley (1999)
Incorporation of adhesion peptides into nonadhesive hydrogels useful for tissue resurfacing.
D. Hern (1998)
Mini‐review: Proactive biomaterials and bone tissue engineering
K. Dee (1996)
Transtissue photopolymerization of poly ( vinyl alcohol ) hydrogels
Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro
S. Bryant (2000)
Conversion and temperature profiles during the photoinitiated polymerization of thick orthopaedic biomaterials.
J. Burdick (2001)
Crosslinking characteristics of an injectable poly(propylene fumarate)/beta-tricalcium phosphate paste and mechanical properties of the crosslinked composite for use as a biodegradable bone cement.
S. Peter (1999)
Transdermal photopolymerization for minimally invasive implantation.
J. Elisseeff (1999)
This paper is referenced by
Development of Biomimetic Hydrogels as Cell-Laden Devices for Muscle Regeneration
Róisín Holmes (2017)
Synthesis and properties of photo-crosslinked mixed-macromer networks
Erwin Zant (2015)
Direct Laser Writing
Sascha Engelhardt (2013)
Influence of three-dimensional hyaluronic acid microenvironments on mesenchymal stem cell chondrogenesis.
C. Chung (2009)
Modeling the Process of Fabricating Cell-Encapsulated Tissue Scaffolds and the Process-Induced Cell Damage
X. Tian (2013)
A novel star PEG-derived surface coating for specific cell adhesion.
J. Groll (2005)
Effects of neighboring sulfides and pH on ester hydrolysis in thiol-acrylate photopolymers.
Amber E. Rydholm (2007)
The Chemical Modification of Chitosan Films for Improved Hemostatic and Bioadhesive Properties.
Mai Yamazaki (2008)
The effects of monoacrylated poly(ethylene glycol) on the properties of poly(ethylene glycol) diacrylate hydrogels used for tissue engineering.
J. Beamish (2010)
Pluripotent Stem Cells: Differentiation Potential and Therapeutic Efficacy for Cartilage Repair
W. Toh (2016)
3D Co-Culture with Vascular Cells Supports Long-Term Hepatocyte Phenotype and Function In Vitro
Asli Z. Unal (2018)
Hyaluronic acid hydrogel scaffolds with a triple degradation behavior for bone tissue engineering.
N. Cui (2015)
Polymeric Biomaterials Based on Polylactide, Chitosan and Hydrogels in Medicine
A. Mitrović (2018)
Synthetic hydrogel niches that promote hMSC viability.
C. Nuttelman (2005)
Injectable hydrogels for bone and cartilage repair.
A. A. Amini (2012)
Chondrogenesis in injectable enzymatically crosslinked heparin/dextran hydrogels.
R. Jin (2011)
Production of a safer, osteogenic, tissue engineered bone allograft
C. Smith (2015)
Improving mechanical properties of injectable polymers and composites
Y. Qiu (2011)
Heterogeneity and its Influence on the Properties of Difunctional Poly(ethylene glycol) Hydrogels: Structure and Mechanics
P. M. Molina (2015)
Combining Osteochondral Stem Cells and Biodegradable Hydrogels for Bone Regeneration
M. Bongio (2013)
Adsorption of a cell-adhesive oligopeptide on polymer surfaces irradiated by ion beams.
C. Satriano (2005)
Hydrogels as extracellular matrices for skeletal tissue engineering: state-of-the-art and novel application in organ printing.
N. E. Fedorovich (2007)
The effects of cell-matrix interactions on encapsulated beta-cell function within hydrogels functionalized with matrix-derived adhesive peptides.
Laney M Weber (2007)
Synthesis and Characterization of Novel Fluorescent Injectable Micro-Carriers for Tissue Regeneration
A. Arora (2014)
Microporous cell‐laden hydrogels for engineered tissue constructs
J. Park (2010)
Mediating specific cell adhesion to low-adhesive diblock copolymers by instant modification with cyclic RGD peptides.
E. Lieb (2005)
Poly(ethylene glycol) hydrogels formed by thiol-ene photopolymerization for enzyme-responsive protein delivery.
A. A. Aimetti (2009)
Investigating the atmospheric pressure plasma jet modification of a photo-crosslinkable hydrogel
Inés Hamouda (2020)
Osteodifferentiation of Human Preadipocytes Induced by Strontium Released from Hydrogels
V. Nardone (2012)
Scaffolds in Dental Tissue Engineering: A Review
A. Farzin (2019)
Regeneration of tooth pulp and dentin : trends and advances
Sarang Sharma (2010)
25th anniversary article: Designer hydrogels for cell cultures: a materials selection guide.
J. Thiele (2014)See more