Online citations, reference lists, and bibliographies.
← Back to Search

Induction Of Granulocytic Differentiation In Acute Promyelocytic Leukemia Cells (HL-60) By Water-soluble Chitosan Oligomer.

H. Pae, W. G. Seo, N. Y. Kim, G. S. Oh, G. E. Kim, Y. H. Kim, H. Kwak, Y. Yun, C. D. Jun, H. Chung
Published 2001 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Water-soluble chitosan oligomer (WSCO) has been reported to have anticancer activity, immuno-enhancing effect and antimicrobial activity. However, other biological activities are unknown. Herein, we have shown that WSCO is able to inhibit proliferation of human leukemia HL-60 cells and induce these cells to differentiate. Treatment with WSCO for 4 days resulted in a concentration-dependent reduction in HL-60 cell growth as measured by cell counting and MTT assay. This effect was accompanied by a marked increase in the proportion of G(0)/G(1) cells as measured by flow cytometry. WSCO also induced differentiation of the cells as measured by phorbol ester-dependent reduction of NBT, morphological changes as examined by Wright-Giemsa staining and expression of CD11b but not of CD14 as analysed by flow cytometry, indicating differentiation of HL-60 cells toward granulocyte-like cells. A combination of low dose of WSCO with all-trans retinoic acid, a differentiating agent toward granulocyte-like cells, exhibited a synergistic effect on the differentiation. In addition, treatment of HL-60 cells with WSCO for 6 or 8 days resulted in the induction of apoptosis as assayed qualitatively by agarose gel electrophoresis and quantitatively by Annexin V technique using flow cytometry. Collectively, there is a potential for WSCO in the treatment of myeloid leukemia.
This paper references
10.1038/356397A0
Social controls on cell survival and cell death
M. Raff (1992)
10.1182/BLOOD.V82.7.2175.BLOODJOURNAL8272175
Resistance to all-trans retinoic acid (ATRA) therapy in relapsing acute promyelocytic leukemia: study of in vitro ATRA sensitivity and cellular retinoic acid binding protein levels in leukemic cells.
L. Delva (1993)
10.1016/S0008-6215(00)90359-8
Antitumor effect of hexa-N-acetylchitohexaose and chitohexaose.
K. Suzuki (1986)
10.1002/HON.2900080109
The relation between hypocholesterolemia and degree of maturation in acute myeloid leukemia
L. E. Zyada (1990)
10.1016/0008-6215(95)00288-X
A novel method for chemo-enzymatic synthesis of elicitor-active chitosan oligomers and partially N-deacetylated chitin oligomers using N-acylated chitotrioses as substrates in a lysozyme-catalyzed transglycosylation reaction system.
K. Akiyama (1995)
10.4049/jimmunol.164.4.2110
Activated Macrophages Direct Apoptosis and Suppress Mitosis of Mesangial Cells1
J. Duffield (2000)
10.1182/blood.v65.2.484.484
Myeloperoxidase: its structure and expression during myeloid differentiation.
H. Koeffler (1985)
10.1248/CPB.36.784
Growth-inhibitory effect of hexa-N-acetylchitohexaose and chitohexaose against Meth-A solid tumor.
A. Tokoro (1988)
10.1182/BLOOD.V78.6.1413.BLOODJOURNAL7861413
A clinical and experimental study on all-trans retinoic acid-treated acute promyelocytic leukemia patients.
Z. Chen (1991)
10.1002/(SICI)1097-4636(199705)35:2<165::AID-JBM4>3.0.CO;2-L
Polylysine induces changes in membrane electrical properties of K562 cells.
M. Santini (1997)
10.1111/j.1365-2249.1990.tb08110.x
HL‐60 cells induced to differentiate towards neutrophils subsequently die via apoptosis
S. Martin (1990)
10.1093/JNCI/67.5.1025
Induction of differentiation of human promyelocytic leukemia cells (HL-60) by nucleosides and methotrexate.
A. Bodner (1981)
10.1016/S0168-3659(98)00097-2
Chitosan and depolymerized chitosan oligomers as condensing carriers for in vivo plasmid delivery.
F. Maclaughlin (1998)
10.1016/0014-5793(93)80020-U
Cell cycle phase‐dependent effect of retinoic acid on the induction of granulocytic differentiation in HL‐60 promyelocytic leukemia cells
E. K. Hui (1993)
10.1016/S0928-0987(99)00032-9
Chitosans as absorption enhancers of poorly absorbable drugs. 3: Influence of mucus on absorption enhancement.
N. G. Schipper (1999)
10.1016/0192-0561(88)90463-8
Growth-inhibitory effect of hexa-N-acetylchitohexaose (NACOS-6) and Chitohexaose (COS-6) against meth-a solid tumor
M. Suzuki (1988)
Discovery of natural product chemopreventive agents utilizing HL-60 cell differentiation as a model.
N. Suh (1995)
10.1111/J.1349-7006.1990.TB02559.X
Antimetastatic and Growth‐inhibitory Effects of N‐Acetylchitohexaose in Mice Bearing Lewis Lung Carcinoma
K. Tsukada (1990)
10.1016/S0145-2126(98)00174-X
Acridones as inducers of HL-60 cell differentiation.
S. Kawaii (1999)
10.1093/AJCN/33.4.787
A novel use of chitosan as a hypocholesterolemic agent in rats.
M. Sugano (1980)
10.1182/BLOOD.V76.9.1704.BLOODJOURNAL7691704
All-trans retinoic acid as a differentiation therapy for acute promyelocytic leukemia. I. Clinical results.
S. Castaigne (1990)
10.1111/j.1348-0421.1989.tb01983.x
Protective Effect of N‐Acetyl Chitohexaose on Listeria monocytogenes Infection in Mice
A. Tokoro (1989)
10.1016/0145-2126(94)00061-E
Expression of cell surface antigens during the differentiation of HL-60 cells induced by 1,25-dihydroxyvitamin D3, retinoic acid and DMSO.
D. Brackman (1995)
10.1016/S0145-2126(98)00053-8
Regulation of the expression of enzymes involved in the replication of DNA in chemically-induced granulocytic differentiation of HL-60 leukemia cells.
Y. Chen (1998)
10.1016/S1387-2656(08)70012-7
Chitin biotechnology applications.
S. Hirano (1996)
10.1016/S0145-2126(98)00096-4
Boswellic acid acetate induces differentiation and apoptosis in leukemia cell lines.
Y. Jing (1999)
10.1172/JCI113970
Macrophage phagocytosis of aging neutrophils in inflammation. Programmed cell death in the neutrophil leads to its recognition by macrophages.
J. Savill (1989)
The level of CRABP-I expression influences the amounts and types of all-trans-retinoic acid metabolites in F9 teratocarcinoma stem cells.
J. Boylan (1992)
10.1016/0378-5173(92)90353-4
In vitro evaluation of mucoadhesive properties of chitosan and some other natural polymers
C. Lehr (1992)
10.1016/S0271-5317(98)00091-8
Chitin-chitosan: Properties, benefits and risks
S. Koide (1998)
10.1016/0145-2126(95)00036-N
Leukemia cell lines: in vitro models for the study of acute promyelocytic leukemia.
H. Drexler (1995)
10.1016/0014-4827(82)90105-7
Retinoic acid enhances colony-stimulating factor-induced clonal growth of normal human myeloid progenitor cells in vitro.
D. Douer (1982)
10.1182/BLOOD.V79.2.299.BLOODJOURNAL792299
Continuous treatment with all-trans retinoic acid causes a progressive reduction in plasma drug concentrations: implications for relapse and retinoid "resistance" in patients with acute promyelocytic leukemia.
J. Muindi (1992)
10.1016/S0378-5173(96)04801-6
Interactions between liposomes and chitosan II : effect of selected parameters on aggregation and leakage
I. Henriksen (1997)
10.1073/PNAS.93.10.4742
The control of hematopoiesis and leukemia: from basic biology to the clinic.
L. Sachs (1996)



This paper is referenced by
The Antitumor Materials Produced by Bacillus amyloliquefaciens V 656 and Monascus purpureus BCRC 31499 Enzymes and Their
(2014)
10.1016/j.msec.2018.11.001
Thinking small, doing big: Current success and future trends in drug delivery systems for improving cancer therapy with special focus on liver cancer.
María J. Limeres (2019)
10.1016/j.phrs.2008.12.001
Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells.
H. Liu (2009)
10.1016/j.ijbiomac.2015.11.011
Chitosan oligosaccharides alleviate cognitive deficits in an amyloid-β1-42-induced rat model of Alzheimer's disease.
Shiliang Jia (2016)
10.1080/13102818.2004.10817099
Antileukemic Activity of Epirubicin Conjugated with Biopolymer Dextran Against Lymphoid Leukemia L1210 as Tumor Model
N. Todorova (2004)
10.4028/www.scientific.net/JNanoR.32.113
Chitosan Nanocarriers Loading Anti-Tumor Drugs
J. W. Wu (2015)
10.1016/j.ijbiomac.2020.06.123
Biochemical and antifungal characteristics of recombinant class I chitinase from Drosera rotundifolia.
Miroslav Rajninec (2020)
10.1208/s12249-009-9279-1
Amyloid-Beta Associated with Chitosan Nano-Carrier has Favorable Immunogenicity and Permeates the BBB
Zhang Song-jiang (2009)
10.1177/1090820X11411475
Animal model of implant capsular contracture: effects of chitosan.
M. Marques (2011)
10.1002/1522-2683(200208)23:15<2490::AID-ELPS2490>3.0.CO;2-3
Biomic study of human myeloid leukemia cells differentiation to macrophages using DNA array, proteomic, and bioinformatic analytical methods
H. Juan (2002)
10.1016/j.ijbiomac.2012.10.009
Anticancer activity of chemically prepared shrimp low molecular weight chitin evaluation with the human monocyte leukaemia cell line, THP-1.
Rym Salah (2013)
10.1007/s11274-011-0910-4
Chitooligosaccharides antagonize the cytotoxic effect of glucosamine
C. F. Assis (2012)
10.1155/2014/827847
Chitosan Oligosaccharides Attenuate Ocular Inflammation in Rats with Experimental Autoimmune Anterior Uveitis
I. Fang (2014)
10.1007/S10973-006-7684-1
Drug–polymer interaction in the all-trans retinoic acid release from chitosan microparticles
A. M. Lira (2007)
10.1016/j.ijpharm.2010.08.039
Antitumor activity of quaternized chitosan-based electrospun implants against Graffi myeloid tumor.
R. Toshkova (2010)
Optimization of a flow cytometric method to confirm the differentiation of HL-60 cell lines by candidate chemicals as indicated by screening tests
P. S. Wismayer (2017)
10.1016/J.BCP.2004.02.021
Induction of apoptosis in two human leukemia cell lines as well as differentiation in human promyelocytic cells by cyanidin-3-O-beta-glucopyranoside.
C. Fimognari (2004)
10.1080/13102818.2005.10817204
Antitumor Effect of Conjugate EX1 of Epirubicin Against Lymphocytic Leukemia L 1210
N. Todorova (2005)
10.1007/S12257-009-3135-8
Proteome analysis in adipose tissue of ob/ob mice in response to chitosan oligosaccharides treatment
Md. Atiar Rahman (2010)
10.1007/s42690-020-00171-2
Effects of chitosan nanoparticles, ivermectin and their combination in the treatment of Gasterophilus intestinalis (Diptera: Gasterophilidae) larvae in donkeys ( Equus asinus )
N. A. AbdElKader (2020)
10.1016/j.acthis.2012.03.003
Polarization optical-histochemical characterization and supramolecular structure of carbohydrate fibrils.
L. Csóka (2013)
10.1016/S0955-2863(01)00218-2
High molecular weight water-soluble chitosan protects against apoptosis induced by serum starvation in human astrocytes.
H. N. Koo (2002)
10.1081/IPH-200026887
Inhibitory Effect of Water‐Soluble Chitosan on TNF‐α and IL‐8 Secretion from HMC‐1 Cells
M. Kim (2004)
10.1201/B10120-17
Cosmeceutical Applications of Chitosan and Its Derivatives
S. Kim (2016)
10.1248/BPB.26.1326
Antioxidant activities of chitobiose and chitotriose.
A. Chen (2003)
10.1016/J.BIORTECH.2007.08.035
Reclamation of chitinous materials by bromelain for the preparation of antitumor and antifungal materials.
S. Wang (2008)
10.1016/J.BBRC.2007.03.094
N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro.
Z. Wang (2007)
10.1016/J.CARBPOL.2007.06.022
Chitooligosaccharides induce apoptosis of human hepatocellular carcinoma cells via up-regulation of Bax
Q. Xu (2008)
10.1111/j.1751-1097.2005.tb01541.x
Enhancement of Laser Cancer Treatment by a Chitosan‐derived Immunoadjuvant ¶
Wei R. Chen (2005)
10.1111/J.1745-4514.2011.00628.X
WATER-SOLUBLE CHITOSAN SENSITIZES APOPTOSIS IN HUMAN LEUKEMIA CELLS VIA THE DOWNREGULATION OF BCL-2 AND DEPHOSPHORYLATION OF AKT
Mun-Ock Kim (2013)
10.1016/J.BIOCEL.2004.11.015
Proteomic analysis of interleukin 6-induced differentiation in mouse myeloid leukemia cells.
Q. Xia (2005)
10.1248/BPB.28.1971
Effect of chitobiose and chitotriose on carbon tetrachloride-induced acute hepatotoxicity in rats.
A. Chen (2005)
See more
Semantic Scholar Logo Some data provided by SemanticScholar