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Chapter 18 Immunosensors For Clinical And Environmental Applications Based On Electropolymerized Films: Analysis Of Cholera Toxin And Hepatitis C Virus Antibodies In Water And Serum

S. Cosnier, M. Holzinger
Published 2007 · Chemistry

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Publisher Summary Because of the vast achievements in immunosensor development, only few highlights of novel immobilization techniques and investigative detection principles based on electrogenerated polypyrrole films are presented in this chapter with cholera toxin and hepatitis C Virus (HCV) as exemplary target analyte. Immobilization techniques include affinity immobilization based on biotin–avidin interactions, photoaffinity immobilization, and photochemical immobilization. Besides groundbreaking immobilization methods of antibodies, innovative detection strategies developed for high-performance immunosensors are illustrated with antibodies of cholera toxin or HCV as target. Enzymes that catalyze light-emitting processes or the formation of redox active components labeled the recognized target. Besides labeling for signal receipt, an example is highlighted where the immobilized antibody (anti-CT) is directly detected. The presented examples of recent achievements in immunosensor development show the tremendous progress in this field. Detection limits lower continuously, miniaturized setups by keeping the possibility of automated production progressed, and procedures for detection are constantly simplified. Direct immobilization of the receptor antibody by photochemical reactions enables the production of ready to use immunosensors in few steps. With photovoltaic components, time consuming and expensive labeling procedures of the analyte can be avoided.
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