Online citations, reference lists, and bibliographies.
← Back to Search

Protein Release From Gelatin Matrices.

Ikada, Tabata
Published 1998 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
Gelatin is a denatured, biodegradable protein obtained by acid and alkaline processing of collagen. This processing affects the electrical nature of collagen, yielding gelatin with different isoelectric points (IEPs). When mixed with positively or negatively charged gelatin, an oppositely charged protein will ionically interact to form a polyion complex. This review article describes protein release from charged gelatin matrices on the basis of this polyion complexation. The biodegradable hydrogel matrices are prepared by chemical crosslinking of acidic or basic gelatin and are enzymatically degraded in the body with time. The degradation is controllable by changing the extent of crosslinking, which, in turn, produces hydrogels with different water contents. The time course of protein release is in good accordance with the rate of hydrogel degradation. It is very likely that the protein drug complexed with gelatin hydrogel is released as a result of its biodegradation. This gelatin hydrogel system releases the protein drug under maintenance of biological activity. This article will focus on experimental data that sustained release of growth factor from the gelatin hydrogels is very effective in exerting the biological functions of the growth factor.
This paper references
10.1016/0168-3659(94)00035-2
Enhanced vascularization and tissue granulation by basic fibroblast growth factor impregnated in gelatin hydrogels
Y. Tabata (1994)
10.1210/EDRV-8-2-95
Structural characterization and biological functions of fibroblast growth factor.
D. Gospodarowicz (1987)
10.1111/j.1749-6632.1991.tb49012.x
Biological Activities of Fibroblast Growth Factors
D. Gospodarowicz (1991)
10.1016/0168-3659(94)90233-X
Characterization and morphological analysis of protein-loaded poly(lactide-co-glycolide) microparticles prepared by water-in-oil-in-water emulsion technique
C. Yan (1994)
10.1016/0168-3659(93)90070-L
A formulation method using D,L-lactic acid oligomer for protein release with reduced initial burst
Y. Tabata (1993)
10.1002/JBM.820240907
Macromolecular release from collagen monolithic devices.
D. Gilbert (1990)
10.1097/00000658-198812000-00019
Acceleration of tensile strength of incisions treated with EGF and TGF-beta.
G. L. Brown (1988)
10.1016/0092-8674(91)90173-V
A dual receptor system is required for basic fibroblast growth factor activity
M. Klagsbrun (1991)
10.1021/MA00027A019
Poly(L-lactic acid)/pluronic blends : characterization of phase separation behavior, degradation, and morphology and use as protein-releasing matrices
T. G. Park (1992)
10.1002/JNR.490230306
Basic fibroblast growth factor released from synthetic guidance channels facilitates peripheral nerve regeneration across long nerve gaps
P. Aebischer (1989)
10.1016/0168-3659(94)90030-2
Calcium-alginate beads for the oral delivery of transforming growth factor-β1 (TGF-β1) : stabilization of TGF-β1 by the addition of polyacrylic acid within acid-treated beads
R. Mumper (1994)
10.1016/S0264-410X(96)00098-9
Adjuvant effect of biodegradable poly(DL-lactic acid) granules capable for antigen release following intraperitoneal injection.
R. Nakaoka (1996)
10.1163/156856294X00644
Controlled release of TGF-β1 from a biodegradable matrix for bone regeneration
W. Gombotz (1994)
10.1201/9781003067542
Microparticulate systems for the delivery of proteins and vaccines
S. Cohen (1996)
10.1016/0168-3659(87)90074-5
Prolonged delivery of peptides by microcapsules
H. V. Maulding (1987)
Controlled release, biodegradable cytokine depots: a new approach in cancer vaccine design.
P. Golumbek (1993)
10.1210/ENDO.135.2.8033826
Stimulation of fracture repair by recombinant human basic fibroblast growth factor in normal and streptozotocin-diabetic rats.
H. Kawaguchi (1994)
10.1016/0304-3940(92)90684-Y
Microencapsulated nerve growth factor: Effects on the forebrain neurons following devascularizing cortical lesions
D. Maysinger (1992)
10.1096/fasebj.11.1.9034166
Growth factors in the extracellular matrix
J. Taipale (1997)
10.1016/0142-9612(91)90107-L
Controlled and modulated release of basic fibroblast growth factor.
E. Edelman (1991)
10.1016/0168-3659(90)90130-L
Albumin-heparin microspheres as carriers for cytostatic agents
H. Cremers (1990)
10.1002/JPS.2600740308
Novel method for the preparation of controlled-release theophylline granules coated with a polyelectrolyte complex of sodium polyphosphate-chitosan.
Y. Kawashima (1985)
10.1021/BC00031A002
DNA complexes with polycations for the delivery of genetic material into cells.
A. Kabanov (1995)
10.1016/0168-3659(91)90131-V
Stability of atriopeptin III in poly(d,l-lactide-co-glycolide) microspheres
R. Johnson (1991)
10.1002/JPS.2600731103
Characterization of polyacryl starch microparticles as carriers for proteins and drugs.
P. Artursson (1984)
10.1016/S0232-1513(82)80054-6
Stimulation of wound healing, using brain extract with fibroblast growth factor (FGF) activity. II. Histological and morphometric examination of cells and capillaries.
P. Buntrock (1982)
10.1016/0022-4804(88)90034-0
Recombinant basic fibroblast growth factor accelerates wound healing.
G. McGee (1988)
10.1016/0168-3659(92)90014-I
Factors influencing the release of peptides and proteins from biodegradable parenteral depot systems
D. Bodmer (1992)
10.3109/10731198809117571
Insulin controlled-release microcapsules to prolong the hypoglycemic effect in diabetic rats.
S. Lin (1988)
10.1016/0168-3659(94)00106-5
Long-acting delivery system of interferon: IFN minipellet
K. Fujioka (1995)
10.1002/JPS.2600830527
In vitro drug release behavior of D,L-lactide/glycolide copolymer (PLGA) nanospheres with nafarelin acetate prepared by a novel spontaneous emulsification solvent diffusion method.
T. Niwa (1994)
10.1016/0168-3659(94)90026-4
The influence of biodegradable microcapsule formulations on the controlled release of a protein
H. Sah (1994)
10.1042/BST0130520
Biodegradable polymers for the sustained release of peptides.
F. G. Hutchinson (1985)
10.1089/HUM.1995.6.9-1129
Nonviral gene therapy: the promise of genes as pharmaceutical products.
F. Ledley (1995)
10.1002/JPS.2600690725
Immobilization of proteins in microspheres of biodegradable polyacryldextran.
P. Edman (1980)
Growth factor actions on articular cartilage.
S. Trippel (1995)
10.1016/0168-3659(92)90178-T
Controlled release from poly ( vinyl alcohol ) gels prepared by freezing-thawing processes
N. Peppas (1992)
10.1002/JCP.1041520225
Calcium alginate beads as a slow‐release system for delivering angiogenic molecules In Vivo and In Vitro
E. C. Downs (1992)
10.1210/ENDO.136.3.7867582
Stimulation of endosteal bone formation by systemic injections of recombinant basic fibroblast growth factor in rats.
T. Nakamura (1995)
10.3171/JNS.1997.86.5.0871
Potential efficacy of basic fibroblast growth factor incorporated in biodegradable hydrogels for skull bone regeneration.
K. Yamada (1997)
The Macromolecular Chemistry of Gelatin
A. Veis (1964)
10.1163/156856298X00569
Complexation of basic fibroblast growth factor with gelatin.
Muniruzzaman (1998)
10.1016/0168-3659(92)90140-M
Biodegradable nanoparticles for subcutaneous administration of growth hormone releasing factor (hGRF)
J. Gautier (1992)
10.1163/156856297X00254
Lysozyme loading and release from hydrogels carrying pendant phosphate groups.
K. Nakamae (1997)
10.1016/0168-3659(95)00166-2
Controllable gene therapy pharmaceutics of non-viral gene delivery systems
E. Tomlinson (1996)
10.1073/PNAS.90.2.552
Erosion kinetics of hydrolytically degradable polymers.
J. Tamada (1993)
10.1016/0168-3659(93)90095-M
Controlled release of albumin from biodegradable poly(DL-lactide) cylinders
X. Zhang (1993)
10.1016/S0006-291X(88)80887-8
Basic fibroblast growth factor (FGF) promotes cartilage repair in vivo.
P. Cuevas (1988)
10.1016/0168-3659(94)90082-5
Biodegradable microspheres as depot system for patenteral delivery of peptide drugs
R. Mehta (1994)
10.1016/0168-3659(94)00083-7
Development of an in situ forming biodegradable poly-lactide-coglycolide system for the controlled release of proteins
W. J. Lambert (1995)
10.3233/BME-1992-2304
Flexibilized gelatin film-based artificial skin model: II. Release kinetics of incorporated bioactive molecules.
B. G. Shinde (1992)
10.1016/0142-9612(83)90025-X
Controlled release of water-soluble macromolecules from bioerodible hydrogels.
J. Heller (1983)
10.1002/JPS.2600790509
Kinetics of protein diffusion from a poly(D,L-lactide) reservoir system.
N. Marcotte (1990)
10.1073/PNAS.90.4.1513
Perivascular and intravenous administration of basic fibroblast growth factor: vascular and solid organ deposition.
E. Edelman (1993)
10.1016/0378-5173(92)90223-O
Hyaluronane derivative microspheres as NGF delivery devices: Preparation methods and in vitro release characterization
E. Ghezzo (1992)
10.1016/0168-3659(93)90165-2
Controlled growth factor delivery induces differential neurite outgrowth in three-dimensional cell cultures
Christine E. Beaty (1993)
10.1016/0168-3659(92)90076-4
Blends of PVA and PGLA: control of the permeability and degradability of hydrogels by blending
G. Pitt (1992)
Protein release from poly(lactic-co-glycolic acid) microspheres: protein stability problems.
W. Lu (1995)
10.1111/j.2042-7158.1994.tb03820.x
Effects of epidermal growth factor dosage forms on dermal wound strength in mice
N. Çelebi (1994)
10.1002/JPS.2600760613
Binding mechanism of doxorubicin in ion-exchange albumin microcapsules.
A. Sawaya (1987)
10.1023/A:1018942118148
Determinants of Release Rate of Tetanus Vaccine from Polyester Microspheres
M. Alonso (2004)
10.1002/MICR.1920150312
Effect of basic fibroblast growth factor and α‐melanocytic stimulating hormone on nerve regeneration through a collagen channel
A. Laquérriere (1994)
10.1038/NBT0890-755
Controlled Release of Interleukin-2 from Biodegradable Microspheres
M. Hora (1990)
10.1097/00003086-199309000-00050
Polylactic acid-polyethylene glycol block copolymer. A new biodegradable synthetic carrier for bone morphogenetic protein.
S. Miyamoto (1993)
10.1016/0168-3659(93)90041-3
Preformed porous microspheres for controlled and pulsed release of macromolecules
A. Supersaxo (1993)
10.1002/MARC.1985.030060710
Biodegradability of a hot-pressed poly(lactic acid) formulation with controlled release of LH-RH agonist and its pharmacological influence on rat prostate
Maszharu Asano (1985)
10.1016/0168-3659(92)90193-U
Release of proteins via ion exchange from albumin-heparin microspheres
G. Kwon (1992)
10.1227/00006123-199203000-00001
Sustained release of nerve growth factor from biodegradable polymer microspheres.
P. J. Camarata (1992)
10.1016/0168-3659(86)90033-7
In vitro and in vivo release of insulin from poly(lactic acid) microbeads and pellets
A. K. Kwong (1986)
10.1016/0168-3659(94)90129-5
Hyaluronic acid ester microspheres as a nasal delivery system for insulin
L. Illum (1994)
10.3109/08977199109000293
TGF-β1 Accelerates Wound Healing: Reversal of Steroid-Impaired Healing in Rats and Rabbits
L. S. Beck (1991)
10.1201/9781003067542-4
POLYPHOSPHAZENE HYDROGEL MICROSPHERES FOR PROTEIN DELIVERY
A. K. Andrianov (1996)
10.1016/0168-3659(94)00126-F
Fibrin-based drug delivery systems III: The evaluation of the release of macromolecules from microbeads
H. Ho (1995)
10.1016/0168-3659(94)90050-7
In-vitro degradation and bovine serum albumin release of the ABA triblock copolymers consisting of poly (L(+) lactic acid), or poly(L(+) lactic acid-co-glycolic acid) A-blocks attached to central polyoxyethylene B-blocks
L. You-xin (1994)
10.3109/03008208909023898
Effects of acidic and basic fibroblast growth factors on osteoblastic cells.
S. Rodan (1989)
10.1016/0168-3659(94)00060-8
Polyisobutylcyanoacrylate nanoparticles as sustained release system for calcitonin
C. Tasset (1994)
10.1016/0304-3940(92)90885-B
Protective effect of NGF atelocollagen mini-pellet on the hippocampal delayed neuronal death in gerbils
S. Yamamoto (1992)



This paper is referenced by
10.1016/j.biomaterials.2010.09.054
The effect of biodegradable gelatin microspheres on the neuroprotective effects of high mobility group box 1 A box in the postischemic brain.
Yin-Chuan Jin (2011)
10.1163/156856203322553437
Reagent-free crosslinking of aqueous gelatin: manufacture and characteristics of gelatin gels irradiated with gamma-ray and electron beam
K. Terao (2003)
10.1155/2019/9423565
Auto-Cross-Linking Hydrogels of Hydrogen Peroxide-Oxidized Pectin and Gelatin for Applications in Controlled Drug Delivery
C. Garrido (2019)
10.1155/2012/378356
Neural Stem Cells in the Adult Brain: From Benchside to Clinic
O. Gonzalez-Perez (2012)
10.1007/978-1-60327-905-5_19
Tissue Engineered Scaffolds for Stem Cells and Regenerative Medicine
H. Hosseinkhani (2009)
10.1002/mabi.200700334
Recruitment of endogenous stem cells for tissue repair.
Jing Zhao (2008)
10.11392/JSAO1972.36.108
ドラッグデリバリーシステム (DDS) と組織工学
泰彦 田畑 (2007)
10.1016/J.SURG.2007.03.016
Controlled release of basic fibroblast growth factor promotes healing of the pancreaticojejunal anastomosis: a novel approach toward zero pancreatic fistula.
T. Aimoto (2007)
10.1097/MAT.0b013e31815fd3d3
Tracheal Cartilage Regeneration and New Bone Formation by Slow Release of Bone Morphogenetic Protein (BMP)-2
H. Igai (2008)
10.3390/ijms10083442
The Role of Bloom Index of Gelatin on the Interaction with Retinal Pigment Epithelial Cells
J. Lai (2009)
10.26717/BJSTR.2018.04.001025
Periodontal Regeneration Using Gelatin Hydrogels Incorporating Basic Fibroblast Growth Factor
Goichi Matsumoto (2018)
10.3389/fphar.2018.01329
Targeted Delivery of Bioactive Molecules for Vascular Intervention and Tissue Engineering
Hannah A. Strobel (2018)
10.1007/978-3-319-25322-0_4
Protein-Based Hydrogels
Alexandra L. Rutz (2016)
10.1016/B978-1-78242-078-1.00011-6
Surface modification by natural biopolymer coatings on magnesium alloys for biomedical applications
Z. Zhen (2015)
10.1089/TEN.TEB.2016.0280
Design and Use of Chimeric Proteins Containing a Collagen-Binding Domain for Wound Healing and Bone Regeneration.
AddiCyril (2016)
10.1021/la202252m
Nanofibers resulting from cooperative electrostatic and hydrophobic interactions between peptides and polyelectrolytes of opposite charge.
M. Ferstl (2011)
Authors for correspondence
A. Stace (2017)
10.1021/bm801103c
Growth factor delivery approaches in hydrogels.
A. Silva (2009)
10.1016/j.ijpharm.2008.04.030
Bioinspired tissue engineering: the great promise of protein delivery technologies.
F. Quaglia (2008)
10.1089/ten.tea.2007.0346
Delivery of basic fibroblast growth factor from gelatin microsphere scaffold for the growth of human umbilical vein endothelial cells.
X. H. Zhu (2008)
10.1002/jbm.b.32806
Platelet-rich plasma-loaded chitosan scaffolds: preparation and growth factor release kinetics.
Burak Kutlu (2013)
10.4030/JJCS.34.1019
A Patient who Underwent Reconstruction of Nasal–Alar with Auricular Helix Composite Graft and Application of bFGF
Kazuhide Matsunaga (2009)
10.1016/J.MSEC.2008.10.036
Fabrication and characterization of a novel microparticle with gyrus-patterned surface and growth factor delivery for cartilage tissue engineering
Sha Huang (2009)
10.1089/107632702760240526
Time course of de novo adipogenesis in matrigel by gelatin microspheres incorporating basic fibroblast growth factor.
Y. Kimura (2002)
10.1016/j.jconrel.2009.10.018
Naturally derived materials-based cell and drug delivery systems in skin regeneration.
S. Huang (2010)
10.1021/acsami.6b01364
Gelatin Microgel Incorporated Poly(ethylene glycol)-Based Bioadhesive with Enhanced Adhesive Property and Bioactivity
Yuting Li (2016)
10.1039/C1SM06261A
A pH-responsive coiled-coil peptide hydrogel
N. Fletcher (2011)
10.1016/J.ACVSP.2008.06.015
Inhibición experimental del crecimiento de aneurismas aórticos abdominales mediante esponja biodegradable con hidrogel de gelatina
N. Miyama (2009)
10.1163/156856209X449452
Cross-Linking and Depolymerisation of γ-Irradiated Fish Gelatin and Porcine Gelatin Studied by SEC-MALLS and SDS-PAGE: A Comparative Study
M. Hara (2010)
10.1007/s11696-020-01234-x
6-acylamino nicotinic acid-based hydrogelators applicable in phase selective gelation, reproducible mat formation and toxic dye removal
Siddhartha Das (2020)
10.1016/B978-0-08-055294-1.00238-5
Biomaterials for Spinal Cord Repair
Melissa G. D. Baumann (2011)
10.1016/J.BIOMATERIALS.2006.12.006
Modulation of protein delivery from modular polymer scaffolds.
M. Lee (2007)
See more
Semantic Scholar Logo Some data provided by SemanticScholar