← Back to Search
Polyethylene Glycol-coated Liposomes For Oral Delivery Of Recombinant Human Epidermal Growth Factor.
H. Li, Jong Hwa Song, J. Park, K. Han
Published 2003 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
The present study was to investigate the feasibility of oral delivery of recombinant human epidermal growth factor (rhEGF). Polyethylene glycol (PEG)-coated liposomes containing rhEGF was prepared and evaluated for their stability and permeability in Caco-2 cells. In the animal study, we also determined plasma concentration and gastric ulcer healing effect after oral administration of rhEGF liposomes or the solution. Encapsulation of rhEGF into liposomes, suppressed the degradation in Caco-2 cell homogenate compared with the solution. The flux of rhEGF from dipalmitoylphosphatidylcholine (DPPC) liposome across Caco-2 cell monolayer from the apical to basolateral side was three times greater than that from phosphatidylcholine (PC) liposome or the solution. After oral administration of rhEGF liposomes or the solution in rats, the area under the concentration-time curve (AUC) of rhEGF increased 1.7- and 2.5-fold for PC and DPPC liposomes, respectively. The gastric ulcer healing effect was significantly increased in DPPC liposome compared with PC liposome and the solution. The enhanced curative ratio of rhEGF encapsulated into DPPC liposome may be due to the resistance to enzyme degradation, higher permeability and increased plasma AUC. Therefore, PEG-coated liposomes containing rhEGF could be used as an oral delivery formulation with enhanced encapsulation efficiency.
This paper references
Site-specific degradation and transport of recombinant human epidermal growth factor (rhEGF) in the rat gastrointestinal mucosa
Kun Han (1998)
Gastric cytoprotection by epidermal growth factor. Role of endogenous prostaglandins and DNA synthesis.
S. Konturek (1981)
Metabolism of a neurotensin (8–13) analog by intestinal and nasal enzymes, and approaches to stabilize this peptide at these absorption sites
B. Aungst (1995)
Mucoadhesive nanoparticulate systems for peptide drug delivery.
H. Takeuchi (2001)
The inhibition of gastric acid secretion by epidermal growth factor
J. Bower (2005)
Role of mucosal prostaglandins and DNA synthesis in gastric cytoprotection by luminal epidermal growth factor.
S. Konturek (1981)
Site-dependent effect of aprotinin, sodium caprate, Na2EDTA and sodium glycocholate on intestinal absorption of insulin.
M. Morishita (1993)
Preparation and Use of Liposomes as Models of Biological Membranes
A. D. Bangham (1974)
Transport, Uptake, and Metabolism of the Bis(pivaloyloxymethyl)-Ester Prodrug of 9-(2- Phosphonylmethoxyethyl)Adenine in an In Vitro Cell Culture System of the Intestinal Mucosa (Caco-2)
P. Annaert (2004)
Gastrointestinal Uptake of Biodegradable Microparticles: Effect of Particle Size
M. P. Desai (2004)
Oral delivery of insulin by using surface coating liposomes improvement of stability of insulin in GI tract
K. Iwanaga (1997)
Pharmacodynamics of insulin in polyethylene glycol-coated liposomes.
A. Kim (1999)
Epidermal growth factor inhibits cysteamine-induced duodenal ulcers.
P. Kirkegaard (1983)
Aqueous Stability of Human Epidermal Growth Factor 1-48
R. I. Senderoff (2004)
Application of surface-coated liposomes for oral delivery of peptide: effects of coating the liposome's surface on the GI transit of insulin.
K. Iwanaga (1999)
Microparticulate absorption from the rat intestine
P. G. Jenkins (1994)
Enhanced Encapsulation of Amphotericin B into Liposomes by Complex Formation with Polyethylene Glycol Derivatives
K. Moribe (2004)
Folic acid-PEO-labeled liposomes to improve gastrointestinal absorption of encapsulated agents.
K. E. Anderson (1999)
Encapsulation characteristics of nystatin in liposomes: effects of cholesterol and polyethylene glycol derivatives.
K. Moribe (1999)
Isolation and structure of urogastrone and its relationship to epidermal growth factor
H. Gregory (1975)
Drug delivery studies in Caco-2 monolayers. II. Absorption enhancer effects of lysophosphatidylcholines
L. Hovgaard (1995)
Demonstration of Sucralfate-mediated Preservation of Growth Factor Bioactivity in the Presence of Low pH with a Human Gastric Epithelial Cell Line (AGS)
H. Sheng (2004)
Effect of urogastrone on gastric secretion and plasma gastrin levels in normal subjects.
J. Elder (1975)
Formulation and Evaluation of a Folic Acid Receptor-Targeted Oral Vancomycin Liposomal Dosage Form
K. E. Anderson (2004)
Epidermal growth factor (EGF).
R. Goodlad (1996)
This paper is referenced by
The evaluation of stability of recombinant human epidermal growth factor in burn-injured pigs
C. Yang (2005)
Ultrasound Activated Bone Morphogenetic -2 (BMP-2) Nanocomplexes that Provides On-Demand, Controlled Release for Bone Regeneration
Gazelle J Crasto (2016)
Application 6 – Design of nanoparticles for oral delivery of peptide drugs
Hideki Ichikawa (2012)
Separation of polyethylene glycols and their amino-substituted derivatives by high-performance gel filtration chromatography at low ionic strength with refractive index detection.
M. Liu (2004)
Liposomes‐in‐Chitosan Hydrogels: Challenges and Opportunities for Biomedical Applications
S. Grijalvo (2019)
Oral Delivery of Macromolecules for the Development of Therapeutic Agents
H. Lee (2011)
Oral delivery of liposomes.
W. Wu (2015)
Recent advances with liposomes as pharmaceutical carriers
V. Torchilin (2005)
Bolalipid-Doped Liposomes: Can Bolalipids Increase the Integrity of Liposomes Exposed to Gastrointestinal Fluids?
S. Müller (2019)
Preparation of redispersible liposomal dry powder using an ultrasonic spray freeze-drying technique for transdermal delivery of human epithelial growth factor
F. Yin (2014)
Tamoxifen/raloxifene loaded liposomes for oral treatment of breast cancer
N. B. M. Ağardan (2020)
Investigation of epidermal growth factor containing liposome formulation effects on burn wound healing.
Ceren Alemdaroğlu (2008)
Doxorubicin-loaded liposomes: enhancing the oral bioavailability by modulation of physicochemical characteristics.
Marjan Daeihamed (2017)
Exposure assessment of epidermal growth factor to various tissues in mice after intravenous and subcutaneous administration
J. B. Lee (2015)
rhEGF microsphere formulation and in vitro skin evaluation
L. A. Haushey (2010)
Oral delivery of anticancer drugs III: formulation using drug delivery systems.
Silvia Mazzaferro (2013)
Investigation of archaeosomes as carriers for oral delivery of peptides.
Z. Li (2010)
Elastic liposomes as carriers for oral delivery and the brain distribution of (+)-catechin
Yaw-Bin Huang (2011)
Camptothecin-Loaded Liposomes with α-Melanocyte-Stimulating Hormone Enhance Cytotoxicity Toward and Cellular Uptake by Melanomas: An Application of Nanomedicine on Natural Product
C. Lin (2013)
Liposomes coated with N-trimethyl chitosan to improve the absorption of harmine in vivo and in vitro
W. Chen (2016)
Evaluation of the release rate of bioactive recombinant human epidermal growth factor from crosslinking collagen sponges
C. Yang (2008)
Relationship between N,N-dialkyl chitosan monolayer and corresponding vesicle.
M. Li (2007)
The Effectiveness of Raloxifene-Loaded Liposomes and Cochleates in Breast Cancer Therapy
N. B. M. Ağardan (2015)
Nanotechnology to Improve Oral Drug Delivery
M. Bhavsar (2008)
A lipid based multi-compartmental system: Liposomes-in-double emulsion for oral vaccine delivery.
J. J. Liau (2015)
Mixing behaviour of asymmetrical glycerol diether bolalipids with saturated and unsaturated phosphatidylcholines.
S. Müller (2018)
Oral delivery of anticancer drugs: challenges and opportunities.
K. Thanki (2013)
Characterization and Modification of Nanodiamond-Doxorubicin Complexes for Treatment of Colorectal Cancer
Bryan Lee (2015)
Multivesicular liposomes for oral delivery of recombinant human epidermal growth factor
H. Li (2005)
Carboxymethyl-chitosan-tethered lipid vesicles: hybrid nanoblanket for oral delivery of paclitaxel.
Nitin Joshi (2013)See more