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Reaction Of Peroxidase With Reduced Nicotinamide-adenine Dinucleotide And Reduced Nicotinamide-adenine Dinucleotide Phosphate.
K. Yokota, I. Yamazaki
Published 1965 · Chemistry, Medicine
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Summary 1. Peroxidase (donor:H2O2 oxidoreductase, EC 220.127.116.11) catalyzes the aerobic oxidation of NADH and NADPH at acidic pH in the absence of cofactors such as Mn2+ and monophenols. 2. Peroxidase Compound III appears during the reaction and disappears when almost all the oxygen in the reaction solution has been consumed. 3. A part of peroxidase is rapidly reduced with consumption of H2O2 or O2 in the presence of NADH and NADPH. 4. It is suggested that the free radicals derived from NADH and NADPH are active intermediates in the formation of Compound III and the reduction of peroxidase. 5. The different types of peroxidase-oxidase reaction possible when dihydroxy-fumarate, triose reductone, indole acetate and NADH are used as hydrogen donors are discussed.
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