Online citations, reference lists, and bibliographies.
Referencing for people who value simplicity, privacy, and speed.
Get Citationsy
← Back to Search

[Cell Sheet Engineering].

T. Okano
Published 2006 · Medicine, Materials Science

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Cell sheets can be prepared by culturing cells at 37 degrees C on temperature-responsive surfaces, modified with poly (N-isopropylacrylamide) at a thickness of 20-30 nm. Cultured cell sheets are successfully harvested and manipulated by simple temperature changes from 37 degrees C to 20 degrees C due to hydrophobic/hydrophilic changes of the dish surfaces. This technology has led to "cell sheet engineering" which has initiated new therapies for tissue engineering and regenerative medicine. Various cell sheets such as corneal epithelium, corneal endothelium, skin, periodontal ligaments, oral mucosal epithelium, urothelium, and cardiomyocyte tissues have already been fabricating using these temperature-responsive cell culture surfaces. Additionally, we have already initiated clinical treatments using both corneal and oral mucosal epithelial cell sheets for the treatment of patients with corneal surface diseases. By creating layered cell sheets, structural and functional connections can be achieved. Therefore, we have successfully prepared synchronously beating heart tissues in vitro. Finally, we are also examining the use of heterogeneously layered cell sheets to create laminar structures that resemble the functional and structural units of complex organs such as the liver and kidney.
This paper references
10.1097/01.TP.0000110320.45678.30
Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface
K. Nishida (2004)
Tissue engineering : Frontiers in biotechnology
R. Langer (1993)
10.1161/HH0302.105722
Fabrication of Pulsatile Cardiac Tissue Grafts Using a Novel 3-Dimensional Cell Sheet Manipulation Technique and Temperature-Responsive Cell Culture Surfaces
T. Shimizu (2002)
10.1002/JBM.820271005
A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide).
T. Okano (1993)
10.1089/10763270152436517
Thermo-responsive culture dishes allow the intact harvest of multilayered keratinocyte sheets without dispase by reducing temperature.
M. Yamato (2001)
10.1089/107632703322495646
Transplantable urothelial cell sheets harvested noninvasively from temperature-responsive culture surfaces by reducing temperature.
Y. Shiroyanagi (2003)
10.1002/(SICI)1097-4636(19990615)45:4<355::AID-JBM10>3.0.CO;2-7
Decrease in culture temperature releases monolayer endothelial cell sheets together with deposited fibronectin matrix from temperature-responsive culture surfaces.
A. Kushida (1999)
10.1016/S0142-9612(00)00270-2
Intact microglia are cultured and non-invasively harvested without pathological activation using a novel cultured cell recovery method.
K. Nakajima (2001)
10.1097/00006534-199708000-00001
Transplantation of Chondrocytes Utilizing a Polymer‐Cell Construct to Produce Tissue‐Engineered Cartilage in the Shape of a Human Ear
Y. Cao (1997)
10.1002/JBM.1284
Electrically communicating three-dimensional cardiac tissue mimic fabricated by layered cultured cardiomyocyte sheets.
T. Shimizu (2002)
10.1016/S0142-9612(03)00110-8
Cell sheet engineering for myocardial tissue reconstruction.
T. Shimizu (2003)
10.1097/00003226-200310001-00005
Tissue Engineering of the Cornea
K. Nishida (2003)
10.1002/MARC.1990.030111109
Thermo‐responsive polymeric surfaces; control of attachment and detachment of cultured cells
Noriko Yamada (1990)
10.1021/BM0002961
Creation of designed shape cell sheets that are noninvasively harvested and moved onto another surface.
M. Hirose (2000)



This paper is referenced by
10.1016/j.biomaterials.2013.09.104
Expression profiles of angiogenesis-related proteins in prevascular three-dimensional tissues using cell-sheet engineering.
Tadashi Sasagawa (2014)
10.3389/fbioe.2020.00816
Modular Orthopaedic Tissue Engineering With Implantable Microcarriers and Canine Adipose-Derived Mesenchymal Stromal Cells
Chara Simitzi (2020)
10.1002/jbm.a.36014
Fabrication and characterization of cell sheets using methylcellulose and PNIPAAm thermoresponsive polymers: A comparison Study.
Anoosha Forghani (2017)
Effect of Micro Ridges on Orientation of Cultured Cell
Haruka Hino (2014)
10.1016/J.MATCHEMPHYS.2011.04.058
Ultraviolet photodetectors fabricated from ZnO p-i-n homojunction structures
F. Sun (2011)
10.1016/j.actbio.2019.02.001
Thin peptide hydrogel membranes suitable as scaffolds for engineering layered biostructures.
Wei Yang Seow (2019)
10.3390/mi11050534
High-Speed Manipulation of Microobjects Using an Automated Two-Fingered Microhand for 3D Microassembly
E. Kim (2020)
10.3389/fendo.2017.00103
Functional Thyroid Follicular Cells Differentiation from Human-Induced Pluripotent Stem Cells in Suspension Culture
Ayumi Arauchi (2017)
The Fabrication of Vascular Tissue, 3D Printing and Additive Manufacturing
H. Hamdaoui (2019)
10.1039/B822177D
Extracellular matrix and tissue engineering applications
H. Fernandes (2009)
Synthesis and Properties of Thermo-Sensitive Hydrogels Based on PVA/Chitosan/PNIPAAm
E. Biazar (2011)
10.1007/12_2014_287
Soft Nanohybrid Materials Consisting of Polymer–Clay Networks
K. Haraguchi (2014)
10.2478/PJVS-2013-0119
Chitosan -- a promising biomaterial in veterinary medicine.
O. Drewnowska (2013)
10.1016/j.chroma.2011.04.015
Temperature-responsive chromatography for the separation of biomolecules.
H. Kanazawa (2011)
10.1016/J.COBME.2017.05.008
Engineering the vasculature with additive manufacturing
C. Vyas (2017)
Award Number: W81XWH-06-1-0271 TITLE: Development of a Smart Diagnostics Platform for Early-Stage Screening of Breast Cancer
J. Lahann (2008)
10.1002/term.2932
Oxygen and nutrient delivery in tissue engineering: Approaches to graft vascularization
T. Rademakers (2019)
10.1002/9783527633739.CH6
Switchable Surface Approaches
A. Ross (2011)
10.1002/9783527610419.NTLS0179
Polymer Thin Films for Biomedical Applications
V. K. Vendra (2011)
10.1080/15583720600945394
From Advanced Biomedical Coatings to Multi‐Functionalized Biomaterials
M. Yoshida (2006)
10.1016/j.actbio.2020.10.005
Complex-shaped magnetic 3D cell-based structures for tissue engineering.
L. F. Santos (2020)
10.1109/EMBC.2013.6611161
Construction of a chondrocyte cell sheet using temperature-responsive poly(N-isopropylacrylamide)-co-acrylamide
Kwanchanok Viravaidya-Pasuwat (2013)
10.3109/03008207.2016.1173035
Stem cell-derived cell-sheets for connective tissue engineering
P. Y. Neo (2016)
10.1002/jbm.b.34271
Thermo-modulated Hela cell release from an elastic and biocompatible hydrogel.
Yujing You (2018)
Biomaterials for corneal repair and regeneration
A. Patil (2015)
10.1016/j.biomaterials.2012.11.046
Engineered scaffold-free tendon tissue produced by tendon-derived stem cells.
M. Ni (2013)
10.1016/j.jcis.2009.10.004
Temperature-responsive polymer-brush constructed on a glass substrate by atom transfer radical polymerization.
H. Kitano (2010)
10.1007/s10616-019-00325-2
Construction of cardiomyoblast sheets for cardiac tissue repair: comparison of three different approaches
Gökçe Kaynak Bayrak (2019)
10.1016/j.colsurfb.2013.01.029
Modulation of cell adhesion and detachment on thermo-responsive polymeric surfaces through the observation of surface dynamics.
Y. Kumashiro (2013)
10.3389/fphar.2021.603016
New Modalities of 3D Pluripotent Stem Cell-Based Assays in Cardiovascular Toxicity
B. Orsolits (2021)
10.1016/j.biomaterials.2012.06.077
Shear stress-dependent cell detachment from temperature-responsive cell culture surfaces in a microfluidic device.
Zhonglan Tang (2012)
10.1109/AIM.2017.8014141
Development of a simple and low-cost automated cell sheet harvesting system for tissue engineering
Teema Leangarun (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar