Online citations, reference lists, and bibliographies.

Raf-1 Activation In Gastrointestinal Carcinoid Cells Decreases Tumor Cell Adhesion.

D. Y. Greenblatt, M. Kunnimalaiyaan, H. Chen
Published 2007 · Medicine

Cite This
Download PDF
Analyze on Scholarcy
BACKGROUND Gastrointestinal carcinoid tumors are highly metastatic. Activation of the Raf-1 signaling pathway in carcinoid cells results in morphologic changes. These Raf-1-induced structural changes may affect cellular adhesion, thereby altering metastatic potential. METHODS An estrogen-inducible Raf-1 cell line (BON-raf) was used to study the effects of Raf-1 on cellular adhesion. Cell adhesion was measured before and after Raf-1 induction. Western blot analysis was used to confirm Raf-1 activation and measure levels of an essential adhesion regulator, beta-catenin. RESULTS Estrogen treatment of BON-raf cells resulted in Raf-1 activation and a marked decrease (68%) in cell adhesion. In the absence of Raf-1 induction, carcinoid cells expressed high levels of beta-catenin. Raf-1 activation led to decreased expression of beta-catenin. CONCLUSIONS Raf-1 induction in carcinoid cells results in a significant decrease in adhesion. Furthermore, the important adhesion regulator, beta-catenin, is decreased in activated BON-raf cells. These Raf-1-related changes in adhesion may alter the metastatic phenotype of carcinoid cells.
This paper references
Medullary thyroid cancer: the functions of raf-1 and human achaete-scute homologue-1.
H. Chen (2005)
Gastrointestinal Carcinoid Tumors: Factors that Predict Outcome
J. J. Gompel (2003)
Activation of the ras/raf-1 signal transduction pathway in carcinoid tumor cells results in morphologic transdifferentiation.
R. Sippel (2002)
The Raf-1 pathway: a molecular target for treatment of select neuroendocrine tumors?
M. Kunnimalaiyaan (2006)
ZM336372, a Raf-1 activator, inhibits growth of pheochromocytoma cells.
A. Kappes (2006)
Wnt signaling and cancer.
P. Polakis (2000)
Conservation of the Notch1 signaling pathway in gastrointestinal carcinoid cells.
M. Kunnimalaiyaan (2005)
Hairy Enhancer of Split-1 (HES-1), a Notch1 effector, inhibits the growth of carcinoid tumor cells.
M. Kunnimalaiyaan (2005)
Functional interaction of β-catenin with the transcription factor LEF-1
J. Behrens (1996)
A causal role for E-cadherin in the transition from adenoma to carcinoma
A. Perl (1998)
Linking Colorectal Cancer to Wnt Signaling
M. Bienz (2000)
Dissecting tumor cell invasion: epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion
J. Behrens (1989)
Treatment of advanced carcinoid tumors
A. Lal (2006)
Mutations of the human E‐cadherin (CDH1) gene
G. Berx (1998)
Raf-1 activation suppresses neuroendocrine marker and hormone levels in human gastrointestinal carcinoid cells.
R. Sippel (2003)
A mouse model of carcinoid syndrome and heart disease.
Sandeepa Musunuru (2005)
Increased Src activity disrupts cadherin/catenin-mediated homotypic adhesion in human colon cancer and transformed rodent cells.
R. Irby (2002)
Raf-induced proliferation or cell cycle arrest is determined by the level of Raf activity with arrest mediated by p21Cip1.
D. Woods (1997)
ZM336372, a Raf-1 activator, suppresses growth and neuroendocrine hormone levels in carcinoid tumor cells
J. V. Van Gompel (2005)
Regulation of neuroendocrine differentiation in gastrointestinal carcinoid tumor cells by notch signaling.
E. Nakakura (2005)
Resection of liver metastases from a noncolorectal primary: indications and results based on 147 monocentric patients.
D. Elias (1998)
The role of human achaete-scute homolog-1 in medullary thyroid cancer cells.
R. Sippel (2003)
Differentiation of medullary thyroid cancer by C-Raf-1 silences expression of the neural transcription factor human achaete-scute homolog-1.
H. Chen (1996)
Isolated liver metastases from neuroendocrine tumors: does resection prolong survival?
H. Chen (1998)
Mutations of critical amino acids affect the biological and biochemical properties of oncogenic A-Raf and Raf-1
Elizabeth Bosch (1997)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar