Synthesis Of Antimicrobial Monophase Silver-doped Hydroxyapatite Nanopowders For Bone Tissue Engineering
Vojislav Stanić, Djordje T. Janaćković, Suzana Dimitrijević, Sladjana B. Tanasković, Miodrag Mitrić, Mirjana S. Pavlović, Aleksandra Drago Krstic, Dragoljub Jovanović, Slavica Raičević
Published 2011 · Materials Science
Download PDFAnalyze on Scholarcy
Abstract Monophase silver-doped hydroxyapatite (AgxCa10−x(PO4)6(OH)2; 0.002 ≤ x ≤ 0.04) nanoparticles were prepared using a neutralization method and investigated with respect to potential medical applications. This method consists of dissolving Ag2O in solution of H3PO4, and the slow addition to suspension of Ca(OH)2 was applied for the purpose of homogenous distribution of silver ions. Characterization studies from XRD, TEM and FTIR spectra showed that obtained crystals are monophase hydroxyapatites and that particles of all samples are of nano size, with average length of 70 nm and about 15–25 nm in diameter. Antimicrobial studies have demonstrated that all silver-doped hydroxyapatite samples exhibit excellent antimicrobial activity in vitro against the following pathogens: Staphylococcus aureus, Escherichia coli and Candida albicans. The hydroxyapatite sample with the highest content of silver has shown the highest antimicrobial activity; killed all cells of E. coli and brought to more than 99% reduction in viable counts of S. aureus and C. albicans. The atomic force microscopic studies illustrate that silver-doped hydroxyapatite sample causes considerable morphological changes of microorganism cells which might be the cause of cells’ death. Hemolysis ratios of the silver-doped hydroxyapatite samples were below 3%, indicating good blood compatibility and that are promising as biomaterials.
This paper references
Staphylococci and implant surfaces: a review.
Llinos G. Harris (2006)
In vitro and in vivo biocompatibility of graded hydroxyapatite–zirconia composite bioceramic
Renfu Quan (2008)
Antimicrobial effects of silver nanoparticles.
Jun Sung Kim (2007)
Ag-Substituted Hydroxyapatite Coatings with Both Antimicrobial Effects and Biocompatibility
Qing Ling Feng (1999)
Densification behaviour and mechanisms of synthetic hydroxyapatites
E. Landi (2000)
A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus.
Qing-ling Feng (2000)
Microbial findings at failing implants.
Asa Leonhardt (1999)
Antifungal susceptibility of Candida albicans biofilms on titanium discs with different surface roughness
C S Peter Tsang (2007)
Hydroxyapatite-coated implants: a case for their use.
Harold F. Morris (1998)
Hydroxyapatite Cement in Craniofacial Reconstruction
Daniel J Verret (2005)
Antibacterial and osteogenic properties of silver-containing hydroxyapatite coatings produced using a sol gel process.
Weitian Chen (2007)
Calcium Hydroxyapatite for Medical Applications
Nikolay Zakharov (2004)
Atomic force microscopy investigation of the characteristic effects of silver ions on Escherichia coli and Staphylococcus epidermidis.
Xiaohai Yang (2010)
Properties of hydroxyapatite crystallized from high temperature alkaline solutions
B. Lazic (1996)
Efficient disinfection of Escherichia coli in water by silver loaded alumina
Qingyun Chang (2008)
Infection following the use of porous hydroxyapatite ceramic as a bone defect filler in articular fractures
Ph. Hardy (2004)
Antibacterial coatings of fluoridated hydroxyapatite for percutaneous implants.
Xiang Ge (2010)
The Nernst Equation: Determination of Equilibrium Constants for Complex Ions of Silver
Martin L. Thompson (1999)
Anti-bacterial and cytotoxic properties of plasma sprayed silver-containing HA coatings
Y G Chen (2008)
Influence of synthesis and sintering parameters on the characteristics of carbonate apatite.
E. Landi (2004)
Antimicrobial Effects of Ag Doped Hydroxyapatite Synthesized from Co-Precipitation Route
Kyung Sik Oh (2004)
The significance of infection related to orthopedic devices and issues of antibiotic resistance.
Davide Campoccia (2006)
Antibacterial effects of Ag-HAp thin films on alumina substrates
Q. L. Feng (1998)
Characterization of enhanced antibacterial effects of novel silver nanoparticles
S. Shrivastava (2007)
Antibacterial coating systems.
Andrea Montali (2006)
Toxicity screening of liposomes.
Michael J. Parnham (1993)
The effect of hydroxyapatite crystallinity on hemolysis
John H. Wiessner (2007)
Argyria secondary to ingestion of homemade silver solution.
Douglas Brandt (2005)
In vivo evaluation of plasma sprayed hydroxyapatite coatings having different crystallinity.
Weichang Xue (2004)
Synthesis of functionally graded bioactive glass-apatite multistructures on Ti substrates by pulsed laser deposition
Dragan Tanasković (2007)
Efficient destruction of bacteria with Ti(IV) and antibacterial ions in co-substituted hydroxyapatite films
Chun Hu (2007)
Antibacterial nanosized silver substituted hydroxyapatite: synthesis and characterization.
N. Rameshbabu (2007)
Influence of the hydrothermal temperature and pH on the crystallinity of a sputtered hydroxyapatite film
Kazuhide Ozeki (2010)
Synthesis, characterization and antimicrobial activity of copper and zinc-doped hydroxyapatite nanopowders
Vojislav Stanić (2010)
Antimicrobial effects of metal ions (Ag+, Cu2+, Zn2+) in hydroxyapatite
Tyson N. Kim (1998)
Characterization of macroporous carbonate-substituted hydroxyapatite bodies prepared in different phosphate solutions
Yoong Lee (2007)
Reconstruction of the frontal sinus and frontofacial skeleton with hydroxyapatite cement.
Craig D. Friedman (2000)
Formation and structure of zinc-substituted calcium hydroxyapatite
Fumiaki Miyaji (2005)
Calcium phosphates as substitution of bone tissues
María Vallet-Regí (2004)
The influence of foreign ions on the crystal lattice of hydroxyapatite upon heating
Zoltan Zyman (2006)
Nanodimensional and Nanocrystalline Apatites and Other Calcium Orthophosphates in Biomedical Engineering, Biology and Medicine
S. Dorozhkin (2009)
Antibacterial Activity and Mechanism of Action of the Silver Ion in Staphylococcus aureus and Escherichia coli
Woo Kyung Jung (2008)
Some physico-chemical aspects of hydroxylapatite
T. S. B. Narasaraju (1996)
Bacterial adhesion studies on titanium, titanium nitride and modified hydroxyapatite thin films
Y. L. Jeyachandran (2007)
Experimental design approach in the synthesis of hydroxyapatite by neutralization method
Ivana D Smičiklas (2005)
Antibacterial effect of silver nanoparticles deposited on corona-treated polyester and polyamide fabrics
Maja Radetić (2008)
Subchronic oral toxicity of silver nanoparticles
Y. Kim (2010)
Anti-Microbial Hydroxyapatite Particles Synthesized by a Sol–Gel Route
Ren-Jei Chung (2005)
Antimicrobial activity and action of silver.
A. Denver Russell (1994)
Bacterial resistance to silver in wound care.
Steven L. Percival (2005)
Calcium Orthophosphates in Nature, Biology and Medicine
S. Dorozhkin (2009)
Study of cytotoxicity mechanisms of silver nitrate in human dermal fibroblasts.
Eduard Hidalgo (1998)
In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating.
Wei Chen (2006)
The effect of hydroxyapatite coating on bony ingrowth into grooved titanium implants.
Kouu Hayashi (1999)
A historical review of the use of silver in the treatment of burns. II. Renewed interest for silver.
Henk J. Klasen (2000)
Preparation and antibacterial effect of silver–hydroxyapatite/titania nanocomposite thin film on titanium
A. Mo (2008)
This paper is referenced by
In vitro cytotoxicity and antibacterial activity of silver-coated electrospun polycaprolactone/gelatine nanofibrous scaffolds
Mim Mim Lim (2016)
A perspective on the hemolytic activity of chemical and green-synthesized silver and silver oxide nanoparticles
C Ashokraja (2017)
Facile hydrothermal synthesis of antibacterial multi-layered hydroxyapatite nanostructures with superior flexibility
Mei Qi (2018)
Anti-inflammatory effect of new calcium hydroxide paste containing silicon-substituted hydroxyapatite in lipopolysaccharide-stimulated macrophages
Jiyeon Roh (2018)
Cytotoxicity of hydroxyapatite coatings modified with silver nanoparticles
Joanna Sulej-Chojnacka (2012)
Control of Ag Release from Ag‐Containing Calcium Phosphates in Simulated Body Fluid
Ozkan Gokcekaya (2015)
Ion size, loading, and charge determine the mechanical properties, surface apatite, and cell growth of silver and tantalum doped calcium silicate
Seyed Farid Seyed Shirazi (2016)
Sol-gel combustion synthesis of Ag doped CaSiO3: in vitro bioactivity, antibacterial activity and cytocompatibility studies for biomedical applications
M. Venumadhava Reddy (2018)
Synthesis and Characterization of Fluorescent Hydroxyapatite Nanoparticles and Their Applications in Bioimaging and Plasmid Delivery
Ketaki Deshmukh (2016)
Antibacterial ability of supported silver nanoparticles by functionalized hydroxyapatite with 5-aminosalicylic acid
Vesna Lazić (2018)
Effect of dopant loading on the structural features of silver-doped hydroxyapatite obtained by mechanochemical method
Ashkan Fakharzadeh (2017)
The effect of hydroxyapatite nanoparticles on adipogenic differentiation of human mesenchymal stem cells.
Xing Yang (2018)
Substituted hydroxyapatites for bone repair
Jennifer H. Shepherd (2012)
Synthesis, structural analysis, mechanical, antibacterial and hemolytic activity of Mg²⁺ and Cu²⁺ co-substitutions in β-Ca₃(PO₄)₂.
Ram Kishore Singh (2014)
Preparation and characterization of novel Ag doped hydroxyapatite–Fe3O4–chitosan hybrid composites and in vitro biological evaluations for orthopaedic applications
U. Sri Anjaneyulu (2016)
Vacuum plasma sprayed coatings using ionic silver doped hydroxyapatite powder to prevent bacterial infection of bone implants.
Stefanie Guimond-Lischer (2016)
Improved surface morphology of silver/copper oxide/bentonite nanocomposite using aliphatic ammonium based ionic liquid for enhanced biological activities
Bama Krishnan (2017)
Quantification of silver ion release, in vitro cytotoxicity and antibacterial properties of nanostuctured Ag doped TiO2 coatings on stainless steel deposited by RF magnetron sputtering
K. Jamuna-Thevi (2011)
Synthesis; characterization and antimicrobial effects of composites based on multi-substituted hydroxyapatite and silver nanoparticles
Aurora Mocanu (2014)
Functionalized Silver Doped Hydroxyapatite Scaffolds for Controlled Simultaneous Silver Ion and Drug Delivery
Arita Dubņika (2017)
Nano-hydroxyapatite(n-HA) Involved in The Regeneration of Rat Nerve Injury Triggered by Overloading Stretch
Meili Liu (2019)
Biosynthesized Nanobullets for Microbes and Biofilms
Lubna Sherin (2018)
Phase transfer synthesis of N,N′(1,2-phenylene)bis-hippuricamide tethered metal based functionalized nanoparticles: A study on some novel microbial targeting peptide-mimic nanoparticles
Natarajan Raman (2011)
Badania naukowe z zakresu inżynierii biomedycznej realizowane w Instytucie Obróbki Plastycznej
Monika Gierzyńska-Dolna (2014)
Substitution of strontium and boron into hydroxyapatite crystals: Effect on physicochemical properties and biocompatibility with human Wharton-Jelly stem cells.
Joanna Kolmas (2017)
Bioactivity Assessment of Ag-HA
Flavia Mendonça Fonseca (2018)
Antioxidant and Antimicrobial Activity of Tellurium Dioxide Nanoparticles Sols
C. L. Zhong (2013)
Recent Nanotechnology Approaches for Prevention and Treatment of Biofilm-Associated Infections on Medical Devices
Mohankandhasamy Ramasamy (2016)
Production, thermal and dielectrical properties of Ag-doped nano-strontium apatite and nano h-BN filled poly(4-(3-(2,3,4-trimethoxyphenyl) acryloyl) phenyl acrylate) composites
Turan Gurgenc (2020)
Silver, magnesium and zinc substituted hydroxyapatite for orthopaedic applications
Maryam Askari Louyeh (2017)
Natural rubber–based composites filled with bioglasses from a CaO‐SiO2‐P2O5‐Ag2O system. Effect of Ag2O concentration in the filler on composite properties
Abdullah G. Al-Sehemi (2020)
Fabrication of calcium phosphate microcapsules using emulsion droplets stabilized with branched copolymers as templates.
Robert V. Bell (2015)See more