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Efficacy Of Enzymatic Mouth Rinses For Immobilisation Of Protective Enzymes In The In Situ Pellicle.

C. Hannig, B. Spies, B. Spitzmüller, M. Hannig
Published 2010 · Chemistry, Medicine

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AIM Mouth rinses containing enzymes are designed for patients suffering from xerostomia. The objective of the present in situ study was to investigate the efficacy of these rinses for targeted accumulation and immobilisation of protective enzymes in the acquired pellicle. METHODS A number of six healthy subjects carried bovine enamel slabs fixed on individual upper jaw splints for pellicle formation in situ. After 1 min, they rinsed with biotène or BioXtra for 10 min, respectively. Enzyme activities of lysozyme, peroxidase and glucoseoxidase in the in situ pellicle and in the saliva were assayed before as well as 0, 20 and 40 min after the rinses. The assays for the respective enzyme activities were based on fluorogenic substrates. Separate experiments were performed for the different enzymes and mouth rinses, respectively. Statistical evaluation was carried out with the Kruskal-Wallis test. RESULTS None of the investigated rinses had any significant impact on the activities of lysozyme, peroxidase and glucoseoxidase detectable in the in situ pellicle or in the saliva (Kruskal-Wallis test, p>0.05). Despite the fact that both products should contain lactoperoxidase activity according to manufacturers' instructions, no peroxidase activity was measurable in the pure mouth rinses. CONCLUSION With the tested enzymatic mouth rinses targeted accumulation and immobilisation of protective enzymes in the in situ pellicle did not seem possible.
This paper references
10.1111/J.1875-595X.2000.TB00554.X
Saliva in health and disease: an appraisal and update.
L. Sreebny (2000)
10.1042/BJ2840557
Formation of salivary-mucosal pellicle: the role of transglutaminase.
S. Bradway (1992)
10.1177/00220345690480053501
The Interaction of Salivary Proteins with Tooth Surface
K. Pruitt (1969)
10.1093/OXFORDJOURNALS.JBCHEM.A133073
A new lysozyme assay based on fluorescence polarization or fluorescence intensity utilizing a fluorescent peptidoglycan substrate.
H. Maeda (1980)
10.1111/J.1600-0722.2004.00166.X
Ellipsometry analysis of the in vitro adsorption of tea polyphenols onto salivary pellicles.
A. Joiner (2004)
10.1016/0003-9969(83)90045-6
Synergism of lysozyme, proteases and inorganic monovalent anions in the bacteriolysis of oral Streptococcus mutans GS5.
J. J. Pollock (1983)
Dry mouth and salivary gland hypofunction, Part III: Treatment.
Sreebny Lm (1988)
Human saliva : clinical chemistry and microbiology
J. Tenovuo (1989)
10.1016/j.archoralbio.2007.12.003
Intrinsic enzymatic crosslinking and maturation of the in situ pellicle.
C. Hannig (2008)
Salivary flow in health and disease.
Sreebny Lm (1989)
10.1016/0003-2697(84)90409-3
Purification of human lysozyme from milk and pancreatic juice.
C. Wang (1984)
10.1111/j.1365-3083.1980.tb00220.x
A New Quantitative Fluorimetric Assay for Phagocytosis of Bacteria
B. Vray (1980)
10.1016/J.FOODCHEM.2006.11.074
Partial purification and preparation of bovine lactoperoxidase and characterization of kinetic properties of its immobilized form incorporated into cross-linked alginate films
Çiğdem Mecitoğlu (2007)
10.1021/BI9700328
Multiple interactions between polyphenols and a salivary proline-rich protein repeat result in complexation and precipitation.
N. Baxter (1997)
10.1111/J.1600-0722.2004.00168.X
Electron microscopic detection of salivary α-amylase in the pellicle formed in situ
D. Deimling (2004)
10.1002/HLCA.19710540830
Human milk lysozyme: unpublished data concerning the establishment of the complete primary structure; comparison with lysozymes of various origins.
J. Jollés (1971)
Electron microscopic detection of salivary alpha-amylase in the pellicle formed in situ.
D. Deimling (2004)
10.1128/IAI.45.3.610-617.1984
In vitro and in vivo studies of cellular lysis of oral bacteria by a lysozyme-protease-inorganic monovalent anion antibacterial system.
J. J. Pollock (1984)
10.1159/000084031
Effect of Cevimeline on Salivary Components in Patients with Sjögren Syndrome
K. Suzuki (2005)
10.1016/j.jdent.2008.04.002
Effects of Cistus-tea on bacterial colonization and enzyme activities of the in situ pellicle.
C. Hannig (2008)
10.1055/S-2007-998199
[Sialochemical studies in Sjögren disease].
S. Menstell (1990)
10.1111/J.1600-0722.2004.00180.X
Enzymes in the acquired enamel pellicle.
C. Hannig (2005)
Nonimmunoglobulin defence factors in human saliva
J. Tenovuo (1989)
10.1007/s00784-004-0284-1
Transmission electron microscopy comparison of methods for collecting in situ formed enamel pellicle
M. Hannig (2004)
10.1016/0014-5793(96)00186-X
Tannin interactions with a full‐length human salivary proline‐rich protein display a stronger affinity than with single proline‐rich repeats
A. Charlton (1996)
10.1007/s00253-008-1407-4
Glucose oxidase: natural occurrence, function, properties and industrial applications
C. M. Wong (2008)
10.1002/JBM.A.30758
Influence of different restorative materials on lysozyme and amylase activity of the salivary pellicle in situ.
C. Hannig (2006)
Immobilisation and activity of human alpha-amylase in the acquired enamel pellicle.
C. Hannig (2004)
10.1159/000261552
Effects of a lactoperoxidase system-containing toothpaste on levels of hypothiocyanite and bacteria in saliva.
M. Lenander-Lumikari (1993)
10.1159/000090585
The structure, function and properties of the acquired pellicle.
M. Hannig (2006)
10.1016/j.archoralbio.2008.03.003
Detection and activity of peroxidase in the in situ formed enamel pellicle.
C. Hannig (2008)
10.1007/s00784-009-0357-2
Targeted immobilisation of lysozyme in the enamel pellicle from different solutions
C. Hannig (2009)
10.1177/154405910208101202
Antimicrobial Agents in Saliva—Protection for the Whole Body
J. Tenovuo (2002)
10.1016/j.biotechadv.2009.04.003
Glucose oxidase--an overview.
S. Bankar (2009)
10.1016/J.ARCHORALBIO.2003.11.007
Salivary micelles: identification of complexes containing MG2, sIgA, lactoferrin, amylase, glycosylated proline-rich protein and lysozyme.
R. V. Soares (2004)
10.1001/ARCHINTE.147.7.1333
Xerostomia. A neglected symptom.
L. Sreebny (1987)
10.1177/08959374000140010301
Saliva and Dental Pellicle-A Review
U. Lendenmann (2000)
10.1016/0167-4838(94)90218-6
Inactivation of horseradish peroxidase by phenol and hydrogen peroxide: a kinetic investigation.
K. J. Baynton (1994)
10.1016/J.ARCHORALBIO.2007.05.006
Fluorescence microscopic visualization and quantification of initial bacterial colonization on enamel in situ.
C. Hannig (2007)
10.1007/s00784-008-0213-9
Characterisation of lysozyme activity in the in situ pellicle using a fluorimetric assay
C. Hannig (2008)
10.1016/J.ARCHORALBIO.2006.01.006
Microradiographic study on the effects of mucin-based solutions used as saliva substitutes on demineralised bovine enamel in vitro.
H. Meyer-Lueckel (2006)
10.1034/J.1601-0825.2002.1O781.X
Clinical applications of antimicrobial host proteins lactoperoxidase, lysozyme and lactoferrin in xerostomia: efficacy and safety.
J. Tenovuo (2002)
10.1177/08959374960100010201
The Use of Whole Saliva in the Differential Diagnosis of Sjögren's Syndrome
L. Sreebny (1996)
Effect of Irradiation of Head and Neck Cancer on the Enamel Salivary Pellicle
E Dounis (2003)
10.1177/00220345870660021401
Lysozyme-Protease-Inorganic Monovalent Anion Lysis of Oral Bacterial Strains in Buffers and Stimulated Whole Saliva
J. J. Pollock (1987)
10.1159/000057869
Lactoperoxidase Inhibits Glucosyltransferases from Streptococcus mutans in vitro
A. Korpela (2002)
10.1016/j.jdent.2008.01.010
The protective nature of pellicle towards toothpaste abrasion on enamel and dentine.
A. Joiner (2008)
10.1128/IAI.48.3.720-728.1985
Bactericidal activity of human lysozyme, muramidase-inactive lysozyme, and cationic polypeptides against Streptococcus sanguis and Streptococcus faecalis: inhibition by chitin oligosaccharides.
N. Laible (1985)
10.3109/00016359409029032
Effects of a lactoperoxidase-system-containing toothpaste on dental plaque and whole saliva in vivo.
V. Kirstilä (1994)
10.1016/J.ARCHORALBIO.2004.01.005
Immobilisation and activity of human α-amylase in the acquired enamel pellicle
C. Hannig (2004)
10.1016/S0014-5793(97)01621-9
Adsorption of human lysozyme onto hydroxyapatite
T. Aizawa (1998)
[Use of saliva substitutes in patients with xerostomia].
H. Meyer-Lueckel (2002)
10.1159/000077762
Salivary Proteins: Protective and Diagnostic Value in Cariology?
A. van Nieuw Amerongen (2004)
Dry mouth and salivary gland hypofunction, Part I: Diagnosis.
L. Sreebny (1988)
10.1034/J.1601-0825.2002.01762.X
The effect of commercially available saliva substitutes on predemineralized bovine dentin in vitro.
H. Meyer-Lueckel (2002)
10.1099/JMM.0.47094-0
The in vivo dynamics of Streptococcus spp., Actinomyces naeslundii, Fusobacterium nucleatum and Veillonella spp. in dental plaque biofilm as analysed by five-colour multiplex fluorescence in situ hybridization.
A. Al-Ahmad (2007)
10.1016/j.jdent.2009.03.017
Polyphenolic beverages reduce initial bacterial adherence to enamel in situ.
C. Hannig (2009)
10.1016/0165-022X(94)90065-5
A fluorometric assay of peroxidase activity utilizing 2',7'-dichlorofluorescein with thiocyanate: application to the study of salivary secretion.
G. Proctor (1994)
Caries and periodontal conditions in patients with primary Sjögren's syndrome.
N. Ravald (1998)
10.1007/s00784-005-0026-z
Does irradiation affect the protein composition of saliva?
M. Hannig (2005)
10.1021/BI00401A035
Purification and characterization of human salivary peroxidase.
B. Månsson-Rahemtulla (1988)
10.1034/J.1600-0722.2003.00073.X
Adsorption from black tea and red wine onto in vitro salivary pellicles studied by ellipsometry.
A. Joiner (2003)



This paper is referenced by
10.3390/ijms14010920
Proteome and Peptidome of Human Acquired Enamel Pellicle on Deciduous Teeth
Jason N. Zimmerman (2013)
10.1002/biot.201200313
Antimicrobial enzymes: An emerging strategy to fight microbes and microbial biofilms
Barbara Thallinger (2013)
10.1007/s00784-012-0734-0
Do edible oils reduce bacterial colonization of enamel in situ?
C. Hannig (2012)
10.1177/0022034512462578
New Insights into the Composition and Functions of the Acquired Enamel Pellicle
W. Siqueira (2012)
10.1038/s41405-018-0005-5
The antibacterial efficacy of a foam mouthwash and its ability to remove biofilms
S. B. Jones (2018)
10.1016/j.archoralbio.2013.06.015
Interactions between hyaluronic acid, lysozyme, and the glucose oxidase-mediated lactoperoxidase system in enzymatic and candidacidal activities.
Minah Cho (2013)
10.1159/000344016
Reduction of Erosion by Protein-Containing Toothpastes
D. J. Jager (2012)
10.1016/j.jdent.2018.10.010
Microbiology insights into boosting salivary defences through the use of enzymes and proteins.
A. Cawley (2019)
10.1016/j.archoralbio.2011.11.007
Candidacidal activities of the glucose oxidase-mediated lactoperoxidase system.
H. Kho (2012)
10.3390/ijms20061443
The Significance of Lactoperoxidase System in Oral Health: Application and Efficacy in Oral Hygiene Products
Marcin Magacz (2019)
10.1111/idh.12226
Oral hygiene grade and quality of life in children with chemotherapy-related oral mucositis: a randomized study on the impact of a fluoride toothpaste with salivary enzymes, essential oils, proteins and colostrum extract versus a fluoride toothpaste without menthol.
E. Bardellini (2016)
10.1016/j.archoralbio.2019.05.017
Activity and distribution pattern of enzymes in the in-situ pellicle of children.
Susann Hertel (2019)
10.1016/j.jdent.2017.05.011
Oral astringent stimuli alter the enamel pellicle's ultrastructure as revealed by electron microscopy.
Melanie Rehage (2017)
10.1016/j.archoralbio.2018.02.007
Effects of molecular weight of hyaluronic acid on its viscosity and enzymatic activities of lysozyme and peroxidase.
J. Kim (2018)
10.1016/j.archoralbio.2010.03.020
Efficacy of enzymatic toothpastes for immobilisation of protective enzymes in the in situ pellicle.
C. Hannig (2010)
10.1016/J.MEDCLI.2013.02.036
Pronóstico y tratamiento de la boca seca. Revisión sistemática
J. López-López (2014)
10.1007/s00253-014-5889-y
Bromelain: an overview of industrial application and purification strategies
Z. I. M. Arshad (2014)
10.1159/000452226
Enzymology and Ultrastructure of the in situ Pellicle in Caries-Active and Caries-Inactive Patients
J. Kirsch (2017)
10.1159/000325945
In vitro and in situ Erosion Models for Evaluating Tooth Substance Loss
N. West (2011)
10.1007/s00784-012-0781-6
Influence of a mouthwash containing hydroxyapatite microclusters on bacterial adherence in situ
C. Hannig (2012)
10.1016/j.jdent.2018.12.002
A randomised clinical trial to determine the effect of a toothpaste containing enzymes and proteins on gum health over 3 months.
S. Daly (2019)
10.1111/eos.12207
Enzymes in the in-situ pellicle of children with different caries activity.
S. Grychtol (2015)
10.1016/j.ijbiomac.2015.05.052
(1→3)-α-D-Glucan hydrolases in dental biofilm prevention and control: A review.
M. Pleszczyńska (2015)
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