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Effects Of Molecular Weight Of Hyaluronic Acid On Its Viscosity And Enzymatic Activities Of Lysozyme And Peroxidase.

J. Kim, Ji-Youn Chang, Yoonyoung Kim, Moon-Jong Kim, H. Kho
Published 2018 · Chemistry, Medicine

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OBJECTIVES To investigate the effects of the molecular weight of hyaluronic acid on its viscosity and enzymatic activities of lysozyme and peroxidase in solution and on the hydroxyapatite surface. DESIGN Hyaluronic acids of four different molecular weights (10 kDa, 100 kDa, 1 MDa, and 2 MDa), hen egg-white lysozyme, bovine lactoperoxidase, and human whole saliva were used. Viscosity values of hyaluronic acids were measured using a cone-and-plate viscometer at six different concentrations (0.1-5.0 mg/mL). Enzymatic activities of lysozyme and peroxidase were examined by hydrolysis of fluorescein-labeled Micrococcus lysodeikticus and oxidation of fluorogenic 2',7'-dichlorofluorescein to fluorescing 2',7'-dichlorofluorescein, respectively. RESULTS In solution assays, only 2 MDa-hyaluronic acid significantly inhibited lysozyme activities in saliva. In surface assays, hyaluronic acids inhibited lysozyme and peroxidase activities; the inhibitory activities were more apparent with high-molecular-weight ones in saliva than in purified enzymes. The 100 kDa-hyaluronic acid at 5.0 mg/mL, 1 MDa-one at 0.5 mg/mL, and 2 MDa-one at 0.2 mg/mL showed viscosity values similar to those of human whole saliva at a shear rate range required for normal oral functions. The differences among the influences of the three conditions on the enzymatic activities were not statistically significant. CONCLUSIONS High-molecular-weight hyaluronic acids at low concentration and low-molecular-weight ones at high concentration showed viscosity values similar to those of human whole saliva. Inhibitory effects of hyaluronic acids on lysozyme and peroxidase activities were more significant with high-molecular-weight ones on the surface and in saliva compared with in solution and on purified enzymes.
This paper references
10.1186/AR623
Intra-articular hyaluronan (hyaluronic acid) and hylans for the treatment of osteoarthritis: mechanisms of action
L. Moreland (2003)
10.1006/ABBI.1997.0365
Dependence of salt concentration on glycosaminoglycan-lysozyme interactions in cartilage.
J. M. Moss (1997)
10.1002/(SICI)1097-4636(20000615)50:4<574::AID-JBM13>3.0.CO;2-I
Synthesis and characterization of polypyrrole-hyaluronic acid composite biomaterials for tissue engineering applications.
J. Collier (2000)
10.1016/S0003-9969(96)00050-7
Hyaluronan (hyaluronic acid) in human saliva.
M. Pogrel (1996)
10.1177/0885328212446936
The effects of hyaluronic acid incorporated as a wetting agent on lysozyme denaturation in model contact lens materials
Andrea Weeks (2013)
10.1177/154411130301400507
Molecular recognition at the protein-hydroxyapatite interface.
P. Stayton (2003)
10.1016/0021-9797(88)90402-X
Conformational states of enzymes bound to surfaces
R. Sandwick (1988)
10.1002/ANR.1780320416
Changes in the viscosity of hyaluronic acid after exposure to a myeloperoxidase-derived oxidant.
M. S. Baker (1989)
10.1111/ger.12220
Evaluation of the effectiveness of a chamomile (Matricaria chamomilla) and linseed (Linum usitatissimum) saliva substitute in the relief of xerostomia in elders
I. Morales-Bozo (2017)
10.1007/s00066-005-1333-7
Different Saliva Substitutes for Treatment of Xerostomia Following Radiotherapy
F. Momm (2005)
10.1111/J.1601-0825.2006.01263.X
Viscosity and wettability of animal mucin solutions and human saliva.
M. Park (2007)
10.1016/0165-022X(94)90065-5
A fluorometric assay of peroxidase activity utilizing 2',7'-dichlorofluorescein with thiocyanate: application to the study of salivary secretion.
G. Proctor (1994)
10.1111/j.1601-0825.2011.01807.x
Influences of hyaluronic acid on the anticandidal activities of lysozyme and the peroxidase system.
J. Kang (2011)
10.2334/JOSNUSD.45.85
Hyaluronan (hyaluronic acid) and its regulation in human saliva by hyaluronidase and its inhibitors.
M. Pogrel (2003)
10.1016/S0300-9785(83)80048-9
A clinical comparison between commercially available mucin- and CMC-containing saliva substitutes.
A. Vissink (1983)
10.1111/j.1601-0825.2009.01650.x
Rheological properties of hyaluronic acid and its effects on salivary enzymes and candida.
M. Park (2010)
10.1016/0301-4622(90)85009-U
Modulation of egg-white lysozyme activity by viscosity intensifier additives.
L. Lamy (1990)
10.1016/S0300-9785(86)80027-8
A double-blind crossover trial of CMC- and mucin-containing saliva substitutes.
L. L. Visch (1986)
10.1006/ABBI.1994.1134
Binding properties of glycosaminoglycans to lysozyme--effect of salt and molecular weight.
M. van Damme (1994)
10.1016/S0168-3659(00)00300-X
Cross-linked hyaluronic acid hydrogel films: new biomaterials for drug delivery.
Y. Luo (2000)
10.1016/j.ijom.2008.06.006
Effects of carboxymethylcellulose (CMC)-based artificial saliva in patients with xerostomia.
D.-J. Oh (2008)
10.1111/j.1475-1313.2009.00683.x
Efficacy of different dry eye treatments with artificial tears or ocular lubricants: a systematic review
M. Doughty (2009)
Influences of Saliva Substitutes on Salivary Enzymatic Activity
H. Kho (2009)
10.1016/j.oooo.2014.12.005
Efficacy and safety of a new oral saliva equivalent in the management of xerostomia: a national, multicenter, randomized study.
Michel Salom (2015)
10.1042/BJ1520057
Inhibition of leucocytic lysosomal enzymes by glycosaminoglycans in vitro.
J. L. Avila (1975)
10.2220/BIOMEDRES.30.63
Salivary levels of hyaluronic acid in female patients with dry mouth compared with age-matched controls: a pilot study.
Y. Higuchi (2009)
Depolymerization of synovial fluid hyaluronic acid (HA) by the complete myeloperoxidase (MPO) system may involve the formation of a HA-MPO ionic complex.
S. P. Green (1990)
10.1016/S0927-7765(02)00160-1
Interactions of phospholipid- and poly(ethylene glycol)-modified surfaces with biological systems: Relation to physico-chemical properties and mechanisms
Patrick Vermette (2003)
10.1111/J.1600-0722.1991.TB01034.X
Objective and subjective efficacy of saliva substitutes containing mucin and carboxymethylcellulose.
H. Olsson (1991)
10.1016/j.archoralbio.2009.10.004
Efficacy of enzymatic mouth rinses for immobilisation of protective enzymes in the in situ pellicle.
C. Hannig (2010)
10.1016/J.COLSURFB.2006.11.024
Protein release behavior from porous microparticle with lysozyme/hyaluronate ionic complex.
E. Lee (2007)
10.1021/bm100861g
Rodlike complexes of a polyelectrolyte (hyaluronan) and a protein (lysozyme) observed by SANS.
I. Morfin (2011)
10.1016/j.ejpb.2014.09.001
Complex coacervates of hyaluronic acid and lysozyme: effect on protein structure and physical stability.
J. Water (2014)
10.1016/j.oooo.2016.09.008
Management of dry mouth: assessment of oral symptoms after use of a polysaccharide-based oral rinse.
J. Epstein (2017)
10.1111/J.1600-0714.1984.TB01397.X
Rheological properties of saliva substitutes containing mucin, carboxymethylcellulose or polyethylenoxide.
A. Vissink (1984)
Binding of hyaluronan to lysozyme at various pHs and salt concentrations.
M. van Damme (1991)
10.1038/sj.bdj.4806731
A double-blind cross-over trial of a mucin-containing artificial saliva
A. Duxbury (1989)
10.1111/ger.12000
Yam tuber mucilage as a candidate substance for saliva substitute: in vitro study of its viscosity and influences on lysozyme and peroxidase activities.
H. Kho (2014)
10.1016/j.archoralbio.2008.03.003
Detection and activity of peroxidase in the in situ formed enamel pellicle.
C. Hannig (2008)
10.1177/10454411930040030401
Development of artificial salivas.
M. Levine (1993)
10.1016/S0737-0806(85)80102-7
Sodium hyaluronate and joint function
E. Balazs (1985)
10.1111/J.1741-2358.1987.TB00283.X
The efficacy of mucin-containing artificial saliva in alleviating symptoms of xerostomia.
A. Vissink (1987)
10.1080/13693780601039607
Potential role of high molecular weight hyaluronan in the anti-Candida activity of human oral epithelial cells.
A. Sakai (2007)



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