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Targeted Intracellular Codelivery Of Chemotherapeutics And Nucleic Acid With A Well-defined Dendrimer-based Nanoglobular Carrier.

Todd L Kaneshiro, Zheng-Rong Lu
Published 2009 · Materials Science, Medicine

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Codelivery of different therapeutics has a potential to efficaciously treat human diseases via their synergetic effects. We have recently developed a new class dendrimers, poly(l-lysine) dendrimers with a silsesquioxane cubic core (nanoglobules). These dendrimers have compact globular and well-defined structures and highly functionalized surfaces, and can be used as versatile carriers for biomedical applications. In this study, a generation-3 (G3) nanoglobular dendrimer was used to conjugate a peptide c(RGDfK) with a PEG spacer for codelivery of doxorubicin (DOX) and siRNA. Doxorubicin (DOX) was coupled to the RGD targeted nanoglobule via a degradable disulfide spacer to give G3-[PEG-RGD]-[DOX]. G3-[PEG-RGD]-[DOX] showed higher cytotoxicity than free DOX at high doses in glioblastoma U87 cells. G3-[PEG-RGD]-[DOX] was further complexed with siRNA and such complexes were readily internalized by U87 cells as shown by confocal microscopy. The siRNA complexes of the targeted conjugate resulted in significantly higher gene silencing efficiency in U87-Luc cells than those of control conjugates G3-[PEG-RGD] and G3-[DOX]. The nanoglobules are promising carriers for the codelivery of nucleic acids and chemotherapeutic agents.
This paper references
Controlled cleavage of R8Si8O12 frameworks: a revolutionary new method for manufacturing precursors to hybrid inorganic–organic materials
F. Feher (1998)
Synthesis, characterization, and biological evaluation of integrin alphavbeta3-targeted PAMAM dendrimers.
C. Boswell (2008)
Preparation of a Promising Angiogenesis PET Imaging Agent: 68Ga-Labeled c(RGDyK)–Isothiocyanatobenzyl-1,4,7-Triazacyclononane-1,4,7-Triacetic Acid and Feasibility Studies in Mice
J. Jeong (2008)
A critical evaluation of the mechanisms of action proposed for the antitumor effects of the anthracycline antibiotics adriamycin and daunorubicin.
D. Gewirtz (1999)
Polyhedral Oligomeric Silsesquioxane Nanocomposites: The Next Generation Material for Biomedical Applications
R. Kannan (2006)
Synthesis, characterization, and gene delivery of poly-L-lysine octa(3-aminopropyl)silsesquioxane dendrimers: nanoglobular drug carriers with precisely defined molecular architectures.
Todd L Kaneshiro (2007)
Electrochemo-gene therapy of cancer: intratumoral delivery of interleukin-12 gene and bleomycin synergistically induced therapeutic immunity and suppressed subcutaneous and metastatic melanomas in mice.
T. Kishida (2003)
Self-assembled polyethylenimine-graft-poly(epsilon-caprolactone) micelles as potential dual carriers of genes and anticancer drugs.
L. Y. Qiu (2007)
Self-assembled polyethylenimine-graft-poly(ε-caprolactone) micelles as potential dual carriers of genes and anticancer drugs
L. Y. Qiu (2007)
Co-delivery of drugs and DNA from cationic core–shell nanoparticles self-assembled from a biodegradable copolymer
Y. Wang (2006)
Targeted Delivery of Doxorubicin by HPMA Copolymer-Hyaluronan Bioconjugates
Yi Luo (2004)
Dual-Function Probe for PET and Near-Infrared Fluorescence Imaging of Tumor Vasculature
W. Cai (2007)
Synthesis of DOTA-conjugated multivalent cyclic-RGD peptide dendrimers via 1,3-dipolar cycloaddition and their biological evaluation: implications for tumor targeting and tumor imaging purposes.
I. Dijkgraaf (2007)
Induction of apoptosis in MDR1 expressing cells by daunorubicin with combinations of suboptimal concentrations of P-glycoprotein modulators.
A. Aszalos (2001)
Quantitating intracellular transport of polyplexes by spatio-temporal image correlation spectroscopy.
R. Kulkarni (2005)
PET/MRI Dual-Modality Tumor Imaging Using Arginine-Glycine-Aspartic (RGD)–Conjugated Radiolabeled Iron Oxide Nanoparticles
Ha-young Lee (2008)
Conjugation to hyperbranched polyglycerols improves RGD-mediated inhibition of platelet function in vitro.
J. G. Zhang (2008)
Development of solid lipid nanoparticles containing ionically complexed chemotherapeutic drugs and chemosensitizers.
H. L. Wong (2004)
Platelet and endothelial adhesion on fluorosurfactant polymers designed for vascular graft modification.
Chad Tang (2009)
In vitro investigation of ionic polysaccharide microspheres for simultaneous delivery of chemosensitizer and antineoplastic agent to multidrug-resistant cells.
Z. Liu (1999)
Targeted systemic delivery of a therapeutic siRNA with a multifunctional carrier controls tumor proliferation in mice.
X. Wang (2009)
A novel environment-sensitive biodegradable polydisulfide with protonatable pendants for nucleic acid delivery.
X. Wang (2007)
Tumor-specific anti-nucleosome antibody improves therapeutic efficacy of doxorubicin-loaded long-circulating liposomes against primary and metastatic tumor in mice.
T. Elbayoumi (2009)
Liberation of doxorubicin from HPMA copolymer conjugate is essential for the induction of cell cycle arrest and nuclear fragmentation in ovarian carcinoma cells.
A. Malugin (2007)
Combination of local, nonviral IL12 gene therapy and systemic paclitaxel treatment in a metastatic breast cancer model.
M. Janát-Amsbury (2004)
Comparison of the Anticancer Effect of Free and HPMA Copolymer-Bound Adriamycin in Human Ovarian Carcinoma Cells
T. Minko (2004)
Contrast-Enhanced MRI-Guided Photodynamic Cancer Therapy with a Pegylated Bifunctional Polymer Conjugate
Anagha Vaidya (2008)
Synthesis and evaluation of globular Gd-DOTA-monoamide conjugates with precisely controlled nanosizes for magnetic resonance angiography.
Todd L Kaneshiro (2008)
Efficient active transport of gene nanocarriers to the cell nucleus
Junghae Suh (2003)
PDGF-regulated rab4-dependent recycling of alphavbeta3 integrin from early endosomes is necessary for cell adhesion and spreading.
M. Roberts (2001)
Repetitive doxorubicin treatment of glioblastoma enhances the PGP expression--a special role for endothelial cells.
M. Rittierodt (2003)
Synthesis and evaluation of globular Gd-DOTA-monoamide conjugates with precisely controlled nanosizes for magnetic resonance angiography.
Todd L Kaneshiro (2008)

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Codelivery of doxorubicin and p53 by biodegradable micellar carriers based on chitosan derivatives
Guan-Hai Wang (2015)
Enhanced Intestinal Permeability of Bufalin by a Novel Bufalin-Peptide-Dendrimer Inclusion through Caco-2 Cell Monolayer
C. Chan (2017)
Solidified Self-Nanoemulsifying Formulation for Oral Delivery of Combinatorial Therapeutic Regimen: Part II In vivo Pharmacokinetics, Antitumor Efficacy and Hepatotoxicity
A. Jain (2013)
Evaluation of Amino-Functional Polyester Dendrimers Based on Bis-MPA as Nonviral Vectors for siRNA Delivery
Patrik Stenström (2018)
Use of Nanotechnology to Develop Multi-Drug Inhibitors For Cancer Therapy
R. Gowda (2013)
UTMD-Promoted Co-Delivery of Gemcitabine and miR-21 Inhibitor by Dendrimer-Entrapped Gold Nanoparticles for Pancreatic Cancer Therapy
Lizhou Lin (2018)
RNAi-combined nano-chemotherapeutics to tackle resistant tumors.
Rakesh K Tekade (2016)
Synthesis and Evaluation of a Targeted Nanoglobular Dual-Modal Imaging Agent for MR Imaging and Image-Guided Surgery of Prostate Cancer
M. Tan (2013)
Theranostic Applications of Nanomaterials in Cancer: Drug Delivery, Image-Guided Therapy, and Multifunctional Platforms
A. Fernandez-Fernandez (2011)
Molecular Determinants of the Cellular Entry of Asymmetric Peptide Dendrimers and Role of Caveolae
Prarthana V. Rewatkar (2016)
Three-dimensional DNA nanostructures for dual-color microRNA imaging in living cells via hybridization chain reaction.
Mengmei Lv (2020)
Rational design of multifunctional magnetic mesoporous silica nanoparticle for tumor-targeted magnetic resonance imaging and precise therapy.
W. Chen (2016)
Comparing dendritic and self-assembly strategies to multivalency--RGD peptide-integrin interactions.
Daniel J. Welsh (2011)
Novel water soluble dendrimer nanocarrier for enhanced photodynamic efficacy of protoporphyrin IX
M. R. Suresh Kumar (2012)
Chimeric Nanoparticle: A Platform for Simultaneous Targeting of Phosphatidylinositol-3-Kinase Signaling and Damaging DNA in Cancer Cells.
Sandeep Palvai (2015)
Photosensitizer (PS)/polyhedral oligomeric silsesquioxane (POSS)-crosslinked nanohybrids for enhanced imaging-guided photodynamic cancer therapy.
Ya-Xuan Zhu (2017)
Co-delivery of doxorubicin and TRAIL plasmid by modified PAMAM dendrimer in colon cancer cells, in vitro and in vivo evaluation
Elham Pishavar (2019)
Nanotechnology-based intelligent drug design for cancer metastasis treatment.
Yu Gao (2014)
Dendrimers as Transfection Agents
C. Turrin (2011)
Enhanced cellular uptake of protoporphyrine IX/linolenic acid-conjugated spherical nanohybrids for photodynamic therapy.
Hye-in Lee (2016)
Multifunctional gadolinium-based dendritic macromolecules as liver targeting imaging probes.
Kui Luo (2011)
Dendrimers in anticancer drug delivery: mechanism of interaction of drug and dendrimers
J. Singh (2016)
Non‐covalent formulation of active principles with dendrimers: Current state‐of‐the‐art and prospects for further development
I. El’kin (2017)
Dual environment-responsive polyplex carriers for enhanced intracellular delivery of plasmid DNA.
Mai Sanjoh (2012)
Unraveling the photoluminescence puzzle of PAMAM dendrimers.
S. Lin (2011)
xorubicin and shRNA of Beclin 1 by folate receptor targeted pullulan-based multifunctional nanomicelles for combinational cancer therapy
Li-li Chen (2018)
Biodegradable nanoparticles composed of dendrigraft poly-L-lysine for gene delivery.
Y. Kodama (2014)
Small interfering RNAs (siRNAs) in cancer therapy: a nano-based approach
Ghanbar Mahmoodi Chalbatani (2019)
Nanotherapy for Cancer: Targeting and Multifunctionality in the Future of Cancer Therapies
A. Ediriwickrema (2015)
"Dendrimers are now emerging as nonviral vectors for siRNA, the wide range of chemical variation introducing the possibility of improved, (perfectly shaped) siRNA delivery complexes in the near future."
Olivia M Merkel (2010)
Drug-conjugated polymers as gene carriers for synergistic therapeutic effect.
P. Pofali (2016)
Nanocarrier mediated delivery of siRNA/miRNA in combination with chemotherapeutic agents for cancer therapy: current progress and advances.
N. S. Gandhi (2014)
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