Online citations, reference lists, and bibliographies.
← Back to Search

Toxin Detection By Si Photosensitive Biosensors With A New Measurement Scheme.

C. Park, Jong-Heon Yang, C. Ah, Chang-Geun Ahn, Yo Han Choi, K. H. Chung, W. Kim, G. Sung
Published 2011 · Materials Science, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
We propose a new type of photosensitive biosensor with a CMOS compatible Si photodiode integrated circuit, for the high-sensitive detection of small mycotoxin molecules requiring competitive assay approach. In this work, a photodiode is connected to the gate of a field effect transistor (FET) so that the open circuit voltage (V(OC)) of the illuminated photodiode is transferred into the drain/source current (I(DS)) of the FET. The sensing scheme employs competitive binding of toxin molecules (within the sample solution) and toxin-BSA conjugates (immobilized on the photodiode surface) with Au-nanoparticle-labeled antibodies, followed by silver enhancement to generate opaque structures on the photodiode surface. By utilizing the non-linear dependence of the V(OC) on the light intensity, we can maintain a sufficiently high signal resolution at low toxin concentrations (with most of the incident light blocked) for the competitive assay. By monitoring the I(DS) of the FET whose gate is driven by the V(OC), quantitative detection of Aflatoxin B1 has been achieved in the range of 0-15ppb.
This paper references
10.1093/ERAE/28.3.263
What Price Precaution? European Harmonisation of Aflatoxin Regulations and African Groundnut Exports
T. Otsuki (2001)
10.1016/J.SNB.2004.08.019
A DNA-detection platform with integrated photodiodes on a silicon chip
J. Li (2005)
10.1021/JF0354038
A rapid aflatoxin B1 ELISA: development and validation with reduced matrix effects for peanuts, corn, pistachio, and Soybeans.
N. Lee (2004)
10.1021/AC048957Y
Array biosensor for detection of ochratoxin A in cereals and beverages.
Miriam M. Ngundi (2005)
10.1016/J.BIOS.2005.10.021
Indirect competitive immunoassay for detection of aflatoxin B1 in corn and nut products using the array biosensor.
K. Sapsford (2006)
10.1039/c0lc00605j
Ultrasensitive protein detection using lithographically defined Si multi-nanowire field effect transistors.
Ruhai Tian (2011)
10.3390/s8096045
Sensors and Biosensors for the Determination of Small Molecule Biological Toxins
Xiang-hong Wang (2008)
10.1016/J.SNB.2009.11.056
Analysis of configuration of surface-immobilized proteins by Si nanochannel field effect transistor biosensor
C. Park (2011)
10.1109/TED.2007.896388
A CMOS Image Sensor Utilizing Opacity of Nanometallic Particles for DNA Detection
Yijin Wang (2007)
10.1364/AO.22.001780
Definition of the linearity range of Si photodiodes.
W. Budde (1983)
10.1016/j.bios.2009.12.026
Direct label-free electrical immunodetection in human serum using a flow-through-apparatus approach with integrated field-effect transistors.
Ansoon Kim (2010)
10.1021/ac100058f
Toward the limits of sandwich immunoassay of very low molecular weight molecules.
J. Quinton (2010)
10.1063/1.3486179
Photosensitive biosensor array system using optical addressing without an addressing circuit on array biochips
Chang-Geun Ahn (2010)
10.3390/s91108831
An Integrated ISFET Sensor Array
Kazuo Nakazato (2009)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar