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Self-assembled Carrageenan/protamine Polyelectrolyte Nanoplexes-Investigation Of Critical Parameters Governing Their Formation And Characteristics.
M. Dul, K. Paluch, Hazel Kelly, A. Healy, Astrid Sasse, L. Tajber
Published 2015 · Chemistry, Medicine
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The aim of this work was to investigate the feasibility of cross-linker free polyelectrolyte complex formation at the nanoscale between carrageenan (CAR) and protamine (PROT). The properties of CAR/PROT nanoparticles (NPs) were dependent on the carrageenan type: kappa (KC), iota (IC) and lambda (LC), concentration of components, addition of divalent cations, weight mixing ratio (WMR) of constituents and mode of component addition. In the case of 0.1% w/v solutions, IC-based NPs had the smallest particle sizes (100-150nm) and low polydispersity indices (0.1-0.4). A decrease in the solution concentration from 0.1% to 0.05% w/v enabled the formation of KC/PROT NPs. All carrageenans exhibited the ability to form NPs with surface charge ranging from -190 to 40mV. The inclusion of divalent cations caused an increase in the particle size and zeta potential. Infrared analysis confirmed the presence of a complex between CAR and PROT and showed that IC chains undergo structural changes when forming NPs. Colloidal stability of NPs was related to the initial surface charge of particles and was time- and pH-dependent. IC was found to be the most suitable type of CAR when forming nanoplexes with PROT.
This paper references
Polyelectrolyte multilayer assembly as a function of pH and ionic strength using the polysaccharides chitosan and heparin.
Soheil Boddohi (2008)
KC/PROT NPs, IC/PROT NPs and LC/PROT NPs. Band assignment was done based on studies of Bertoluzza et al
KC PROT (2010)
Polymeric Particulates to Improve Oral Bioavailability of Peptide Drugs
F. Delié (2005)
A note on the determination of the ester sulphate content of sulphated polysaccharides.
K. S. Dodgson (1962)
Dependency of polyelectrolyte complex stoichiometry on the order of addition 2. Aluminum chloride and poly-vinylsulfate
Junhua Chen (2004)
Physicochemical characterization of Remsima®
Soon Kwan Jung (2014)
Raman and infrared spectra of spermidine and spermine and their hydrochlorides and phosphates as a basis for the study of the interactions between polyamines and nucleic acids
A. Bertoluzza (1983)
Influence of pH and iota-carrageenan concentration on physicochemical properties and stability of beta-lactoglobulin-stabilized oil-in-water emulsions.
Y. S. Gu (2004)
OPTIMIZATION OF PROCESS VARIABLES FOR THE PREPARATION OF CHITOSANALGINATE NANOPARTICLES
V. Gupta (2011)
Miscibility study of carrageenan blends and evaluation of their effectiveness as sustained release carriers
S. Nanaki (2010)
Polyelectrolyte complex dispersions with a high colloidal stability controlled by the polyion structure and titrant addition rate
E. Dragan (2006)
An intra-articular salmon calcitonin-based nanocomplex reduces experimental inflammatory arthritis.
S. Ryan (2013)
Microencapsulation methods for delivery of protein drugs
Y. Yeo (2001)
Development and characterization of new insulin containing polysaccharide nanoparticles.
B. Sarmento (2006)
Dependency of polyelectrolyte complex stoichiometry on the order of addition. 1. Effect of salt concentration during streaming current titrations with strong poly-acid and poly-base
Junhua Chen (2003)
Isolation and characterization of soluble sulfated polysaccharide from the red seaweed Gracilaria cornea
Marcia R.S. Melo (2002)
Thermodynamic analysis of ion effects on the binding and conformational equilibria of proteins and nucleic acids: the roles of ion association or release, screening, and ion effects on water activity.
M. Record (1978)
Exploring the assembly process and properties of novel crosslinker-free hyaluronate-based polyelectrolyte complex nanocarriers.
A. Umerska (2012)
SOLID PHASE MICROBIAL TRANSFORMATION OF CORTEXOLONE AND PROLYL ENDOPEPTIDASE INHIBIOTORY ACTIVITY OF THE TRANSFORMED PRODUCTS
S. A. Shah (2011)
INTERACTION OF ACRIDINE ORANGE AND POLYANIONS: FLUORIMETRIC DETERMINATION OF BINDING STRENGTHS AND THE INFLUENCE OF SIMPLE ELECTROLYTES
R. Cundall (1979)
Self-assembled hyaluronate/protamine polyelectrolyte nanoplexes: synthesis, stability, biocompatibility and potential use as peptide carriers.
A. Umerska (2014)
Natural-origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications.
P. B. Malafaya (2007)
Physicochemical Characterization of Complex Drug Substances: Evaluation of Structural Similarities and Differences of Protamine Sulfate from Various Sources
David Awotwe-Otoo (2012)
Nanoparticles of quaternized chitosan derivatives as a carrier for colon delivery of insulin: ex vivo and in vivo studies.
A. Bayat (2008)
Protamine as an efficient membrane-translocating peptide.
F. Reynolds (2005)
KC/PROT NPs, IC/PROT NPs and LC/PROT NPs
KC PROT (1983)
Texture and rheological characterization of kappa and iota carrageenan in the presence of counter ions
T. R. Thrimawithana (2010)
Structural analysis of kappa-carrageenan isolated from Hypnea musciformis (red algae) and evaluation as an elicitor of plant defense mechanism
M. Arman (2012)
Physicochemical characterization of carrageenans—A critical reinvestigation
G. Berth (2008)
Carrageenans: biological properties, chemical modifications and structural analysis - a review.
V. Campo (2009)
Nanosizing of a drug/carrageenan complex to increase solubility and dissolution rate.
Wei-guo Dai (2007)
Optimisation of process variables for the preparation
V. K. Gupta (2011)
Oral bioavailability of insulin contained in polysaccharide nanoparticles.
B. Sarmento (2007)
Polysaccharides-based nanoparticles as drug delivery systems.
Z. Liu (2008)
The use of cell-penetrating peptides for drug delivery.
Jamal Temsamani (2004)
Novel polysaccharides-based nanoparticle carriers prepared by polyelectrolyte complexation for protein drug delivery
Yan Hu (2011)
An intra-articular salmon
P E. (2013)
Interaction of acridine orange and polyanions: fluorimetric determination of binding strengths and the influence of simple electrolytes
R. Cundall (1979)
In situ synthesis of magnetite nanoparticles in carrageenan gels.
A. L. Daniel-da-Silva (2007)
B. Sarmento (2007)
In-vitro and in-vivo evaluation of carrageenan/methylcellulose polymeric systems for transscleral delivery of macromolecules.
T. R. Thrimawithana (2011)
E. V. Shumilina (2002)
Characterization of self-assembled polyelectrolyte complex nanoparticles formed from chitosan and pectin.
N. P. Birch (2014)
Encapsulation of glucose oxidase (GOD) in polyelectrolyte complexes of chitosan-carrageenan
A. Briones (2010)
Chitosan-Carrageenan Polyelectrolyte Complex for the Delivery of Protein Drugs
Cunben Li (2013)
Polyelectrolyte multilayer assemblies.
M. C. Hsieh (1999)
Preparation and solid state characterisation of chlorothiazide sodium intermolecular self-assembly suprastructure.
K. Paluch (2010)
Thermodynamic analysis of ion
An overview of natural polymers for oral insulin delivery.
T. Sonia (2012)
Biodegradable nanoparticles for drug delivery and targeting
M. Hans (2002)
This paper is referenced by
Microbial Exopolysaccharides as Drug Carriers
A. Tabernero (2020)
Design of chondroitin sulfate-based polyelectrolyte nanoplexes: Formation of nanocarriers with chitosan and a case study of salmon calcitonin.
A. Umerska (2017)
Protamine and BSA–dextran complex emulsion improves oral bioavailability and anti-tumor efficacy of paclitaxel
Guangrui Xu (2020)
Self-assembled carbohydrate nanostructures: synthesis strategies to functional application in food
A. Roy (2016)
Preparation, evaluation and optimization of nanoparticles composed of thiolated triethyl chitosan: A potential approach for buccal delivery of insulin
Marzieh Rahbarian (2018)
Self-assembled nanocomplex of PEGylated protamine and heparin-suramin conjugate for accumulation at the tumor site.
Jooho Park (2018)
Development of Polyelectrolyte Complex Nanoparticles-PECNs Loaded with Ampicillin by Means of Polyelectrolyte Complexation and Ultra-High Pressure Homogenization (UHPH)
Nicolle Montero (2020)
Preparation of Water‐in‐Oil‐in‐Water Multiple Emulsions with Potential Use in Food Industry
I. Klojdová (2020)
Sulfated Seaweed Polysaccharides as Multifunctional Materials in Drug Delivery Applications
Ludmylla Cunha (2016)
An Enteric-Coated Polyelectrolyte Nanocomplex Delivers Insulin in Rat Intestinal Instillations When Combined with a Permeation Enhancer
Svenja Sladek (2020)
Optimisation of the self-assembly process: production of stable, alginate-based polyelectrolyte nanocomplexes with protamine
M. Dul (2017)
Carrageenan and More: Biorefinery Approaches with Special Reference to the Processing of Kappaphycus
Jose G. Ortiz-Tena (2017)
Seaweed Polysaccharide-Based Nanoparticles: Preparation and Applications for Drug Delivery
J. Venkatesan (2016)
Gelation mechanism and network structure of mixed kappa carrageenan/lambda carrageenan gels studied by macroscopic and microscopic observation methods
Lester C. Geonzon (2020)
Macroalgal Polysacharrides in Biomimetic Nanodelivery Systems.
N. Geškovski (2019)
pH-sensitive free AgNPs composite and nanocomposite beads based on starch as drug delivery systems
Shabnam Nezami (2019)
Development of a new type of multifunctional fucoidan-based nanoparticles for anticancer drug delivery.
Kun-Ying Lu (2017)
A novel approach to production of Chlorella protothecoides oil loaded nanoparticles via electrospraying method: Modelling of critical parameters for particle sizing.
Canan Yağmur Karakaş (2020)
Using polysaccharides for the enhancement of functionality of foods: A review
Xu Lu (2019)
Aggregation-induced photoluminescence enhancement of protamine-templated gold nanoclusters for 1-hydroxypyrene detection using 9-hydroxyphenanthrene as a sensitizer.
Jin-Hua Xue (2020)
Carrageenan-stabilized chitosan alginate nanoparticles loaded with ethionamide for the treatment of tuberculosis
S. Abdelghany (2017)
A review on synthesis, properties and applications of natural polymer based carrageenan blends and composites.
K. M. Zia (2017)
Starch-Chitosan Polyplexes: A Versatile Carrier System for Anti-Infectives and Gene Delivery
Hanzey Yasar (2018)
Protamine-oligonucleotide-nanoparticles: Recent advances in drug delivery and drug targeting.
B. Scheicher (2015)
Polysaccharides constructed hydrogels as vehicles for proteins and peptides. A review.
E. Dragan (2019)
Chondroitin-based nanoplexes as peptide delivery systems--Investigations into the self-assembly process, solid-state and extended release characteristics.
A. Umerska (2015)