← Back to Search
Deacetylation Affects The Physical Properties And Bioactivity Of Acemannan, An Extracted Polysaccharide From Aloe Vera.
Jaroenporn Chokboribal, Wanpen Tachaboonyakiat, P. Sangvanich, Vithaya Ruangpornvisuti, Suwimon Jettanacheawchankit, P. Thunyakitpisal
Published 2015 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
Acemannan, an acetylated polymannose from Aloe vera, induces tissue repair. We investigated the role of acemannan's acetyl-groups on its physical and biological properties. Deacetylated acemannan (DeAcAM) was prepared and characterized. The physical properties and microscopic structure of DeAcAM were evaluated using water solubility, contact angle, X-ray diffraction, and scanning-electron microscopy. The activity of DeAcAM on cell proliferation and gene expression were assessed. Acemannan and DeAcAM structures were simulated and the acemannan tetramer diad and its completely deacetylated structure were also determined. Increased acemannan deacetylation reduced its water solubility and hydrophilicity. Complete deacetylation altered acemannan's conformation to a partial crystal structure. The bioactivity of acemannan was reduced corresponding to its deacetylation. Acemannan induced cell proliferation, and VEGF and Collagen I expression; however, 100% DeAcAM did not. The simulated structures of the acemannan diad and the completely deacetylated diad were different. We conclude acetyl-groups affect acemannan's structure and physical/biological properties.
This paper references
Synthesis and characterization of membranes obtained by graft copolymerization of 2-hydroxyethyl methacrylate and acrylic acid onto chitosan.
K. C. Dos Santos (2006)
Mass spectrometry characterization of an Aloe vera mannan presenting immunostimulatory activity.
J. Simões (2012)
Acetylation and deacetylation of non-histone proteins.
M. Glozak (2005)
A validated 1H NMR method for the determination of the degree of deacetylation of chitosan.
M. Lavertu (2003)
Recognization of receptors on bone marrow-derived dendritic cells bound with Pholiota nameko polysaccharides.
H. Li (2015)
Effect of acemannan on BMP-2 expression in primary pulpal fibroblasts and periodontal fibroblasts, in vitro study
N. Jittapiromsak (2007)
HGF regulates VEGF expression via the c-Met receptor downstream pathways, PI3K/Akt, MAPK and STAT3, in CT26 murine cells.
A. Matsumura (2013)
Effect of deacetylation on the glucomannan gelation process for making restructured seafood products
Beatriz Solo-de-Zaldívar (2014)
The Acetylase/Deacetylase Couple CREB-binding Protein/Sirtuin 1 Controls Hypoxia-inducible Factor 2 Signaling*
R. Chen (2012)
Nitric oxide production by chicken macrophages activated by Acemannan, a complex carbohydrate extracted from Aloe vera.
K. Karaca (1995)
Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis
D. Shweiki (1992)
Acemannan purified from Aloe vera induces phenotypic and functional maturation of immature dendritic cells.
J. Lee (2001)
Biology of wound healing.
I. Aukhil (2000)
Role of extracellular matrix in adaptation of tendon and skeletal muscle to mechanical loading.
M. Kjaer (2004)
The role of vascular endothelial growth factor in wound healing.
Philip Bao (2009)
Loss of Vascular Endothelial Growth Factor A Activity in Murine Epidermal Keratinocytes Delays Wound Healing and Inhibits Tumor Formation
H. Rossiter (2004)
Acemannan sponges stimulate alveolar bone, cementum and periodontal ligament regeneration in a canine class II furcation defect model.
P. Chantarawaratit (2014)
A mannose-binding receptor is expressed on human keratinocytes and mediates killing of Candida albicans.
G. Szolnoky (2001)
Fluctuations in superhelical DNA.
A. Vologodskii (1979)
Acemannan stimulates gingival fibroblast proliferation; expressions of keratinocyte growth factor-1, vascular endothelial growth factor, and type I collagen; and wound healing.
Suwimon Jettanacheawchankit (2009)
Evaluation and comparison of commercially available Aloe vera L. products using size exclusion chromatography with refractive index and multi-angle laser light scattering detection.
C. Turner (2004)
Compositional features of polysaccharides from Aloe vera (Aloe barbadensis Miller) plant tissues
A. Femenia (1999)
A review on the relationship between aloe vera components and their biologic effects
S. Choi (2003)
Optimization of parameters for semiempirical methods I. Method
J. J. Stewart (1989)
Synthesis and Characterization of KonjacGlucomannan-Graft-Polyacrylamide via γ-Irradiation
Zhenlin Xu (2008)
Study on antimicrobial activity of chitosan with different molecular weights
lian-ying Zheng (2003)
Chemical characterization of the immunomodulating polysaccharide of Aloe vera L.
Jimmy Tai-Nin Chow (2005)
Supercoiling in DNA and chromatin☆
N. Gilbert (2014)
Chemical biology of peptidoglycan acetylation and deacetylation.
P. Moynihan (2014)
This paper is referenced by
Development, characterization, and biological assessment of biocompatible cellulosic wound dressing grafted Aloe vera bioactive polysaccharide
F. Salah (2019)
Bio-hybrid acrylic hydrogels containing metronidazole – loaded poly(acrylic acid-co-methyl methacrylate) nanoparticles and Aloe vera as natural healing agent
Katarzyna Bialik-Wąs (2019)
Development and characterization of reconstituted hydrogel from Aloe vera (Aloe barbadensis Miller) powder
P. Kiran (2016)
Viscoelastic behavior of reconstituted Aloe vera hydrogels as a function of concentration and temperature
P. Kiran (2017)
Polyelectrolyte complex of Aloe vera, chitosan, and alginate produced fibroblast and lymphocyte viabilities and migration.
R. Gallardo-Rivera (2018)
Application of thermosonication for Aloe vera (Aloe barbadensis Miller) juice processing: Impact on the functional properties and the main bioactive polysaccharides.
Guadalupe Alvarado-Morales (2019)
solation , purification , characterization and immunostimulatory ctivity of polysaccharides derived from American ginseng iao
- Hong Yu (2016)
Prevalencia y caracterización clínico-lesional de los principales procesos infecciosos de etiología vírica que afectan a las colonias de gatos callejeros en Madrid capital
B. Conde (2016)
Aloe Vera Extracted Polysaccharides Shift the Immune Responses of Tumor Bearing Mice Toward Th1 Pattern: Animal Study
Mohammad Hossein Yazdi (2019)
Effect of different drying procedures on the bioactive polysaccharide acemannan from Aloe vera (Aloe barbadensis Miller).
R. Minjares-Fuentes (2017)
Role of acemannan O-acetyl group in murine radioprotection.
S. Kumar (2019)
DoRWA3 from Dendrobium officinale Plays an Essential Role in Acetylation of Polysaccharides
Can Si (2020)
Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays
L. Ahl (2019)
Could We Really Use Aloe vera Food Supplements to Treat Diabetes? Quality Control Issues
S. Habtemariam (2017)
Polysaccharide from leaf skin of Aloe barbadensis Miller: Part I. Extraction, fractionation, physicochemical properties and structural characterization
Xiao-dan Shi (2017)
Influence of water deficit on the main polysaccharides and the rheological properties of Aloe vera (Aloe barbadensis Miller) mucilage
R. Minjares-Fuentes (2017)
Plant-derived glucomannans: Sources, preparation methods, structural features, and biological properties
Xiaodan Shi (2020)
Structural characterization and anti-inflammatory effect in hepatocytes of a galactoglucan from Antrodia camphorata mycelium
Huiling Tang (2019)
Nonvolatile Memory Device Based on Bipolar and Unipolar Resistive Switching in Bio‐Organic Aloe Polysaccharides Thin Film
Z. Lim (2018)
Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering
Ricardo Donate (2020)
Functional Applications of Aloe vera on Textiles: A Review
M. Mondal (2020)
Acemannan increases NF-κB/DNA binding and IL-6/-8 expression by selectively binding Toll-like receptor-5 in human gingival fibroblasts.
P. Thunyakitpisal (2017)
Flexural Strength and Cytotoxicity of ZCC, a Resin Modified Zinc-Calcium Liner Prototype
P. Thunyakitpisal (2017)
Acemannan increased bone surface, bone volume, and bone density in a calvarial defect model in skeletally-mature rats
Dyna Jeanne D. Godoy (2018)
Role of Glucomannans in Immunology.
R. Tester (2017)
Stimulation of Dentin Regeneration by Using Acemannan in Teeth with Lipopolysaccharide‐induced Pulp Inflammation
Siriporn Songsiripradubboon (2017)
Effects of UV-C irradiation and traditional thermal processing on acemannan contained in Aloe vera gel blends.
Maylem Z Rodríguez-Rodríguez (2019)
Purification, characterization and immunomodulatory activity of fructans from Polygonatum odoratum and P. cyrtonema.
P. Zhao (2019)
Aloe Vera for Tissue Engineering Applications
S. Rahman (2017)
Vitamin C Encapsulation by a Gelation Method using Deacetylated Glucomannan as a Matrix
D. H. Wardhani (2020)
Physico-chemical properties of hybrid electrospun nanofibers containing polyvinylpyrrolidone (PVP), propolis and aloe vera
A. B. Moghaddam (2018)
Comparative study on glucomannans with different structural characteristics: Functional properties and intestinal production of short chain fatty acids.
Xiao-dan Shi (2020)See more