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Effect Of Deacetylation Degree On Controlled Pilocarpine Release From Injectable Chitosan-g-poly(N-isopropylacrylamide) Carriers.

Li-Jyuan Luo, C. Huang, H. Chen, J. Lai, M. Matsusaki
Published 2018 · Chemistry, Medicine

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Development of biodegradable thermogels as intracameral injectable carriers for ocular delivery of antiglaucoma medications can provide a better treatment modality with low dosing frequency than eye drop formulations. For the first time, this study investigates the effect of deacetylation degree (DD) of the polysaccharide component in chitosan-g-poly(N-isopropylacrylamide) (CN) carriers on controlled release of pilocarpine in the management of glaucoma. Our results showed that increasing the chitosan DD from 60.7% to 98.5% leads to enhanced biodegradation resistance of carrier and prolonged release profile of the drug. Significant DNA damage and caspase-3 activation could be detected in lens epithelial cell cultures exposed to CN made from highly deacetylated polysaccharides, indicating apoptosis-related cytotoxicity due to relatively high positive charge density of the graft copolymers. Postoperative outcomes demonstrated that long-term therapeutic efficacy in glaucomatous rabbits is governed by intraocular pressure changes in response to intracamerally administered pilocarpine-loaded CN, strongly suggesting the usefulness of deacetylation in this injectable drug delivery carrier.
This paper references
10.1517/17425247.2011.581656
Thermosensitive hydrogels for drug delivery
Zhenqing Li (2011)
10.3390/ijms11125256
In Vitro Response of Retinal Pigment Epithelial Cells Exposed to Chitosan Materials Prepared with Different Cross-Linkers
J. Lai (2010)
10.1016/S0142-9612(96)00167-6
In vitro and in vivo degradation of films of chitin and its deacetylated derivatives.
K. Tomihata (1997)
10.1038/srep42344
In vivo Pharmacological Evaluations of Pilocarpine-Loaded Antioxidant-Functionalized Biodegradable Thermogels in Glaucomatous Rabbits
Shih-feng Chou (2017)
10.1002/marc.201600371
Stimuli-Sensitive Injectable Hydrogels Based on Polysaccharides and Their Biomedical Applications.
T. Thambi (2016)
10.1016/S0032-3861(97)00118-3
N.m.r. investigation of phase separation in poly(N-isopropyl acrylamide)/water solutions
F. Zeng (1997)
10.1016/j.ijbiomac.2015.03.031
Chronotherapeutic drug delivery of Tamarind gum, Chitosan and Okra gum controlled release colon targeted directly compressed Propranolol HCl matrix tablets and in-vitro evaluation.
A. Newton (2015)
10.1016/j.carbpol.2013.09.014
Water soluble folate-chitosan nanogels crosslinked by genipin.
M. A. Pujana (2014)
10.1016/J.CARRES.2005.07.022
The enzymatic degradation and swelling properties of chitosan matrices with different degrees of N-acetylation.
Dongwen Ren (2005)
10.1016/J.CARBPOL.2006.02.019
Effects of degree of deacetylation and cross-linking on physical characteristics, swelling and release behavior of chitosan microspheres
K. Gupta (2006)
10.1002/APP.12337
Dynamic swelling behavior of pH-sensitive anionic hydrogels used for protein delivery
Bumsang Kim (2003)
10.1016/S0032-3861(00)00177-4
Synthesis, characterisation and solution behaviour of thermo- and pH-responsive polymers bearing l-leucine residues in the side chains
F. Bignotti (2000)
10.2174/1381612821666150109144613
Applications of Important Polysaccharides in Drug Delivery.
Gangliang Huang (2015)
10.1002/jbm.a.34283
In vitro bioactivity of different degree of deacetylation chitosan, a potential coating material for titanium implants.
R. Lieder (2012)
10.1021/acs.biomac.5b00854
Antioxidant Gallic Acid-Functionalized Biodegradable in Situ Gelling Copolymers for Cytoprotective Antiglaucoma Drug Delivery Systems.
J. Lai (2015)
10.1016/j.ejpb.2016.11.038
Chitosan‐g‐poly(N‐isopropylacrylamide) copolymers as delivery carriers for intracameral pilocarpine administration
J. Lai (2017)
10.1111/j.1349-7006.2001.tb01116.x
Chitosan Induces Apoptosis via Caspase‐3 Activation in Bladder Tumor Cells
M. Hasegawa (2001)
10.1016/j.colsurfb.2012.11.030
In vitro biocompatibility of magnetic thermo-responsive nanohydrogel particles of poly(N-isopropylacrylamide-co-acrylic acid) with Fe3O4 cores: effect of particle size and chemical composition.
Feng-Yi Chou (2013)
10.1016/j.ijpharm.2016.06.129
On the importance of Bloom number of gelatin to the development of biodegradable in situ gelling copolymers for intracameral drug delivery.
Shih-feng Chou (2016)
10.1038/nmat3209
Polymers for gene delivery: Charged for success.
E. Mastrobattista (2011)
10.1016/j.colsurfb.2016.02.051
Relationships between surface roughness/stiffness of chitosan coatings and fabrication of corneal keratocyte spheroids: Effect of degree of deacetylation.
Shih-feng Chou (2016)
10.1016/j.msec.2017.02.118
Fabrication of thermal sensitive folic acid based supramolecular hybrid gels for injectable drug release gels.
Yahui Song (2017)
10.1016/S0142-9612(00)00326-4
In vitro degradation of chitosan by a commercial enzyme preparation: effect of molecular weight and degree of deacetylation.
H. Zhang (2001)
10.1016/J.BEJ.2006.06.014
Water permeability of chitosan membrane involved in deacetylation degree control
Tomoki Takahashi (2007)
10.1016/J.BIOMATERIALS.2003.12.036
The effect of the degree of chitosan deacetylation on the efficiency of gene transfection.
T. Kiang (2004)
10.1016/J.BIOMATERIALS.2003.10.080
A preliminary study on chitosan and gelatin polyelectrolyte complex cytocompatibility by cell cycle and apoptosis analysis.
J. Mao (2004)
10.1016/j.carbpol.2013.10.079
In situ gelling dorzolamide loaded chitosan nanoparticles for the treatment of glaucoma.
Shefali Katiyar (2014)
10.1016/J.CARBPOL.2006.07.007
Effects of molecular weight and deacetylation degree of chitin/chitosan on wound healing
Tatsuya Minagawa (2007)
10.1023/A:1008997311364
Cellular responses to chitosan in vitro: The importance of deacetylation
M. Prasitsilp (2000)
10.1016/j.carbpol.2018.03.091
Sulfated polysaccharide mediated TGF-β1 presentation in pre-formed injectable scaffolds for cartilage tissue engineering.
Neha Ashok Waghmare (2018)
10.1023/B:PHAM.0000016249.52831.a5
Uptake and Cytotoxicity of Chitosan Molecules and Nanoparticles: Effects of Molecular Weight and Degree of Deacetylation
M. Huang (2004)
10.1016/j.msec.2016.02.069
Dependence of corneal keratocyte adhesion, spreading, and integrin β1 expression on deacetylated chitosan coating.
Chi-Chin Sun (2016)
10.1016/J.FOODCHEM.2007.08.044
Hypocholesterolaemic effects of different chitosan samples in vitro and in vivo
Jingna Liu (2008)
10.1371/journal.pone.0009050
VAV2 and VAV3 as Candidate Disease Genes for Spontaneous Glaucoma in Mice and Humans
Keiko Fujikawa (2010)
10.1016/j.drudis.2007.11.002
Current and future ophthalmic drug delivery systems. A shift to the posterior segment.
Eva M. del Amo (2008)
10.3390/ijms130910970
Biocompatibility of Genipin and Glutaraldehyde Cross-Linked Chitosan Materials in the Anterior Chamber of the Eye
J. Lai (2012)
10.2217/nnm.13.148
Quinapyramine sulfate-loaded sodium alginate nanoparticles show enhanced trypanocidal activity.
A. Manuja (2014)
10.1016/j.actbio.2016.04.035
Gallic acid grafting effect on delivery performance and antiglaucoma efficacy of antioxidant-functionalized intracameral pilocarpine carriers.
Shih-feng Chou (2016)
10.1016/j.carbpol.2015.09.072
Composite chitosan hydrogels for extended release of hydrophobic drugs.
Keren Delmar (2016)
10.1016/j.carbpol.2016.02.080
Thermosensitive chitosan-based hydrogel as a topical ocular drug delivery system of latanoprost for glaucoma treatment.
Y. H. Cheng (2016)
10.1016/j.biomaterials.2011.11.085
A gelatin-g-poly(N-isopropylacrylamide) biodegradable in situ gelling delivery system for the intracameral administration of pilocarpine.
J. Lai (2012)
10.1007/S10856-007-3066-X
In vitro characterization of chitosan scaffolds: influence of composition and deacetylation degree
R. Seda Tıǧlı (2007)
10.1002/JBM.A.30421
Pulmonary inflammation caused by chitosan microparticles.
Y. Huang (2005)
10.1007/s10965-012-0032-1
Properties of poly(vinylidene fluoride)-graft-poly(N-isopropylacrylamide) membranes prepared by alkali treatment
Y. Zhao (2013)
10.1517/17425247.2012.681775
Ocular application of chitosan
E. Başaran (2012)
10.1016/j.actbio.2016.11.055
The role of alkyl chain length of monothiol-terminated alkyl carboxylic acid in the synthesis, characterization, and application of gelatin-g-poly(N-isopropylacrylamide) carriers for antiglaucoma drug delivery.
Li-Jyuan Luo (2017)
10.1038/s41598-017-09913-8
Epigallocatechin Gallate-Loaded Gelatin-g-Poly(N-Isopropylacrylamide) as a New Ophthalmic Pharmaceutical Formulation for Topical Use in the Treatment of Dry Eye Syndrome
Li-Jyuan Luo (2017)



This paper is referenced by
10.3390/ijms19113294
Effect of Cross-Linking Density on the Structures and Properties of Carbodiimide-Treated Gelatin Matrices as Limbal Stem Cell Niches
J. Lai (2018)
10.1016/j.ijbiomac.2020.04.004
Influence of galactomannan molar mass on particle size galactomannan-grafted-poly-N-isopropylacrylamide copolymers.
R. F. Gomes (2020)
10.3390/polym12030611
Solid-State Synthesis of Water-Soluble Chitosan-g-Hydroxyethyl Cellulose Copolymers
T. Demina (2020)
10.1016/j.jconrel.2019.11.038
Benzoic acid derivative-modified chitosan-g-poly(N-isopropylacrylamide): Methoxylation effects and pharmacological treatments of Glaucoma-related neurodegeneration.
Li-Jyuan Luo (2019)
10.1016/j.carbpol.2019.115770
The role of aromatic ring number in phenolic compound-conjugated chitosan injectables for sustained therapeutic antiglaucoma efficacy.
D. D. Nguyen (2020)
10.1016/j.msec.2018.11.052
Photocrosslinked hydrogels from coumarin derivatives of hyaluronic acid for tissue engineering applications.
Riccardo Beninatto (2019)
10.1016/j.ijbiomac.2019.10.120
Synthesis of magnetic gold coated poly (ε-caprolactonediol) based polyurethane/poly(N-isopropylacrylamide)-grafted-chitosan core-shell nanofibers for controlled release of paclitaxel and 5-FU.
Amirnezam Farboudi (2019)
10.1039/D0PY00919A
Advancing the stimuli response of polymer-based drug delivery systems for ocular disease treatment
D. D. Nguyen (2020)
10.1016/j.ijbiomac.2019.02.031
Chitosan based thermosensitive injectable hydrogels for controlled delivery of loxoprofen: development, characterization and in-vivo evaluation.
Umair Ahmad (2019)
10.3390/polym12071519
Chitosan and its Derivatives for Ocular Delivery Formulations: Recent Advances and Developments
Alexandra Zamboulis (2020)
10.1016/J.REACTFUNCTPOLYM.2019.04.014
Synthesis and characterization of cellulose based adsorbents for removal of Ni(II), Cu(II) and Pb(II) ions from aqueous solutions
R. Kumar (2019)
10.1016/j.xphs.2020.09.010
Comparison of different chitosan lipid nanoparticles for improved ophthalmic tetrandrine delivery: formulation, characterization, pharmacokinetic and molecular dynamics simulation.
Jinjing Li (2020)
10.3390/polym13010056
Recent Advances in Functional Polymers Containing Coumarin Chromophores
Ines Cazin (2020)
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