Online citations, reference lists, and bibliographies.
← Back to Search

Encapsulins: Microbial Nanocompartments With Applications In Biomedicine, Nanobiotechnology And Materials Science.

Tobias W. Giessen
Published 2016 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Compartmentalization is one of the defining features of life. Cells use protein compartments to exert spatial control over their metabolism, store nutrients and create unique microenvironments needed for essential physiological processes. Encapsulins are a recently discovered class of protein nanocompartments found in bacteria and archaea that naturally encapsulate cargo proteins. A short C-terminal targeting sequence directs the highly specific encapsulation process in vivo. Here, I will initially discuss the properties, diversity and putative function of encapsulins. The unique characteristics and potential uses of the self-sorting cargo-packaging process found in encapsulin systems will then be highlighted. Examples for the application of encapsulins as cell-specific optical nanoprobes and targeted therapeutic delivery systems will be discussed with an emphasis on the ability to integrate multiple functionalities within a single nanodevice. By fusing targeting sequences to non-native proteins, encapsulins can also be used as specific nanocontainers and enzymatic nanoreactors in vivo. I will end by briefly discussing future avenues for encapsulin research related to both basic microbial metabolism and applications in biomedicine, catalysis and materials science.
This paper references
10.1016/j.addr.2012.09.037
Liposomal drug delivery systems: from concept to clinical applications.
T. Allen (2013)
10.1016/j.addr.2012.01.005
Virus-based nanocarriers for drug delivery.
Y. Ma (2012)
10.1002/cbic.201100469
Cell Targeting with Hybrid Qβ Virus‐Like Particles Displaying Epidermal Growth Factor
J. Pokorski (2011)
10.1021/JA058363S
A simple tagging system for protein encapsulation.
F. Seebeck (2006)
10.1002/mrm.21761
A human ferritin iron oxide nano‐composite magnetic resonance contrast agent
M. Uchida (2008)
10.1016/j.virol.2011.06.019
Fossil record of an archaeal HK97-like provirus.
Joshua Heinemann (2011)
10.1016/j.cbpa.2014.12.026
Use of the confined spaces of apo-ferritin and virus capsids as nanoreactors for catalytic reactions.
B. Maity (2015)
10.1002/smll.200801602
Protein-based nanomedicine platforms for drug delivery.
A. Maham (2009)
10.1002/ANIE.200502113
Polymer therapeutics: concepts and applications.
R. Haag (2006)
10.1159/000351343
Eut Bacterial Microcompartments: Insights into Their Function, Structure, and Bioengineering Applications
Mark Held (2013)
10.1146/annurev-med-040210-162544
Nanoparticle delivery of cancer drugs.
A. Wang (2012)
10.1126/SCIENCE.289.5487.2129
Topologically linked protein rings in the bacteriophage HK97 capsid.
W. R. Wikoff (2000)
10.1021/nn900002m
Impact of nanotechnology on drug delivery.
O. Farokhzad (2009)
10.1073/PNAS.0502164102
Structural and functional similarities between the capsid proteins of bacteriophages T4 and HK97 point to a common ancestry.
A. Fokine (2005)
10.1128/IAI.66.6.2728-2735.1998
Identification and Characterization of a 29-Kilodalton Protein from Mycobacterium tuberculosis Culture Filtrate Recognized by Mouse Memory Effector Cells
I. Rosenkrands (1998)
10.1016/j.copbio.2012.05.004
Vault particles: a new generation of delivery nanodevices.
A. Casañas (2012)
10.1039/c3cc44508a
A nanocarrier based on a genetically engineered protein cage to deliver doxorubicin to human hepatocellular carcinoma cells.
R. Toita (2013)
10.1016/S0014-5793(98)01451-3
Homomultimeric protease in the hyperthermophilic bacterium Thermotoga maritima has structural and amino acid sequence homology to bacteriocins in mesophilic bacteria
P. Hicks (1998)
10.1111/febs.12255
The lumazine synthase/riboflavin synthase complex: shapes and functions of a highly variable enzyme system
R. Ladenstein (2013)
10.1016/j.tcb.2012.07.002
Designing biological compartmentalization.
Anna H. Chen (2012)
10.1016/J.JMB.2007.02.075
The crystal structure of a virus-like particle from the hyperthermophilic archaeon Pyrococcus furiosus provides insight into the evolution of viruses.
F. Akita (2007)
10.1007/978-1-4614-0980-9_15
Bacteriophage HK97 capsid assembly and maturation.
R. Hendrix (2012)
10.1021/JA0655690
Targeting of cancer cells with ferrimagnetic ferritin cage nanoparticles.
M. Uchida (2006)
10.1016/j.bbagen.2010.03.021
The iron redox and hydrolysis chemistry of the ferritins.
Fadi Bou-Abdallah (2010)
10.1016/j.jmb.2015.09.009
Encapsulation as a Strategy for the Design of Biological Compartmentalization.
Tobias W. Giessen (2016)
10.1128/AEM.60.10.3809-3814.1994
Isolation and characterization of Linocin M18, a bacteriocin produced by Brevibacterium linens.
N. Valdés-Stauber (1994)
10.1039/c3cs60181a
Spiropyran-based dynamic materials.
R. Klajn (2014)
10.1111/febs.12234
Assembly in vitro of Rhodococcus jostii RHA1 encapsulin and peroxidase DypB to form a nanocompartment
R. Rahmanpour (2013)
10.1021/nn4006544
Biomimetic antigenic nanoparticles elicit controlled protective immune response to influenza.
Dustin P Patterson (2013)
10.1016/J.AB.2007.10.022
Controlled antibody immobilization onto immunoanalytical platforms by synthetic peptide.
Yongwon Jung (2008)
10.1098/rstb.2009.0252
Evolutionary origins of metabolic compartmentalization in eukaryotes
W. Martin (2010)
10.1128/JVI.79.23.14967-14970.2005
Common Ancestry of Herpesviruses and Tailed DNA Bacteriophages
M. Baker (2005)
10.1002/cphc.201402722
Self-assembled cage-like protein structures.
R. M. Putri (2015)
10.1128/MMBR.00061-12
Functions, Compositions, and Evolution of the Two Types of Carboxysomes: Polyhedral Microcompartments That Facilitate CO2 Fixation in Cyanobacteria and Some Proteobacteria
Benjamin D Rae (2013)
10.1021/bm501066m
Developing genetically engineered encapsulin protein cage nanoparticles as a targeted delivery nanoplatform.
H. Moon (2014)
10.1016/S0169-409X(02)00044-3
Nanoparticles in cancer therapy and diagnosis.
I. Brigger (2002)
10.1186/2055-7124-18-21
Genetically engineering encapsulin protein cage nanoparticle as a SCC-7 cell targeting optical nanoprobe
H. Moon (2014)
10.1016/B978-0-12-415906-8.00008-X
Bacterial microcompartments insights into the structure, mechanism, and engineering applications.
S. J. Tsai (2011)
10.15252/embj.201488566
A virus capsid‐like nanocompartment that stores iron and protects bacteria from oxidative stress
C. McHugh (2014)
10.1002/smll.201301393
Fabrication of nanoarchitectures templated by virus-based nanoparticles: strategies and applications.
F. Li (2014)
10.1074/jbc.M110.160580
Structural Insight into the Mechanisms of Transport across the Salmonella enterica Pdu Microcompartment Shell*
Christopher S. Crowley (2010)
10.1016/j.ceb.2013.12.007
Compartmentalization and organelle formation in bacteria.
E. Cornejo (2014)
10.1042/BJ20120957
Evolution of intracellular compartmentalization.
Yoan Diekmann (2013)
10.1074/jbc.M114.570119
Characterization of a Mycobacterium tuberculosis Nanocompartment and Its Potential Cargo Proteins*
Heidi Contreras (2014)
10.1016/J.SBI.2005.10.012
What does structure tell us about virus evolution?
D. Bamford (2005)
10.1002/bit.25322
Packaging guest proteins into the encapsulin nanocompartment from Rhodococcus erythropolis N771
A. Tamura (2015)
10.1038/nrmicro2834
Distribution, formation and regulation of gas vesicles
F. Pfeifer (2012)
10.1021/cr100357h
Self-sorting phenomena in complex supramolecular systems.
Marina M. Safont-Sempere (2011)
10.1002/cphc.201600013
Labelling Bacterial Nanocages with Photo‐switchable Fluorophores
R. M. Putri (2016)
10.1021/cb4006529
Encapsulation of an enzyme cascade within the bacteriophage P22 virus-like particle.
Dustin P Patterson (2014)
10.1021/ja410891c
Self-sorting of foreign proteins in a bacterial nanocompartment.
W. F. Rurup (2014)
10.1038/nsmb.1473
Structural basis of enzyme encapsulation into a bacterial nanocompartment
M. Sutter (2008)
10.1002/1521-3757(20020315)114:6<1059::aid-ange1059>3.0.co;2-o
Compartmentalization of a gadolinium complex in the apoferritin cavity: a route to obtain high relaxivity contrast agents for magnetic resonance imaging.
S. Aime (2002)
10.1021/bc300015f
Development of human hepatocellular carcinoma cell-targeted protein cages.
R. Toita (2012)
10.1073/pnas.1303047110
Structure of the archaeal head-tailed virus HSTV-1 completes the HK97 fold story
M. Pietilä (2013)
10.1007/s00018-005-5285-y
Cellular regulation and molecular interactions of the ferritins
K. J. Hintze (2005)
10.1016/S0169-409X(01)00222-8
Advanced drug delivery devices via self-assembly of amphiphilic block copolymers.
A. Rösler (2001)
10.1042/BJ20060874
Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging.
Hui-wang Ai (2006)
10.1186/1475-2859-10-92
Self-assembling, protein-based intracellular bacterial organelles: emerging vehicles for encapsulating, targeting and delivering therapeutical cargoes
J. Corchero (2011)
10.1016/j.jconrel.2011.12.012
Polymeric micelles drug delivery system in oncology.
J. Gong (2012)



This paper is referenced by
10.1186/s12934-018-0873-3
Construction of an organelle-like nanodevice via supramolecular self-assembly for robust biocatalysts
H. Li (2018)
10.1042/BST20170564
Bio-engineering of bacterial microcompartments: a mini review.
S. Planamente (2019)
10.1002/JCTB.5731
Engineering substrate channeling in biosystems for improved efficiency
Tianwen Wang (2018)
10.1016/j.cell.2019.07.027
Just Solid or Liquid Enough
Lara Szewczak (2019)
10.1002/IJCH.201900013
Multi‐Component Self‐Assembly of Proteins and Inorganic Particles: From Discrete Structures to Biomimetic Materials
Matthias Künzle (2019)
10.1080/1040841X.2019.1593101
Iron/iron oxide nanoparticles: advances in microbial fabrication, mechanism study, biomedical, and environmental applications
N. Ashraf (2019)
10.1101/220293
Phospho-signal flow from a pole-localized microdomain spatially patterns transcription factor activity
Keren Lasker (2017)
10.1016/J.BPJ.2020.03.015
Diffusion of DNA-Binding Species in the Nucleus: A Transient Anomalous Subdiffusion Model.
M. Saxton (2020)
10.1021/acsnano.7b07669
Structural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptake
R. M. Putri (2017)
Biotechnology and Bioengineering
Viki R Chopda (2019)
10.1073/pnas.1621061114
Multiple origins of viral capsid proteins from cellular ancestors
M. Krupovic (2017)
10.1101/2020.05.24.113720
Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur metabolism
Robert J. Nichols (2020)
10.1021/acs.nanolett.8b02991
In Vitro Assembly of Diverse Bacterial Microcompartment Shell Architectures.
A. Hagen (2018)
10.1016/j.copbio.2017.03.018
Bioengineering towards self-assembly of particulate vaccines.
B. H. Rehm (2017)
10.1016/j.cbpa.2017.03.009
Iron assimilation and utilization in anaerobic ammonium oxidizing bacteria.
C. Ferousi (2017)
10.1016/j.cbpa.2018.11.021
Advances and opportunities for the design of self-sufficient and spatially organized cell-free biocatalytic systems.
Claudia Schmid-Dannert (2019)
10.1080/10409238.2017.1337709
Encapsulins: molecular biology of the shell
Robert J. Nichols (2017)
10.1002/asia.201800842
Self-Assembly and Stabilization of Hybrid Cowpea Chlorotic Mottle Virus Particles under Nearly Physiological Conditions.
S. P. Timmermans (2018)
10.1016/j.copbio.2017.01.004
Engineering carbon fixation with artificial protein organelles.
Tobias W. Giessen (2017)
10.1007/978-1-4939-7893-9_2
Encapsulation of Active Enzymes within Bacteriophage P22 Virus-Like Particles.
Dustin P Patterson (2018)
10.1007/978-981-13-0445-3_2
Protein Cage Nanoparticles as Delivery Nanoplatforms.
Bongseo Choi (2018)
10.1038/nmicrobiol.2017.29
Widespread distribution of encapsulin nanocompartments reveals functional diversity
Tobias W. Giessen (2017)
10.1186/s12915-020-0751-4
Protein assembly systems in natural and synthetic biology
Giulio Chiesa (2020)
10.1016/j.vaccine.2018.05.034
Simultaneous surface display and cargo loading of encapsulin nanocompartments and their use for rational vaccine design.
P. Lagoutte (2018)
10.1002/cbic.201600431
A Catalytic Nanoreactor Based on in Vivo Encapsulation of Multiple Enzymes in an Engineered Protein Nanocompartment
Tobias W. Giessen (2016)
10.1101/222083
Eukaryotically expressed encapsulins as orthogonal compartments for multiscale molecular imaging
Felix Sigmund (2017)
10.3390/genes9070370
Bioengineering Strategies for Protein-Based Nanoparticles
D. Diaz (2018)
10.1016/j.actbio.2020.03.020
Advancements in prophylactic and therapeutic nanovaccines
P. Bhardwaj (2020)
Nanoparticles based on encapsuline.
Priscilla Lagoutte (2016)
10.1101/742171
Diffusion of DNA-binding species in the nucleus: A transient anomalous subdiffusion model
M. Saxton (2019)
10.1016/j.cell.2018.01.014
Subcellular Organization: A Critical Feature of Bacterial Cell Replication
I. Surovtsev (2018)
New insights into synthesis and function of heme c proteins of anammox bacteria
C. Ferousi (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar