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Expanded Separation Technique For Chlorophyll Metabolites In Oriental Tobacco Leaf Using Non Aqueous Reversed Phase Chromatography.

N. Ishida
Published 2011 · Chemistry, Medicine

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An improved separation method for chlorophyll metabolites in Oriental tobacco leaf was developed. While Oriental leaf still gives the green color even after the curing process, little attention has been paid to the detailed composition of the remaining green pigments. This study aimed to identify the green pigments using non aqueous reversed phase chromatography (NARPC). To this end, liquid chromatograph (LC) equipped with a photo diode array detector (DAD) and an atmospheric pressure chemical ionization/mass spectrometer (APCI/MSD) was selected, because it is useful for detecting low polar non-volatile compounds giving green color such as pheophytin a. Identification was based on the wavelength spectrum, mass spectrum and retention time, comparing the analytes in Oriental leaf with the commercially available and synthesized components. Consequently, several chlorophyll metabolites such as hydroxypheophytin a, solanesyl pheophorbide a and solanesyl hydroxypheophorbide a were newly identified, in addition to typical green pigments such as chlorophyll a and pheophytin a. Chlorophyll metabolites bound to solanesol were considered the tobacco specific components. NARPC expanded the number of detectable low polar chlorophyll metabolites in Oriental tobacco leaf.
This paper references
10.1016/S0021-9673(01)00663-X
Development and application of a high resolution liquid chromatographic method for the analysis of complex pigment distributions.
R. L. Airs (2001)
10.1016/S0968-0896(01)00305-4
Antitumor agents. Part 209: Pheophorbide-a derivatives as photo-Independent cytotoxic agents.
P. Wongsinkongman (2002)
10.1515/9783111419787-003
H
Yu-Qin Cao (1824)
10.1021/JF000384C
Improved method to track chlorophyll degradation.
A. Gauthier-Jaques (2001)
10.1016/J.CHROMA.2007.01.009
Separation of solanesol in tobacco leaves extract by slow rotary counter-current chromatography using a novel non-aqueous two-phase solvent system.
Yang Zhao (2007)
10.1016/j.jpba.2008.05.009
Determination of chlorophylls and their derivatives in Gynostemma pentaphyllum Makino by liquid chromatography-mass spectrometry.
S. Huang (2008)
10.1016/B978-1-4832-3289-8.50009-6
The Structure and Chemistry of Functional Groups
G. Seely (1966)
10.1016/S0021-9673(00)92332-X
Non-aqueous reversed-phase liquid chromatography
N. Parris (1978)
10.1016/S0021-9673(01)93478-8
Detection of olive oil adulteration with linoleic acid-rich oils by reversed-phase high-performance liquid chromatography.
V. Kapoulas (1986)
10.1016/S0065-2296(01)35003-6
Recent advances in the cell biology of chlorophyll catabolism
H. Thomas (2001)
10.1016/S1360-1385(00)01735-0
Degradation pathway(s) of chlorophyll: what has gene cloning revealed?
K. Takamiya (2000)
10.1021/JF00008A018
Identification of chlorophyll derivatives by mass spectrometry
R. Breemen (1991)
Chem
W. M. Manning (1943)
10.1016/0021-9673(91)85001-V
Effect of the sample solvent on band profiles in preparative liquid chromatography using non-aqueous reversed-phase high-performance liquid chromatography
P. Jandera (1991)
10.1021/JO951854I
Stereoselective Synthesis of New Chlorophyll a Related Antioxidants Isolated from Marine Organisms
Lifu Ma (1996)
10.1515/9783111576855-015
J
Seguin Hen (1824)
10.1021/AR970225N
Solving the Riddle of Chlorophyll Breakdown
B. Kraeutler (1999)
10.1021/JF030158D
Identification of chlorophylls and carotenoids in major teas by high-performance liquid chromatography with photodiode array detection.
Yasuyo Suzuki (2003)
10.1021/JA01599A041
Flue-cured Tobacco. I. Isolation of Solanesol, an Unsaturated Alcohol
R. L. Rowland (1956)
Trends Food Sci
J. W. Heaton (1996)
10.1021/JF048918N
Triacylglycerol composition of walnut (Juglans regia L.) cultivars: characterization by HPLC-ELSD and chemometrics.
J. Amaral (2004)
10.1515/9783111413426-013
L
Il Liceo (1824)
10.1007/BF02636347
Detection of olive oil adulteration with canola oil from triacylglycerol analysis by reversed-phase high-performance liquid chromatography
E. Salivaras (1992)
10.1016/J.JPBA.2006.09.003
Rapid determination of total solanesol in tobacco leaf by ultrasound-assisted extraction with RP-HPLC and ESI-TOF/MS.
J. Chen (2007)
CHLOROPHYLL D, A GREEN PIGMENT OF RED ALGAE
W. M. Manning (1943)
10.1016/J.JPBA.2007.01.021
Rapid and quantitative determination of solanesol in Nicotiana tabacum by liquid chromatography-tandem mass spectrometry.
C. Zhao (2007)
Tobacco : production, chemistry, and technology
D. L. Davis (1999)
10.1016/0924-2244(96)81352-5
Chlorophyll degradation in processed foods and senescent plant tissues
James W. Heaton (1996)
10.1104/PP.22.1.34
Changes in the Chlorophyll and Carotene Contents of Curing Burley Tobacco Cut at Different Stages of Maturity.
R. Jeffrey (1947)
10.1021/JF00065A028
Changes in chemical composition of tobacco lamina during senescence and curing. 1. Plastid pigments
H. R. Burton (1985)
Trends Plant Sci
K. Takamiya (2000)
10.1016/J.JPBA.2007.10.014
Continuous counter current extraction, isolation and determination of solanesol in Nicotiana tobacum L. by non-aqueous reversed phase high performance liquid chromatography.
R. N. Rao (2008)
10.1016/S0021-9673(99)00999-1
High-performance liquid chromatographic screening of chlorophyll derivatives produced during fruit storage.
L. Almela (2000)
10.1126/science.1191127
A Red-Shifted Chlorophyll
M. Chen (2010)
10.1080/01483919108049603
Separation of Epimeric Pairs of C-24 Alkylsterols by Reversed-Phase High Performance Liquid Chromatography of the Free Sterols at Subambient Temperature
D. Chitwood (1991)
10.1007/s000180050434
Chlorophyll breakdown in higher plants and algae
S. Hörtensteiner (1999)
10.1016/J.CHROMA.2006.07.083
Microwave-assisted extraction of solanesol from tobacco leaves.
Hua-Ying Zhou (2006)
The Porphyrins Volume III Physical Chemistry Part A
D. Dolphin (1978)



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