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Enrichment Of Circulating Tumor Cells Using A Centrifugal Affinity Plate System.

S. Lee, Kyung-A Hyun, S. Kim, Ji-Yoon Kang, H. Jung
Published 2014 · Chemistry, Medicine

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Circulating tumor cells (CTCs) are defined as cells that have detached from a primary tumor and are circulating in the bloodstream. Their isolation and quantification is of great value for cancer prognoses and drug testing. Here, the development of a centrifugal affinity plate (CAP) system is described, in which centrifugal force and antibody-based capture are exploited to enrich CTCs on one plate and hematological cells on the other. The CAP is rotated to exert centrifugal force on the cells in a blood sample, quickly transporting them to the anti-epithelial adhesion molecule (EpCAM)-coated and anti-CD45-coated surface of the CAP to shorten the reaction time and increase the adhesion force between the tumor and blood cells and each antibody. The effect of a rotating process on cell capture was investigated, and the capture efficiency was demonstrated using blood samples from healthy donors spiked with human non-small cell lung cancer (NCI-H1650) and breast cancer (MCF-7) cells. The CAP system was capable of rapid isolation and identification of CTCs without the requirement for pretreatment of blood samples. Finally, the CAP system was tested to evaluate the detection efficiency of CTCs in the blood samples of breast cancer patients. The number of captured CTCs in only 1ml of blood varied from 6 to 10.
This paper references
10.1039/c3lc50582k
Advances and critical concerns with the microfluidic enrichments of circulating tumor cells.
Kyung-A Hyun (2014)
10.1039/b917959c
Capture of circulating tumor cells from whole blood of prostate cancer patients using geometrically enhanced differential immunocapture (GEDI) and a prostate-specific antibody.
J. Gleghorn (2010)
10.1039/c0lc00130a
Adhesion based detection, sorting and enrichment of cells in microfluidic Lab-on-Chip devices.
Tohid F Didar (2010)
10.1371/journal.pone.0015624
HER2-Positive Circulating Tumor Cells in Breast Cancer
M. Ignatiadis (2011)
10.1039/c0lc00345j
Continuous separation of breast cancer cells from blood samples using multi-orifice flow fractionation (MOFF) and dielectrophoresis (DEP).
Hui-Sung Moon (2011)
10.1038/sj.bjc.6602310
Cellular and complement-dependent cytotoxicity of Ep-CAM-specific monoclonal antibody MT201 against breast cancer cell lines
N. Prang (2005)
10.1002/SMLL.201202317
A Trachea-Inspired Bifurcated Microfilter Capturing Viable Circulating Tumor Cells via Altered Biophysical Properties as Measured by Atomic Force Microscopy
김승일 (2013)
10.1039/c2lc40065k
SSA-MOA: a novel CTC isolation platform using selective size amplification (SSA) and a multi-obstacle architecture (MOA) filter.
M. Kim (2012)
10.1039/c005178k
Rapid isolation and detection of cancer cells by utilizing integrated microfluidic systems.
K. Lien (2010)
10.1002/BIT.20556
Enrichment using antibody-coated microfluidic chambers in shear flow: model mixtures of human lymphocytes.
Aaron Sin (2005)
10.1007/s10544-012-9718-8
Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system
Y. Huang (2013)
10.1002/smll.201202317
A trachea-inspired bifurcated microfilter capturing viable circulating tumor cells via altered biophysical properties as measured by atomic force microscopy.
M. Kim (2013)
10.1001/jama.2010.292
Circulating tumor cells revisited.
M. Cristofanilli (2010)
10.1158/1078-0432.CCR-04-1110
Circulating Tumor Cells in Patients with Breast Cancer Dormancy
S. Meng (2004)
10.1002/elps.201200417
Microfluidic devices for the isolation of circulating rare cells: A focus on affinity‐based, dielectrophoresis, and hydrophoresis
Kyung-A Hyun (2013)
10.1056/NEJMOA040766
Circulating tumor cells, disease progression, and survival in metastatic breast cancer.
M. Cristofanilli (2004)



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