Online citations, reference lists, and bibliographies.
← Back to Search

RGD-modified PH-sensitive Liposomes For Docetaxel Tumor Targeting.

Minglu Chang, Shanshan Lu, Fang Zhang, Tiantian Zuo, Yuanyuan Guan, T. Wei, W. Shao, Guimei Lin
Published 2015 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Phosphatidylethanolamine-based pH-sensitive liposomes of various compositions have been described as efficient systems for delivery of therapeutic molecules into tumor cells. The aim of this work was to develop a drug delivery system based on pH-sensitive liposomes (PLPs) that were modified with arginine-glycine-aspartic acid (RGD) peptide to enhance the effectiveness of docetaxel treatment. Docetaxel/coumarin-6 loaded PLPs were prepared by the thin-film dispersion method and characterized in detail, including by particle size, polydispersity, zeta potential and drug encapsulation efficiency. In vitro studies using MCF-7, HepG2and A549 cells were employed to investigate cytotoxicity and cellular uptake of the drug solution or docetaxel/coumarin-6 loaded PLPs. The accumulation of 7-nitro-2-1,3-benzoxadiazol-4-yl (NBD)-labeled liposomes in vivo was studied through tumor section imaging of xenograft mouse models of MCF-7 24h after intravenous administration. The particle size of the non-coated or RGD modified PLPs ranged between 146 and 129nm. Drug release in vitro was modestly prolonged and had good pH sensitivity. In the in vitro study, RGD-coated PLPs showed higher cytotoxicity and cellular uptake relative to non-coated ones. The results of the in vivo study showed that RGD-coated PLPs had higher fluorescence, which suggested a more efficient accumulation than normal PLPs in tumors. In conclusion, these results confirmed RGD-modified PLPs as a potential drug delivery system to achieve controlled release and tumor targeting.
This paper references
Folate-targeted therapies for cancer.
W. Xia (2010)
Cell sensitivity assays: the MTT assay.
Johan van Meerloo (2011)
Synthesis, characterization and antitumor evaluation of CMCS-DTX conjugates as novel delivery platform for docetaxel.
Fengxi Liu (2013)
Advantages of RGD peptides for directing cell association with biomaterials.
S. Bellis (2011)
Docetaxel for treatment of solid tumours: a systematic review of clinical data.
A. Montero (2005)
Cancer nanomedicines targeting tumor extracellular pH.
L. Tian (2012)
Cholesteryl hemisuccinate as a membrane stabilizer in dipalmitoylphosphatidylcholine liposomes containing saikosaponin-d.
W. Ding (2005)
A liposome-based antigen delivery system using pH-sensitive fusogenic polymers for cancer immunotherapy.
E. Yuba (2013)
Dual-functional liposomes based on pH-responsive cell-penetrating peptide and hyaluronic acid for tumor-targeted anticancer drug delivery.
Tianyue Jiang (2012)
The use of a tumor metastasis targeting peptide to deliver doxorubicin-containing liposomes to highly metastatic cancer.
Z. Wang (2012)
Antibody derivatization and conjugation strategies: application in preparation of stealth immunoliposome to target chemotherapeutics to tumor.
A. S. Manjappa (2011)
Modeling of human tumor xenografts and dose rationale in oncology.
M. Simeoni (2013)
Polymer conjugates: nanosized medicines for treating cancer.
M. Vicent (2006)
Evaluation of pH-responsive liposomes containing amino acid-based zwitterionic lipids for improving intracellular drug delivery in vitro and in vivo.
Y. Obata (2010)
Fusion-relevant changes in lipid shape of hydrated cholesterol hemisuccinate induced by pH and counterion species.
Benjamin Klasczyk (2010)
MITO-Porter: A liposome-based carrier system for delivery of macromolecules into mitochondria via membrane fusion.
Y. Yamada (2008)
pH-responsive polymer-liposomes for intracellular drug delivery and tumor extracellular matrix switched-on targeted cancer therapy.
Yi-Ting Chiang (2014)
Chemotherapeutic engineering: Application and further development of chemical engineering principles for chemotherapy of cancer and other diseases
S. Feng (2003)
Vitamin E TPGS coated liposomes enhanced cellular uptake and cytotoxicity of docetaxel in brain cancer cells.
M. Muthu (2011)
The pH-dependent association with cancer cells of tunable functionalized lipid vesicles with encapsulated doxorubicin for high cell-kill selectivity.
S. Karve (2010)
pH-sensitive vesicles, polymeric micelles, and nanospheres prepared with polycarboxylates.
Arnaud E Felber (2012)
Docetaxel-related side effects and their management.
J. Baker (2009)
Cholesteryl hemisuccinate exhibits pH sensitive polymorphic phase behavior.
I. M. Hafez (2000)
Dual Probe with Fluorescent and Magnetic Properties for Imaging Solid Tumor Xenografts
L. Shan (2007)
Hepatocellular carcinoma targeting effect of PEGylated liposomes modified with lactoferrin.
Minyan Wei (2012)
Recent advances with liposomes as pharmaceutical carriers
V. Torchilin (2005)
Potential dopamine prodrug-loaded liposomes: preparation, characterization, and in vitro stability studies
M. Carafa (2010)
Dual-targeting daunorubicin liposomes improve the therapeutic efficacy of brain glioma in animals.
X. Ying (2010)

This paper is referenced by
Recent advances on thermosensitive and pH-sensitive liposomes employed in controlled release.
Marjan Abri Aghdam (2019)
RGD-decorated solid lipid nanoparticles enhanced tumor targeting, penetration and anticancer effect of asiatic acid.
Tanem Garanti (2020)
Modulating angiogenesis with integrin‐targeted nanomedicines☆
A. Duro-Castano (2017)
Preparation, Characterization, and In Vitro pH-sensitivity Evaluation of Superparamagnetic Iron Oxide Nanoparticle- Misonidazole pH-sensitive Liposomes.
Bibo Li (2019)
RGD-Targeted Liposome Binding and Uptake on Breast Cancer Cells Is Dependent on Elastin Linker Secondary Structure.
Eleftheria Veneti (2016)
Preparation and properties evaluation of a novel pH-sensitive liposomes based on imidazole-modified cholesterol derivatives.
Liang Ju (2017)
Vitamin E D‐alpha‐tocopheryl polyethylene glycol 1000 succinate‐conjugated liposomal docetaxel reverses multidrug resistance in breast cancer cells
N. Li (2019)
A Critical Review of Properties and Analytical Methods for the Determination of Oxytetracyline in Biological and Pharmaceutical Matrices
R. Sversut (2017)
Preparation and properties evaluation of a novel pH-sensitive liposomes based on imidazole-modi fi ed cholesterol derivatives
Liang Jua (2017)
Effect of PI3K/Akt signal pathway inhibitor on docetaxel nanoparticles in cell uptake, cell colony, caspase-3 & caspase-9 expression and pharmacokinetics
Yunzhi Yin (2018)
An injectable liposome for sustained release of icariin to the treatment of acute blunt muscle injury.
Jinwu Wang (2020)
Gold Nanoframeworks with Mesopores for Raman–Photoacoustic Imaging and Photo‐Chemo Tumor Therapy in the Second Near‐Infrared Biowindow
Jinping Wang (2020)
Study of the pH-sensitive mechanism of tumor-targeting liposomes.
Y. Fan (2017)
Construction and cellular uptake behavior of redox-sensitive docetaxel prodrug-loaded liposomes
Guolian Ren (2018)
Endogenous Stimuli-Responsive Linkers in Nanoliposomal Systems for Cancer Drug Targeting.
Mahdi Faal Maleki (2019)
Stimulus-responsive liposomes as smart nanoplatforms for drug delivery applications
P. S. Zangabad (2018)
Docetaxel–trastuzumab stealth immunoliposome: development and in vitro proof of concept studies in breast cancer
A. Rodallec (2018)
Stimuli-responsive liposomes for drug delivery.
Y. Lee (2017)
Optimization of Docetaxel Loading Conditions in Liposomes: proposing potential products for metastatic breast carcinoma chemotherapy
R. Vakili-Ghartavol (2020)
Recent progress in biomedical applications of RGD-Based ligand: From precise cancer theranostics to biomaterial engineering: A systematic review.
M. Alipour (2019)
Bone-targeting drug delivery system of biomineral-binding liposomes loaded with icariin enhances the treatment for osteoporosis
Xiaoduan Sun (2019)
Dual drug-loaded nano-platform for targeted cancer therapy: toward clinical therapeutic efficacy of multifunctionality
Zhe Ma (2020)
Preparation of Drug Liposomes by Thin-Film Hydration and Homogenization
B. Xiang (2018)
Targeted delivery of anticancer drugs: new trends in lipid nanocarriers
M. B. Oliveira (2017)
A targeted nanoplatform co-delivering chemotherapeutic and antiangiogenic drugs as a tool to reverse multidrug resistance in breast cancer.
F. Tian (2018)
Membrane-Loaded Doxorubicin Liposomes Based on Ion-Pairing Technology with High Drug Loading and pH-Responsive Property
H. Xu (2016)
pH- and temperature-responsive nanosystems
M. Shah (2017)
Folate grafted thiolated chitosan enveloped nanoliposomes with enhanced oral bioavailability and anticancer activity of docetaxel.
M. Sohail (2016)
RGD(Arg-Gly-Asp) internalized docetaxel-loaded pH sensitive liposomes: Preparation, characterization and antitumor efficacy in vivo and in vitro.
Tiantian Zuo (2016)
NGR-modified pH-sensitive liposomes for controlled release and tumor target delivery of docetaxel.
Zili Gu (2017)
A novel turn off fluorescent sensor for Fe(III) and pH environment based on coumarin derivatives: the fluorescence characteristics and theoretical study
Chao-jun Hua (2016)
Preparation and in vivo safety evaluations of antileukemic homoharringtonine-loaded PEGylated liposomes
Dong Liu (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar