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Hepatocellular Carcinoma Targeting Effect Of PEGylated Liposomes Modified With Lactoferrin.
Minyan Wei, Yuehong Xu, Qi Zou, Liuxiao Tu, Chenyi Tang, T. Xu, Lihe Deng, C. Wu
Published 2012 · Chemistry, Medicine
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A hepatocellular carcinoma targeting lactoferrin (Lf) modified PEGylated liposome system was developed for improving drug efficacies to hepatic cancer cells. In this present work, PEGylated liposomes (PLS) were successfully prepared by the thin film hydration method combined with peglipid post insertion. Lf was covalently conjugated to the distal end of DSPE-PEG2000-COOH lipid by amide bound and loaded onto PEGylated liposomes surface as the targeting ligand. To confirm the targeting efficacies to hepatic cancer, coumarin-6 and DiR were encapsulated as fluorescent probes. The confocal microscopy and flow cytometry demonstrated that Lf conjugated PEGylated liposomes (Lf-PLS) were efficiently associated by HepG2 cells, while limited interaction was found for liposomes modified with a negative control protein. A similar pharmacokinetic behavior was observed in pharmacokinetics study of the liposomal formulations. Meanwhile, the in vivo imaging of liposomes in HepG2 tumor bearing mice indicated that Lf-PLS achieved more accumulation in tumor compared with PLS without Lf conjugated. The significant in vitro and in vivo results suggested that Lf-PLS might be a promising drug delivery system for hepatocellular carcinoma therapy with low toxicity.
This paper references
Mammalian lactoferrin receptors: structure and function.
Y. Suzuki (2005)
Lactoferrin and host defense.
P. Ward (2002)
Nano- and microparticles for the delivery of polypeptides and proteins
P. Couvreur (1993)
Development of pegylated liposomal vinorelbine formulation using "post-insertion" technology.
Chun Lei Li (2010)
Chloride channel-mediated brain glioma targeting of chlorotoxin-modified doxorubicine-loaded liposomes.
Y. Xiang (2011)
In vitro toxicity evaluation of single walled carbon nanotubes on human A549 lung cells.
M. Davoren (2007)
Assessing transferrin modification of liposomes by atomic force microscopy and transmission electron microscopy.
S. Anabousi (2005)
Insertion of poly(ethylene glycol) derivatized phospholipid into pre‐formed liposomes results in prolonged in vivo circulation time
P. S. Uster (1996)
Lactosaminated human albumin, a hepatotropic carrier of drugs.
L. Fiume (2010)
Lactoferrin: a multi-tasking protein par excellence.
E. Baker (2005)
Anti-CD8 conjugated nanoparticles to target mammalian cells expressing CD8.
A. Bicho (2010)
Sonosensitive dioleoylphosphatidylethanolamine-containing liposomes with prolonged blood circulation time of doxorubicin.
T. J. Evjen (2011)
Plasma clearance and liver uptake of chylomicron remnants generated by hepatic lipase lipolysis: evidence for a lactoferrin-sensitive and apolipoprotein E-independent pathway.
S. Crawford (1999)
Efficient gene silencing in metastatic tumor by siRNA formulated in surface-modified nanoparticles.
Shyh-Dar Li (2008)
Lactoferrin binding to the rat asialoglycoprotein receptor requires the receptor's lectin properties.
D. Mcabee (2000)
A combinatorial approach to producing sterically stabilized (Stealth) immunoliposomal drugs
T. Ishida (1999)
Anti-HER2 immunoliposomes: enhanced efficacy attributable to targeted delivery.
J. Park (2002)
The characteristics and performance of a multifunctional nanoassembly system for the co-delivery of docetaxel and iSur-pDNA in a mouse hepatocellular carcinoma model.
Zhenghong Xu (2010)
Identification and isolation of a 45-kDa calcium-dependent lactoferrin receptor from rat hepatocytes.
D. J. Bennatt (1997)
pH-sensitive immunoliposomes specific to the CD33 cell surface antigen of leukemic cells.
P. Simard (2009)
Lactoferrin-modified procationic liposomes as a novel drug carrier for brain delivery.
H. Chen (2010)
Lactoferrin-conjugated superparamagnetic iron oxide nanoparticles as a specific MRI contrast agent for detection of brain glioma in vivo.
H. Xie (2011)
Removal of lactoferrin from plasma is mediated by binding to low density lipoprotein receptor‐related protein/α 2‐macroglobulin receptor and transport to endosomes
M. Meilinger (1995)
Identification and Analysis of a Ca2+-Dependent Lactoferrin Receptor in Rat Liver
D. Mcabee (1998)
Up-regulation of hepatic low-density lipoprotein receptor-related protein 1: a possible novel mechanism of antiatherogenic activity of hydroxymethylglutaryl-coenzyme A reductase inhibitor Atorvastatin and hepatic LRP1 expression.
J. H. Moon (2011)
Identification and analysis of a CA(2+)-dependent lactoferrin receptor in rat liver. Lactoferrin binds to the asialoglycoprotein receptor in a galactose-independent manner.
D. Mcabee (1998)
Disaccharide-modified liposomes and their in vitro intracellular uptake.
C. K. Song (2009)
Protein-liposome conjugates using cysteine-lipids and native chemical ligation.
Sanne W. A. Reulen (2007)
Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles.
C. Kirchner (2005)
Early experience with novel immunomodulators for cancer treatment
Z. Thotathil (2007)
Nanoparticles evading the reticuloendothelial system: role of the supported bilayer.
Shyh-Dar Li (2009)
A synthetic peptide mediated active targeting of cisplatin liposomes to Tie2 expressing cells.
J. Mai (2009)
Advanced strategies in liposomal cancer therapy: problems and prospects of active and tumor specific drug release.
T. Andresen (2005)
Biophysical Aspects of Using Liposomes as Delivery Vehicles
A. Ulrich (2002)
In vitro and in vivo comparison of immunoliposomes made by conventional coupling techniques with those made by a new post-insertion approach.
D. Iden (2001)
Active targeting schemes for nanoparticle systems in cancer therapeutics.
J. D. Byrne (2008)
Oxaliplatin targeting to angiogenic vessels by PEGylated cationic liposomes suppresses the angiogenesis in a dorsal air sac mouse model.
A. Abu-Lila (2009)
Targeted delivery of doxorubicin using stealth liposomes modified with transferrin.
X. Li (2009)
Tumor cell targeting of liposome-entrapped drugs with phospholipid-anchored folic acid-PEG conjugates.
A. Gabizon (2004)
The use of lactoferrin as a ligand for targeting the polyamidoamine-based gene delivery system to the brain.
R. Huang (2008)
Diverse roles for the LDL receptor family
D. Strickland (2002)
Inhibition of liver metastasis by targeting of immunomodulators using mannosylated liposome carriers.
P. Opanasopit (2002)
Folate receptor mediated targeted delivery of ricin entrapped into sterically stabilized liposomes to human epidermoid carcinoma (KB) cells: effect of monensin intercalated into folate-tagged liposomes.
N. Tyagi (2011)
Polyethylene sebacate-doxorubicin nanoparticles for hepatic targeting.
Swati A. Guhagarkar (2010)
Systemic Therapy for Hepatocellular Carcinoma: Cytotoxic Chemotherapy, Targeted Therapy and Immunotherapy
M. Thomas (2007)
Isolated rat hepatocytes bind lactoferrins by the RHL-1 subunit of the asialoglycoprotein receptor in a galactose-independent manner.
D. J. Bennatt (1997)
Tumor targeting using anti-her2 immunoliposomes.
J. Park (2001)
Anti-neovascular therapy by use of tumor neovasculature-targeted long-circulating liposome.
N. Maeda (2004)
Tumor targeted quantum dot-mucin 1 aptamer-doxorubicin conjugate for imaging and treatment of cancer.
Ronak Savla (2011)
Nano-vectors for efficient liver specific gene transfer
A. Pathak (2008)
Morphology, binding behavior and MR-properties of paramagnetic collagen-binding liposomes.
H. M. H. F. Sanders (2009)
Targeting glioma cells in vitro with ascorbate-conjugated pharmaceutical nanocarriers.
S. Salmaso (2009)
Drug Delivery Systems: Entering the Mainstream
T. Allen (2004)
Cationic liposomes as carriers for aerosolized formulations of an anionic drug: safety and efficacy study.
Shuhua Bai (2009)
Dual-targeting daunorubicin liposomes improve the therapeutic efficacy of brain glioma in animals.
X. Ying (2010)
Cell transfection in vitro and in vivo with nontoxic TAT peptide-liposome–DNA complexes
V. Torchilin (2003)
Combined targeting of perivascular and endothelial tumor cells enhances anti-tumor efficacy of liposomal chemotherapy in neuroblastoma.
M. Loi (2010)
Tumor-targeted PE38KDEL delivery via PEGylated anti-HER2 immunoliposomes.
Jie Gao (2009)
Treatment of hepatocellular carcinoma: is there an optimal strategy?
M. Varela (2003)
Enhanced cytotoxicity of monoclonal anticancer antibody 2C5-modified doxorubicin-loaded PEGylated liposomes against various tumor cell lines.
T. Elbayoumi (2007)
In vitro and cell uptake studies for targeting of ligand anchored nanoparticles for colon tumors.
A. Jain (2008)
Lactoferrin as a gene delivery vehicle to hepatocytes
S. T. Oh (1997)
Binding and endocytosis of apo- and holo-lactoferrin by isolated rat hepatocytes.
D. McAbee (1991)
In vitro assessment of transferrin-conjugated liposomes as drug delivery systems for inhalation therapy of lung cancer.
S. Anabousi (2006)
Lactoferrin-conjugated PEG-PLA nanoparticles with improved brain delivery: in vitro and in vivo evaluations.
Kai-li Hu (2009)
The performance of docetaxel-loaded solid lipid nanoparticles targeted to hepatocellular carcinoma.
Zhenghong Xu (2009)
Lactoferrin induces cell surface retention of prion protein and inhibits prion accumulation
Y. Iwamaru (2008)
X-ray crystallographic characterization of nilvadipine monohydrate and its phase transition behavior.
F. Hirayama (2000)
Liposomal, Nanoparticle, and Conjugated Formulations of Anticancer Agents
W. Zamboni (2005)
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Nanomedicine in management of hepatocellular carcinoma: Challenges and opportunities
N. Mohamed (2017)
Enhanced therapeutic efficacy of LHRHa-targeted brucea javanica oil liposomes for ovarian cancer
Hongxia Ye (2016)
Design and evaluation of pH-sensitive liposomes constructed by poly(2-ethyl-2-oxazoline)-cholesterol hemisuccinate for doxorubicin delivery.
H. Xu (2015)
RGD-modified pH-sensitive liposomes for docetaxel tumor targeting.
Minglu Chang (2015)
Optimization of lipase-catalyzed synthesis of novel galactosyl ligands for selective targeting of liposomes to the asialoglycoprotein receptor
H. Nie (2015)
Characterization of 9-nitrocamptothecin-in-cyclodextrin-in-liposomes modified with transferrin for the treating of tumor.
J. Chen (2015)
Protein entrapment in PEGylated lipid nanoparticles.
A. Angelova (2013)
Lipase-catalyzed synthesis of novel galactosylated cholesterol
H. Nie (2015)
Nanosized systems for efficient delivery of antitumoral and anti-inflammatory drugs
R. Pireddu (2015)
Protein‐lipid nanohybrids as emerging platforms for drug and gene delivery: Challenges and outcomes
Mohamed I. Gaber (2017)
Engineered peptides for the development of actively tumor targeted liposomal carriers of doxorubicin.
M. Shahin (2013)
Novel drug delivery liposomes targeted with a fully human anti-VEGF165 monoclonal antibody show superior antitumor efficacy in vivo.
Chenyang Shi (2015)
Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
Y. Kuo (2017)
Engineering of pectin-capped gold nanoparticles for delivery of doxorubicin to hepatocarcinoma cells: an insight into mechanism of cellular uptake
Shaivee Borker (2018)
Tumor regression following intravenous administration of lactoferrin- and lactoferricin-bearing dendriplexes
Li Ying Lim (2015)
CXCR 4-targeted liposomal mediated co-delivery of pirfenidone and AMD 3100 for the treatment of TGF β-induced HSC-T 6 cells activation
Aftab Ullah (2019)
In Vitro and In Vivo Evaluation of SP94 Modified Liposomes Loaded with N-14NCTDA, a Norcantharimide Derivative for Hepatocellular Carcinoma-Targeting.
Y. Jiang (2020)
Lactoferrin Enhanced Apoptosis and Protected Against Thioacetamide-Induced Liver Fibrosis in Rats
A. Hessin (2015)
Surface Engineering of Nanoparticles for Targeted Delivery to Hepatocellular Carcinoma.
Jingxiong Lu (2018)
Lactoferrin coated or conjugated nanomaterials as an active targeting approach in nanomedicine.
Mona M. Agwa (2020)
Delivery of fluorescent-labeled cyclodextrin by liposomes: role of transferrin modification and phosphatidylcholine composition
J. Chen (2017)
Receptor-based targeting of therapeutics.
N. K. Mehra (2013)
Surface engineering of nanomaterials with phospholipid-polyethylene glycol-derived functional conjugates for molecular imaging and targeted therapy.
Dinglin Zhang (2019)
Potential Use of Biological Proteins for Liver Failure Therapy
K. Taguchi (2015)
Ceramic nanocarriers: versatile nanosystem for protein and peptide delivery
D. Singh (2013)
Decorating protein nanospheres with lactoferrin enhances oral COX-2 inhibitor/herbal therapy of hepatocellular carcinoma.
Mona A. Abdelmoneem (2018)
Cleavable PEGylation: a strategy for overcoming the “PEG dilemma” in efficient drug delivery
Y. Fang (2017)
Protection of SK-N-MC cells against β-amyloid peptide-induced degeneration using neuron growth factor-loaded liposomes with surface lactoferrin.
Y. Kuo (2014)
Tumor stromal disrupting agent enhances the anticancer efficacy of docetaxel loaded PEGylated liposomes in lung cancer.
Ketan Patel (2016)
Regression of prostate tumors after intravenous administration of lactoferrin-bearing polypropylenimine dendriplexes encoding TNF-α, TRAIL, and interleukin-12
Najla A. Altwaijry (2018)
In vitro and In vivo Studies on a Novel Bioadhesive Colloidal System: Cationic Liposomes of Ibuprofen
Xiumei Gai (2017)
Polylipid Nanoparticle, a Novel Lipid-Based Vector for Liver Gene Transfer
Yahan Fan (2013)See more