Online citations, reference lists, and bibliographies.
← Back to Search

Investigating The Cytotoxicity Of Iron Oxide Nanoparticles In In Vivo And In Vitro Studies.

S. Ghasempour, M. Shokrgozar, R. Ghasempour, M. Alipour
Published 2015 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
In recent years, iron oxide nanorods find a lot of applications including drug delivery, cell separation, hyperthermia and magnetic resonance imaging. In this study the cytotoxicity of iron oxide nanorods was evaluated based on mouse fibroblast cell behavior and wistar rat's liver and kidney function. At first for modification, nanorods were added to Dulbecco's modified Eagle's medium (DMEM) which contained a lot of sources of vitamins, amino acids, proteins in Fetal Bovine Serum (FBS). The MTT assay was employed for evaluating the toxic effects of 200 and 400 μg/mL modified and non-modified iron oxide nanorods on L929 mouse fibroblast cells in a 24h period. Changes in cell granularity and size as well as cell cycle were investigated using flow cytometry. Moreover liver and kidney function test and serum iron level measurement were performed 24h after the injection of modified iron oxide nanorods via the tail peripheral vein of wistar rats. Results indicated that greater concentration of modified iron oxide nanorods had no significant effect on cell viability while greater concentration of non-modified iron oxide nanorods significantly decreased cell viability. Modified iron oxide nanorods did not have significant effects on cell cycle. The results of liver and kidney function tests did not differ significantly while a significant increase in serum iron level was observed. After H&E staining of slices, there were no changes on morphology of rat's kidney and liver cells. This study suggests that short-time use of 200 and 400 μg/mL iron oxide nanorods are probably safe. Further studies are needed for investigation of toxic effects of different concentrations, coatings, and exposure time periods of iron oxide nanorods.
This paper references
Characterization and application of high magnetic property chitosan particles
Xiaoning An (2001)
How to commercialize nanotechnology
Michael N. Helmus (2006)
Systematic surface engineering of magnetic nanoworms for in vivo tumor targeting.
J. Park (2009)
Assessing the in vitro and in vivo toxicity of superparamagnetic iron oxide nanoparticles.
M. Mahmoudi (2012)
Superparamagnetic iron oxide nanoparticles: promises for diagnosis and treatment of multiple sclerosis.
M. Mahmoudi (2011)
Formulation of functionalized PLGA-PEG nanoparticles for in vivo targeted drug delivery.
J. Cheng (2007)
The promotion of human mesenchymal stem cell proliferation by superparamagnetic iron oxide nanoparticles.
D. Huang (2009)
Mineralization in ferritin: an efficient means of iron storage.
N. Chasteen (1999)
Graphene: promises, facts, opportunities, and challenges in nanomedicine.
H. Y. Mao (2013)
Peptide-labeled near-infrared quantum dots for imaging tumor vasculature in living subjects.
W. Cai (2006)
Implantable applications of chitin and chitosan.
E. Khor (2003)
Optimal design and characterization of superparamagnetic iron oxide nanoparticles coated with polyvinyl alcohol for targeted delivery and imaging.
M. Mahmoudi (2008)
A new approach for the in vitro identification of the cytotoxicity of superparamagnetic iron oxide nanoparticles.
M. Mahmoudi (2010)
A rapid lectin receptor binding assay: comparative evaluation of sea urchin embryo cell surface lectin receptors.
V. H. Latham (1995)
Long-Circulating Polymeric Nanovectors for Tumor-Selective Gene Delivery
S. Kommareddy (2005)
In vivo cancer targeting and imaging with semiconductor quantum dots
X. Gao (2004)
Magnetic resonance imaging of inducible E-selectin expression in human endothelial cell culture.
H. W. Kang (2002)
Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm.
T. Xia (2006)
Soft and Wet Materials: Polymer Gels
Y. Osada (1998)
Cytotoxicity and Cell Cycle Effects of Bare and Poly(vinyl alcohol)‐Coated Iron Oxide Nanoparticles in Mouse Fibroblasts
M. Mahmoudi (2009)
Surface characterisation of dextran-coated iron oxide nanoparticles prepared by laser pyrolysis and coprecipitation
M. C. Bautista (2005)
Surface-modified superparamagnetic nanoparticles for drug delivery: preparation, characterization, and cytotoxicity studies
A. Gupta (2004)
An in vitro study of bare and poly(ethylene glycol)-co-fumarate-coated superparamagnetic iron oxide nanoparticles: a new toxicity identification procedure.
M. Mahmoudi (2009)
Lactoferrin and ceruloplasmin derivatized superparamagnetic iron oxide nanoparticles for targeting cell surface receptors.
A. Gupta (2004)
Superparamagnetic iron oxide: pharmacokinetics and toxicity.
R. Weissleder (1989)
Preparation of a biocompatible magnetic film from an aqueous ferrofluid
C. Albornoz (2006)
Irreversible changes in protein conformation due to interaction with superparamagnetic iron oxide nanoparticles.
M. Mahmoudi (2011)
Effects of SiC nanoparticles orally administered in a rat model: biodistribution, toxicity and elemental composition changes in feces and organs.
Omar Lozano (2012)

This paper is referenced by
Iron Oxide Nanoparticles for Biomedical Applications: A Perspective on Synthesis, Drugs, Antimicrobial Activity, and Toxicity
L. S. Arias (2018)
Oxidative stress-mediated cytotoxicity of zirconia nanoparticles on PC12 and N2a cells
E. Asadpour (2016)
Towards a nanoparticle-based prophylactic for maternal autoantibody-related autism.
B. S. Amir Bolandparvaz (2019)
Glycine microparticles loaded with functionalized nanoparticles for pulmonary delivery.
Amlan Chakraborty (2019)
Irreversible disruption of the cytoskeleton as induced by non-cytotoxic exposure to titanium dioxide nanoparticles in lung epithelial cells.
Alejandro Déciga-Alcaraz (2020)
The analgesia efficiency of ultrasmall magnetic iron oxide nanoparticles in mice chronic inflammatory pain model.
P. Wu (2017)
Toxicity of nanostructures—a general approach
Cristina Ş. Iosub (2017)
Biodistribution and Toxicity of Epitope-functionalized Dextran Iron Oxide Nanoparticles in a Pregnant Murine Model.
Amir Bolandparvaz (2020)
Investigating Organ Toxicity Profile of Tenofovir and Tenofovir Nanoparticle on the Liver and Kidney: Experimental Animal Study
A. I. Peter (2018)
Synthesis and Characterization of Tea Polyphenol–Coated Magnetite Nanoparticles for Hyperthermia Application
Lavita Sarma (2019)
Fluorescent nanodiamonds as siRNA vectors : in vitro efficacy evaluation and high-content/high-resolution quantifications of their distribution in vivo. (Nanodiamants fluorescents pour la vectorisation de siRNA : évaluation in vitro et quantifications haut-débit/haute-résolution in vivo)
S. Claveau (2018)
Intracellular accumulation and immunological responses of lipid modified magnetic iron nanoparticles in mouse antigen processing cells.
Chenmeng Qiao (2017)
In-situ particles reorientation during magnetic hyperthermia application: Shape matters twice
K. Simeonidis (2016)
Semantic Scholar Logo Some data provided by SemanticScholar