← Back to Search
Toxicological Assessment Of Silica-coated Iron Oxide Nanoparticles In Human Astrocytes.
Natalia Fernández-Bertólez, C. Costa, Fátima Brandão, Gözde Kiliç, J. A. Duarte, J. Teixeira, E. Pásaro, Vanessa Valdiglesias, B. Laffon
Published 2018 · Political Science, Medicine
Download PDFAnalyze on Scholarcy
Iron oxide nanoparticles (ION) have great potential for an increasing number of medical and biological applications, particularly those focused on nervous system. Although ION seem to be biocompatible and present low toxicity, it is imperative to unveil the potential risk for the nervous system associated to their exposure, especially because current data on ION effects on human nervous cells are scarce. Thus, in the present study potential toxicity associated with silica-coated ION (S-ION) exposure was evaluated on human A172 glioblastoma cells. To this aim, a complete toxicological screening testing several exposure times (3 and 24 h), nanoparticle concentrations (5-100 μg/ml), and culture media (complete and serum-free) was performed to firstly assess S-ION effects at different levels, including cytotoxicity - lactate dehydrogenase assay, analysis of cell cycle and cell death production - and genotoxicity - H2AX phosphorylation assessment, comet assay, micronucleus test and DNA repair competence assay. Results obtained showed that S-ION exhibit certain cytotoxicity, especially in serum-free medium, related to cell cycle disruption and cell death induction. However, scarce genotoxic effects and no alteration of the DNA repair process were observed. Results obtained in this work contribute to increase the knowledge on the impact of ION on the human nervous system cells.
This paper references
Uptake and Metabolism of Iron Oxide Nanoparticles in Brain Cells
Charlotte Petters (2014)
Cytotoxicity and genotoxicity
O. Witschger (2012)
S. Sridhar (2015)
Bone tissue engineering with novel rhBMP2-PLLA composite scaffolds.
Po-Chun Chang (2007)
Diversity of DNA damage response of astrocytes and glioblastoma cell lines with various p53 status to treatment with etoposide and temozolomide
Y. Sato (2009)
Effects of Iron-Oxide Nanoparticle Surface Chemistry on Uptake Kinetics and Cytotoxicity in CHO-K1 Cells
Camille C. Hanot (2015)
In vivo nanoneurotoxicity screening using oxidative stress and neuroinflammation paradigms.
Youngsoon Kim (2013)
Cytotoxicity and genotoxicity of nanosized and microsized titanium dioxide and iron oxide particles in Syrian hamster embryo cells.
Y. Guichard (2012)
VEGF-targeted magnetic nanop
V. P. Chekhonin (2015)
Magnetite induces o
G. T. Ramesh (2012)
The comet assay in nanotoxicology research
H. Karlsson (2010)
Iron Oxide Nanoparticles Induce Dopaminergic Damage: In vitro Pathways and In Vivo Imaging Reveals Mechanism of Neuronal Damage
S. Imam (2015)
ERK pathway is activated in bare-FeNPs-induced autophagy
E. Park (2013)
Endocytotic uptake of iron oxide nanoparticles by cultured brain microglial cells.
E. M. Luther (2013)
In vivo na - noneurotoxicity screening using oxidative stress and neuroin fl ammation paradigms
Iron Oxide Nanoparticles Indu
Z. K. Binienda (2015)
Effects of iron oxide nanoparticles: Cytotoxicity, genotoxicity, developmental toxicity, and neurotoxicity
Vanessa Valdiglesias (2015)
Development of in vitro systems for nanotoxicology: methodological considerations
V. Stone (2009)
Synthesis and characterization of magnetic nanoparticles coated with silica through a sol-gel approach
Â. L. Andrade (2009)
The role of iron redox state in the genotoxicity of ultrafine superparamagnetic iron oxide nanoparticles.
N. Singh (2012)
Effects of Iron-Oxide
A. avid (2015)
A perspective on the assessment of nanoparticle toxicity in vitro: Current status and furutre prospects of assessing iron oxide nanoparticle toxicity in vitro
S. Soenen (2010)
Current investigations into magnetic nanoparticles for biomedical applications.
X. Li (2016)
Synthesis and surface engineering of iron oxide nano
A. K. mes006. Gupta (2005)
Magnetite Nanoparticles Induce Genotoxici
D. Nakae (2014)
The comet assay in nanotoxico
H. L. Karlsson (2010)
The Comet Assay
G. Speit (2005)
Cytotoxic effect of magneti
M. Mahdavi (2014)
R. A. Revia (2016)
J. T. Rosenberg (2012)
Astrocytes: New Targets for the Treatment of Neurodegenerative Diseases.
Charles Finsterwald (2015)
Assessing iron oxide nanoparticle toxicity in vitro: current status and future prospects.
Stefaan J.H. Soenen (2010)
Are iron oxide nanoparticles safe
Magnetic properties of SiO2-coated iron oxide nanoparticles studied by polarized small angle neutron scattering
S. Lee (2015)
Astrocytes in neurodegenerative disease.
H. Phatnani (2015)
Nanoparticle Architectures Templated by SiO2/Fe2O3 Nanocomposites
D. Yi (2006)
Assessing cytotoxicity of (iron oxide-based) nanoparticles: an overview of different methods exemplified with cationic magnetoliposomes.
S. Soenen (2009)
VEGF-targeted magnetic nanoparticles for MRI visualization of brain tumor.
Maxim A. Abakumov (2015)
Oligodendrocytes and Alzhe
Z. Cai (2016)
The impact of species
Induction of oxidative stress, DNA damage, and apoptosis in a malignant human skin melanoma cell line after exposure to zinc oxide nanoparticles
S. Alarifi (2013)
Fabrication and neuron cytocompatibility of iron oxide nanoparticles coated with silk-fibroin peptides.
M. Deng (2014)
V. ti (2014)
DNA Double-stranded Breaks Induce Histone H2AX Phosphorylation on Serine 139*
E. Rogakou (1998)
A simple technique for quantitation of low levels of DNA damage in individual cells.
N. Singh (1988)
Intracellular SPIO labeling of microglia: high field considerations and limitations for MR microscopy.
J. Rosenberg (2012)
Synthesis and surfac
A. K. Gupta (2005)
Effects of iron oxide nanoparticl
N. ertolez (2014)
S.J.H. Soenen (2010)
S. C. Grant (2012)
Evaluation on cytotoxicity and genotoxicity of the L-glutamic acid coated iron oxide nanoparticles.
T. Zhang (2012)
Neurodegenerative disorders: the Glia way forward
R. Barker (2014)
Comparative study of human neuronal and glial cell sensitivity for in vitro neuro - genotoxicity testing
B. La ff on
Chemosensitivity of glioma cells in vitro: a meta analysis
Johannes E. A. Wolff (1999)
Isolation and characterization of cancer stem like cells in human glioblastoma cell lines.
L. Qiang (2009)
S. S. Lee (2006)
Nanoparticles and Neurotoxicity
Tin-Tin Win-Shwe (2011)
Oligodendrocytes and Alzheimer's disease
Zhiyou Cai (2016)
The comet assay, DNA damage, DNA repair and cytotoxicity: hedgehogs are not always dead.
Y. Lorenzo (2013)
The journey of a drug-carrier in the body: an anatomo-physiological perspective.
N. Bertrand (2012)
Potential toxicity of superparamagnetic iron oxide nanoparticles (SPION)
N. Singh (2010)
Toxicity of Metal Oxide Nanoparticles in Mammalian Cells
H. Jeng (2006)
The micronucleus assay determination of chromosomal level DNA damage.
M. Fenech (2008)
Neuronal cytotoxicity and genotoxicity induced by zinc oxide nanoparticles.
Vanessa Valdiglesias (2013)
Design of iron oxide-based nanoparticles for MRI and magnetic hyperthermia.
Cristina Blanco-Andujar (2016)
Synthesis and characterization of magnetic nanoparticles coat
Astrocytes in neu
H. Phatnani (2015)
Rhamnose‐coated superparamagnetic iron‐oxide nanoparticles: an evaluation of their in vitro cytotoxicity, genotoxicity and carcinogenicity
Alessandro Paolini (2016)
Understanding biophysicochemical interactions at the nano-bio interface.
A. Nel (2009)
Magnetite induces oxidative stress and apoptosis in lung epithelial cells
V. Ramesh (2011)
Effects of iron-oxide
Y. Choi (2015)
Okadaic acid induces morpho
B. Laffon (2011)
Hybrid protein-inorganic nanoparticles
A. O. Elzoghby (2016)
Genotoxic and carcinogenic potential of engineered nanoparticles: an update
A. Kumar (2013)
Cytotoxicity of Uncoated and Polyvinyl Alcohol Coated Superparamagnetic Iron Oxide Nanoparticles
M. Mahmoudi (2009)
Accumulation of iron oxide nanoparticles by cultured brain astrocytes.
M. Geppert (2009)
Modification of the surface of superparamagnetic iron oxide nanoparticles to enable their safe application in humans
C. Strehl (2016)
Coating-dependent induction of cytotoxicity and genotoxicity of iron oxide nanoparticles
Z. Magdolénová (2015)
Serum heat inactivation affects protein corona composition and nanoparticle uptake.
A. Leśniak (2010)
Handling of Iron Oxide and Silver Nanoparticles by Astrocytes
M. Hohnholt (2012)
Titanium dioxide nanoparticles induce oxidative stress and DNA-adduct formation but not DNA-breakage in human lung cells
K. Bhattacharya (2008)
Silica nanoparticle size influences the structure and enzymatic activity of adsorbed lysozyme.
A. Vertegel (2004)
Fabrication and neuron
Cell "vision": complementary factor of protein corona in nanotoxicology.
M. Mahmoudi (2012)
Cytotoxic effect of magnetic iron oxide nanoparticles synthesized via seaweed aqueous extract
F. Namvar (2014)
Ferritin up-regulation and transient ROS production in cultured brain astrocytes after loading with iron oxide nanoparticles.
Mark J. Geppert (2012)
Biomolecular coronas provide the biological identity of nanosized materials.
M. Monopoli (2012)
Uptake of dimercaptosuccinate-coated magn
I. S. Grunwald (2011)
ff ects of Fe 3 O 4 magnetic nanoparticles on A 549 cells
Can Standard Genotoxicity Tests be Applied to Nanoparticles?
Z. Magdolénová (2012)
Astrocytes: new targets
C. 012. Finsterwald (2015)
Neurological disorders and therapeutics
B. Sriramoju (2012)
γ H 2 AX assay as DNA damage biomarker for human population studies : de fi ning experimental conditions
A. K. Gupta (2005)
dextran - coated superparamagnetic iron oxide nanoparticles ( SPION ) in HepG 2 cells using the comet assay and cytokinesis - block micronucleus assay
N. Singh (2010)
Nanomaterials and neurodegeneration
Lucia Migliore (2015)
Comparative cytotoxicity and genotoxicity of cobalt (II, III) oxide, iron (III) oxide, silicon dioxide, and aluminum oxide nanoparticles on human lymphocytes in vitro
S. Rajiv (2016)
Cell Cycle: Principles of Control
M. E. Crosby (2007)
Ferucarbotran (Resovist): a new clinically approved RES-specific contrast agent for contrast-enhanced MRI of the liver: properties, clinical development, and applications
P. Reimer (2002)
Induction of oxidative stress, DNA damage, and apop
M. med (2013)
Effects of Fe3O4 Magnetic Nanoparticles on A549 Cells
Masatoshi Watanabe (2013)
Magnetite nanoparticles for cancer diagnosis, treatment, and treatment monitoring: recent advances.
R. Revia (2016)
Synthesis, Surface Modification and Characterisation of Biocompatible Magnetic Iron Oxide Nanoparticles for Biomedical Applications
Mahnaz Mahdavi (2013)
M. Sánchez-Flores (2015)
Okadaic acid induces morphological changes, apoptosis and cell cycle alterations in different human cell types.
Vanessa Valdiglesias (2011)
Genotoxic and carcinog
A. Dhawan (2013)
Neurological disorders and therapeutics targeted to surmount the blood–brain barrier
J. Kanwar (2012)
Comparative study of human neuronal and glial cell sensitivity for in vitro neurogenotoxicity testing.
B. Laffon (2017)
Magnetite Nanoparticles Induce Genotoxicity in the Lungs of Mice via Inflammatory Response
Yukari Totsuka (2014)
Targeted Drug Delivery to the Brain Using Focused Ultrasound
M. Kinoshita (2006)
Protein-nanoparticle interactions: opportunities and challenges.
M. Mahmoudi (2011)
Investigation of the genetic toxicity by dextran-coated superparamagnetic iron oxide nanoparticles (SPION) in HepG2 cells using the comet assay and cytokinesis-block micronucleus assay
Do Yeon Seo (2017)
M. Duggal (2005)
Investigation on mechanism
J. Wu (2011)
Y. Kim (2014)
Effect of surface coating on the biocompatibility and in vivo MRI detection of iron oxide nanoparticles after intrapulmonary administration
A. Al Faraj (2015)
Are iron oxide nanoparticles safe? Current knowledge and future perspectives.
Vanessa Valdiglesias (2016)
Iron oxide nanoparticles induce dopaminergic damage: in vitro
Z. K. Binienda (2015)
Subtle cytotoxicity and genotoxicity differences in superparamagnetic iron oxide nanoparticles coated with various functional groups
Seong Cheol Hong (2011)
Toxicity Assessment of Silica Coated Iron Oxide Nanoparticles and Biocompatibility Improvement by Surface Engineering
M. A. Malvindi (2014)
Investigation on mechanism of growth arrest induced by iron oxide nanoparticles in PC12 cells.
J. Wu (2011)
Genotoxicity assessment of magnetic iron oxide nanoparticles with different particle sizes and surface coatings.
Y. Liu (2014)
Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications.
A. Gupta (2005)
ff ects of iron - oxide nanoparticle surface chemistry on uptake kinetics and cytotoxicity in CHOK 1 cells
New England Journal of Medicine: Clinical Problem-Solving
L. K. Tom (2007)
Superparamagnetic iron oxide nanoparticles for delivery of therapeutic agents: opportunities and challenges
S. Laurent (2014)
Mechanisms of genotoxicity. A review of in vitro and in vivo studies with engineered nanoparticles
Z. Magdolénová (2014)
USPIO‐Enhanced MRI Neuroimaging: A Review
Maria Gkagkanasiou (2016)
Neuronal cytotoxicity and genotoxicity
E. Pásaro (2013)
In vitro toxicity evaluatio
V. Valdiglesias (2015)
Correction: In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells
Gözde Kiliç (2016)
Modification of the surface
F. Buttgereit (2016)
Uptake of dimercaptosuccinate-coated magnetic iron oxide nanoparticles by cultured brain astrocytes.
Mark J. Geppert (2011)
γH2AX assay as DNA damage biomarker for human population studies: defining experimental conditions.
María Sánchez-Flores (2015)
Neurotoxic potential of iron oxide nanoparticles in the rat brain striatum and hippocampus.
J. Wu (2013)
Alternating Magnetic Field-Induced Hyperthermia Increases Iron Oxide Nanoparticle Cell Association/Uptake and Flux in Blood–Brain Barrier Models
M. Dan (2014)
Targeted drug delivery to the brain using magnetic nanoparticles.
L. B. Thomsen (2015)
In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests
C. Costa (2016)
Silica-coated super paramagnetic iron oxide nanoparticles (SPION) as biocompatible contrast agent in biomedical photoacoustics
Rudolf Alwi (2012)
Hybrid protein-inorganic nanoparticles: From tumor-targeted drug delivery to cancer imaging.
Ahmed O Elzoghby (2016)
A simple technique
N. P. Singh (1988)
Toxicity of metal ox
H. A. Jeng (2006)
This paper is referenced by
Fe3O4 Nanoparticles for Complex Targeted Delivery and Boron Neutron Capture Therapy
K. Dukenbayev (2019)
Assessment of oxidative damage induced by iron oxide nanoparticles on different nervous system cells.
Natalia Fernández-Bertólez (2019)
Evaluation of cytotoxicity and genotoxicity induced by oleic acid‐coated iron oxide nanoparticles in human astrocytes
Natalia Fernández-Bertólez (2019)
Quercetin conjugated with superparamagnetic iron oxide nanoparticles improves learning and memory better than free quercetin via interacting with proteins involved in LTP
Elnaz Amanzadeh (2019)
Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro
J. Limón-Pacheco (2020)
Nanoformulations for glioblastoma multiforme: a new hope for treatment.
R. Ortiz (2019)
Toxicity Assessment of Nanomaterials
M. Tasso (2020)
The Investigation into the Toxic Potential of Iron Oxide Nanoparticles Utilizing Rat Pheochromocytoma and Human Neural Stem Cells
Weili Ma (2019)
Optimization of the harvesting and freezing conditions of human cell lines for DNA damage analysis by the alkaline comet assay.
M. J. Bessa (2019)
Brain-Targeted Drug Delivery with Surface-Modified Nanoparticles
Sunita Lahkar (2019)
Comet assay in neural cells as a tool to monitor DNA damage induced by chemical or physical factors relevant to environmental and occupational exposure.
M. Kruszewski (2019)
Effect of size and silica coating on structural, magnetic as well as cytotoxicity properties of copper ferrite nanoparticles.
L. Khanna (2019)
More data on in vitro assessment of comparative and combined toxicity of metal oxide nanoparticles.
T. Bushueva (2019)
Immobilization of boron-rich compound on Fe3O4 nanoparticles: Stability and cytotoxicity
D. Tishkevich (2019)