Online citations, reference lists, and bibliographies.

Two Lung Development-related MicroRNAs, MiR-134 And MiR-187, Are Differentially Expressed In Lung Tumors.

Fatemeh Mirzadeh Azad, Parisa Naeli, Mahshid Malakootian, Alireza Baradaran, Mahmoud Tavallaei, Mostafa Ghanei, Sj Mowla
Published 2016 · Medicine, Biology
Cite This
Download PDF
Analyze on Scholarcy
INTRODUCTION MicroRNAs (miRNAs) are involved in various cellular events needed for embryonic development and tumorigenesis. As some of the development-specific gene expression patterns could be observed in cancers, we speculated that the expression pattern of lung development-specific miRNAs miR-134 and miR-187 might be altered in lung tumor samples. Lung cancer is the first cause of cancer related deaths worldwide, mostly due to its late diagnosis. Therefore, finding a reliable diagnostic tumor marker, based on molecular profile of tumorigenesis, would be critical in lowering lung cancer mortality. METHODS We employed a real-time RT-PCR approach to evaluate the expression alteration of two lung development-related miRNAs in lung tumor tissues. The suitability of miRs expression alterations as lung tumor biomarkers was tested by receiver operating characteristic (ROC) curve analysis. The effect of miR-187 overexpression on a lung carcinoma cell cycle was assessed using flow cytometry analysis. RESULTS Our data revealed a significant upregulation (7.8 times, p<0.02) of miR-134 in lung tumors. However, its expression level failed to discriminate different tumor types and grades of malignancies from each other. Moreover, the ROC curves analysis did not give it a good score as a reliable biomarker (AUC=0.522, P=0.729). In contrast, miR-187 showed a significant down-regulation (P=0.008) in lung tumors. Similarly, its expression level failed to differentiate different tumor types or grades of malignancies. Nevertheless, ROC curve analysis gave it an AUC score of 0.669 (P=0.012), which suggests its suitability as a potential biomarker for lung cancer. Furthermore, ectopic expression of miR-187 in A549 cells caused a cell cycle arrest in G1 phase (P=0.013). CONCLUSION Altogether, our data demonstrated an altered expression of two development-related miRNAs namely miR-134 and miR-187 in lung tumors for the first time. Moreover we have shown that miR-134 and miR-187 expression alternation were in accordance with their approved regulatory roles, therefore these miRNAs could serve as new biomarkers with potential usefulness in lung cancer diagnosis and treatments. In addition, miR-187 expression in tumor cells could perturb cell cycle which supported its possible role as tumor suppressor.
This paper references
MicroRNA-127 modulates fetal lung development.
Manoj Bhaskaran (2009)
MiR-134 functions as a regulator of cell proliferation, apoptosis, and migration involving lung septation
Xiaoying Zhang (2012)
Intersections between pulmonary development and disease.
J. Whitsett (2011)
Cancer biomarkers: a systems approach
L. Hartwell (2006)
Early Lung Cancer Diagnosis by Biosensors
Yuqian Zhang (2013)
B7-H3 silencing by RNAi inhibits tumor progression and enhances chemosensitivity in U937 cells
Wei Zhang (2015)
MicroRNA-187, down-regulated in clear cell renal cell carcinoma and associated with lower survival, inhibits cell growth and migration though targeting B7-H3.
Jun Zhao (2013)
Environmental risk factors for lung cancer in Iran: a case-control study.
M. Hosseini (2009)
MiR-134-mediated β1 integrin expression and function in mesenchymal stem cells.
David M. Poitz (2013)
miR-134 induces oncogenicity and metastasis in head and neck carcinoma through targeting WWOX gene.
Chung-Ji Liu (2014)
MicroRNAs Genomics, Biogenesis, Mechanism, and Function
D. Bartel (2004)
Causes and consequences of microRNA dysregulation in cancer
C. Croce (2009)
miRiad Roles for the miR-17-92 Cluster in Development and Disease
J. Mendell (2008)
Complex networks orchestrate epithelial–mesenchymal transitions
J. Thiery (2006)
MicroRNA function in cancer: oncogene or a tumor suppressor?
Sylvia K. Shenouda (2009)
Regulation of PTEN binding to MAGI-2 by two putative phosphorylation sites at threonine 382 and 383.
T. Tolkacheva (2001)
Induced expression of B7-H3 on the lung cancer cells and macrophages suppresses T-cell mediating anti-tumor immune response.
Cheng Chen (2013)
Most mammalian mRNAs are conserved targets of microRNAs.
Robin C Friedman (2009)
Disabled-2 (Dab2) Is Required for Transforming Growth Factor β-induced Epithelial to Mesenchymal Transition (EMT)*
Céline Prunier (2005)
Dicer function is essential for lung epithelium morphogenesis
K. S. Harris (2006)
WWOX gene restoration prevents lung cancer growth in vitro and in vivo
M. Fabbri (2005)
microRNA Biogenesis and Function : An overview.
R. Ketting (2011)
Functional association between Wwox tumor suppressor protein and p73, a p53 homolog.
R. Aqeilan (2004)
Binding of PTEN to Specific PDZ Domains Contributes to PTEN Protein Stability and Phosphorylation by Microtubule-associated Serine/Threonine Kinases*
Miguel Valiente (2005)
Stage-specific modulation of cortical neuronal development by Mmu-miR-134.
Philip Michael Gaughwin (2011)
WW domain containing oxidoreductase gene expression is altered in non-small cell lung cancer.
Sai Yendamuri (2003)
MicroRNA in lung cancer
P-Y Lin (2010)
MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation
Yvonne Tay (2009)
The contrasting role of B7-H3
Kimberly A. Hofmeyer (2008)
Identifying microRNAs regulating B7-H3 in breast cancer: the clinical impact of microRNA-29c
M. K. Nygren (2014)
Correction: Tissue and Process Specific microRNA–mRNA Co-Expression in Mammalian Development and Malignancy
Hongye Liu (2009)
Non-small cell lung cancer.
D. Ettinger (2012)
MicroRNA-31 functions as an oncogenic microRNA in mouse and human lung cancer cells by repressing specific tumor suppressors.
X. Liu (2010)
A novel pathway regulates memory and plasticity via SIRT1 and miR-134
Jun Gao (2010)
Early detection of lung cancer: role of biomarkers
Christian G Brambilla (2003)
Fragile genes as biomarkers: epigenetic control of WWOX and FHIT in lung, breast and bladder cancer
Dimitrios Iliopoulos (2005)
Tissue and Process Specific microRNA–mRNA Co-Expression in Mammalian Development and Malignancy
Hongye Liu (2009)
Lung cancer.
R. Herbst (2008)
Regulation of ovarian cancer progression by microRNA-187 through targeting Disabled homolog-2
A Chao (2012)
MiR-134/487b/655 Cluster Regulates TGF-β–Induced Epithelial–Mesenchymal Transition and Drug Resistance to Gefitinib by Targeting MAGI2 in Lung Adenocarcinoma Cells
Kazuhiro Kitamura (2013)
Molecular profiling of mouse lung tumors: association with tumor progression, lung development, and human lung adenocarcinomas
Allison E Bonner (2004)
bantam Encodes a Developmentally Regulated microRNA that Controls Cell Proliferation and Regulates the Proapoptotic Gene hid in Drosophila
J. Brennecke (2003)
Maternally imprinted microRNAs are differentially expressed during mouse and human lung development.
A. Williams (2007)

This paper is referenced by
miR-134 targets PDCD7 to reduce E-cadherin expression and enhance oral cancer progression.
Shih-Yuan Peng (2018)
Comparison of microRNA transcriptomes reveals differential regulation of microRNAs in different-aged boars.
Xue-Yu Xu (2018)
The role of microRNAs in Lung Cancer: Implications for diagnosis and therapy.
Parisa Naeli (2019)
miR-3175 and miR-134 affect proliferation, invasion and apoptosis of glioma cells through PI3K/AKT signaling pathway.
Aiqin Qi (2019)
MicroRNA-187-3p mitigates non-small cell lung cancer (NSCLC) development through down-regulation of BCL6.
Chengcao Sun (2016)
MicroRNA-187 Reduces Acute Ischemic Renal Podocyte Injury via Targeting Acetylcholinesterase.
J. Yue (2019)
Association of KRAS rs 712 polymorphism and cancer risk in Chinese population : a meta-analysis
Yanjun Lu (2016)
Estudio del gen YKT6 y miRNAs asociados en la liberación de exosomas en pacientes quirúrgicos de cáncer de pulmón
Marc Ruiz Martinez (2016)
A universal electrochemical biosensor for the highly sensitive determination of microRNAs based on isothermal target recycling amplification and a DNA signal transducer triggered reaction
Lili Zhou (2017)
MicroRNA‑187 is an independent prognostic factor in lung cancer and promotes lung cancer cell invasion via targeting of PTRF.
Jie Peng (2016)
miR-134: A Human Cancer Suppressor?
Jingyu Pan (2017)
MicroRNA-187 inhibits tumor growth and metastasis via targeting of IGF-1R in hepatocellular carcinoma.
Xinqiang Han (2017)
MicroRNA-187-5p suppresses cancer cell progression in non-small cell lung cancer (NSCLC) through down-regulation of CYP1B1.
Ming Ming Mao (2016)
YKT6 expression, exosome release, and survival in non-small cell lung cancer
Marc Ruíz-Martínez (2016)
High miRNA-221 expression is associated with poor overall survival in patients with non-small cell lung cancer
Y. Liu (2017)
Semantic Scholar Logo Some data provided by SemanticScholar