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Chitosan Extracted From Aspergillus Flavus Shows Synergistic Effect, Eases Quorum Sensing Mediated Virulence Factors And Biofilm Against Nosocomial Pathogen Pseudomonas Aeruginosa.

Sahira Nsayef Muslim, I. M. A. Kadmy, Alaa Naseer Mohammed Ali, Batool Kadham Salman, M. Ahmad, S. S. Khazaal, Nadheema Hammood Hussein, S. N. Muslim
Published 2018 · Biology, Medicine

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The biological methods for extraction of chitosan were used as alternative procedures for chemical methods In biological methods, the chitosan was extracted from A. flavus by using of Lactobacillus paracasei for demineralization and Bacillus subtilis for deproteinization. The yield of extracted chitosan reached to 53.8%, pH was 7.8 and complete solubility in 1% acitic acid. Purified chitosan had the ability to reduce the biofilm forming capacity of P. aeruginosa clearly visible in light microscopic staining and scanning electron microscopy. The QS dependent phenotype and QS regulated gene expression was significantly reduced in the influence of chitosan. A significant decrease in biofilm formation was seen in the presence of chitosan. The chitosan treatment showed a decrease in the expression of lasR and rhlR genes. Same time production of pyocyanin and proteases was also inhibited in dose dependent manner. Chitosan led to increasing antimicrobial activity of antibiotics and had synergism effect, thus chitosan may be a useful adjuvant agent for the treatment of many bacterial infections in combination with antibiotics.
This paper references
The quorum sensing molecule N-acyl homoserine lactone produced by Acinetobacter baumannii displays antibacterial and anticancer properties
James John (2016)
Improving of Antibacterial Activity for Antibiotics by Purified and Characterized Lectin from Acinetobacter baumannii
Sahira Nsayef Muslim (2015)
Antibacterial effects of N-sulfonated and N-sulfobenzoyl chitosan and application to oyster preservation.
C. Chen (1998)
Synthesis and antibacterial activities of quaternary ammonium salt of chitosan.
Z. Jia (2001)
Antibiotic-Resistant Bacteria
Patrick Guilfoile (2006)
Synthesis and characterization of chitosan from marine sources in Black Sea
D. Zvezdova (2010)
Fusarium species: an illustrated manual for identification.
P. Nelson (1983)
Molecular Weight Dependency of Antimicrobial Activity by Chitosan Oligomers
S. Sekiguchi (1994)
Biosafety of handling with some pathogenic bacteria
Alaa Naseer Mohammed Ali (2017)
Chitosan as an enabling excipient for drug delivery systems. I. Molecular modifications.
S. Sabnis (2000)
Antimicrobial effect of chitooligosaccharides produced by bioreactor
Y. Jeon (2001)
Antimicrobial Resistance and Implications for the Twenty-First Century
I. Fong (2008)
A Biopolymer Chitosan and Its Derivatives as Promising Antimicrobial Agents against Plant Pathogens and Their Applications in Crop Protection
M. Badawy (2011)
The hierarchy quorum sensing network in Pseudomonas aeruginosa
J. Lee (2014)
Production of Fungal Chitosan by Enzymatic Method and Applications in Plant Tissue Culture and Tissue Engineering: 11 Years of Our Progress, Present Situation and Future Prospects
N. Nwe (2010)
Antimicrobial activity of native chitosan, degraded chitosan, and O-carboxymethylated chitosan.
K. W. Kim (2003)
Chitosan as antimicrobial agent: applications and mode of action.
E. Rabea (2003)
Fungal chitosan production and its characterization
P. Pochanavanich (2002)
Study on antimicrobial activity of chitosan with different molecular weights
lian-ying Zheng (2003)
Identification of polyphenols in tobacco leaf and their antioxidant and antimicrobial activities
H. Wang (2008)
Encyclopedia of Food Microbiology
C. Batt (2000)
Unlocking the Secrets Behind Secondary Metabolism: A Review of Aspergillus flavus from Pathogenicity to Functional Genomics
K. Scheidegger (2003)
Demineralization of red crab shell waste by lactic acid fermentation
W. Jung (2004)

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