Online citations, reference lists, and bibliographies.
← Back to Search

Electromagnetic Manipulation Enabled Calcium Alginate Janus Microsphere For Targeted Delivery Of Mesenchymal Stem Cells.

R. Thomas, A. R. Unnithan, Myeong Ju Moon, Suchithra Poilil Surendran, Tumurbaatar Batgerel, C. Park, C. Kim, Y. Jeong
Published 2018 · Medicine, Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
We prepared Janus microspheres based on sodium alginate for the encapsulation of mesenchymal stem cells (MSC) in one compartment and iron oxide nanoparticles (IONP) or a drug in the second compartment. 4% percent sodium alginate solution was allowed to pass through a septum-theta capillary device and react with 2.5% calcium chloride to allow crosslinking to occur in the solution, forming calcium alginate Janus microspheres. Physico-chemical characterization of microspheres was done by FTIR, TGA, and XRD after loading of stem cells and IONP/drug. The mechanical integrity of microspheres was tested at different time points, which showed that 4% alginate microspheres were mechanically stable for a long period of time. Live/dead staining of MSCs alone and the MTS assay of MSCs and DMSO co-loaded were performed, which showed less toxicity to MSC in the Janus configuration. IONP/MSC-loaded Janus microspheres were tested by magnetic manipulation for targeted MSC delivery for cartilage repair using an electromagnetic manipulation (EMM) device. Janus microspheres can be used for targeted stem cell/drug delivery using EMM for cartilage repair in the near future.
This paper references
10.1177/0363546513483270
Articular Cartilage Repair With Magnetic Mesenchymal Stem Cells
G. Kamei (2013)
10.1016/J.PROGPOLYMSCI.2011.06.003
Alginate: properties and biomedical applications.
K. Lee (2012)
10.15272/AJBPS.V5I47.713
Microsphere: A Brief Review
N. Kadam (2015)
10.1159/000335697
Liver Regenerative Medicine: Advances and Challenges
D. Chistiakov (2012)
10.1016/j.xphs.2015.11.019
Intra-articular Administration of Chitosan Thermosensitive In Situ Hydrogels Combined With Diclofenac Sodium-Loaded Alginate Microspheres.
X. Qi (2016)
10.1039/C6RA20884C
Revival, enhancement and tuning of fluorescence from Coumarin 6: combination of host–guest chemistry, viscosity and collisional quenching
R. Banerjee (2016)
10.1002/adhm.201601378
A Magnetically Actuated Microscaffold Containing Mesenchymal Stem Cells for Articular Cartilage Repair
G. Go (2017)
10.1016/j.actbio.2012.03.045
In vitro feasibility study of the use of a magnetic electrospun chitosan nanofiber composite for hyperthermia treatment of tumor cells.
Ta-chun Lin (2012)
10.1021/acs.langmuir.7b01123
Janus Particle Synthesis, Assembly, and Application.
J. Zhang (2017)
10.3748/wjg.v21.i3.742
Use of mesenchymal stem cells to treat liver fibrosis: current situation and future prospects.
S. Berardis (2015)
10.1186/s12951-016-0213-x
Labeling mesenchymal cells with DMSA-coated gold and iron oxide nanoparticles: assessment of biocompatibility and potential applications
Luisa H. A. Silva (2016)
10.1016/j.actbio.2009.07.042
Microsphere-based scaffolds for cartilage tissue engineering: using subcritical CO(2) as a sintering agent.
M. Singh (2010)
10.1021/la903348s
Multifunctional superparamagnetic Janus particles.
Kai P Yuet (2010)
10.1016/j.msec.2016.02.071
Injectable alginate/hydroxyapatite gel scaffold combined with gelatin microspheres for drug delivery and bone tissue engineering.
Jingxuan Yan (2016)
10.1002/adma.201102560
Controlled synthesis of 3D multi-compartmental particles with centrifuge-based microdroplet formation from a multi-barrelled capillary.
K. Maeda (2012)
10.1039/c5nr05844a
A smart magnetic nanoplatform for synergistic anticancer therapy: manoeuvring mussel-inspired functional magnetic nanoparticles for pH responsive anticancer drug delivery and hyperthermia.
A. Sasikala (2015)
10.3390/ma7010275
Alginate-Poly(ethylene glycol) Hybrid Microspheres for Primary Cell Microencapsulation
R. Mahou (2014)
10.3904/kjim.2015.30.5.580
Mesenchymal stem cell therapy for liver fibrosis
Y. W. Eom (2015)
10.1002/ADFM.200801181
Generation of Monodisperse Inorganic–Organic Janus Microspheres in a Microfluidic Device
N. Prasad (2009)
10.1038/nrd.2016.232
Advances in islet encapsulation technologies
T. Desai (2017)
10.1039/C5RA03862F
Production of ultra-high concentration calcium alginate beads with prolonged dissolution profile
Wan Ping Voo (2015)
10.1002/bit.24752
Co‐culture with mesenchymal stem cells enhances metabolic functions of liver cells in bioartificial liver system
Y. Yang (2013)
10.1021/am502319m
Janus-compartmental alginate microbeads having polydiacetylene liposomes and magnetic nanoparticles for visual lead(II) detection.
Do Hyun Kang (2014)
10.1016/j.jhep.2014.10.030
Microencapsulated human mesenchymal stem cells decrease liver fibrosis in mice.
R. Meier (2015)
10.1016/j.arthro.2007.08.017
A novel cell delivery system using magnetically labeled mesenchymal stem cells and an external magnetic device for clinical cartilage repair.
Takaaki Kobayashi (2008)
10.1016/J.BIOMATERIALS.2007.07.041
Self-assembled collagen-human mesenchymal stem cell microspheres for regenerative medicine.
B. Chan (2007)
10.2528/PIERM14042907
Application of Pulsed Magnetic Ponderomotive Force for Intra-Cellular Gene Delivery
Peter Babinec (2014)
10.1155/2014/429710
Transplantation of Encapsulated Pancreatic Islets as a Treatment for Patients with Type 1 Diabetes Mellitus
M. Qi (2014)
10.4291/wjgp.v3.i1.19
Encapsulated islets transplantation: Past, present and future.
N. Sakata (2012)
10.1371/journal.pone.0138184
In Situ Transplantation of Alginate Bioencapsulated Adipose Tissues Derived Stem Cells (ADSCs) via Hepatic Injection in a Mouse Model
Mong-Jen Chen (2015)
10.1002/ADV.21276
Pharmaceutical applications of biocompatible polymer blends containing sodium alginate
A. Dalmoro (2012)
10.1517/14712598.3.2.201
Alginate microcapsules for pancreatic islet cell graft immunoprotection: struggle and progress towards the final cure for type 1 diabetes mellitus
R. Calafiore (2003)
10.1517/14712598.4.1.35
Microspheres for controlled release drug delivery
Neelesh K Varde (2004)
10.5500/wjt.v6.i1.69
Survival of encapsulated islets: More than a membrane story.
U. Barkai (2016)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar