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Characterisation Of Chitosan Molecular Weight Distribution By Multi-detection Asymmetric Flow-field Flow Fractionation (AF4) And SEC.

Y. González-Espinosa, B. Sabagh, E. Moldenhauer, P. Clarke, F. Goycoolea
Published 2019 · Materials Science, Medicine

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A new method for the molecular weight (MW) determination of chitosans by asymmetric flow field flow fractionation (AF-FFF or AF4) coupled with multi-angle light scattering (MALS) and differential refractive index (RI) detectors is proposed. The method allows the separation of polymer from molecular aggregates typically found in chitosan solutions making possible the accurate determination of MW distribution of the polymer. The effect of different experimental conditions on the obtained results has been evaluated and compared with those obtained from SEC-MALS-RI (size exclusion chromatography-MALS-RI). The chitosans analysed were from different biological sources and of varying degree of acetylation. AF4-MALS-RI results revealed that although aggregates are minimised when using a good solvent, a fraction still remains, and it is only completely eliminated by filtration through small pore sizes (≤0.45 μm). This represents a limitation for the characterisation of high MW chitosan, where filtration can lead to considerable sample loss in the filter. The method described here has the advantage of not only allowing the identification and separation of the aggregates present, but also the accurate determination of the MW distribution for a wide range of chitosans of varying type where results are well correlated with those obtained by more conventional techniques.
This paper references
Flow field-flow fractionation: critical overview.
Karl-Gustav Wahlund (2013)
Improved reproducibility in the determination of the molecular weight of chitosan by analytical size exclusion chromatography
Sophie Nguyen (2009)
Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells
M. Kaiser (2015)
Chitosan-alginate blended nanoparticles as carriers for the transmucosal delivery of macromolecules.
F. M. Goycoolea (2009)
Chitosan—A versatile semi-synthetic polymer in biomedical applications
M. Dash (2011)
Characterization of chitosan and its derivatives using asymmetrical flow field-flow-fractionation: a comparison with traditional methods.
S. Mao (2007)
Chitin and Chitosan Preparation from Marine Sources. Structure, Properties and Applications
I. Younes (2015)
Amylose and amylopectin in starch by asymmetric flow field-flow fractionation with multi-angle light scattering and refractive index detection (AF4–MALS–RI)
E. Chiaramonte (2012)
Programmed cross flow asymmetrical flow field-flow fractionation for the size separation of pullulans and hydroxypropyl cellulose.
Mats Leeman (2006)
Study on antimicrobial activity of chitosan with different molecular weights
lian-ying Zheng (2003)
Asymmetrical flow field-flow fractionation coupled to multiangle laser light scattering detector: optimization of crossflow rate, carrier characteristics, and injected mass in alginate separation.
E. Alasonati (2007)
Chitosan as antimicrobial agent: applications and mode of action.
E. Rabea (2003)
Effects of ionic strength on the size and compactness of chitosan nanoparticles
Helene Jonassen (2012)
Preparation and antibacterial activity of chitosan nanoparticles.
Lifeng Qi (2004)
Chitosans as Absorption Enhancers for Poorly Absorbable Drugs. 1: Influence of Molecular Weight and Degree of Acetylation on Drug Transport Across Human Intestinal Epithelial (Caco-2) Cells
N. G. Schipper (2004)
Aggregates in acidic solutions of chitosans detected by static laser light scattering
M. Anthonsen (1994)
Development and characterization of new insulin containing polysaccharide nanoparticles.
B. Sarmento (2006)
Chitosan-based hydrogels for controlled, localized drug delivery.
N. Bhattarai (2010)
Chitosan and its hydrophobic derivatives: Preparation and aggregation in dilute aqueous solutions
O. Philippova (2012)
Antimicrobial properties of chitosan and mode of action: a state of the art review.
M. Kong (2010)
Chitin and chitosan: Properties and applications
M. Rinaudo (2006)
Typical physicochemical behaviors of chitosan in aqueous solution.
C. Schatz (2003)
Antibacterial cellulose membrane via one-step covalent immobilization of ammonium/amine groups
Jianqiang Meng (2015)
Characterization of chitosan. Influence of ionic strength and degree of acetylation on chain expansion.
M. Rinaudo (1993)
Characterization of chitosan by steric exclusion chromatography
J. Brugnerotto (2001)
Optimization and evaluation of asymmetric flow field-flow fractionation of silver nanoparticles.
K. Loeschner (2013)
Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in an N-Acyl Homoserine Lactone (AHL)-Based Escherichia coli Biosensor
X. Qin (2018)
Reactivity and electrokinetical properties of different types of regenerated cellulose fibres
K. Stana-Kleinschek (2001)
Chitosan-based nanomaterials: a state-of-the-art review.
S. K. Shukla (2013)
Characterizing molar mass distributions and molecule structures of different chitosans using asymmetrical flow field-flow fractionation combined with multi-angle light scattering.
C. Augsten (2008)
Recent development of chitosan-based polyelectrolyte complexes with natural polysaccharides for drug delivery.
Y. Luo (2014)
Transfection efficiency of chitosan vectors: effect of polymer molecular weight and degree of deacetylation.
M. Huang (2005)
Asymmetric flow field-flow fractionation in the field of nanomedicine.
M. Wagner (2014)
Physical Properties and Stability of Soft Gelled Chitosan-Based Nanoparticles.
F. Goycoolea (2016)
Characterization of cationic polymers by asymmetric flow field-flow fractionation and multi-angle light scattering-A comparison with traditional techniques.
M. Wagner (2014)
Structure of chitosan determines its interactions with mucin.
B. Menchicchi (2014)
Physical properties and antibacterial activity of chitosan/acemannan mixed systems.
A. Y. Escobedo-Lozano (2015)
Chitosan in Non-Viral Gene Delivery: Role of Structure, Characterization Methods, and Insights in Cancer and Rare Diseases Therapies
Beatriz Santos-Carballal (2018)

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