Online citations, reference lists, and bibliographies.
← Back to Search

Chitosan-hyaluronic Acid Nanoparticles Loaded With Heparin For The Treatment Of Asthma.

F. Oyarzun-Ampuero, J. Brea, M. I. Loza, D. Torres, M. Alonso
Published 2009 · Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The purpose of this study was to produce mucoadhesive nanocarriers made from chitosan (CS) and hyaluronic acid (HA), and containing the macromolecular drug heparin, suitable for pulmonary delivery. For the first time, this drug was tested in ex vivo experiments performed in mast cells, in order to investigate the potential of the heparin-loaded nanocarriers in antiasthmatic therapy. CS and mixtures of HA with unfractionated or low-molecular-weight heparin (UFH and LMWH, respectively) were combined to form nanoparticles by the ionotropic gelation technique. The resulting nanoparticles loaded with UFH were between 162 and 217 nm in size, and those prepared with LMWH were 152 nm. The zeta potential of the nanoparticle formulations ranged from +28.1 to +34.6 mV, and in selected nanosystems both types of heparin were associated with a high degree of efficiency, which was approximately 70%. The nanosystems were stable in phosphate buffered saline (PBS), pH 7.4, for at least 24h, and released 10.8% of UFH and 79.7% of LMWH within 12h of incubation. Confocal microscopy experiments showed that fluorescent heparin-loaded CS-HA nanoparticles were effectively internalized by rat mast cells. Ex vivo experiments aimed at evaluating the capacity of heparin to prevent histamine release in rat mast cells indicated that the free or encapsulated drug exhibited the same dose-response behaviour.
This paper references
10.1111/j.1476-5381.1995.tb13367.x
Inhibition of serum‐induced proliferation of bovine tracheal smooth muscle cells in culture by heparin and related glycosaminoglycans
S. Kilfeather (1995)
10.1016/S0168-3659(99)00285-0
Preparation and evaluation of the in vitro drug release properties and mucoadhesion of novel microspheres of hyaluronic acid and chitosan.
S. T. Lim (2000)
10.1016/J.JCONREL.2004.07.030
Transmucosal macromolecular drug delivery.
C. Prego (2005)
10.1152/JAPPL.1998.84.1.222
Inhibition of allergic airway responses by inhaled low-molecular-weight heparins: molecular-weight dependence.
J. Martinez-Salas (1998)
10.1016/0378-5173(95)04280-6
Evaluation of the bioadhesive properties of hyaluronan derivatives: Detachment weight and mucociliary transport rate studies
K. Pritchard (1996)
10.1016/J.EJPS.2005.02.008
A comparative study of the potential of solid triglyceride nanostructures coated with chitosan or poly(ethylene glycol) as carriers for oral calcitonin delivery.
M. Garcia-Fuentes (2005)
10.1007/s11095-005-4596-x
Chitosan Nanoparticles as New Ocular Drug Delivery Systems: In Vitro Stability, in Vivo Fate, and Cellular Toxicity
A. Campos (2005)
10.1586/14760584.4.2.185
Nanoparticles as carriers for nasal vaccine delivery
M. Köping-Höggård (2005)
10.1139/y97-106
Characterization of molecular determinants of smooth muscle cell heterogeneity.
A. Halayko (1997)
10.1016/J.JACI.2004.03.034
The role of the mast cell in asthma: induction of airway hyperresponsiveness by interaction with smooth muscle?
D. Robinson (2004)
10.1016/0162-3109(81)90001-1
The effect of concentration on the binding of compound 48/80 to rat mast cells: a fluorescence microscopy study.
M. J. Ortner (1981)
10.1165/AJRCMB/8.5.518
Effect of salbutamol, fenoterol, and sodium cromoglycate on the release of heparin from sensitized human lung fragments challenged with Dermatophagoides pteronyssinus allergen.
W. Green (1993)
10.1016/S0898-6568(01)00169-3
Ouabain-induced enhancement of rat mast cells response. Modulation by protein phosphorylation and intracellular pH.
J. Lago (2001)
10.1038/gt.2008.16
Bioadhesive hyaluronan–chitosan nanoparticles can transport genes across the ocular mucosa and transfect ocular tissue
M. Fuente (2008)
10.1164/AJRCCM.157.3.9708027
Inhibition of antigen-induced airway hyperresponsiveness by ultralow molecular-weight heparin.
J. Molinari (1998)
10.1002/mabi.200700190
Novel hyaluronan-based nanocarriers for transmucosal delivery of macromolecules.
M. de la Fuente (2008)
10.1038/sj.bjp.0706333
Some structural determinants of the antiproliferative effect of heparin‐like molecules on human airway smooth muscle
V. Kanabar (2005)
10.1378/CHEST.123.4.1254
Alternate treatments in asthma.
A. Niven (2003)
10.1021/JS960182O
Chitosan as a nasal delivery system: the effect of chitosan solutions on in vitro and in vivo mucociliary transport rates in human turbinates and volunteers.
T. Aspden (1997)
10.1152/AJPLUNG.1995.269.4.L514
Heparin and PGE2 inhibit DNA synthesis in human airway smooth muscle cells in culture.
P. Johnson (1995)
10.1152/JAPPL.1999.86.2.549
Molecular-weight-dependent effects of nonanticoagulant heparins on allergic airway responses.
C. Campo (1999)
10.1016/S0006-2952(99)00378-0
Advances in immunopharmacology of asthma.
W. S. Wong (2000)
10.1016/0140-6736(91)90289-2
One explanation of the asthma paradox: inhibition of natural anti-inflammatory mechanism by β2-agonists
C. Page (1991)
10.1016/J.JCIS.2004.08.186
Physicochemical characterization of chitosan nanoparticles: electrokinetic and stability behavior.
T. López-León (2005)
10.1002/(SICI)1097-4628(19970103)63:1<125::AID-APP13>3.0.CO;2-4
Novel hydrophilic chitosan‐polyethylene oxide nanoparticles as protein carriers
P. Calvo (1997)
10.1016/j.bcp.2008.07.011
A new chemical tool (C0036E08) supports the role of adenosine A(2B) receptors in mediating human mast cell activation.
M. Buceta (2008)
10.1152/AJPLUNG.2001.281.6.L1313
Human mast cell and airway smooth muscle cell interactions: implications for asthma.
S. Page (2001)
10.1152/JAPPL.2000.88.5.1721
Inhibition of allergic late airway responses by inhaled heparin-derived oligosaccharides.
T. Ahmed (2000)



This paper is referenced by
10.1208/s12249-019-1561-2
QbD Approach for Novel Crosslinker-Free Ionotropic Gelation of Risedronate Sodium–Chitosan Nebulizable Microspheres: Optimization and Characterization
Omar A. Elkady (2019)
10.1016/J.BEJ.2015.06.017
Chitosan nanoparticles produced with the gradual temperature decrease technique for sustained gene delivery
C. C. Sipoli (2015)
10.1016/j.carbpol.2012.06.056
Preparation and evaluation of warfarin-β-cyclodextrin loaded chitosan nanoparticles for transdermal delivery.
Safaa K. H. Khalil (2012)
10.1039/C7TB03273K
The functionalization of natural polymer-coated gold nanoparticles to carry bFGF to promote tissue regeneration.
Helena Ferreira (2018)
10.1016/B978-0-08-101914-6.00010-7
Biopolymers for gene delivery applications
L. G. L. Torre (2017)
10.1039/C5TB01517K
Novel protein-loaded chondroitin sulfate-N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan nanoparticles with reverse zeta potential: preparation, characterization, and ex vivo assessment.
Tsung-Neng Tsai (2015)
10.1016/J.COLSURFA.2017.05.089
Release Kinetics from Nano-Inclusion-Based and Affinity-Based Hydrogels: A Comparative Study
Pierre-Luc Latreille (2017)
10.1038/ja.2013.32
Pulmonary disposition of vancomycin nebulized as lipid vesicles in rats
M. J. D. J. Valle (2013)
10.1016/j.ijpharm.2016.03.014
Polymeric protective agents for nanoparticles in drug delivery and targeting.
G. D. Mogoșanu (2016)
10.1016/j.ijbiomac.2019.06.031
Appliance of fungal chitosan/ceftriaxone nano-composite to strengthen and sustain their antimicrobial potentiality against drug resistant bacteria.
Fawzia A Alshubaily (2019)
10.1016/bs.apcsb.2014.11.003
Polysaccharide nanoparticles for protein and Peptide delivery: exploring less-known materials.
M. A. dos Santos (2015)
10.2174/2211738511301010035
Primaquine Loaded Chitosan Nanoparticles For Liver Targeting
N. Gupta (2012)
10.1016/j.ejpb.2015.04.006
Chondroitin-based nanoplexes as peptide delivery systems--Investigations into the self-assembly process, solid-state and extended release characteristics.
A. Umerska (2015)
10.24190/ISSN2564-615X/2017/01.09
Biological effects induced by Gadolinium nanoparticles on Lymphocyte A20 cell line
C. V. Gheran (2017)
10.1021/BK-2011-1091.CH011
Biological applications of hyaluronic acid functionalized nanomaterials
M. El-Dakdouki (2011)
10.1016/j.ijpharm.2011.04.031
Chitosan/sulfobutylether-β-cyclodextrin nanoparticles as a potential approach for ocular drug delivery.
Azza A. Mahmoud (2011)
10.3390/nano5031379
Hyaluronic Acid-Chitosan Nanoparticles to Deliver Gd-DTPA for MR Cancer Imaging
L. Zhang (2015)
10.1002/ADFM.201701219
Screening of Nanocomposite Scaffolds Arrays Using Superhydrophobic‐Wettable Micropatterns
Álvaro J Leite (2017)
10.2217/nnm.16.6
Nanoparticle drug delivery systems and their use in cardiac tissue therapy.
Y. Ho (2016)
10.1016/j.carres.2014.07.016
Polysaccharide-based nanocomposites and their applications.
Y. Zheng (2015)
10.2174/157017812799303999
Intelligent Drug Delivery Systems Based on Modified Chitosan Nanoparticles
M. Saboktakin (2012)
10.2174/1568026614666140329225817
Nanocarriers for respiratory diseases treatment: recent advances and current challenges.
A. Trapani (2014)
Development Of Glycosaminoglycan Scaffold Integrated With Herbal Nanoparticle For Wound Healing
Liji Thomas (2016)
10.1016/J.CARBPOL.2011.03.022
Microspheres loaded with polysaccharide nanoparticles for pulmonary delivery: preparation, structure and surface analysis
S. Al-Qadi (2011)
10.15386/cjmed-574
Hypersensitivity and nanoparticles: update and research trends
T. Mocan (2016)
10.1016/j.carbpol.2014.08.077
Chitosan-hyaluronic acid polyelectrolyte complex scaffold crosslinked with genipin for immobilization and controlled release of BMP-2.
S. D. Nath (2015)
For Review. Confidential - ACS
M. Gidley (2009)
10.1016/j.actbio.2010.08.028
Development of novel nanoparticles shelled with heparin for berberine delivery to treat Helicobacter pylori.
Chiung-Hung Chang (2011)
10.1007/978-81-322-2511-9_10
Chitosan: A Promising Substrate for Regenerative Medicine in Drug Formulation
Madhu Kashyap (2016)
10.1016/j.ejpb.2011.04.008
A new drug nanocarrier consisting of polyarginine and hyaluronic acid.
F. Oyarzun-Ampuero (2011)
10.1016/j.jsps.2016.12.008
Effect of cryoprotection on particle size stability and preservation of chitosan nanoparticles with and without hyaluronate or alginate coating
Abdulaziz M Almalik (2017)
10.1016/j.xphs.2019.10.007
Chitosan/sulfobutylether-β-cyclodextrin nanoparticles for ibrutinib delivery: a potential nanoformulation of novel kinase inhibitor.
L. Zhao (2019)
See more
Semantic Scholar Logo Some data provided by SemanticScholar