Please confirm you are human (Sign Up for free to never see this)
← Back to Search
Cytotoxicity Assessment Of Heparin Nanoparticles In NR8383 Macrophages.
H. Eidi, O. Joubert, G. Attik, R. E. Duval, M. Bottin, A. Hamouia, P. Maincent, B. Rihn
Published 2010 · Chemistry, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
The bioavailability of low molecular weight heparin (LMWH) has been increased by encapsulation in nanoparticles. As a complement to these results, the cytotoxicity and apoptosis induced by LMWH nanoparticles prepared by two methods [nanoprecipitation (NP) and double emulsion (DE)] using Eudragit RS (RS) and poly-epsilon-caprolactone (PCL) have been analysed. Particle sizes varied from 54 to 400nm with zeta potential values between -65 and +63mV. Our results showed that the method of nanoparticle preparation affects their properties, especially in terms of drug incorporation and cell tolerance. Cell viability ranged from 6% to 100% depending on the preparation method and physicochemical properties of the particles and the type of toxicity assay. Particle diameter and zeta potential seemed to be the most valuable cytotoxicity markers when cell viability was measured by Trypan blue exclusion and MTT respectively. Nanoparticles prepared by DE were better tolerated than those of NP. LMWH encapsulation into the cationic nanoparticles reduces remarkably their toxicity. Apoptosis evaluation showed activated caspases in exposed cells. However, no nuclear fragmentation was detected in NR8383 cells whatever the tested nanoparticles. DE nanoparticles of RS and PCL can be proposed as a good LMWH delivery system due to their low toxicity (IC(50) approximately 2.33 and 0.96mg/mL, respectively).
This paper references
Nominal and effective dosimetry of silica nanoparticles in cytotoxicity assays.
D. Lison (2008)
Eudragit RS 100 nanosuspensions for theophthalmic controlleddeliveryof ibuprofen
U. Pison (2002)
Polymer genomics: an insight into pharmacology and toxicology of nanomedicines.
A. Kabanov (2006)
Work in Brief
D. Loomis (2006)
Internalization, Cytotoxicity, Apoptosis, and Tumor Necrosis Factor-α Expression in Rat Alveolar Macrophages Exposed to Various Dusts Occurring in the Ceramics Industry
G. Attik (2008)
Nanoparticle and targeted systems for cancer therapy.
L. Brannon-Peppas (2004)
Caspases and calpain are independent mediators of cisplatin-induced endothelial cell necrosis.
B. Dursun (2006)
In vitro assessments of nanomaterial toxicity.
Clinton F Jones (2009)
Nanoparticle interaction with plasma proteins as it relates to particle biodistribution, biocompatibility and therapeutic efficacy.
P. Aggarwal (2009)
Effects of phospholipid surfactant on apoptosis induction by respirable quartz and kaolin in NR8383 rat pulmonary macrophages.
N. Gao (2001)
Disposition kinetics and oral bioavailability of vincamine-loaded polyalkyl cyanoacrylate nanoparticles.
P. Maincent (1986)
Oral bioavailability of a low molecular weight heparin using a polymeric delivery system.
V. Hoffart (2006)
Preparation and in vivo studies of a new drug delivery system
P. Maincent (1984)
In vitro investigation of ionic polysaccharide microspheres for simultaneous delivery of chemosensitizer and antineoplastic agent to multidrug-resistant cells.
Z. Liu (1999)
Internalization, cytotoxicity, apoptosis, and tumor necrosis factor-alpha expression in rat alveolar macrophages exposed to various dusts occurring in the ceramics industry.
G. Attik (2008)
Targeting to macrophages: role of physicochemical properties of particulate carriers--liposomes and microspheres--on the phagocytosis by macrophages.
F. Ahsan (2002)
Comparative toxicity of 24 manufactured nanoparticles in human alveolar epithelial and macrophage cell lines
S. Lanone (2009)
Nanoparticles: pharmacological and toxicological significance
C. Medina (2007)
Science review: Apoptosis in acute lung injury
G. Matute-Bello (2003)
Optimization of a New Non-viral Vector for Transfection: Eudragit Nanoparticles for the Delivery of a DNA Plasmid
M. Gargouri (2009)
Toxic effects of two pesticides, Imazalil and Triadimefon, on the early development of the ascidian Phallusia mammillata (Chordata, Ascidiacea).
R. Pennati (2006)
Controlled release of heparin from poly(ε-caprolactone) electrospun fibers
E. Luong-van (2006)
Current in vitro methods in nanoparticle risk assessment: limitations and challenges.
A. Kroll (2009)
Nanoparticles in Medicine: Therapeutic Applications and Developments
L. Zhang (2008)
Investiga - tion of the role of macrophages on the cytotoxicity of doxorubicin and doxorubicinloaded nanoparticles onM 5076 cells in vitro
C. Dubernet (2000)
Pseudoephedrine HCl microspheres formulated into an oral suspension dosage form
R. Bodmeier (1991)
Trimethylated chitosans as non-viral gene delivery vectors: cytotoxicity and transfection efficiency.
T. Kean (2005)
Implicationof caspases during maedivisna virusinduced apoptosis
M. errari (2002)
Characterisation of surface-modified solid lipid nanoparticles (SLN): influence of lecithin and nonionic emulsifier.
M. Schubert (2005)
Evaluation of cytotoxic effects and oxidative stress with hydroxyapatite dispersions of different physicochemical properties in rat NR8383 cells and primary macrophages.
C. Albrecht (2009)
Branched polyesters based on poly[vinyl-3-(dialkylamino)alkylcarbamate-co-vinyl acetate-co-vinyl alcohol]-graft-poly(d,l-lactide-co-glycolide): effects of polymer structure on cytotoxicity.
F. Unger (2007)
Relevance of the colloidal stability of chitosan/PLGA nanoparticles on their cytotoxicity profile.
N. Nafee (2009)
Characterization of a rat alveolar macrophage cell line that expresses a functional mannose receptor
K. Lane (1998)
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
Drug targeting with nano-sized carrier systems
M. Yokoyama (2005)
Nanomedicine for respiratory diseases.
U. Pison (2006)
In Vitro and In Vivo Evaluation of Oral Heparin–Loaded Polymeric Nanoparticles in Rabbits
Y. Jiao (2002)
Comparison of the Usefulness of the MTT, ATP, and Calcein Assays to Predict the Potency of Cytotoxic Agents in Various Human Cancer Cell Lines
H. Müller (2004)
Not ready to use – overcoming pitfalls when dispersing nanoparticles in physiological media
Christine Schulze (2008)
Nanotechnology-based drug delivery systems
S. Suri (2007)
Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles
G. Oberdörster (2005)
The Economic Impact of Treating Deep Vein Thrombosis with Low-Molecular-Weight Heparin: Outcome of Therapy and Health Economy Aspects
R. Hull (1998)
A New Polymer–Lipid Hybrid Nanoparticle System Increases Cytotoxicity of Doxorubicin Against Multidrug-Resistant Human Breast Cancer Cells
H. L. Wong (2006)
Self-assembled biodegradable micellar nanoparticles of amphiphilic and cationic block copolymer for siRNA delivery.
Tian-meng Sun (2008)
Nanotoxicity: the growing need for in vivo study.
H. C. Fischer (2007)
In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis.
D. Fischer (2003)
Comparison of two methods of encapsulation of an oligonucleotide into poly(D,L-lactic acid) particles.
F. Delié (2001)
Dosage de l ’ héparine : étude critique
G. ttik (1967)
Nanocapsule formation by interfacial polymer deposition following solvent displacement
H. Fessi (1989)
Low molecular weight heparin nanoparticles: mucoadhesion and behaviour in Caco-2 cells
A. Lamprecht (2006)
Cancer nanotechnology: opportunities and challenges. Comment (letter)
A. Nemmar (2005)
5-amino salicylic acid bound nanoparticles for the therapy of inflammatory bowel disease.
David Pertuit (2007)
Oral evaluation in rabbits of cyclosporin-loaded Eudragit RS or RL nanoparticles.
N. Ubrich (2005)
Development of microparticles prepared by spray-drying as a vaccine delivery system against brucellosis.
M. Murillo (2002)
Toxicity screening of biodegradable polymers. II. Evaluation of cell culture test with medium extract
M. Dang (1997)
Comparison of two methods of encapsulation of an oligonucleotide into poly ( d , llactic acid ) particles
K. onaldson (2001)
Investigation of the role of macrophages on the cytotoxicity of doxorubicin and doxorubicin-loaded nanoparticles on M5076 cells in vitro.
C. E. Soma (2000)
Effects of synthetic polycations on leucine incorporation, lactate dehydrogenase release, and morphology of human umbilical vein endothelial cells.
D. Morgan (1988)
Mitochondria‐mediated caspase‐independent apoptosis induced by cadmium in normal human lung cells
Chwen-Ming Shih (2003)
Effect of surface properties on nanoparticle-cell interactions.
Ayush Verma (2010)
Eudragit RS100 nanosuspensions for the ophthalmic controlled delivery of ibuprofen.
R. Pignatello (2002)
Drug targetingwith nanosized carrier systems
M. Yokoyama (2005)
Cytotoxicity of polymerized resin cements on human dental pulp cells in vitro.
Ninghua Kong (2009)
Nanomedicine for respiratory diseases.
H. Swai (2009)
Oral bioavailability of a lowmolecularweightheparinusingapolymericdelivery system
R. D. ull (2006)
Prepa - ration and in vivo studies of a new drug delivery system . Nanoparticles of alkylcyanoacrylate
P. Maincent (1984)
Delivering cancer stem cell therapies - a role for nanomedicines?
A. G. Schätzlein (2006)
Nanotechnology: Intelligent Design to Treat Complex Disease
P. Couvreur (2006)
Lowmolecu - lar weight heparin nanoparticles : mucoadhesion and behaviour in Caco2 cells
K. B. Lane (2006)
Comparison of the usefulness of theMTT , ATP , and calcein assays to predict the potency of cytotoxic agents in various human cancer cell lines
M. Murillo (2004)
Polyester Microparticles as a Vaccine Delivery System for Brucellosis: Influence of the Polymer on Release, Phagocytosis and Toxicity
M. Murillo (2002)
The use of Eudragit RS 100/cyclodextrin nanoparticles for the transmucosal administration of glutathione.
A. Lopedota (2009)
Macrophage Culture as a Suitable Paradigm for Evaluation of Synthetic Vitreous Fibers
Hermine Dika Nguea (2008)
Zeta-potential and morphology of electrospun nano- and microfibers from biopolymers and their blends used as scaffolds in tissue engineering
C. Vaquette (2008)
Recommendations for Toxicological Evaluation of Nanoparticle Medicinal Products
J. Claude (2009)
Implication of caspases during maedi-visna virus-induced apoptosis.
R. Duval (2002)
Spontaneous formation of drug-containing acrylic nanoparticles.
R. Bodmeier (1991)
Assay conditions can influence the outcome of cytotoxicity tests of nanomaterials: better assay characterization is needed to compare studies.
J. Geys (2010)
Nanoparticles - A Review
V. Mohanraj (2007)
This paper is referenced by
Cytotoxic effects of four Caryophyllaceae species extracts on macrophage cell lines
R. Gevrenova (2014)
Cannabinoids delivery systems based on supramolecular inclusion complexes and polymeric nanocapsules for treatment of neuropathic pain
F. Astruc-Diaz (2012)
Determining nanomaterials in food
C. Blasco (2011)
Nanoencapsulation of the flavonoid dihydromyricetin protects against the genotoxicity and cytotoxicity induced by cationic nanocapsules.
A. Dalcin (2019)
Abiotic dissolution rates of 24 (nano)forms of 6 substances compared to macrophage-assisted dissolution and in vivo pulmonary clearance: Grouping by biodissolution and transformation
Johanna Koltermann-Jülly (2018)
INVESTIGATION OF IN VITRO CYTOTOXIC EFFECTS OF HEPARIN COATED IRON OXIDE NANOPARTICLES COMBINED WITH TPP-DCA ON HUMAN HEPATOCELLULAR CARCINOMA CELL LINE HEPG
Halil Kalıpçılar (2018)
Nanoprecipitation Process: From Particle Preparation to In Vivo Applications
Karim Miladi (2016)
Investigating the Skin Penetration and Wound Healing Properties of Niosomal Pentoxifylline Cream.
A. Aghajani (2020)
Cytotoxicity and genotoxicity of lipid nanocapsules.
G. Le Roux (2017)
Polymeric protective agents for nanoparticles in drug delivery and targeting.
G. D. Mogoșanu (2016)
Safety assessment of nanoparticles for drug delivery by means of classic in vitro assays and beyond
Stefan Lorscheidt (2016)
Biodegradable thermogelling poly(ester urethane)s consisting of poly(1,4-butylene adipate), poly(ethylene glycol), and poly(propylene glycol)
Chengde Liu (2013)
Chapter 7 Nanoparticle-based vaccines opportunities and limitations
D. Diaz-Arévalo (2020)
Viability and gene expression responses to polymeric nanoparticles in human and rat cells
C. Ronzani (2014)
In vivo toxicity of enoxaparin encapsulated in mucoadhesive nanoparticles: Topical application in a wound healing model
S. C. Huber (2013)
Ocular Application of Dirithromycin Incorporated Polymeric Nanoparticles: an In Vitro Evaluation
E. Başaran (2017)
Unique growth pattern of human mammary epithelial cells induced by polymeric nanoparticles
R. Hussien (2013)
THESE DE L'UNIVERSITE DE LYON Délivrée par L'UNIVERSITE CLAUDE BERNARD LYON 1
École Doctorale (2010)
Particles from preformed polymers as carriers for drug delivery
K. Miladi (2014)
Nanomaterials in food, which way forward?
Y. Picó (2012)
Uptake of Eudragit Retard L (Eudragit® RL) Nanoparticles by Human THP-1 Cell Line and Its Effects on Hematology and Erythrocyte Damage in Rats
M. Abdel-Wahhab (2014)
Development and effect of storage on the stability of enriched flavonoid fraction of Cecropia glaziovii-loaded PLGA nanoparticles
T. Santos (2016)
Nanoparticles in Foods, Determination of
C. Blasco (2013)
Nano systems for drug delivery: Which gap in preclinical studies?
B. Rihn (2011)
Heparin-conjugated scaffolds with pore structure of inverted colloidal crystals for cartilage regeneration.
Y. Kuo (2011)
Characterization of Antimicrobial Agent Loaded Eudragit RS Solvent Exchange-Induced In Situ Forming Gels for Periodontitis Treatment
T. Phaechamud (2016)
Engineered nanoparticles: thrombotic events in cancer.
A. Abdalla (2014)
Chlorhexidine Nanocapsule Drug Delivery Approach to the Resin-Dentin Interface
B. Priyadarshini (2016)
Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation
Chung-King Hsu (2011)
Drug delivery by polymeric nanoparticles induces autophagy in macrophages.
H. Eidi (2012)
Étude toxicologique de nanoparticules polymériques véhicules de S-nitrosoglutathion
Ramia Safar (2015)
Fluorescent nanodiamonds as a relevant tag for the assessment of alum adjuvant particle biodisposition
H. Eidi (2015)See more