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Noninvasive And Persistent Transfollicular Drug Delivery System Using A Combination Of Liposomes And Iontophoresis.
K. Kajimoto, M. Yamamoto, Misuzu Watanabe, K. Kigasawa, K. Kanamura, H. Harashima, K. Kogure
Published 2011 · Medicine
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Iontophoresis is a promising technique for enhancing transdermal administration of charged drugs. However, conventional iontophoresis is not sufficient for effective delivery of large, hydrophilic, or electrically neutral molecules. In this study, we utilized charged liposomes as carriers, focused on a transfollicular route for delivery of the liposomes, and optimized iontophoretic conditions and lipid composition for this method in both in vitro and in vivo conditions. As a result, we identified the optimum condition (lipid composition: DOTAP/EPC/Chol=2:2:1, current supply: 0.45mA/cm(2), duration: 1h) for effective iontophoretic delivery of aqueous solution, which cannot be transferred into the skin without charged liposomes. We also examined the pharmacological effects of iontophoresis of liposomes encapsulating insulin (INS-lipo) using a rat model of type I diabetes. Interestingly, iontophoresis of INS-lipo onto a diabetes rat skin resulted in a gradual decrease in blood glucose levels, with levels reaching 20% of initial values at 18h after administration. These lower blood glucose levels were maintained for up to 24h. Significant amount of insulin were also detected in plasma 18h after iontophoresis of INS-lipo. We succeeded in developing a non-invasive and persistent transfollicular drug delivery system that used a combination of liposomes and iontophoresis.
This paper references
Site‐specific methylene blue delivery to pilosebaceous structures using highly porous nylon microspheres: An experimental evaluation
S. Mordon (2003)
Central Responsiveness to a Ghrelin Mimetic (GHRP‐6) is Rapidly Altered by Acute Changes in Nutritional Status in Rats
Y. C. Tung (2005)
Perifollicular transgenic expression of human interleukin-1 receptor antagonist protein following topical application of novel liposome-plasmid DNA formulations in vivo.
S. Niemiec (1997)
Pilosebaceous unit: anatomical considerations and drug delivery opportunities
Paramjit Singh (2000)
Enhanced transfollicular delivery of adriamycin with a liposome and iontophoresis
I. Han (2004)
Enhanced skin permeation of diclofenac by iontophoresis: in vitro and in vivo studies
Elizabeth Varghese (1996)
The pilosebaceous unit: a pivotal route for topical drug delivery.
R. Agarwal (2000)
excised human-skin during iontophoresis
C. J. Chang (1998)
Topical delivery of growth hormone releasing peptide using liposomal systems: an in vitro study using hairless mouse skin.
D. Fleisher (1995)
Penetration profile of microspheres in follicular targeting of terminal hair follicles.
R. Toll (2004)
Transdermal drug delivery
M. Prausnitz (2008)
Inducing effect of liposomalization on the transdermal delivery of hydrocortisone: creation of a drug supersaturated state.
J. Barichello (2006)
Site-Specific Drug Delivery to Pilosebaceous Structures Using Polymeric Microspheres
A. Rolland (2004)
Skin biological issues in electrically enhanced transdermal delivery
P. Ledger (1992)
Expression pattern and intensity of protoporphyrin IX induced by liposomal 5-aminolevulinic acid in rat pilosebaceous unit throughout hair cycle
I. Han (2005)
Electroosmosis in transdermal iontophoresis: implications for noninvasive and calibration-free glucose monitoring.
A. Sieg (2004)
Characterization of Streptozotocin-induced Diabetic Rats and Pharmacodynamics of Insulin Formulations
Jae-jeong Lee (2003)
Transfollicular drug delivery--is it a reality?
V. Meidan (2005)
Sympathetic hyperactivity in Wistar rats with insulin-resistance.
C. Chang (1998)
Transfer and exchange of phospholipid between small unilamellar liposomes and rat plasma high density lipoproteins. Dependence on cholesterol content and phospholipid composition.
J. Damen (1981)
New model of progressive non-insulin-dependent diabetes mellitus in mice induced by streptozotocin.
M. Ito (1999)
Noninvasive delivery of siRNA into the epidermis by iontophoresis using an atopic dermatitis-like model rat.
K. Kigasawa (2010)
In vivo transdermal delivery of diclofenac by ion-exchange iontophoresis with geraniol.
K. Kigasawa (2009)
Effect of the cholesterol content of small unilamellar liposomes on their stability in vivo and in vitro.
C. Kirby (1980)
Steric stabilization of fusogenic liposomes by a low-pH sensitive PEG--diortho ester--lipid conjugate.
X. Guo (2001)
How deep do intact liposomes penetrate into human skin
J. Lasch (1992)
Skin penetration and distribution of polymeric nanoparticles.
R. Alvarez-Román (2004)
Characterization of the permselective properties of excised human skin during iontophoresis.
R. Burnette (1987)
Insulin degradation: progress and potential.
W. Duckworth (1998)
Trypsin‐induced follicular papilla apoptosis results in delayed hair growth and pigmentation
M. Seiberg (1997)
lontophoretic Devices for Drug Delivery
P. Tyle (2004)
Protein measurement using bicinchoninic acid: elimination of interfering substances.
R. Brown (1989)
Influence of Nonionic Liposomal Composition on Topical Delivery of Peptide Drugs into Pilosebaceous Units: An in Vivo Study Using the Hamster Ear Model
S. Niemiec (2004)
The role of electroosmotic flow in transdermal iontophoresis.
M. J. Pikal (1992)
Transdermal iontophoretic delivery of bovine insulin and monomeric human insulin analogue.
N. Kanikkannan (1999)
liposomes on their stability invivo and invitro
J. Lasch (1992)
hairless mouse skin
X. Guo (2001)
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Mahadi Hasan (2020)
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C. A. Oliveira (2020)
Nanoparticles Targeting STATs in Cancer Therapy
M. Ashrafizadeh (2019)
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C. P. Jha (2017)
Optimized skin optical clearing for Optical Coherence Tomography monitoring of encapsulated drug delivery through the hair follicles.
S. Zaytsev (2020)
Nanovesicles for Nanomedicine: Theory and Practices.
Sheetu Wadhwa (2019)
Interactions of Dendritic Nanomaterials with Skin Layers and Their Applications in Topical Drug Delivery
Y. Yang (2013)
Effects of liposomes with polyisoprenoids, potential drug carriers, on the cardiovascular and excretory system in rats.
O. Gawrys (2014)
Transdermal Iontophoresis – Delivery Control by Ion-Exchange Fibers and Nanocarriers
Dean Jouni Hirvonen (2016)
Non-invasive delivery of biological macromolecular drugs into the skin by iontophoresis and its application to psoriasis treatment.
T. Fukuta (2020)
Dendron‐Based Micelles for Topical Delivery of Endoxifen: A Potential Chemo‐Preventive Medicine for Breast Cancer
Y. Yang (2014)
Conception d'un patch transdermique intelligent pour le monitoring et l'aide à la prise de médicament
Y. Talbi (2018)
Increased microneedle-mediated transdermal delivery of tetramethylpyrazine to the brain, combined with borneol and iontophoresis, for MCAO prevention.
Sirui Xiao (2019)
Efficient intradermal delivery of superoxide dismutase using a combination of liposomes and iontophoresis for protection against UV-induced skin damage.
K. Kigasawa (2012)
Insulin administration: present strategies and future directions for a noninvasive (possibly more physiological) delivery
E. Matteucci (2015)
Enhanced Iontophoretic Delivery of Magnesium Ascorbyl 2-Phosphate and Sodium Fluorescein to Hairless and Hairy Mouse Skin
Min Young Kang (2012)
UNIVERSIDADE FEDERAL DE GOIÁS FACULDADE DE FARMÁCIA PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS FARMACÊUTICAS
L. Antônio (2013)
Advances in transdermal insulin delivery.
Y. Zhang (2018)
Transdermal delivery of vancomycin hydrochloride using combination of nano-ethosomes and iontophoresis: in vitro and in vivo study
Magdy I Mohammed (2016)
Protective effects of topical application of a poorly soluble antioxidant astaxanthin liposomal formulation on ultraviolet-induced skin damage.
S. Hama (2012)
Non-invasive drug delivery technology: development and current status of transdermal drug delivery devices, techniques and biomedical applications
Naseem Akhtar (2020)
Recent Advances in the Transdermal Delivery of Protein Therapeutics with a Combinatorial System of Chemical Adjuvants and Physical Penetration Enhancements
Young-Hyeon An (2020)
Peptide and Protein-Based Therapeutic Agents
Mary Joseph (2017)
Anti-cancer vaccination by transdermal delivery of antigen peptide-loaded nanogels via iontophoresis.
Mao Toyoda (2015)
Emerging micro- and nanotechnology based synthetic approaches for insulin delivery.
Ran Mo (2014)
Electric Stimulus Opens Intercellular Spaces in Skin*
S. Hama (2013)
Liposomal delivery of proteins and peptides
Janani Swaminathan (2012)
Superoxide Dismutase-Loaded Solid Lipid Nanoparticles Prepared by Cold Homogenization Method: Characterization and Permeation Study Through Burned Rat Skin
M. Karami (2016)
Effective Skin Cancer Treatment by Topical Co-delivery of Curcumin and STAT3 siRNA Using Cationic Liposomes
A. Jose (2017)
Synergistic antioxidative effect of astaxanthin and tocotrienol by co-encapsulated in liposomes
Chihiro Kamezaki (2016)
Promotion of the transdermal delivery of protein drugs by N-trimethyl chitosan nanoparticles combined with polypropylene electret
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