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Folate-Chitosan Nanoparticles Triggered Insulin Cellular Uptake And Improved In Vivo Hypoglycemic Activity.

E. El-Leithy, H. M. Abdel-Bar, Raghda Abdel-Moneum
Published 2019 · Chemistry, Medicine

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The aim of this study was to prove a prolonged control of glucose levels, in rats, by the oral use of insulinfolate-chitosan nanoparticles (FA-CS NPs).It was possible to prepare positively charged NPs with an average particle sizeof 288±5.11 nmand > 80% entrapment efficiency. The system was able to enhance the stability of insulin in presence of GIT enzymes, with less than 10% release at pH 1.2 and an 8 hr released amount of 38.92 ±4.52% in PBS pH 7.4. A 5 fold enhancement in insulin intestinal permeability and cellular uptake over insulin solution was proven. The cellular uptake pathways was found to occur by several mechanisms.Besides, cell compatibility and absence of histopathological alterations was also demonstrated. Finally, acontrolled blood glucose level for 8h in rats. These results anticipated FA-CS NPs as a promising oral insulin candidate.
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