← Back to Search
Folate-Chitosan Nanoparticles Triggered Insulin Cellular Uptake And Improved In Vivo Hypoglycemic Activity.
E. El-Leithy, H. M. Abdel-Bar, Raghda Abdel-Moneum
Published 2019 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
The aim of this study was to prove a prolonged control of glucose levels, in rats, by the oral use of insulinfolate-chitosan nanoparticles (FA-CS NPs).It was possible to prepare positively charged NPs with an average particle sizeof 288±5.11 nmand > 80% entrapment efficiency. The system was able to enhance the stability of insulin in presence of GIT enzymes, with less than 10% release at pH 1.2 and an 8 hr released amount of 38.92 ±4.52% in PBS pH 7.4. A 5 fold enhancement in insulin intestinal permeability and cellular uptake over insulin solution was proven. The cellular uptake pathways was found to occur by several mechanisms.Besides, cell compatibility and absence of histopathological alterations was also demonstrated. Finally, acontrolled blood glucose level for 8h in rats. These results anticipated FA-CS NPs as a promising oral insulin candidate.
This paper references
Protease inhibition and absorption enhancement by functional nanoparticles for effective oral insulin delivery.
Fang-Yi Su (2012)
Oral insulin delivery, the challenge to increase insulin bioavailability: Influence of surface charge in nanoparticle system
Elodie Czuba (2018)
Bioavailability enhancement, Caco-2 cells uptake and intestinal transport of orally administered lopinavir-loaded PLGA nanoparticles
G. Joshi (2016)
Preparation, characterization, and potential application of chitosan, chitosan derivatives, and chitosan metal nanoparticles in pharmaceutical drug delivery
T. Ahmed (2016)
Endocytic pathways of optimized resveratrol cubosomes capturing into human hepatoma cells.
H. M. Abdel-Bar (2017)
Cellular uptake, intracellular trafficking, and cytotoxicity of nanomaterials.
F. Zhao (2011)
Regulation of intestinal permeability: The role of proteases
H. Van Spaendonk (2017)
A shell-crosslinked polymeric micelle system for pH/redox dual stimuli-triggered DOX on-demand release and enhanced antitumor activity.
L. Wang (2017)
Oral insulin delivery: existing barriers and current counter‐strategies
Ahmed Gedawy (2018)
Efficient mucus permeation and tight junction opening by dissociable "mucus-inert" agent coated trimethyl chitosan nanoparticles for oral insulin delivery.
Mengshuai Liu (2016)
Molecular aspects of muco- and bioadhesion: tethered structures and site-specific surfaces.
Y. Huang (2000)
Short- and Longterm Glycemic Control of Streptozotocin-Induced Diabetic Rats Using Different Insulin Preparations
G. Luippold (2016)
Improved mucoadhesion and cell uptake of chitosan and chitosan oligosaccharide surface-modified polymer nanoparticles for mucosal delivery of proteins
Sathish Dyawanapelly (2016)
Chitosan nanoparticles for tamoxifen delivery and cytotoxicity to MCF-7 and Vero cells
N. R. Ravikumara (2011)
Nanocomplexes of an insulinotropic drug: optimization, microparticle formation, and antidiabetic activity in rats
Enas M. Elmowafy (2014)
Modified nanoparticles with cell-penetrating peptide and amphipathic chitosan derivative for enhanced oral colon absorption of insulin: preparation and evaluation
F. Guo (2016)
Role of Heparan Sulfate Proteoglycans in the Uptake and Degradation of Tissue Factor Pathway Inhibitor-Coagulation Factor Xa Complexes*
G. Ho (1997)
Oral insulin delivery by means of solid lipid nanoparticles
B. Sarmento (2007)
pH-sensitive non-phospholipid vesicle and macrophage-like cells: binding, uptake and endocytotic pathway.
L. Di Marzio (2008)
Synthesis and characterization of folate conjugated chitosan and cellular uptake of its nanoparticles in HT-29 cells.
P. Li (2011)
Synthesis and evaluation of lauryl succinyl chitosan particles towards oral insulin delivery and absorption.
M. Rekha (2009)
Novel hydrophilic chitosan‐polyethylene oxide nanoparticles as protein carriers
P. Calvo (1997)
Lipid nanocarriers improve paclitaxel transport throughout human intestinal epithelial cells by using vesicle-mediated transcytosis.
E. Roger (2009)
Mechanism study of cellular uptake and tight junction opening mediated by goblet cell-specific trimethyl chitosan nanoparticles.
J. Zhang (2014)
Intermolecular interactions and morphology of aqueous polymer/surfactant mixtures containing cationic chitosan and nonionic sorbitan esters.
J. Grant (2008)
Synthesis of Chitosan Succinate and Chitosan Phthalate and Their Evaluation as Suggested Matrices in Orally Administered, Colon‐Specific Drug Delivery Systems
K. Aiedeh (1999)
Synthesis and cellular uptake of folic acid-conjugated cellulose nanocrystals for cancer targeting.
Shuping Dong (2014)
Mechanisms of cellular uptake, intracellular transportation, and degradation of CIGB-300, a Tat-conjugated peptide, in tumor cell lines.
Fernando Benavent Acero (2014)
Functional nanoparticles exploit the bile acid pathway to overcome multiple barriers of the intestinal epithelium for oral insulin delivery.
W. Fan (2018)
Potential of insulin nanoparticle formulations for oral delivery and diabetes treatment
Chun Y Wong (2017)
Protective role of Mangifera indica, Cucumis melo and Citrullus vulgaris peel extracts in chemically induced hypothyroidism.
H. S. Parmar (2009)
Coordinated protein sorting, targeting and distribution in polarized cells
I. Mellman (2008)
Chitosan functionalized nanocochleates for enhanced oral absorption of cyclosporine A
Min Liu (2017)
Carboxyethylester-polyrotaxanes as a new calcium chelating polymer: synthesis, calcium binding and mechanism of trypsin inhibition.
T. Ooya (2002)
Preparation and immunological effectiveness of a swine influenza DNA vaccine encapsulated in chitosan nanoparticles.
K. Zhao (2011)
Preparation, evaluation and optimization of nanoparticles composed of thiolated triethyl chitosan: A potential approach for buccal delivery of insulin
Marzieh Rahbarian (2018)
Mechanisms regulating intestinal barrier integrity and its pathological implications
Chaithanya Chelakkot (2018)
Stimuli‐responsive delivery of therapeutics for diabetes treatment
J. Yu (2016)
Uniform Core–Shell Nanoparticles with Thiolated Hyaluronic Acid Coating to Enhance Oral Delivery of Insulin
Houkuan Tian (2018)
Beneficial properties for insulin absorption using superporous hydrogel containing interpenetrating polymer network as oral delivery vehicles.
Lichen Yin (2008)
Folic acid functionalized nanoparticles for enhanced oral drug delivery.
Emilie Roger (2012)
Nanoparticulate systems for nasal delivery of drugs: a real improvement over simple systems?
L. Illum (2007)
Design of folic acid-conjugated nanoparticles for drug targeting.
B. Stella (2000)
Design and evaluation of itraconazole loaded solid lipid nanoparticulate system for improving the antifungal therapy.
S. Mukherjee (2009)
Correction: Folate appended chitosan nanoparticles augment the stability, bioavailability and efficacy of insulin in diabetic rats following oral administration
A. Agrawal (2015)
Interactions between bioadhesive poly(acrylic acid) and calciumions
B. Kriwet (1996)
Development and validation of a rapid reversed-phase HPLC method for the determination of insulin from nanoparticulate systems.
B. Sarmento (2006)
Overviews on the cellular uptake mechanism of polysaccharide colloidal nanoparticles
S. Salatin (2017)
Elucidating the signaling mechanism of an epithelial tight-junction opening induced by chitosan.
Li-Wen Hsu (2012)
Improved stability and antidiabetic potential of insulin containing folic acid functionalized polymer stabilized multilayered liposomes following oral administration.
A. Agrawal (2014)
This paper is referenced by
Natural polysaccharides for controlled delivery of oral therapeutics: a recent update.
Buddhadev Layek (2020)
Enhanced oral delivery of insulin via the colon-targeted nanocomposite system of organoclay/glycol chitosan/Eudragit®S100
S. Lee (2020)
Can nanoparticles and nano‒protein interactions bring a bright future for insulin delivery?
T. Zhang (2020)