Colorimetric Detection Of Eukaryotic Gene Expression With DNA-derivatized Gold Nanoparticles.
Pedro Viana Baptista, Gonçalo Doria, David Henriques, Eulália Pereira, Ricardo Franco
Published 2005 · Biology, Medicine
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Thiol-linked DNA-gold nanoparticles were used in a novel colorimetric method to detect the presence of specific mRNA from a total RNA extract of yeast cells. The method allowed detection of expression of the FSY1 gene that encodes a specific fructose/H+ symporter in Saccharomyces bayanus PYCC 4565. FSY1 is strongly expressed when the yeast is grown in fructose as the sole carbon source, while cells cultivated in glucose as the sole carbon source repress gene expression. The presence of FSY1 mRNA is detected based on color change of a sample containing total RNA extracted from the organism and gold nanoparticles derivatized with a 15-mer of complementary single stranded DNA upon addition of NaCl. If FSY1 mRNA is present, the solution remains pink, changing to blue-purple in the absence of FSY1 mRNA. Direct detection of specific expression was possible from only 0.3 microg of unamplified total RNA without any further enhancement. This novel method is inexpensive, very easy to perform as no amplification or signal enhancement steps are necessary and takes less than 15 min to develop after total RNA extraction. No temperature control is necessary and color change can be easily detected visually.
This paper references
Adsorption and surface-enhanced Raman of dyes on silver and gold sols
Plato C. Lee (1982)
Ribosomal protein gene transcription in Saccharomyces cerevisiae shows a biphasic response to nutritional changes.
Gerard Griffioen (1996)
FSY1, a novel gene encoding a specific fructose/H(+) symporter in the type strain of Saccharomyces carlsbergensis.
Paula Gonçalves (2000)
Char - acterization of 16 novel human genes showing high similarity to yeast sequences
J. J. Storhoff
Differential regulation by glucose and fructose of a gene encoding a specific fructose/H+ symporter in Saccharomyces sensu stricto yeasts.
Helena Rodrigues de Sousa (2004)
Updated catalogue of homologues to human disease-related proteins in the yeast genome.
Miguel A. Andrade (1998)
Functional genomics: lessons from yeast.
Stephen G. Oliver (2002)
Yeast as a Model Organism
David Botstein (1997)
Rapid aggregation of gold nanoparticles induced by non-cross-linking DNA hybridization.
Ken Sato (2003)
Gold nanoparticle probe-based gene expression analysis with unamplified total human RNA.
Martin Huber (2004)
Homogeneous detection of unamplified genomic DNA sequences based on colorimetric scatter of gold nanoparticle probes
James J Storhoff (2004)
Characterization of 16 novel human genes showing high similarity to yeast sequences.
Fabio Stanchi (2001)
Nonspecific and Thiol-Specific Binding of DNA to Gold Nanoparticles
Pär Sandström (2003)
One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes
James J. Storhoff (1998)
Sequence-Dependent Stability of DNA-Modified Gold Nanoparticles
James J. Storhoff (2002)
Optical absorption of small metallic particles
Shigetaka Tomiya (1989)
Quantitative RT-PCR: pitfalls and potential.
Willard M. Freeman (1999)
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