Online citations, reference lists, and bibliographies.
← Back to Search

Design Of Iron Oxide Nanoparticles With Different Sizes And Surface Charges For Simple And Efficient Labeling Of Mesenchymal Stem Cells.

Jun Ichiro Jo, I. Aoki, Y. Tabata
Published 2010 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
The objective of this study is to prepare iron oxide nanoparticles with different sizes and surface potentials and evaluate the labeling efficiency of bone marrow-derived mesenchymal stem cells (MSC). The ferric and ferrous ions were co-precipitated in the presence of pullulan or the cationized and anionized derivatives to prepare iron oxide-pullulan nanoparticles of a contrast agent of magnetic resonance imaging (MRI). The size and surface potential of iron oxide-pullulan nanoparticles were changed by altering the mixing molar ratio of pullulan OH groups to ferric ions and the mixing percentage of pullulan derivatives, respectively. When MSC were labeled with the iron oxide-pullulan nanoparticles by the co-culture for 1h, the labeling efficiency and (1)H MRI relaxivity were greatly influenced by the particle size and surface potential, while the labeling procedure did not affect the viability and differentiation ability of MSC. These findings indicate that iron oxide-pullulan nanoparticle is a promising tool for the MRI labeling of MSC.
This paper references
Non-viral gene transfection technologies for genetic engineering of stem cells.
Jun-ichiro Jo (2008)
Tissue-engineered trachea from sheep marrow stromal cells with transforming growth factor beta2 released from biodegradable microspheres in a nude rat recipient.
Koji Kojima (2004)
A novel approach to therapeutic angiogenesis for patients with critical limb ischemia by sustained release of basic fibroblast growth factor using biodegradable gelatin hydrogel: an initial report of the phase I-IIa study.
A. Marui (2007)
Cardiomyocytes can be generated from marrow stromal cells in vitro.
S. Makino (1999)
Determination of reducing sugars and carbohydrates
J. Hodge (1962)
111 Inlabeled CD 34 + hematopoietic progenitor cells in a rat myocardial infarction model
S. Massoudi (2004)
Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats
C. Maniatopoulos (2004)
Preliminary in vitro study of ultrasound sonoporation cell labeling with superparamagnetic iron oxide particles for MRI cell tracking
Runyang Mo (2008)
Methods for magnetically labeling stem and other cells for detection by in vivo magnetic resonance imaging.
J. Frank (2004)
Magnetic labeling and cell sorting.
C. Owen (1984)
Multilineage potential of adult human mesenchymal stem cells.
M. Pittenger (1999)
Poly(N,N-dimethylacrylamide)-coated maghemite nanoparticles for stem cell labeling.
M. Babič (2009)
Imaging of intestinal lymphocyte homing by means of pinhole SPECT in a TNBS colitis mouse model.
R. Bennink (2004)
Cell labeling for magnetic resonance imaging with the T1 agent manganese chloride
I. Aoki (2006)
Molecular optical imaging: Applications leading to the development of present day therapeutics
K. Shah (2011)
Human Mesenchymal Stem Cells Differentiate to a Cardiomyocyte Phenotype in the Adult Murine Heart
C. Toma (2002)
Reversing the silencing of reporter sodium/iodide symporter transgene for stem cell tracking.
Y. H. Kim (2005)
Neuroprotective effect of melatonin on experimental peripheral nerve injury: an electron microscopic and biochemical study.
E. Dağlioğlu (2009)
Non-invasive in vivo optical imaging of the lacZ and luc gene expression in mice
V. Josserand (2007)
Fluorimetric DNA assay for cell growth estimation.
J. Rao (1992)
Neuronal differentiation potential of human adipose-derived mesenchymal stem cells.
E. Anghileri (2008)
DNA transfection mediated by cationic liposomes containing lipopolylysine: characterization and mechanism of action.
X. Zhou (1994)
Origin of endothelial progenitors in human postnatal bone marrow.
M. Reyes (2002)
Surface properties of superparamagnetic iron oxide MR contrast agents: ferumoxides, ferumoxtran, ferumoxsil.
C. W. Jung (1995)
Updates on stem cells and their applications in regenerative medicine
S. Bajada (2008)
Recent advances in iron oxide nanocrystal technology for medical imaging.
C. Corot (2006)
Preparation of Cationized Polysaccharides as Gene Transfection Carrier for Bone Marrow-Derived Mesenchymal Stem Cells
Jun-ichiro Jo (2010)
Ex vivo cell labeling with 64Cu–pyruvaldehyde-bis(N4-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography
Nora Adonai (2002)
Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord
P. Jendelová (2004)
Efficient labeling of mesenchymal stem cells using cell permeable magnetic nanoparticles.
J. S. Suh (2009)
Use of fluorescent quantum dot bioconjugates for cellular imaging of immune cells, cell organelle labeling, and nanomedicine: surface modification regulates biological function, including cytotoxicity
A. Hoshino (2007)
Functional characterization of mesenchymal stem cells labeled with a novel PVP-coated superparamagnetic iron oxide.
A. M. Reddy (2009)
Transplantation of Mesenchymal Stem Cells Improves Cardiac Function in a Rat Model of Dilated Cardiomyopathy
N. Nagaya (2005)
111In-labeled CD34+ hematopoietic progenitor cells in a rat myocardial infarction model.
W. Brenner (2004)
Accelerated stem cell labeling with ferucarbotran and protamine
Daniel Golovko (2009)
Enhancement of angiogenesis by the implantation of self bone marrow cells in a rat ischemic heart model.
T. Kobayashi (2000)
Novel collagen sponge reinforced with polyglycolic acid fiber produces robust, normal hair in murine hair reconstitution model.
M. Itoh (2004)
Implantation of Bone Marrow Mononuclear Cells Into Ischemic Myocardium Enhances Collateral Perfusion and Regional Function via Side Supply of Angioblasts, Angiogenic Ligands, and Cytokines
H. Kamihata (2001)
Imaging angiogenesis.
L. Dobrucki (2007)
Relaxation effects of ferucarbotran‐labeled mesenchymal stem cells at 1.5T and 3T: Discrimination of viable from lysed cells
T. Henning (2009)
Neovascularization of ischemic myocardium by human bone-marrow–derived angioblasts prevents cardiomyocyte apoptosis, reduces remodeling and improves cardiac function
A. Kocher (2001)
Nonviral vectors in the new millennium: delivery barriers in gene transfer.
M. Nishikawa (2001)
Rapid and efficient cell labeling with a MRI contrast agent by electroporation in the presence of protamine sulfate.
L. Wang (2009)
Marrow stromal cells for cellular cardiomyoplasty: feasibility and potential clinical advantages.
J. S. Wang (2000)
Development of chitosan-conjugated magnetite for magnetic cell separation
H. Honda (1998)
Characterization of aqueous dispersions of Fe(3)O(4) nanoparticles and their biomedical applications.
Fong-Yu Cheng (2005)
The preparation of magnetic nanoparticles for applications in biomedicine
P. Tartaj (2003)
Controlled release by biodegradable hydrogels enhances the ectopic bone formation of bone morphogenetic protein.
M. Yamamoto (2003)
Morphological and distribution characteristics of sweat glands in hypertrophic scar and their possible effects on sweat gland regeneration
FuXiao-Bing (2005)
Early histological evaluation of bone defect healing with and without guided bone regeneration techniques: experimental animal studies.
K. Lysiak-Drwal (2008)
MRI detection of single particles for cellular imaging.
E. Shapiro (2004)
Comparison of radiolabeled nucleoside probes (FIAU, FHBG, and FHPG) for PET imaging of HSV1-tk gene expression.
J. Tjuvajev (2002)
Effects of iron oxide incorporation for long term cell tracking on MSC differentiation in vitro and in vivo.
E. Farrell (2008)
Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor.
Y. Tabata (1999)
Skeletal Myogenic Differentiation of Mesenchymal Stem Cells Isolated from Human Umbilical Cord Blood
E. J. Gang (2004)
Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging.
A. Arbab (2003)
Transferrin receptor upregulation: in vitro labeling of rat mesenchymal stem cells with superparamagnetic iron oxide.
R. Schaefer (2007)
In vivo bioluminescence imaging.
A. Sato (2004)
Mesenchymal and neural stem cells labeled with HEDP-coated SPIO nanoparticles: In vitro characterization and migration potential in rat brain
Gaëtan J. -R. Delcroix (2009)
Neovascularization effect of biodegradable gelatin microspheres incorporating basic fi broblast growth factor , Journal of Biomaterials Science
S. Hijikata (1999)
Technology Insight: in vivo cell tracking by use of MRI
Walter J Rogers (2006)

This paper is referenced by
The effect of mechanical properties of iron oxide nanoparticle-loaded functional nano-carrier on tumor targeting and imaging.
W. I. Choi (2012)
Histological study of the biodynamics of iron oxide nanoparticles with different diameters
K. Tsuchiya (2011)
Long-term tracking of cells using inorganic nanoparticles as contrast agents: are we there yet?
A. Taylor (2012)
Co-precipitation of DEAE-dextran coated SPIONs: how synthesis conditions affect particle properties, stem cell labelling and MR contrast.
M. Barrow (2016)
Diagnostic Tools in the Evaluation and Management of Penile Cancer
Pranav Sharma (2017)
Assembly of Plasmonic and Magnetic Nanoparticles with Fluorescent Silica Shell Layer for Tri-functional SERS-Magnetic-Fluorescence Probes and Its Bioapplications
Hyung-Mo Kim (2018)
Assessment of functionalized iron oxide nanoparticles in vitro: introduction to integrated nanoimpact index
Narges Bayat (2015)
Pullulan based stimuli responsive and sub cellular targeted nanoplatforms for biomedical application: Synthesis, nanoformulations and toxicological perspective.
Ruchira Raychaudhuri (2020)
Tailoring the surface charge of dextran-based polymer coated SPIONs for modulated stem cell uptake and MRI contrast.
M. Barrow (2015)
Magnetic labeling of natural lipid encapsulations with iron-based nanoparticles
Dewen Ye (2018)
A novel blood-pooling MR contrast agent: Carboxymethyl-diethylaminoethyl dextran magnetite.
A. Sonoda (2016)
Comparison of Behaviour in Different Liquids and in Cells of Gold Nanorods and Spherical Nanoparticles Modified by Linear Polyethyleneimine and Bovine Serum Albumin
I. Pyshnaya (2014)
Superparamagnetic Iron Oxide Nanoparticles as MRI contrast agents for Non-invasive Stem Cell Labeling and Tracking
L. Li (2013)
Biocompatibility assessment of sub-5 nm silica-coated superparamagnetic iron oxide nanoparticles in human stem cells and in mice for potential application in nanomedicine.
M. Ledda (2020)
Mesenchymal Stem Cells as Vehicles for Targeted Therapies
Gabriele Putz Todd (2011)
Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells
Y. Hou (2013)
A biotechnological perspective on the application of iron oxide magnetic colloids modified with polysaccharides.
A. S. Dias (2011)
Neural Induction Potential and MRI of ADSCs Labeled Cationic Superparamagnetic Iron Oxide Nanoparticle In Vitro
Weiqiong Ma (2018)
Observation of Positively Charged Magnetic Nanoparticles Inside HepG2 Spheroids Using Electron Microscopy.
Y. Miyamoto (2013)
The Role of Nanoparticle PEGylation in Drug Delivery
Ellina A. Mun (2020)
Design of magnetic gene complexes as effective and serum resistant gene delivery systems for mesenchymal stem cells.
T. Zhang (2017)
Scalable fractionation of iron oxide nanoparticles using a CO2 gas-expanded liquid system
Pranav S. Vengsarkar (2015)
The effect of surface charge of functionalized Fe3O4 nanoparticles on protein adsorption and cell uptake.
M. P. Calatayud (2014)
Cancer stem cell labeling using poly(L-lysine)-modified iron oxide nanoparticles.
Xueqin Wang (2012)
Atherosclerotic imaging using 4 types of superparamagnetic iron oxides: new possibilities for mannan-coated particles.
K. Tsuchiya (2013)
Iodoacetyl-functionalized pullulan: A supplemental enhancer for single-domain antibody-polyclonal antibody sandwich enzyme-linked immunosorbent assay for detection of survivin.
T. Matsushita (2017)
Preparation of Biodegradable Gelatin Nanospheres with a Narrow Size Distribution for Carrier of Cellular Internalization of Plasmid DNA
Norio Doi (2012)
Mesenchymal stem cells: a potential targeted-delivery vehicle for anti-cancer drug, loaded nanoparticles.
Zibin Gao (2013)
Effect of surface charge on the cellular uptake of fluorescent magnetic nanoparticles
S. Kralj (2012)
Biological effects of iron oxide‐protamine sulfate complex on mesenchymal stem cells and its relaxometry based labeling optimization for cellular MRI
S. Mishra (2017)
Modelling the mesenchymal stem cell niche in vitro using magnetic nanoparticles
Emily Elizabeth Louise Lewis (2016)
Magnetic Field-Responsive Nanocarriers
A. Jhaveri (2016)
See more
Semantic Scholar Logo Some data provided by SemanticScholar