Online citations, reference lists, and bibliographies.
← Back to Search

Aptamer-navigated Copolymeric Drug Carrier System For In Vitro Delivery Of MgO Nanoparticles As Insulin Resistance Reversal Drug Candidate In Type 2 Diabetes

Kei Xian Tan, J. Jeevanandam, S. Pan, L. S. Yon, M. Danquah
Published 2020 · Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
Abstract Magnesium Oxide (MgO) nanoparticles are known to play a significant role in lowering the production of lipids and serum glucose for diabetes treatment. Additionally, the emergence of aptamers has created new opportunities in the realization of smart drug delivery systems, mainly attributing to their abilities to be altered at the molecular level, and subsequently be conjugated onto biopolymers harbouring specific drugs, in order to navigate specific cellular sites. The current work reports the synthesis of a multifunctional aptamer-navigated particulate delivery system (DPAP), harbouring MgO (synthesized via chemical - MgO1 and green – MgO2 approaches) nanoparticles, to target 3T3-L1 diabetic cells. In vitro performance indicators, including encapsulation efficiency, targeting capability, cellular transfection efficiency, and insulin reversal capacity, were investigated. While the DLS analysis indicated hydrodynamic sizes of 766.6 nm and 641.3 nm for the DPAP-MgO1 and DPAP-MgO2 formulations, respectively; and zeta potentials of +5.28 mV and −0.025 mV, for the same formulations, respectively. Additionally, DPAP-MgO2 offered better encapsulation efficiency and loading capacity of 93.69% and 0.03 mg MgO/mg PLGA, respectively. 3,5-Dinitrosalicylic acid (DNS) assessment showed that both DPAP-MgO1 and DPAP-MgO2 particulate systems enhanced the in vitro cellular uptake, as well as the insulin resistance reversal ability of the 3T3-L1 cells. The outcomes from the current work demonstrate the potential of DPAP particulate system as an effective and promising carrier, for the delivery of MgO nanoparticles in diabetes treatment.
This paper references
10.1016/j.ijpharm.2014.12.035
Epithelial cell adhesion molecule aptamer conjugated PEG-PLGA nanopolymersomes for targeted delivery of doxorubicin to human breast adenocarcinoma cell line in vitro.
Mona Alibolandi (2015)
10.1186/s12929-014-0108-9
EpCAM aptamer mediated cancer cell specific delivery of EpCAM siRNA using polymeric nanocomplex
N. Subramanian (2015)
10.1038/mt.2014.51
Aptamer-targeted Antigen Delivery
B. Wengerter (2014)
10.3390/ijms17071040
Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review
F. J. Ruiz-Ojeda (2016)
10.1016/j.biomaterials.2011.01.021
The effect of surface charge on in vivo biodistribution of PEG-oligocholic acid based micellar nanoparticles.
K. Xiao (2011)
10.2174/0929867325666181008142831
Aptamer Based Targeted Drug Delivery Systems: Current Potential and Challenges.
Fen He (2018)
Enhanced Synthesis and Delivery of Magnesium Oxide Nanoparticles for Reverse Insulin Resistance in Type 2 Diabetes Mellitus
J. Jeevanandam (2017)
10.1007/s12010-019-03166-z
Cytotoxicity Analysis of Morphologically Different Sol-Gel-Synthesized MgO Nanoparticles and Their In Vitro Insulin Resistance Reversal Ability in Adipose cells
J. Jeevanandam (2019)
10.3390/I8020081
Efficient Gene Transfection into Mammalian Cells Mediated by Cross-linked Polyethylenimine
Wei Dong (2007)
10.1002/SLCT.201701911
Calcination‐Dependent Morphology Transformation of Sol‐Gel‐ Synthesized MgO Nanoparticles
J. Jeevanandam (2017)
10.1039/C6NJ03176E
Biosynthesis and characterization of MgO nanoparticles from plant extracts via induced molecular nucleation
J. Jeevanandam (2017)
10.1080/24701556.2019.1569053
Plant mediated green synthesis and nanoencapsulation of MgO nanoparticle from Calotropis gigantea: Characterisation and kinetic release studies
Yiik Siang Hii (2018)
10.1038/mtna.2014.32
Oligonucleotide Aptamers: New Tools for Targeted Cancer Therapy
Hongguang Sun (2014)
10.1016/J.POWTEC.2016.01.042
Carrier-based dry powder formulation for nasal delivery of vaccines utilizing BSA as model drug
Sabrina Trows (2016)
10.1021/acsomega.7b01134
Dihydrosanguinarine Enhances Glucose Uptake in Mouse 3T3-L1 Cells
Yit-Lai Chow (2017)
10.1016/J.ARCMED.2005.01.004
Complementary therapies for diabetes: the case for chromium, magnesium, and antioxidants.
F. Guerrero-Romero (2005)
10.1371/journal.pone.0060989
Effects of Microparticle Size and Fc Density on Macrophage Phagocytosis
Patricia Marie Pacheco (2013)
10.1007/s10867-008-9094-z
Initial Development and Characterization of PLGA Nanospheres Containing Ropivacaine
C. M. Moraes (2007)
10.3390/md14050090
Effect of Experimental Parameters on Alginate/Chitosan Microparticles for BCG Encapsulation
Liliana Aranha Caetano (2016)
10.4103/1735-1995.172811
Biochemical and molecular evidences on the protection by magnesium oxide nanoparticles of chlorpyrifos-induced apoptosis in human lymphocytes
Vida Heydary (2015)
10.1016/j.ijpharm.2012.06.036
In vitro controlled release of antihypertensive drugs intercalated into unmodified SBA-15 and MgO modified SBA-15 matrices.
Iuliana Florentina Alexa (2012)
10.1016/j.ejps.2012.10.014
Therapeutic RNA aptamers in clinical trials.
P. Sundaram (2013)
10.1038/nbt.3330
Principles of nanoparticle design for overcoming biological barriers to drug delivery
E. Blanco (2015)
10.1016/j.biopha.2018.03.052
Process evaluation and in vitro selectivity analysis of aptamer-drug polymeric formulation for targeted pharmaceutical delivery.
Kei Xian Tan (2018)
10.1166/JNN.2011.3551
In vitro anti-bacterial and cytotoxic properties of silver-containing poly(L-lactide-co-glycolide) nanofibrous scaffolds.
Zhi-Cai Xing (2011)
10.5958/0973-9130.2019.00381.5
Abundant Active Ingredients of Green Tea Regulate Glucose Metabolism in 3T3-L1 Cell Line
Sattar J. J. Al-shaeli (2019)
10.1016/J.AJPS.2015.09.004
Effect of process and formulation variables on the preparation of parenteral paclitaxel-loaded biodegradable polymeric nanoparticles: A co-surfactant study
N. Sharma (2016)
10.1007/s12011-016-0754-8
Functional Improvement in Rats’ Pancreatic Islets Using Magnesium Oxide Nanoparticles Through Antiapoptotic and Antioxidant Pathways
Shermineh Moeini-Nodeh (2016)
10.3390/ijms19061547
Dual Effects of Metformin on Adipogenic Differentiation of 3T3-L1 Preadipocyte in AMPK-Dependent and Independent Manners
D. Chen (2018)
10.1016/j.addr.2018.09.011
Aptamer‐guided nanomedicines for anticancer drug delivery
W. Alshaer (2018)
10.2147/DDDT.S99651
Preparation, characterization, and potential application of chitosan, chitosan derivatives, and chitosan metal nanoparticles in pharmaceutical drug delivery
T. Ahmed (2016)
10.22034/IJPS.2014.15248
Effect of nano-magnesium oxide on glucose concentration and lipid profile in diabetic laboratory mice
N. Naghsh (2014)
10.1016/j.molcel.2016.11.015
PARP-1 Controls the Adipogenic Transcriptional Program by PARylating C/EBPβ and Modulating Its Transcriptional Activity.
Xin Luo (2017)
10.1016/J.PATHOPHYS.2006.09.004
Increased magnesium intake prevents hyperlipidemia and insulin resistance and reduces lipid peroxidation in fructose-fed rats.
L. Olatunji (2007)
10.3390/ijms13033341
Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine
Mao Ye (2012)
10.7508/NMJ.2015.05.006
INTERACTION BETWEEN ANXIOLYTIC EFFECTS OF MAGNESIUM OXIDE NANOPARTICLES AND EXERCISE IN ADULT MALE RAT
M. Kesmati (2014)
10.1002/btpr.2524
Biophysical characterization of layer‐by‐layer synthesis of aptamer‐drug microparticles for enhanced cell targeting
Kei Xian Tan (2018)
10.1111/fcp.12387
Effect of magnesium sulfate administration to improve insulin resistance in type 2 diabetes animal model: using the hyperinsulinemic‐euglycemic clamp technique
S. Sohrabipour (2018)
10.1016/j.ab.2012.03.005
Protocol for effective differentiation of 3T3-L1 cells to adipocytes.
K. Zebisch (2012)
10.1186/s12906-015-0524-8
Exploring the anti-diabetic potential of Australian Aboriginal and Indian Ayurvedic plant extracts using cell-based assays
V. Gulati (2015)
10.1039/C6TB00651E
Dual cellular stimuli-responsive hydrogel nanocapsules for delivery of anticancer drugs.
Qiangying Yi (2016)
10.1016/J.CARBON.2015.12.027
Toxicity of carbon dots – Effect of surface functionalization on the cell viability, reactive oxygen species generation and cell cycle
M. Havrdová (2016)
10.1007/s12645-011-0024-6
Aptamer-labeled PLGA nanoparticles for targeting cancer cells
A. Aravind (2012)
10.1016/j.biomaterials.2008.03.011
Enhancement of poly(orthoester) microspheres for DNA vaccine delivery by blending with poly(ethylenimine).
David N. Nguyen (2008)
10.1038/s41392-017-0004-3
Controlled drug delivery vehicles for cancer treatment and their performance
S. Senapati (2018)
10.1016/j.ejps.2016.08.061
Towards targeted cancer therapy: Aptamer or oncolytic virus?
Kei Xian Tan (2017)
10.1016/j.jsps.2014.10.001
Microparticles as controlled drug delivery carrier for the treatment of ulcerative colitis: A brief review
Nidhi (2016)
10.1038/s41598-019-49458-6
Effect of Cucumis sativus on Dysfunctional 3T3-L1 Adipocytes
Méndez-Martínez Marisol (2019)
10.1016/j.xphs.2019.03.037
Binding Characterization of Aptamer-Drug Layered Microformulations and In Vitro Release Assessment.
Kei Xian Tan (2019)
10.1016/j.biomaterials.2011.07.004
Aptamer-functionalized PEG-PLGA nanoparticles for enhanced anti-glioma drug delivery.
Jianwei Guo (2011)
10.1155/2014/385135
Preparation and Characterization of Novel PBAE/PLGA Polymer Blend Microparticles for DNA Vaccine Delivery
Meenashi Vanathi Balashanmugam (2014)
Evaluation of Anticonvulsive Effect of Magnesium Oxide Nanoparticles in Comparison with Conventional MgO in Diabetic and Non-diabetic Male Mice
L. Jahangiri (2014)
10.1007/s12079-012-0188-9
Novel method to differentiate 3T3 L1 cells in vitro to produce highly sensitive adipocytes for a GLUT4 mediated glucose uptake using fluorescent glucose analog
Divya Vishwanath (2012)
10.2337/diacare.21.5.682
The Effect of Magnesium Supplementation in Increasing Doses on the Control of Type 2 Diabetes
Maria de Lourdes Lima (1998)
10.2217/nnm-2018-0180
AS1411 aptamer-targeted gold nanoclusters effect on the enhancement of radiation therapy efficacy in breast tumor-bearing mice.
Fatemeh Ghahremani (2018)
10.1007/s11626-997-0025-2
3T3-L1 adipocytes as a cell culture model of insulin resistance
V. P. Knutson (1997)
10.1016/j.biomaterials.2010.11.035
Dual-aptamer-based delivery vehicle of doxorubicin to both PSMA (+) and PSMA (-) prostate cancers.
Kyoungin Min (2011)
10.1002/bit.24558
AS1411 aptamer tagged PLGA‐lecithin‐PEG nanoparticles for tumor cell targeting and drug delivery
A. Aravind (2012)



Semantic Scholar Logo Some data provided by SemanticScholar