Online citations, reference lists, and bibliographies.

Simple Method For Determination Of Triazolam In Human Plasma By High-performance Liquid Chromatography/tandem Mass Spectrometry.

Xiao-Pen Lee, Takeshi Kumazawa, Masaya Fujishiro, Chika Hasegawa, Akemi Marumo, Yukiko Shoji, Tetsuya Arinobu, Hiroshi Seno, Keizo Sato
Published 2006 · Chemistry, Medicine
Cite This
Download PDF
Analyze on Scholarcy
Triazolam was analyzed from human plasma samples by high-performance liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) with an MSpak GF polymer column (50 mm x 4.6 mm i.d., particle size 6 microm), which enabled direct injection of crude biological samples. Separation of triazolam, and lorazepam as the internal standard (IS) was carried out using 10mM ammonium acetate (pH 3.56)-0.1% formic acid and an acetonitrile gradient elution. Both compounds formed base peaks due to [M + H]+ ions by HPLC/ESI-MS, and product ions were produced from each [M + H]+ ion as seen by HPLC-MS/MS. Quantification of triazolam and the IS in plasma samples was made by selective reaction monitoring using each base peak of product ions of HPLC-MS/MS. The recovery range of triazolam spiked into plasma was 86.4-92.7%. The regression equation for triazolam showed excellent linearity in the range of 0.25-20 ng/mL, and the detection limit was 0.1 ng/mL. Intra- and inter-day precisions for triazolam in plasma samples were not greater than 12.4%. Accuracy for the drug was in the range of 88.0-101.4%. Data obtained after oral administration of triazolam in male and female subjects are also presented.

This paper is referenced by
Determination of nonsteroidal anti-inflammatory drugs in human plasma by LC-MS-MS with a hydrophilic polymer column
Xiao-Pen Lee (2010)
Characterisation of oxazepam degradation products by high-performance liquid chromatography/electrospray ionisation mass spectrometry and electrospray ionisation quadrupole time-of-flight tandem mass spectrometry.
Thomas J P Smyth (2010)
Identification and Determination of Synthetic Pharmaceuticals as Adulterants in Eight Common Herbal Weight Loss Supplements
Marjan Khazan (2014)
Analytical methodologies for the determination of benzodiazepines in biological samples.
Karolina Persona (2015)
Analyses of benzodiazepines and their metabolites in various biological matrices by LC-MS(/MS).
Mitsuhiro Nakamura (2011)
Analytical methods for determination of benzodiazepines. A short review
Paulina Szatkowska (2014)
Rapid and highly sensitive analysis of benzodiazepines and tandospirone in human plasma by automated on-line column-switching UFLC-MS/MS.
Xiao-Pen Lee (2017)
LC-MS determination of triazolam and its hydroxy metabolites in mouse dried blood spots: application to transgenic mouse pharmacokinetic studies.
Prashant Kole (2017)
Benzodiazepines: sample preparation and HPLC methods for their determination in biological samples.
V. Samanidou (2009)
Quantitation of benzodiazepines in whole blood by electron impact-gas chromatography-mass spectrometry.
Nicholas B Tiscione (2008)
Imprinted functionalized silica sol-gel for solid-phase extraction of triazolamin.
Guoyou Jin (2011)
On-site rapid screening of benzodiazepines in dietary supplements using pipette-tip micro-solid phase extraction coupled to ion mobility spectrometry.
Tangqiang Sun (2019)
LC-MS in analytical toxicology: some practical considerations.
Lewis Couchman (2011)
Semantic Scholar Logo Some data provided by SemanticScholar