Online citations, reference lists, and bibliographies.
← Back to Search

PH Effects On The Hyaluronan Hydrolysis Catalysed By Hyaluronidase In The Presence Of Proteins: Part I. Dual Aspect Of The PH-dependence.

H. Lenormand, B. Deschrevel, J. Vincent
Published 2010 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Hyaluronan (HA) hydrolysis catalysed by hyaluronidase (HAase) is strongly inhibited when performed at a low ratio of HAase to HA concentrations and at low ionic strength. This is because long HA chains can form non-active complexes with HAase. Bovine serum albumin (BSA) is able to compete with HAase to form electrostatic complexes with HA so freeing HAase which then recovers its catalytic activity. This BSA-dependence is characterised by two main domains separated by the optimal BSA concentration: below this concentration the HAase activity increases when the BSA concentration is increased, above this concentration the HAase activity decreases. This occurs provided that HA is negatively charged and BSA is positively charged, i.e. in a pH range from 3 to 5.25. The higher the pH value the higher the optimal BSA concentration. Other proteins can also modulate HAase activity. Lysozyme, which has a pI higher than that of BSA, is also able to compete with HAase to form electrostatic complexes with HA and liberate HAase. This occurs over a wider pH range that extends from 3 to 9. These results mean that HAase can form complexes with HA and recover its enzymatic activity at pH as high as 9, consistent with HAase having either a high pI value or positively charged patches on its surface at high pH. Finally, the pH-dependence of HAase activity, which results from the influence of pH on both the intrinsic HAase activity and the formation of complexes between HAase and HA, shows a maximum at pH 4 and a significant activity up to pH 9.
This paper references
10.1016/S0945-053X(01)00172-X
The six hyaluronidase-like genes in the human and mouse genomes.
A. B. Csóka (2001)
10.1006/ABIO.2001.5068
An improved assay for the N-acetyl-D-glucosamine reducing ends of polysaccharides in the presence of proteins.
T. Astériou (2001)
10.1016/0003-2697(81)90159-7
A recommended procedure for the estimation of bovine testicular hyaluronidase in the presence of human serum.
P. Gacesa (1981)
10.1016/S0014-5793(98)00523-7
TSG‐6 interacts with hyaluronan and aggrecan in a pH‐dependent manner via a common functional element: implications for its regulation in inflamed cartilage
A. A. Parkar (1998)
10.1016/s0021-9258(19)52721-1
A turbidimetric method for the assay of hyaluronidase.
A. Dorfman (1948)
Hyaluronidases in tissue invasion.
T. Csóka (1997)
10.1016/S0959-8049(05)80214-X
Hyaluronan and hyaluronectin in the extracellular matrix of human brain tumour stroma.
B. Delpech (1993)
10.1016/S0927-7765(97)00005-2
AFM investigation of the adsorption process of bovine serum albumin on mica
O. Mori (1997)
10.3109/03008207509152337
Effect of ionic strength and pH on the properties of purified bovine testicular hyaluronidase.
S. Gorham (1975)
10.1016/S0308-8146(98)00238-6
Characterisation of Norway lobster (Nephrops norvegicus) hyaluronidase and comparison with sheep and bovine testicular hyaluronidase
Ashok M. Krishnapillai (1999)
10.1016/S0945-053X(01)00185-8
Inhibitors of the hyaluronidases.
K. Mio (2002)
10.1016/0022-1759(87)90508-4
An indirect enzymoimmunological assay for hyaluronidase.
B. Delpech (1987)
Tumor-associated hyaluronic acid: a new sensitive and specific urine marker for bladder cancer.
V. Lokeshwar (1997)
10.4024/40702.JBPC.07.04
How electrostatic interactions can change the kinetic behaviour of a Michaelis-Menten type enzyme. Application to the hyaluronan/hyaluronidase system
H. Lenormand (2007)
10.1002/(SICI)1097-0215(19970410)71:2<251::AID-IJC21>3.0.CO;2-J
Early‐response gene signalling is induced by angiogenic oligosaccharides of hyaluronan in endothelial cells. Inhibition by non‐angiogenic, high‐molecular‐weight hyaluronan
R. Deed (1997)
10.1016/s0021-9258(18)73382-6
Mucinase: a Bacterial Enzyme which Hydrolyzes Synovial Fluid Mucin and other Mucins.
W. B. Robertson (1940)
10.1126/SCIENCE.2408340
Angiogenesis induced by degradation products of hyaluronic acid.
D. West (1985)
The biology of hyaluronan.
D. Evered (1989)
10.1016/J.COCIS.2005.05.007
Polyelectrolyte-protein complexes
Christy L. Cooper (2005)
10.1021/bp960013+
Protein Separation via Polyelectrolyte Coacervation: Selectivity and Efficiency
Y. Wang (1996)
10.1016/j.semcancer.2008.03.017
Hyaluronidases in cancer biology.
R. Stern (2008)
Effect of protein charge heterogeneity in protein–polyelectrolyte
J. M. Park (1992)
10.1016/S0927-7765(97)00025-8
Determining isoelectric points of model proteins and Bacillus subtilis neutral protease by the cross partitioning using poly(ethylene glycol)/Dextran aqueous two-phase systems
J. H. Han (1997)
10.1006/ABIO.1998.3025
Activation and inhibition of human cancer cell hyaluronidase by proteins.
C. Maingonnat (1999)
10.1021/BM005656Z
Identification by integrated computer modeling and light scattering studies of an electrostatic serum albumin-hyaluronic acid binding site.
K. Grymonpré (2001)
10.1248/CPB.48.779
Characteristics of complexes composed of sodium hyaluronate and bovine serum albumin.
S. Xu (2000)
10.1016/S0006-3495(01)75847-X
Analytic binding isotherms describing competitive interactions of a protein ligand with specific and nonspecific sites on the same DNA oligomer.
O. V. Tsodikov (2001)
Hyaluronan. Woodhead Publishing, Wrexham, Wales
J. F. Kennedy (2002)
10.1016/0003-2697(62)90095-7
A modified uronic acid carbazole reaction.
T. Bitter (1962)
10.1080/10408699891274354
Structure and technofunctional properties of protein-polysaccharide complexes: a review.
C. Schmitt (1998)
10.3109/00016488709102833
Biochemistry of hyaluronan.
T. Laurent (1987)
10.1006/ABBI.1997.0365
Dependence of salt concentration on glycosaminoglycan-lysozyme interactions in cartilage.
J. M. Moss (1997)
10.1016/s0021-9258(17)35155-4
A new specific color reaction of hexuronic acids.
Z. Dische (1947)
10.1002/bip.21061
Electrostatic interactions between hyaluronan and proteins at pH 4: how do they modulate hyaluronidase activity.
H. Lenormand (2008)
10.1002/IJC.2910600511
The role of hyaluronan in tumour neovascularization (review)
P. Rooney (1995)
10.7326/0003-4819-65-4-875_4
Hyaluronidase and cancer
E. Cameron (1966)
Hyaluronidases in cancer
R. Stern (2008)
Hyaluronic acid, cell adhesion and metastasis
Jonathan B Catterall (1995)
10.1006/BBRC.1997.6773
Purification, cloning, and expression of human plasma hyaluronidase.
G. Frost (1997)
10.1016/s0021-9258(18)65959-9
A modified colorimetric method for the estimation of N-acetylamino sugars.
J. L. Reissig (1955)
10.1016/0003-9861(67)90037-9
A comparison of serum and testicular hyaluronidase.
M. De Salegui (1967)
10.1016/0003-9861(85)90839-2
Light-scattering study on the influence of link-glycoproteins and lysozyme on the hyaluronate molecular conformation in solution.
L. N. Blanco (1985)
10.1002/BIP.1980.360190713
An interaction of albumin with hyaluronic acid and chondroitin sulfate: A study of affinity chromatography and circular dichroism
E. W. Gold (1980)
10.1016/j.matbio.2009.04.008
The hyaluronan-protein complexes at low ionic strength: how the hyaluronidase activity is controlled by the bovine serum albumin.
H. Lenormand (2009)
10.1016/J.CARBPOL.2006.04.021
Interactions between furcellaran and the globular proteins bovine serum albumin and β-lactoglobulin
K. Laos (2007)
10.1016/B978-012374178-3.10011-0
CHAPTER 11 – Hyaluronidase: Both a Tumor Promoter and Suppressor
V. B. Lokeshwar (2009)
10.1016/0079-6700(92)90004-I
Functional immobilized biocatalysts
E. Kokufuta (1992)
10.1016/j.bpc.2009.09.010
How hyaluronan-protein complexes modulate the hyaluronidase activity: the model.
J. Vincent (2009)
10.1016/J.MATBIO.2005.11.005
Inhibition of hyaluronan hydrolysis catalysed by hyaluronidase at high substrate concentration and low ionic strength.
T. Astériou (2006)
10.1021/JP980486U
Complex Formation between Bovine Serum Albumin and Strong Polyelectrolytes: Effect of Polymer Charge Density
K. Mattison (1998)
10.4024/2030301.JBPC.03.02
Kinetics of hyaluronan hydrolysis catalysed by hyaluronidase
J. Vincent (2002)
10.1002/9780470513774.CH12
Hyaluronan and angiogenesis.
D. West (1989)
10.1021/BM025664A
Ionic strength dependence of protein-polyelectrolyte interactions.
Emek Seyrek (2003)
10.1016/J.MATBIO.2007.11.002
Hyaluronidase activity is modulated by complexing with various polyelectrolytes including hyaluronan.
B. Deschrevel (2008)
Functional properties of protein-polysaccharide mixtures
V. Tolstoguzov (1985)
10.1021/MA00027A047
Effects of protein charge heterogeneity in protein-polyelectrolyte complexation
J. Park (1992)
10.1006/ABBI.1994.1134
Binding properties of glycosaminoglycans to lysozyme--effect of salt and molecular weight.
M. van Damme (1994)
10.1046/j.1365-2796.1997.00172.x
Hyaluronan: fundamental principles and applications in cancer
B. Delpech (1997)
10.1016/J.LFS.2007.02.037
The magic glue hyaluronan and its eraser hyaluronidase: a biological overview.
K. Girish (2007)
10.1080/03602549408001288
Protein Purification by Selective Phase Separation with Polyelectrolytes
P. Dubin (1994)



This paper is referenced by
10.1016/J.CARBPOL.2011.06.044
pH effects on the hyaluronan hydrolysis catalysed by hyaluronidase in the presence of proteins. Part III. The electrostatic non-specific hyaluronan–hyaluronidase complex
H. Lenormand (2011)
Sélection et mise en oeuvre "optimale" de souches microbiennes en bioréacteur, pour la production d'acide hyaluronique
Pierrick Leblanc (2014)
10.1016/j.ab.2010.04.014
A complete hyaluronan hydrodynamic characterization using a size exclusion chromatography-triple detector array system during in vitro enzymatic degradation.
A. La Gatta (2010)
10.1016/j.bpc.2013.02.007
Reaction-complexation coupling between an enzyme and its polyelectrolytic substrate: determination of the dissociation constant of the hyaluronidase-hyaluronan complex from the hyaluronidase substrate-dependence.
H. Lenormand (2013)
10.1016/J.CARBPOL.2010.06.011
Chain length effects on electrostatic interactions between hyaluronan fragments and albumin
H. Lenormand (2010)
10.1016/j.ejps.2014.07.011
Determination of the presence of hyaluronic acid in preparations containing amino acids: the molecular weight characterization.
A. Bellomaria (2014)
10.1016/j.ab.2015.04.008
Kinetic investigation of recombinant human hyaluronidase PH20 on hyaluronic acid.
Shiping Fang (2015)
10.3390/polym11071097
Protein–Polyelectrolyte Complexes and Micellar Assemblies
Shang Gao (2019)
10.3390/polym12092069
Hyaluronan-Arginine Interactions—An Ultrasound and ITC Study
Adam Jugl (2020)
10.1016/J.CARBPOL.2011.02.007
pH effects on the hyaluronan hydrolysis catalysed by hyaluronidase in the presence of proteins: Part II. The electrostatic hyaluronan – Protein complexes
H. Lenormand (2011)
Semantic Scholar Logo Some data provided by SemanticScholar