Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

In Vitro And In Vivo Evaluation Of Chitosan-gelatin Scaffolds For Cartilage Tissue Engineering.

Shu Wen Whu, Kun-Che Hung, K. Hsieh, C. Chen, C. Tsai, S. Hsu
Published 2013 · Materials Science, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Chitosan-gelatin polyelectrolyte complexes were fabricated and evaluated as tissue engineering scaffolds for cartilage regeneration in vitro and in vivo. The crosslinker for the gelatin component was selected among glutaraldehyde, bisepoxy, and a water-soluble carbodiimide (WSC) based upon the proliferation of chondrocytes on the crosslinked gelatin. WSC was found to be the most suitable crosslinker. Complex scaffolds made from chitosan and gelatin with a component ratio equal to one possessed the proper degradation rate and mechanical stability in vitro. Chondrocytes were able to proliferate well and secrete abundant extracellular matrix in the chitosan-gelatin (1:1) complex scaffolds crosslinked by WSC (C1G1WSC) compared to the non-crosslinked scaffolds. Implantation of chondrocytes-seeded scaffolds in the defects of rabbit articular cartilage confirmed that C1G1WSC promoted the cartilage regeneration. The neotissue formed the histological feature of tide line and lacunae in 6.5 months. The amount of glycosaminoglycans in C1G1WSC constructs (0.187±0.095 μg/mg tissue) harvested from the animals after 6.5 months was 14 wt.% of that in normal cartilage (1.329±0.660 μg/mg tissue). The average compressive modulus of regenerated tissue at 6.5 months was about 0.539 MPa, which approached to that of normal cartilage (0.735 MPa), while that in the blank control (3.881 MPa) was much higher and typical for fibrous tissue. Type II collagen expression in C1G1WSC constructs was similarly intense as that in the normal hyaline cartilage. According to the above results, the use of C1G1WSC scaffolds may enhance the cartilage regeneration in vitro and in vivo.
This paper references
10.1002/PEN.760272209
Can the gel point of a cross-linking polymer be detected by the G′ – G″ crossover?
H. Winter (1987)
Carbohydr
H. Nagahama (2009)
Biol
S. W. Whu (2009)
10.1016/J.JTICE.2009.08.011
Effect of bovine pituitary extract on the formation of neocartilage in chitosan/gelatin scaffolds
Y. Kuo (2010)
A
B. Grigolo (2001)
Anal
Y. J. Kim (1988)
T
T. Matsumoto (1993)
10.1201/b18423
The Biomedical Engineering Handbook
J. Bronzino (1995)
Drug Dev
N. H. Foda (2007)
10.1016/J.POLYMDEGRADSTAB.2008.10.017
Characterization and biodegradation of chitosan-alginate polyelectrolyte complexes
X. Li (2009)
10.1016/J.BIOMATERIALS.2003.09.066
Influence of different collagen species on physico-chemical properties of crosslinked collagen matrices.
P. Angele (2004)
10.1088/1748-6041/1/3/010
The effect of a chitosan-gelatin matrix and dexamethasone on the behavior of rabbit mesenchymal stem cells.
G. Medrado (2006)
10.1016/S0142-9612(00)00429-4
Transplantation of chondrocytes seeded on a hyaluronan derivative (hyaff-11) into cartilage defects in rabbits.
B. Grigolo (2001)
Eur
T. Delair (2011)
10.1016/0032-3861(93)90596-3
Viscoelastic behaviour at the thermal sol-gel transition of gelatin
S. Hsu (1993)
Y
K. Tomihata (1993)
10.1016/S0169-409X(98)00023-4
Host response to tissue engineered devices.
Mikos (1998)
10.1002/(SICI)1097-4636(20000315)49:4<534::AID-JBM12>3.0.CO;2-#
GAG-augmented polysaccharide hydrogel: a novel biocompatible and biodegradable material to support chondrogenesis.
V. Franklin Sechriest (2000)
Artif
S. Hsu (2002)
10.1007/8415_2010_51
Three-Dimensional Porous Scaffold of Hyaluronic Acid for Cartilage Tissue Engineering
Dae-Duk Kim (2010)
Stud
D. D. Kim (2011)
R
P. Angele (2004)
Adult Articular Cartilage
M. Freeman (1973)
10.1163/156856293X00131
A wettability gradient as a tool to study protein adsorption and cell adhesion on polymer surfaces.
J. Lee (1993)
Anal
B. O. Enobakhare (1996)
Sci
Y. F. Qian (2011)
10.1016/S0142-9612(99)00164-7
Retinal pigment epithelial cell function on substrates with chemically micropatterned surfaces.
L. Lu (1999)
10.2106/00004623-199704000-00021
Instructional Course Lectures, The American Academy of Orthopaedic Surgeons - Articular Cartilage. Part I: Tissue Design and Chondrocyte-Matrix Interactions*†
J. Buckwalter (1997)
10.1016/0003-2697(88)90532-5
Fluorometric assay of DNA in cartilage explants using Hoechst 33258.
Y. Kim (1988)
Tissue Eng
C. P. Barnes (2007)
10.1016/J.MSEC.2003.11.008
New type of biodegradable porous scaffolds for tissue-engineered articular cartilage
M. Kawanishi (2004)
10.1177/001452469000101110
"J."
G.G. Stokes (1890)
10.1016/j.ejpb.2010.12.001
Colloidal polyelectrolyte complexes of chitosan and dextran sulfate towards versatile nanocarriers of bioactive molecules.
T. Delair (2011)
L
H. Tan (2007)
10.1002/mrd.1080280417
Culture of Animal Cells: A Manual of Basic Technique
D. Tee (1984)
10.1006/ABIO.1996.0502
Quantification of sulfated glycosaminoglycans in chondrocyte/alginate cultures, by use of 1,9-dimethylmethylene blue.
B. Enobakhare (1996)
10.1080/03639040600975188
Implantable Biodegradable Sponges: Effect of Interpolymer Complex Formation of Chitosan With Gelatin on the Release Behavior of Tramadol Hydrochloride
N. Foda (2007)
Polym
H. Winter (1987)
Mater
W. Xia (2004)
10.1016/S0142-9612(02)00067-4
Crosslinked type II collagen matrices: preparation, characterization, and potential for cartilage engineering.
J. S. Pieper (2002)
Mater
M. Kawanishi (2004)
10.1016/S0142-9612(02)00442-8
Structure and properties of bilayer chitosan-gelatin scaffolds.
J. Mao (2003)
Sci
J. H. Lee (1993)
Arthritis Rheum
J. A. Buckwalter (1998)
10.1016/J.CARBPOL.2008.10.015
Preparation and characterization of novel chitosan/gelatin membranes using chitosan hydrogel
H. Nagahama (2009)
10.1111/J.1525-1594.2004.00046.X
Evaluation of chitosan-alginate-hyaluronate complexes modified by an RGD-containing protein as tissue-engineering scaffolds for cartilage regeneration.
S. Hsu (2004)
10.1163/092050610X499447
Cross-Linking of Gelatin and Chitosan Complex Nanofibers for Tissue-Engineering Scaffolds
Yongfang Qian (2011)
10.1016/J.MSEC.2006.03.014
Attachment of stem cells on porous chitosan scaffold crosslinked by Na5P3O10
H. Lin (2007)
Polym
X. Li (2009)
Bronzino (Ed.), The biomedical engineering handbook
L. E. Freed (2000)
10.1016/J.MSEC.2012.05.024
Synthesis and characterization of disulfide-crosslinked alginate hydrogel scaffolds
Y. Zhao (2012)
10.1007/S10856-007-3095-5
Gelatin/chitosan/hyaluronan ternary complex scaffold containing basic fibroblast growth factor for cartilage tissue engineering
H. Tan (2007)
Artif
S. Hsu (2004)
Chem
Y.-C. Kuo (2010)
10.1089/TEN.2006.0292
Cross-linking electrospun type II collagen tissue engineering scaffolds with carbodiimide in ethanol.
C. Barnes (2007)
10.1002/JBM.B.30087
Tissue engineering of cartilage with the use of chitosan-gelatin complex scaffolds.
W. Xia (2004)
10.1089/TEN.2006.12.3209
Repair of porcine articular osteochondral defects in non-weightbearing areas with autologous bone marrow stromal cells.
G. Zhou (2006)
Mater
Y. Zhao (2012)
Tissue Eng
J. Hodde (2002)
10.1046/J.1525-1594.2002.06889.X
Evaluation of cellular affinity and compatibility to biodegradable polyesters and Type-II collagen-modified scaffolds using immortalized rat chondrocytes.
S. Hsu (2002)
Philos
W. Hunter (1743)
10.1002/1529-0131(199808)41:8<1331::AID-ART2>3.0.CO;2-J
Articular cartilage repair and transplantation.
J. Buckwalter (1998)
10.1016/J.BIOMATERIALS.2004.02.067
Crosslinked hyaluronic acid hydrogels: a strategy to functionalize and pattern.
Tatiana Segura (2005)
Biomed
G.C.B. Medrado (2006)
Mater
V. F. Sechriest (2000)
Anal
M. Bergman (1996)
10.1016/S0142-9612(00)00126-5
Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review.
Jung Kee Suh (2000)
Tissue Eng
G. Zhou (2006)
10.1089/107632702753725058
Naturally occurring scaffolds for soft tissue repair and regeneration.
J. Hodde (2002)
10.3233/BIR-1993-305-614
Rheological properties and fractal structure of concentrated polyion complexes of chitosan and alginate.
T. Matsumoto (1993)
G
J. X. Lu (1999)
Mater
A. N. Jătariu Cadinoiu (2011)



This paper is referenced by
10.1080/09205063.2018.1433422
Design and fabrication of injectable microcarriers composed of acellular cartilage matrix and chitosan
Farzane Sivandzade (2018)
10.1252/JCEJ.16WE145
Toxic Chemical Cross-linker-free Cryosponges Made from Chitosan-Gluconic Acid Conjugate for Chondrocyte Culture
T. Takei (2017)
10.1088/1748-6041/11/6/065002
Development and fabrication of a two-layer tissue engineered osteochondral composite using hybrid hydrogel-cancellous bone scaffolds in a spinner flask.
Kedong Song (2016)
10.1016/j.msec.2015.05.062
Three-dimensional dynamic fabrication of engineered cartilage based on chitosan/gelatin hybrid hydrogel scaffold in a spinner flask with a special designed steel frame.
Kedong Song (2015)
10.1016/J.MSEC.2019.109927
The immune reaction and degradation fate of scaffold in cartilage/bone tissue engineering.
D. Yang (2019)
10.1007/978-981-13-8855-2_15
Clinical Application of Biomimetic Marine-Derived Materials for Tissue Engineering
V. Lalzawmliana (2019)
10.1016/j.ijbiomac.2016.11.010
Investigation of different cross-linking approaches on 3D gelatin scaffolds for tissue engineering application: A comparative analysis.
K. Shankar (2017)
10.1134/S1990519X19060087
Cellular Mechanisms of Bioresorption of a Porous 3D Matrix Based on Chitosan
P. Popryadukhin (2019)
10.3390/polym8040106
Interpenetration of Natural Polymer Aerogels by Supercritical Drying
L. Baldino (2016)
10.1186/s40824-016-0055-5
Polymer mesh scaffold combined with cell-derived ECM for osteogenesis of human mesenchymal stem cells
Yong Kwan Noh (2016)
10.1080/09205063.2014.987570
Effects of purified alginate sponge on the regeneration of chondrocytes: in vitro and in vivo
J. Song (2015)
10.1155/2017/8639898
Versatility of Chitosan-Based Biomaterials and Their Use as Scaffolds for Tissue Regeneration
J. C. V. Ribeiro (2017)
10.1039/C5RA27246G
3-Dimensional porous nanocomposite scaffolds based on cellulose nanofibers for cartilage tissue engineering: tailoring of porosity and mechanical performance†
Narges Naseri (2016)
10.1002/9781119143802.ch55
Recent Advances on Chitosan‐Based Materials in Regenerative Medicine
P. Sivashankari (2020)
10.3390/ma8115413
Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion
N. Mighri (2015)
10.3109/21691401.2015.1129624
Polyelectrolyte complexes: mechanisms, critical experimental aspects, and applications
A. Kulkarni (2016)
10.3390/membranes10110348
Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering
Monika Wasyłeczko (2020)
CELL GROWTH SCAFFOLDS OF CHITOSAN/GELATIN FOR USE IN TISSUE REGENERATION
A. I. Cañas (2014)
10.1007/978-81-322-2511-9_3
Chitosan-Based Scaffolds for Cartilage Regeneration
Xuezhou Li (2016)
10.1002/APP.40635
Evaluation of the chondrogenic differentiation of mesenchymal stem cells on hybrid biomimetic scaffolds
A. Karkhaneh (2014)
Evaluation of Biodegradability and Cell Functionality of Injectable Glycol Chitosan Hydrogel
A. Padmanabhan (2015)
10.1016/j.biotechadv.2014.04.014
Three-dimensional biomaterial degradation - Material choice, design and extrinsic factor considerations.
L. Yildirimer (2014)
Porous Scaffolds of Cellulose Nanofibres Bound with Crosslinked Chitosan and Gelatine for Cartilage Applications: : Processing and Characterisation
Jean-Michel Poirier (2013)
10.1016/B978-0-08-100230-8.00008-X
Production of micro- and nanoscale chitosan particles for biomedical applications
A. C. Jayasuriya (2017)
10.1155/2014/489128
Scaffolding biomaterials for cartilage regeneration
Z. Cao (2014)
10.1016/j.foodhyd.2020.106262
An integrated manufacturing strategy to fabricate delivery system using gelatin/alginate hybrid hydrogels: 3D printing and freeze-drying
Chih-Chun Kuo (2021)
10.2174/1872211308666141028145651
Injectable thermosensitive chitosan/glycerophosphate-based hydrogels for tissue engineering and drug delivery applications: a review.
F. G. Tahrir (2015)
10.2147/ORR.S63437
New biomaterials for orthopedic implants
Kevin L. Ong (2015)
10.1302/2046-3758.99.BJR-2019-0210.R2
In vivo cartilage regeneration in a multi-layered articular cartilage architecture mimicking scaffold
K. Rajagopal (2020)
10.1016/j.ijbiomac.2019.11.003
Natural Biomacromolecule based Composite Scaffolds from Silk Fibroin, Gelatin and Chitosan toward Tissue Engineering Applications.
Shiva Asadpour (2019)
10.1134/S1990519X18030094
Bioresorption of Porous 3D Matrices Based on Collagen in Liver and Muscular Tissue
P. Popryadukhin (2018)
10.1007/978-3-319-44785-8_5
Cartilage tissue engineering and regenerative strategies
A. S. Morais (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar