Online citations, reference lists, and bibliographies.

Acanthopanacis Cortex Extract: A Novel Photosensitizer For Head And Neck Squamous Cell Carcinoma Therapy.

Shuhan Shi, Hyejoung Cho, Qiaochu Sun, Yuzhu He, Guowu Ma, Young Ran Kim, Byung-gook Kim, Okjoon Kim
Published 2019 · Medicine
Cite This
Download PDF
Analyze on Scholarcy
Share
OBJECTIVES The aim of this study was to develop a novel photosensitizer from traditional plant extracts and to investigate the photodynamic therapy (PDT) effect and mechanism of action of the novel photosensitizer on KB and Hep-2 cells. METHODS Fluorescence emission, cell viability, and intracellular distribution of candidates were analyzed to screen potential photosensitizers from traditional plant extracts. Cellular reactive oxygen species (ROS) quantification, Annexin V-FITC/PI staining, and western blotting were performed to explore the mechanism of cell death in KB and Hep-2 cells. RESULT Of 289 traditional plant extracts, 13 plant extracts with strong fluorescence were initially screened by fluorescence emission analysis. The cell viability assay and intracellular distribution of candidates showed that Acanthopanacis Cortex (AC) extract is a potential photosensitizer. Under optimal PDT conditions, high levels of ROS were produced in KB and Hep-2 cells, followed by cell death. However, there was no significant damage to HaCaT cells. Moreover, apoptosis induced by AC extract with 625 nm irradiation (IR) down-regulated the expression of Bcl-2 protein and up-regulated the expression of Bax protein, as well as that of cleaved PARP-1 protein in both KB and Hep-2 cells. CONCLUSION The fluorescence intensity of AC extract at 420 nm is similar to that of the commercial Hematoporphyrin (HP). AC extract with 625 nm IR could enhance the PDT effect, induce ROS generation, and trigger apoptotic pathways in KB and Hep-2 cells. Therefore, we suggest that AC is a potential novel photosensitizer for PDT in head and neck squamous cell carcinoma.
This paper references
10.1016/S0040-4020(98)00015-5
Porphyrin-based Photosensitizers for Use in Photodynamic Therapy
Ethan Sternberg (1998)
10.1038/cdd.2009.219
Apoptosis in yeast: triggers, pathways, subroutines
Didac Carmona-Gutiérrez (2010)
10.3177/jnsv.49.327
A study of the antioxidative and antimutagenic effects of Houttuynia cordata Thunb. using an oxidized frying oil-fed model.
Ya Yen Chen (2003)
10.1080/03639045.2018.1496451
Comparative enhancement of curcumin cytotoxic photodynamic activity by nanoliposomes and gold nanoparticles with pharmacological appraisal in HepG2 cancer cells and Erlich solid tumor model
Maha Fadel (2018)
10.1016/j.biopha.2017.10.080
Inhibitory effect of aloe emodin mediated photodynamic therapy on human oral mucosa carcinoma in vitro and in vivo.
Yun-qing Liu (2018)
[Separation of eleutheroside E from crude extract of Radix Acanthopanacis Senticosus by analytical and preparative high-speed countercurrent chromatography].
Yun Wei (2002)
10.1615/CritRevEukarGeneExpr.v16.i4.10
Vascular and cellular targeting for photodynamic therapy.
Bin Chen (2006)
10.1016/S0076-6879(00)19037-8
Role of activated oxygen species in photodynamic therapy.
Wesley M. Sharman (2000)
10.3892/ol.2016.4179
Curative effect of the recent photofrin photodynamic adjuvant treatment on young patients with advanced colorectal cancer
Bo Sun (2016)
10.1097/MOO.0b013e328017f669
The economics of squamous cell carcinoma of the head and neck
Joseph Menzin (2007)
10.1007/BF02975842
A benzenoid from the stem ofAcanthopanax senticosus
Jiyoung Ryu (2004)
10.1016/j.pdpdt.2012.11.009
Monoglycoconjugated phthalocyanines: effect of sugar and linkage on photodynamic activity.
Dominique Lafont (2013)
10.1016/j.pdpdt.2015.12.009
Photodynamic therapy using Photofrin and excimer dye laser treatment for superficial oral squamous cell carcinomas with long-term follow up.
Shigeaki Toratani (2016)
10.1016/S1572-1000(04)00007-9
Photosensitizers in clinical PDT.
Ron R. Allison (2004)
[Understanding cell death: a challenge for biomedicine].
J VilchesTroya (2005)
10.1038/bjc.1992.402
Interstitial photodynamic therapy in a rat liver metastasis model.
Richard van Hillegersberg (1992)
10.1002/cmdc.201100186
Tissue uptake study and photodynamic therapy of melanoma-bearing mice with a nontoxic, effective chlorin.
Janusz M Dąbrowski (2011)
10.5978/ISLSM.16-OR-15
Mitochondrial Reactive Oxygen Species and Photodynamic Therapy.
Hiromu Ito (2016)
10.3390/molecules23020458
Trifluoromethyl Boron Dipyrromethene Derivatives as Potential Photosensitizers for Photodynamic Therapy
Jian-Yong Liu (2018)
10.3389/fneur.2018.00024
Intraoperative Photodynamic Diagnosis Using Talaporfin Sodium Simultaneously Applied for Photodynamic Therapy against Malignant Glioma: A Prospective Clinical Study
Kazuhide Shimizu (2018)
10.1021/np060195+
Biologically Active Triterpenoid Saponins from Acanthopanax senticosus
Wenhong Jiang (2006)
10.1016/j.ejmech.2011.07.057
Natural products: an evolving role in future drug discovery.
Bhuwan B. Mishra (2011)
10.1080/10286020903020578
Two new compounds from Acanthopanax senticosus Harms
Zhi-feng Li (2009)
10.1016/j.ultrasmedbio.2012.08.017
The Combined effects of hematoporphyrin monomethyl ether-SDT and doxorubicin on the proliferation of QBC939 cell lines.
Lei Liang (2013)
10.3892/etm.2017.5568
Anti-tumour effects of polysaccharide extracted from Acanthopanax senticosus and cell-mediated immunity.
Qinglong Meng (2018)
10.1021/acsomega.8b00692
Targeting of a Photosensitizer to the Mitochondrion Enhances the Potency of Photodynamic Therapy
Sakkarapalayam M. Mahalingam (2018)
10.1007/7651_2013_42
Growth and differentiation of HaCaT keratinocytes.
Van G. Wilson (2014)
[Experimental study on anti-tumor effects of cortex Acanthopanacis senticosus in vivo and in vitro].
Baoen Shan (2004)
10.14740/wjon1104w
Major Risk Factors in Head and Neck Cancer: A Retrospective Analysis of 12-Year Experiences
Anil Kumar Dhull (2018)
10.12998/wjcc.v1.i3.96
Monitoring photodynamic therapy of head and neck malignancies with optical spectroscopies.
Ulas Sunar (2013)
10.1039/c5pp00132c
Photodynamic therapy (PDT) of cancer: from local to systemic treatment.
Janusz M Dąbrowski (2015)
10.4236/CM.2013.43015
Houttuynia cordata Thunb: A Review of Phytochemistry and Pharmacology and Quality Control
Jiangang Fu (2013)
10.3760/cma.j.issn.1673-0860.2018.04.015
[Current status and prospect of photodynamic therapy in laryngeal diseases].
Cai-yun Zhang (2018)
10.18632/oncotarget.20189
Porphyrin photosensitizers in photodynamic therapy and its applications
Jiayuan Y Kou (2017)
10.1002/cncr.28905
Novel endoscopic diagnosis for bladder cancer.
Seth P. Lerner (2015)
10.1111/j.1751-1097.1997.tb08578.x
Lysosomes and microtubules as targets for photochemotherapy of cancer.
Kristian Berg (1997)
10.3390/cancers3022516
Cell Death Pathways in Photodynamic Therapy of Cancer
Paweł Mróz (2011)
Houttuynia cordata Thunb extract inhibits cell growth and induces apoptosis in human primary colorectal cancer cells.
Kuang-Chi Lai (2010)
10.3322/caac.20114
Photodynamic therapy of cancer: an update.
Patrizia Agostinis (2011)
10.3892/ol.2017.5915
The effect of aloe-emodin-induced photodynamic activity on the apoptosis of human gastric cancer cells: A pilot study
Hai-dan Lin (2017)
10.3390/molecules21111470
In Vitro Photodynamic Effect of Phycocyanin against Breast Cancer Cells
S Jagadeeswari Bharathiraja (2016)
10.1111/j.1756-5391.2010.01055.x
Improving reporting of adverse events and adverse drug reactions following injections of Chinese materia medica.
Zhaoxiang Bian (2010)
10.1248/BPB.33.1716
Isofraxidin, a coumarin component from Acanthopanax senticosus, inhibits matrix metalloproteinase-7 expression and cell invasion of human hepatoma cells.
Taisuke Yamazaki (2010)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar