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In Vitro And In Vivo Evaluation Of Chitosan-alginate/gentamicin Wound Dressing Nanofibrous With High Antibacterial Performance

H. R. Bakhsheshi-Rad, Z. Hadisi, A. F. Ismail, M. Aziz, M. Akbari, F. Berto, Xiongbiao Chen
Published 2020 · Materials Science

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Abstract Wound dressings based on nanofiber polymer scaffolds with good antimicrobial performance and skin reconstruction ability are promising options to thwart wound infection and accelerate wound healing. This paper reports on the synthesis via electrospinning of chitosan-alginate (CS-Alg) nanofiber dressings with various amounts of gentamicin (Gn; 0–10 wt%) as a drug delivery system. Smooth and continuous nanofibers with no obvious beads were created, with increases in the amount of Gn resulting in reduced fiber diameter. Antimicrobial tests showed the Gn-loaded nanofibers had good antibacterial performance as indicated by the inhibition of bacterial growth. CS-Alg nanofibers loaded with higher Gn concentrations exhibited greater antibacterial performance than those with lower Gn concentrations. In vitro cell culture studies demonstrated that CS-Alg wound dressings with 1–3% Gn improved L929 cell attachment and proliferation more than wound dressings with higher Gn concentrations. In vivo experiments revealed that Cs-Alg nanofibers loaded with 3% Gn significantly enhanced skin regeneration in a Balb/C mice model by stimulating the formation of a thicker dermis, increasing collagen deposition, and increasing the formation of new blood vessels and hair follicles. Collectively, Gn-loaded CS-Alg wound dressings can be considered a good candidate for drug delivery systems and skin regeneration applications.
This paper references
10.1016/J.POLYMERTESTING.2018.04.021
Characterization of scaffolds based on chitosan and collagen with glycosaminoglycans and sodium alginate addition
B. Kaczmarek (2018)
10.1016/J.POLYMERTESTING.2019.106039
Alginate membrane dressing toughened by chitosan floccule to load antibacterial drugs for wound healing
L. Xing (2019)
10.1016/j.msec.2016.09.031
Characterisation, in vitro release study, and antibacterial activity of montmorillonite-gentamicin complex material.
A. Rapacz-Kmita (2017)
10.3390/antibiotics8040234
Development of a Novel Pharmaceutical Formula of Nanoparticle Lipid Carriers of Gentamicin/α-Tocopherol and In Vivo Assessment of the Antioxidant Protective Effect of α-Tocopherol in Gentamicin-Induced Nephrotoxicity
M. Elfaky (2019)
10.1016/j.carbpol.2018.01.044
Microbicidal gentamicin-alginate hydrogels.
Stalin Kondaveeti (2018)
10.1016/J.POLYMERTESTING.2018.10.029
Fabrication of electrospun antibacterial PVA/Cs nanofibers loaded with CuNPs and AgNPs by an in-situ method
Davood Kharaghani (2018)
10.1016/j.ijbiomac.2017.07.144
Antimicrobial cerium ion-chitosan crosslinked alginate biopolymer films: A novel and potential wound dressing.
Hakan Kayğusuz (2017)
10.1007/s11356-015-4094-6
Reducing the bioavailability of cadmium in contaminated soil by dithiocarbamate chitosan as a new remediation
Zheng Yin (2015)
10.1016/J.POLYMERTESTING.2018.12.015
Fabrication of antibacterial electrospun cellulose acetate/ silver-sulfadiazine nanofibers composites for wound dressings applications
M. Q. Khan (2019)
10.2147/IJN.S65335
Biocomposite nanofibrous strategies for the controlled release of biomolecules for skin tissue regeneration
C. Gandhimathi (2014)
10.1021/nn400028p
Simultaneously strong and tough ultrafine continuous nanofibers.
Dimitry Papkov (2013)
10.1016/j.matlet.2019.126618
Antibacterial activity and in vivo wound healing evaluation of polycaprolactone-gelatin methacryloyl-cephalexin electrospun nanofibrous
H. R. Bakhsheshi-Rad (2019)
10.1016/j.carbpol.2017.06.026
Preparation and characterization of novel chitosan and β-cyclodextrin polymer sponges for wound dressing applications.
C. Flores (2017)
10.1016/J.CCLET.2018.01.023
Bioactive glass sol as a dual function additive for chitosan-alginate hybrid scaffold
Huihui Ren (2018)
10.1016/J.MATLET.2016.12.113
Ceramic scaffolds with immobilized vancomycin-loaded poly(lactide-co-glycolide) microparticles for bone defects treatment
Łucja Rumian (2017)
10.2147/IJN.S91975
Silver nanoparticles/chitosan oligosaccharide/poly(vinyl alcohol) nanofiber promotes wound healing by activating TGFβ1/Smad signaling pathway
Chen-wen Li (2016)
10.1038/srep06335
Micromechanics of ultra-toughened electrospun PMMA/PEO fibres as revealed by in-situ tensile testing in an electron microscope
Richard L Andersson (2014)
10.1166/JBN.2014.1748
Novel layer-by-layer structured nanofibrous mats coated by protein films for dermal regeneration.
Shangjing Xin (2014)
10.1016/j.msec.2015.11.057
Deposition of nanostructured fluorine-doped hydroxyapatite-polycaprolactone duplex coating to enhance the mechanical properties and corrosion resistance of Mg alloy for biomedical applications.
H. R. Bakhsheshi-Rad (2016)
10.1016/j.bioactmat.2017.11.003
Chitosan based metallic nanocomposite scaffolds as antimicrobial wound dressings
A. Mohandas (2018)
10.1016/J.SURFCOAT.2019.124898
Coating biodegradable magnesium alloys with electrospun poly-L-lactic acid-åkermanite-doxycycline nanofibers for enhanced biocompatibility, antibacterial activity, and corrosion resistance
H. R. Bakhsheshi-Rad (2019)
10.1016/J.CERAMINT.2019.03.071
Antibacterial activity and corrosion resistance of Ta2O5 thin film and electrospun PCL/MgO-Ag nanofiber coatings on biodegradable Mg alloy implants
H. R. Bakhsheshi-Rad (2019)
10.1016/J.MATLET.2017.07.072
Drug delivery and cytocompatibility of ciprofloxacin loaded gelatin nanofibers-coated Mg alloy
H. R. Bakhsheshi-Rad (2017)
10.3390/membranes1030249
Functionality in Electrospun Nanofibrous Membranes Based on Fiber's Size, Surface Area, and Molecular Orientation
H. Matsumoto (2011)
10.1177/0885328216653108
Hydroxyapatite microporous bioceramics as vancomycin reservoir: Antibacterial efficiency and biocompatibility investigation
M. Parent (2016)
10.1016/J.MATCHEMPHYS.2018.09.054
A new multifunctional monticellite-ciprofloxacin scaffold: Preparation, bioactivity, biocompatibility, and antibacterial properties
H. R. Bakhsheshi-Rad (2019)
10.1016/J.MATLET.2014.11.044
Polycaprolactone nanofibers for the controlled release of tetracycline hydrochloride
P. Karuppuswamy (2015)
10.1016/j.ijbiomac.2016.05.038
Curcumin loaded chitosan nanoparticles impregnated into collagen-alginate scaffolds for diabetic wound healing.
Veera Venkata Satyanarayana Reddy Karri (2016)
10.1016/J.POLYMERTESTING.2019.106022
Skin wound healing acceleration by Ag nanoparticles embedded in PVA/PVP/Pectin/Mafenide acetate composite nanofibers
R. Alipour (2019)
10.1080/00914030213038
Mechanical and Physical properties of chitosan and whey blended with poly(ε-caprolactone)
I. Olabarrieta (2002)
10.1016/j.carbpol.2015.06.050
Unusual effects of monocarboxylic acids on the structure and on the transport and mechanical properties of chitosan films.
F. Chen (2015)
10.1016/j.carbpol.2019.01.047
Layer-by-layer immobilization of amphoteric carboxymethyl chitosan onto biocompatible silk fibroin nanofibrous mats.
Hu Tu (2019)
10.1155/2018/9367034
Comparison of the Morphological and Physical Properties of Different Absorbent Wound Dressings
Sukhontha Hasatsri (2018)
10.1016/j.ijbiomac.2018.10.074
Investigating the effect of tetracycline addition on nanocomposite hydrogels based on polyvinyl alcohol and chitosan nanoparticles for specific medical applications.
P. Parsa (2019)
10.1016/j.ijbiomac.2017.08.142
Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing.
Haixia Xie (2018)
10.1002/ADMA.200701669
Dendrimer–Metallomacrocycle Composites: Nanofiber Formation by Multi‐Ion Pairing
P. Wang (2008)
10.1016/S0032-3861(00)00680-7
Transport properties of chitosan and whey blended with poly(ε-caprolactone) assessed by standard permeability measurements and microcalorimetry
I. Olabarrieta (2001)
10.1016/j.biomaterials.2015.02.076
Biomimetic LBL structured nanofibrous matrices assembled by chitosan/collagen for promoting wound healing.
R. Huang (2015)
10.1016/j.carbpol.2018.06.058
Hygroscopicity modulation of hydrogels based on carboxymethyl chitosan/Alginate polyelectrolyte complexes and its application as pH-sensitive delivery system.
Xiaojie Lv (2018)
10.1016/S0378-5173(01)00885-7
Effect of γ-radiation on a polyanhydride implant containing gentamicin sulfate
J. Deng (2002)
10.1016/j.msec.2018.04.077
Potential of silk sericin based nanofibrous mats for wound dressing applications.
Sween Gilotra (2018)
10.1039/c2pp25070e
Electrospun photosensitive nanofibers: potential for photocurrent therapy in skin regeneration.
Guorui Jin (2013)
10.1016/j.ajps.2018.04.004
Electrospun nanofibers as a wound dressing for treating diabetic foot ulcer
Y. Liu (2019)
10.1002/jbm.a.33010
Controlling the porosity of fibrous scaffolds by modulating the fiber diameter and packing density.
S. Soliman (2011)
10.1016/j.ijbiomac.2018.10.115
Wound dressing based on electrospun PVA/chitosan/starch nanofibrous mats: Fabrication, antibacterial and cytocompatibility evaluation and in vitro healing assay.
H. Adeli (2019)
10.5772/63961
Antimicrobial Dressings for Improving Wound Healing
Omar Sarheed (2016)
10.1016/J.POLYMER.2008.09.014
Use of electrospinning technique for biomedical applications
S. Agarwal (2008)
10.1039/C5RA18569F
A novel chitosan/wheat gluten biofoam fabricated by spontaneous mixing and vacuum-drying
F. Chen (2015)
10.1016/J.POLYMERTESTING.2017.06.023
In situ construction of Ag NPs in bio-inspired multilayer films for long-term bactericidal and biofilm inhibition properties
Bailiang Wang (2017)
10.1016/J.EURPOLYMJ.2014.09.007
Wheat gluten/chitosan blends: A new biobased material
F. Chen (2014)
10.1016/j.msec.2016.07.065
Ceramic scaffolds enriched with gentamicin loaded poly(lactide-co-glycolide) microparticles for prevention and treatment of bone tissue infections.
Łucja Rumian (2016)
10.1002/jbm.b.34199
Antibacterial 3D bone scaffolds for tissue engineering application.
Jitendra Pant (2019)
10.1016/J.POLYMERTESTING.2019.105955
Biomimetic electrospun polyurethane matrix composites with tailor made properties for bone tissue engineering scaffolds
S. K. Jaganathan (2019)
10.1016/j.msec.2015.10.005
Release behavior of tetracycline hydrochloride loaded chitosan/poly(lactic acid) antimicrobial nanofibrous membranes.
Suwei Jiang (2016)
10.1021/CM0484955
Electrospun polyacrylonitrile nanofibers containing a high concentration of well-aligned multiwall carbon nanotubes
Haoqing Hou (2005)
10.1021/JA048648P
Assembly of well-aligned multiwalled carbon nanotubes in confined polyacrylonitrile environments: electrospun composite nanofiber sheets.
J. Ge (2004)
10.1159/000446621
Electrospun Fibers for Drug Delivery after Spinal Cord Injury and the Effects of Drug Incorporation on Fiber Properties
C. Johnson (2016)
10.1016/J.MSEC.2019.03.111
Design and performance of sericin/poly(vinyl alcohol) hydrogel as a drug delivery carrier for potential wound dressing application.
G. Tao (2019)
10.1002/PAT.4563
Improved antibacterial properties of an Mg‐Zn‐Ca alloy coated with chitosan nanofibers incorporating silver sulfadiazine multiwall carbon nanotubes for bone implants
H. R. Bakhsheshi-Rad (2019)



This paper is referenced by
10.1016/j.polymertesting.2020.106872
Testing of fast dissolution of ibuprofen from its electrospun hydrophilic polymer nanocomposites
Yingfu Bai (2021)
10.3390/pharmaceutics12100983
An Overview of Biopolymeric Electrospun Nanofibers Based on Polysaccharides for Wound Healing Management
Andreea-Teodora Iacob (2020)
10.3390/polym12091926
Synthesis of Antibacterial Gelatin/Sodium Alginate Sponges and Their Antibacterial Activity
Yanyi Wen (2020)
10.3390/antiox9121309
Antioxidant, Antimicrobial and Antiviral Properties of Herbal Materials
Shokoh Parham (2020)
10.3390/ma13235388
Sustainable Rabbit Skin Glue to Produce Bioactive Nanofibers for Nonactive Wound Dressings
E. Matei (2020)
10.2147/IJN.S266692
Antimicrobial Double-Layer Wound Dressing Based on Chitosan/Polyvinyl Alcohol/Copper: In vitro and in vivo Assessment
Ensieh Ghasemian Lemraski (2021)
10.1016/j.cej.2020.126518
Exploiting synergistic effect of externally loaded bFGF and endogenous growth factors for accelerated wound healing using heparin functionalized PCL/gelatin co-spun nanofibrous patches
Akshat Joshi (2021)
10.3390/ma13112663
Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends
F. Pahlevanzadeh (2020)
10.3390/ma13183980
Recent Trends in Three-Dimensional Bioinks Based on Alginate for Biomedical Applications
F. Pahlevanzadeh (2020)
10.1016/j.xphs.2020.10.003
Delivery of therapeutics from layer-by-layer electrospun nanofiber matrix for wound healing: An update.
Tracey Anastacia Jeckson (2020)
10.1002/wnan.1626
2D and 3D electrospinning technologies for the fabrication of nanofibrous scaffolds for skin tissue engineering: A review.
Antonios Keirouz (2020)
10.3390/ma13092153
Electrospun Nano-Fibers for Biomedical and Tissue Engineering Applications: A Comprehensive Review
Shokoh Parham (2020)
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