Online citations, reference lists, and bibliographies.
← Back to Search

Long-term N Fertilization Imbalances Potential N Acquisition And Transformations By Soil Microbes.

L. Huang, C. Riggins, S. Rodríguez-Zas, M. C. Zabaloy, M. Villamil
Published 2019 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Nitrogen (N) fertilization in agricultural soils has been receiving worldwide attention due to its detrimental effects on ecosystem services, particularly on microbial N transformation. However, few studies provide a complete picture of N-fertilization effects on the N transformation cycle within a single agricultural ecosystem. Here, we explored the main steps of the microbial N cycle, using targeted gene abundances as proxies, in relation to soil properties, following 35 years of N-fertilization at increasing rates (0, 202 and 269 kg N/ha) in continuous corn (Zea mays L.) and corn-soybean [Glycine max (L.) Merr.] rotations. We used real-time quantitative polymerase chain reaction (qPCR) for the quantification of phylogenetic groups and functional gene screening of the soil microbial communities, including genes encoding critical enzymes of the microbial N cycle: nifH (N2 fixation), amoA (first step of nitrification), nirK and nirS (first step of denitrification), and nosZ (last step of denitrification). Our results showed that long term N-fertilization increased the abundance of fungal communities likely related to decreases in pH, and an enrichment of Al3+ and Fe3+ in exchange sites at the expense of critical macro and micronutrients. At the same time, long term N-fertilization damaged potential biological N2 fixation by significantly reducing the abundance of nifH genes in both continuous and rotated corn systems, while accelerating potential nitrification activities under continuous corn by increasing the abundance of bacterial amoA. Fertilization did not affect the abundance of denitrifying groups. Altogether, these results suggest that N fertilization in corn crops potentially decreases N2 acquisition by free-living soil microbes and stimulates nitrification activities, thus creating a vicious loop that makes the overall agricultural system even more dependent on external N inputs.
This paper references
Role of carbon, nitrogen, and sulfur cycles in soil acidification.
N. Bolan (2003)
Nitrate reduction and the nitrogen cycle in archaea.
P. Cabello (2004)
Nitrogen enrichment shifts functional genes related to nitrogen and carbon acquisition in the fungal community
K. Treseder (2018)
Edward E. Gbur (2012)
Restoring Soil Quality to Mitigate Soil Degradation
R. Lal (2015)
Microbial diversity affects self-organization of the soil–microbe system with consequences for function
J. Crawford (2011)
Long-term crop rotation and tillage effects on soil greenhouse gas emissions and crop production in Illinois, USA
G. D. Behnke (2018)
Structure and function of the global topsoil microbiome
M. Bahram (2018)
Short Corn Rotations Do Not Improve Soil Quality, Compared with Corn Monocultures
M. Hoss (2018)
Growth, activity and temperature responses of ammonia-oxidizing archaea and bacteria in soil microcosms.
Maria Tourna (2008)
Fungal and bacterial growth responses to N fertilization and pH in the 150-year 'Park Grass' UK grassland experiment.
J. Rousk (2011)
Changes in soil phosphorus fractions after 9 years of continuous nitrogen addition in a Larix gmelinii plantation
K. Yang (2014)
The effect of phosphorus deficiency on nutrient uptake, nitrogen fixation and photosynthetic rate in mashbean, mungbean and soybean
M. I. Chaudhary (2008)
Different denitrification potential of aquic brown soil in Northeast China under inorganic and organic fertilization accompanied by distinct changes of nirS- and nirK-denitrifying bacterial community
C. Yin (2014)
Fertilizer source and tillage effects on yield-scaled nitrous oxide emissions in a corn cropping system.
R. Venterea (2011)
Global patterns of crop yield stability under additional nutrient and water inputs
C. Müller (2018)
Agriculture in the Midwest
J. Hatfield (2012)
Keys to Soil Taxonomy
Anònim Anònim (2010)
Quantitative Detection of the nosZ Gene, Encoding Nitrous Oxide Reductase, and Comparison of the Abundances of 16S rRNA, narG, nirK, and nosZ Genes in Soils
S. Henry (2006)
Metagenomic analyses reveal no differences in genes involved in cellulose degradation under different tillage treatments.
Maria de Vries (2015)
Impact of 36 years of nitrogen fertilization on microbial community composition and soil carbon cycling-related enzyme activities in rhizospheres and bulk soils in northeast China
Qing-feng Wang (2019)
Nutrient limitation of terrestrial free-living nitrogen fixation.
Katherine A. Dynarski (2018)
Significant Acidification in Major Chinese Croplands
J. Guo (2010)
Cell biology and molecular basis of denitrification.
W. Zumft (1997)
A global analysis of soil acidification caused by nitrogen addition
Dashuan Tian (2015)
Exploring the Relationships between Greenhouse Gas Emissions, Yields, and Soil Properties in Cropping Systems
G. D. Behnke (2018)
Field management effects on soil enzyme activities
A. K. Bandick (1999)
Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil.
F. Poly (2001)
Investigation of the role of phosphorus in symbiotic dinitrogen fixation.
D. Israel (1987)
The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations.
J. H. Rotthauwe (1997)
Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients
N. Fierer (2012)
Soil chemical properties affecting NH4+ sorption in forest soils
G. Matschonat (1996)
Interactive influence of phosphorus and iron on nitrogen fixation by soybean
V. Rotaru (2009)
Bacterial gene abundances as indicators of greenhouse gas emission in soils
S. E. Morales (2010)
Closing yield gaps through nutrient and water management
N. Mueller (2012)
Meta-analysis reveals ammonia-oxidizing bacteria respond more strongly to nitrogen addition than ammonia-oxidizing archaea
C. Carey (2016)
The consequences of niche and physiological differentiation of archaeal and bacterial ammonia oxidisers for nitrous oxide emissions
Linda Hink (2017)
Chapter 14: Multivariate Methods for Agricultural Research
K. Yeater (2018)
Chapter 14 – Nitrogen Transformations
G. P. Robertson (2015)
Carbon and Nitrogen Amendments Lead to Differential Growth of Bacterial and Fungal Communities in a High-pH Soil
P. N. Kamble (2018)
Biological nitrogen fixation: rates, patterns and ecological controls in terrestrial ecosystems
P. Vitousek (2013)
Archaea produce lower yields of N2O than bacteria during aerobic ammonia oxidation in soil
Linda Hink (2017)
North American Soil Degradation: Processes, Practices, and Mitigating Strategies
R. L. Baumhardt (2015)
Effect of nitrogen fertilization on the abundance of nitrogen cycling genes in agricultural soils: A meta-analysis of field studies
Yang Ouyang (2018)
Detection and analysis of elusive members of a novel and diverse archaeal community within a thermal spring streamer consortium
D. Colman (2014)
Abundance of microbial genes associated with nitrogen cycling as indices of biogeochemical process rates across a vegetation gradient in Alaska.
D. G. Petersen (2012)
Effects of new coated release fertilizer on the growth of maize
Y. Dong (2016)
Different roles of rhizosphere effect and long-term fertilization in the activity and community structure of ammonia oxidizers in a calcareous fluvo-aquic soil
Chao Ai (2013)
R: A language and environment for statistical computing.
R. Team (2014)
Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR.
S. Henry (2004)
Impact of 25 years of inorganic fertilization on diazotrophic abundance and community structure in an acidic soil in southern China
Wang Chao (2017)
Crop Rotation and Tillage Effects on Soil Physical and Chemical Properties in Illinois
S. Zuber (2015)
Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria
W. Martens-Habbena (2009)
Soil metagenomics reveals differences under conventional and no-tillage with crop rotation or succession
Renata Carolini Souza (2013)
Bacterial Indicator of Agricultural Management for Soil under No-Till Crop Production
E. L. Figuerola (2012)
pH-decrease in nitrifying biofilms
H. Szwerinski (1986)
An Underground Revolution: Biodiversity and Soil Ecological Engineering for Agricultural Sustainability.
S. F. Bender (2016)
Long-term n fertilization affects soil bacterial communities in agronomic fields
Renpeng Sun (2018)
Contrasting Soil pH Effects on Fungal and Bacterial Growth Suggest Functional Redundancy in Carbon Mineralization
J. Rousk (2009)
The Importance of the Microbial N Cycle in Soil for Crop Plant Nutrition.
P. Hirsch (2015)
Soil acidification and the importance of liming agricultural soils with particular reference to the United Kingdom
K. Goulding (2016)
Adaptation of soil nitrifiers to very low nitrogen level jeopardizes the efficiency of chemical fertilization in west african moist savannas
Féline L. Assémien (2017)
Response of the Abundance of Key Soil Microbial Nitrogen-Cycling Genes to Multi-Factorial Global Changes
X. Zhang (2013)
Microbial functional genes involved in nitrogen fixation, nitrification and denitrification in forest ecosystems
D. Levy-Booth (2014)
How do phosphorus, potassium and sulphur affect plant growth and biological nitrogen fixation in crop and pasture legumes? A meta-analysis
G. Divito (2014)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar