Online citations, reference lists, and bibliographies.
← Back to Search

Sphingosine-1-phosphate-induced Flk-1 Transactivation Stimulates Mouse Embryonic Stem Cell Proliferation Through S1P1/S1P3-dependent β-arrestin/c-Src Pathways.

J. M. Ryu, Young Bin Baek, Myung Sun Shin, Ji Hoon Park, Soo-hyun Park, J. Lee, H. Han
Published 2014 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Although recent findings showed that the bioactive lipid metabolites can regulate the ES cell functions, the physiological relevance of interaction between sphingosine-1-phosphate (S1P) and Flk-1 and its related signaling molecules are not yet clear in ES cell proliferation. In the present study, S1P1-5 receptors were expressed in mouse ES cells and S1P increased S1P1-3 receptor expression level. S1P treatment stimulated the cellular proliferation in S1P1/3-dependent manner, located in lipid rafts. In response to S1P, β-arrestin was recruited to S1P1/3 receptor and c-Src was activated. S1P also increased the binding of S1P1/3 receptor with Flk-1. Similar to responses for VEGF, S1P increased Flk-1 phosphorylation, which was blocked by β-arrestin siRNA, and PP2, but not by VEGF-A164 antibody or VEGF siRNA. In addition, S1P induced VEGF expression and VEGFR2 kinase inhibitor (SU1498) blocked the S1P-induced cellular proliferation. However, VEGF-A164 antibody or VEGF siRNA partially blocked S1P-induced cellular proliferation, suggesting that both VEGF-dependent Flk-1 activation and VEGF-independent Flk-1 activation are involved in S1P-induced ES cell proliferation. S1P and VEGF-induced phosphorylation of ERK and JNK were blocked by pretreatment with SU1498. Moreover, inhibition of ERK and JNK blocked S1P-induced cellular proliferation. In conclusion, S1P-elicited transactivation of Flk-1 mediated by S1P1/3-dependent β-arrestin/c-Src pathways stimulated mouse ES cell proliferation.
This paper references
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1084/JEM.174.6.1517
Lipopolysaccharide-mediated transcriptional activation of the human tissue factor gene in THP-1 monocytic cells requires both activator protein 1 and nuclear factor kappa B binding sites
N. Mackman (1991)
10.1074/JBC.271.16.9690
Co-purification and Direct Interaction of Ras with Caveolin, an Integral Membrane Protein of Caveolae Microdomains
Kenneth S. Song (1996)
10.1093/OXFORDJOURNALS.JBCHEM.A021681
Sphingosine 1-phosphate, a bioactive sphingolipid abundantly stored in platelets, is a normal constituent of human plasma and serum.
Y. Yatomi (1997)
10.1101/GAD.13.12.1501
CDK inhibitors: positive and negative regulators of G1-phase progression.
C. Sherr (1999)
10.1080/713803698
Sphingosine 1‐Phosphate Activates Erk‐1/‐2 by Transactivating Epidermal Growth Factor Receptor in Rat‐2 Cells
Joohee Kim (2000)
10.1172/JCI10905
Edg-1, the G protein-coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation.
Y. Liu (2000)
10.1126/SCIENCE.290.5496.1574
Beta-arrestin 2: a receptor-regulated MAPK scaffold for the activation of JNK3.
P. McDonald (2000)
10.1247/CSF.26.137
Molecular mechanism to maintain stem cell renewal of ES cells.
H. Niwa (2001)
10.1074/JBC.M204764200
Transactivation of Vascular Endothelial Growth Factor (VEGF) Receptor Flk-1/KDR Is Involved in Sphingosine 1-Phosphate-stimulated Phosphorylation of Akt and Endothelial Nitric-oxide Synthase (eNOS)*
T. Tanimoto (2002)
The role of beta-arrestins in the termination and transduction of G-protein-coupled receptor signals.
L. Luttrell (2002)
10.1042/BST0311216
Exogenous and intracellularly generated sphingosine 1-phosphate can regulate cellular processes by divergent pathways.
S. Spiegel (2003)
10.1186/1471-2199-4-6
The kinase MSK1 is required for induction of c-fos by lysophosphatidic acid in mouse embryonic stem cells
S. Schuck (2003)
10.1038/sj.bjp.0705055
Regulated and constitutive activation of specific signalling pathways by the human S1P5 receptor
Anke Niedernberg (2003)
10.1074/JBC.M208560200
Sphingosine 1-Phosphate and Platelet-derived Growth Factor (PDGF) Act via PDGFβ Receptor-Sphingosine 1-Phosphate Receptor Complexes in Airway Smooth Muscle Cells*
C. Waters (2003)
10.1023/A:1018948608211
Interaction of Nucleoside Analogues with the Sodium–Nucleoside Transport System in Brush Border Membrane Vesicles from Human Kidney
C. Brett (2004)
10.1016/J.CRYOBIOL.2003.12.001
Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm.
P. H. Purdy (2004)
10.1196/annals.1349.011
The Role of Sphingosine 1‐Phosphate Receptors in the Trafficking of Hematopoietic Progenitor Cells
Gabriele Seitz (2005)
10.1128/MCB.25.24.11113-11121.2005
Essential Role for Sphingosine Kinases in Neural and Vascular Development
K. Mizugishi (2005)
10.1038/nri1650
Sphingosine 1-phosphate and its receptors: an autocrine and paracrine network
H. Rosen (2005)
10.1634/stemcells.2004-0338
Essential Roles of Sphingosine‐1‐Phosphate and Platelet‐Derived Growth Factor in the Maintenance of Human Embryonic Stem Cells
Alice Pébay (2005)
10.1016/J.BBRC.2005.05.200
Cell cycle synchronization of embryonic stem cells: effect of serum deprivation on the differentiation of embryonic bodies in vitro.
E. Zhang (2005)
10.1096/fj.05-3730fje
Type 4 sphingosine 1‐phosphate G protein‐coupled receptor (S1P4) transduces S1P effects on T cell proliferation and cytokine secretion without signaling migration
W. Wang (2005)
10.1074/jbc.M603680200
Transactivation of Sphingosine 1-Phosphate Receptors Is Essential for Vascular Barrier Regulation
P. Singleton (2006)
10.1089/SCD.2006.15.729
The regulation of self-renewal in human embryonic stem cells.
Stuart Avery (2006)
10.1074/jbc.M605339200
Insulin-like Growth Factors Mediate Heterotrimeric G Protein-dependent ERK1/2 Activation by Transactivating Sphingosine 1-Phosphate Receptors*
H. M. El-Shewy (2006)
10.1089/SCD.2006.15.789
Mediation of apoptosis and proliferation of human embryonic stem cells by sphingosine-1-phosphate.
Katie Inniss (2006)
10.2174/138161206775193109
Lysophospholipid receptors as potential drug targets in tissue transplantation and autoimmune diseases.
J. Chun (2006)
10.1016/J.CELLSIG.2006.07.015
The bioactive lipid sphingosylphosphorylcholine induces differentiation of mouse embryonic stem cells and human promyelocytic leukaemia cells.
A. Kleger (2007)
10.1634/stemcells.2006-0725
Sphingosine 1‐Phosphate Mediates Proliferation and Survival of Mesoangioblasts
C. Donati (2007)
10.1089/SCD.2007.0057
Anti-apoptotic effect of sphingosine-1-phosphate and platelet-derived growth factor in human embryonic stem cells.
R. Wong (2007)
10.1016/J.BIOCEL.2007.04.010
Vascular endothelial growth factor: biology and therapeutic applications.
Q. T. Ho (2007)
10.1016/J.BBAMEM.2006.09.026
Lysophospholipid receptors: signalling, pharmacology and regulation by lysophospholipid metabolism.
D. Meyer Zu Heringdorf (2007)
10.1038/sj.cr.7310126
Upregulation of Flk-1 by bFGF via the ERK pathway is essential for VEGF-mediated promotion of neural stem cell proliferation
Z. Xiao (2007)
10.1074/jbc.R600028200
Functions of the Multifaceted Family of Sphingosine Kinases and Some Close Relatives*
S. Spiegel (2007)
10.1016/j.cell.2008.02.008
Differentiation of Embryonic Stem Cells to Clinically Relevant Populations: Lessons from Embryonic Development
C. Murry (2008)
10.1038/nrm2329
Principles of bioactive lipid signalling: lessons from sphingolipids
Y. Hannun (2008)
10.1016/j.cellsig.2009.09.002
Phosphotyrosine protein dynamics in cell membrane rafts of sphingosine-1-phosphate-stimulated human endothelium: role in barrier enhancement.
Jing Zhao (2009)
10.1159/000231891
Regulation of Stem Cell Pluripotency and Neural Differentiation by Lysophospholipids
S. Pitson (2009)
10.1152/ajpcell.00579.2008
Arachidonic acid potentiates hypoxia-induced VEGF expression in mouse embryonic stem cells: involvement of Notch, Wnt, and HIF-1alpha.
S. Lee (2009)
10.1089/scd.2009.0023
Sphingosine 1-phosphate regulation of extracellular signal-regulated kinase-1/2 in embryonic stem cells.
A. Rodgers (2009)
10.1194/jlr.M001545
Lipid rafts play an important role for maintenance of embryonic stem cell self-renewal[S]
M. Lee (2010)
10.1016/j.exphem.2010.05.002
MT1-MMP association with membrane lipid rafts facilitates G-CSF--induced hematopoietic stem/progenitor cell mobilization.
N. Shirvaikar (2010)
10.1016/j.bbrc.2010.04.019
Sphingosine-1-phosphate induces human endothelial VEGF and MMP-2 production via transcription factor ZNF580: novel insights into angiogenesis.
Hui-yan Sun (2010)
10.14670/HH-26.1057
Isolation of pluripotent stem cells from human third molar dental pulp.
M. Atari (2011)
10.1002/stem.615
LacdiNAc (GalNAcβ1‐4GlcNAc) Contributes to Self‐Renewal of Mouse Embryonic Stem Cells by Regulating Leukemia Inhibitory Factor/STAT3 Signaling
Norihiko Sasaki (2011)
10.1242/jcs.076794
Sphingosine 1-phosphate regulates matrix metalloproteinase-9 expression and breast cell invasion through S1P3–Gαq coupling
Eun-sook Kim (2011)
10.1242/jcs.099044
Sphingosine-1-phosphate-induced release of TIMP-2 from vascular smooth muscle cells inhibits angiogenesis
Keith S. Mascall (2012)
10.1016/j.bbamcr.2012.06.011
Sphingosine 1-phosphate induces MKP-1 expression via p38 MAPK- and CREB-mediated pathways in airway smooth muscle cells.
W. Che (2012)
10.1152/ajpheart.00739.2011
Novel role of p66Shc in ROS-dependent VEGF signaling and angiogenesis in endothelial cells.
Jin Oshikawa (2012)
10.1074/jbc.M112.416552
Regulation of Autophagy and Its Associated Cell Death by “Sphingolipid Rheostat”
Makoto Taniguchi (2012)



This paper is referenced by
10.1016/j.cellsig.2021.109929
Lipid rafts as platforms for sphingosine 1-phosphate metabolism and signalling.
Chiara D'Aprile (2021)
10.1002/jcp.29958
Recent advances of the function of sphingosine 1‐phosphate (S1P) receptor S1P3
Xuehui Fan (2021)
10.1002/stem.3145
Sphingosine kinases protect murine embryonic stem cells from sphingosine‐induced cell cycle arrest
Suveg Pandey (2020)
10.1038/s41598-020-70896-0
Follicle-stimulating hormone promotes the proliferation of epithelial ovarian cancer cells by activating sphingosine kinase
Keqi Song (2020)
10.1002/glia.23803
Sphingosine 1‐phosphate promotes the proliferation of olfactory ensheathing cells through YAP signaling and participates in the formation of olfactory nerve layer
Xiao-mei Bao (2020)
10.1016/j.heares.2019.107839
Vascular endothelial growth factor is required for regeneration of auditory hair cells in the avian inner ear
Liangcai Wan (2020)
10.1177/2045894020905521
Sphingosine-1-phosphate receptor-independent lung endothelial cell barrier disruption induced by FTY720 regioisomers
S. Camp (2020)
10.3390/ijms21239310
The Role of β-Arrestins in Regulating Stem Cell Phenotypes in Normal and Tumorigenic Cells
G. Kallifatidis (2020)
10.1007/s10456-019-09676-y
VEGFR2 activation mediates the pro-angiogenic activity of BMP4
S. Rezzola (2019)
10.1007/s00403-019-01961-6
Association between sphingosine-1-phosphate-induced signal transduction via mitogen-activated protein kinase pathways and keloid formation
S. Jung (2019)
10.1016/J.ANDO.2019.06.003
Sphingosine-1-phosphate (S1P) in ovarian physiology and disease.
C. G. Hernández-Coronado (2019)
10.1016/j.plipres.2018.09.001
Roles of lysophosphatidic acid and sphingosine-1-phosphate in stem cell biology.
Grace E. Lidgerwood (2018)
10.1007/7651_2017_43
Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation.
G. Wang (2018)
10.1007/s00441-018-2792-3
Combined use of bone marrow-derived mesenchymal stromal cells (BM-MSCs) and platelet rich plasma (PRP) stimulates proliferation and differentiation of myoblasts in vitro: new therapeutic perspectives for skeletal muscle repair/regeneration
C. Sassoli (2018)
10.3390/ijms19020420
Expansion of Sphingosine Kinase and Sphingosine-1-Phosphate Receptor Function in Normal and Cancer Cells: From Membrane Restructuring to Mediation of Estrogen Signaling and Stem Cell Programming
O. Sukocheva (2018)
10.1124/mol.116.107854
GABABR-Induced EGFR Transactivation Promotes Migration of Human Prostate Cancer Cells
S. Xia (2017)
10.1007/978-3-319-49343-5_8
The Emerging Role of Sphingolipids in Cancer Stem Cell Biology
A. C. Lewis (2017)
10.1097/FJC.0000000000000482
G Protein–Coupled Receptor Signaling Through &bgr;-Arrestin–Dependent Mechanisms
Pierre-Yves Jean-Charles (2017)
10.1038/s41598-017-04175-w
Palmitic Acid-BSA enhances Amyloid-β production through GPR40-mediated dual pathways in neuronal cells: Involvement of the Akt/mTOR/HIF-1α and Akt/NF-κB pathways
J. Y. Kim (2017)
10.1007/978-3-319-49343-5_2
Morphogenetic Sphingolipids in Stem Cell Differentiation and Embryo Development
G. Wang (2017)
10.1007/978-3-319-49343-5_1
Lysophosphatidic Acid and Sphingosine-1-Phosphate in Pluripotent Stem Cells
Grace E. Lidgerwood (2017)
10.1038/s41598-017-13471-4
Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation
H. Li (2017)
10.3390/molecules22030344
Sphingosine 1-Phosphate Receptor 1 Signaling in Mammalian Cells
N. Pyne (2017)
10.1016/j.yexcr.2017.04.023
Downregulation of &bgr;‐arrestin 1 suppresses glioblastoma cell malignant progression vis inhibition of Src signaling
T. Lan (2017)
10.1152/ajpcell.00148.2016
Shear stress induces Gαq/11 activation independently of G protein-coupled receptor activation in endothelial cells.
Nathaniel G dela Paz (2017)
10.1007/978-3-319-49343-5
Lipidomics of Stem Cells
Alice Pébay (2017)
10.1167/iovs.16-20684
Sphingosine-1-Phosphate Mediates Fibrosis in Orbital Fibroblasts in Graves' Orbitopathy.
Jaesang Ko (2017)
10.1016/J.COLSURFA.2016.04.027
On the possible structural role of single chain sphingolipids Sphingosine and Sphingosine 1-phosphate in the amyloid-β peptide interactions with membranes. Consequences for Alzheimer’s disease development
Chiho Watanabe (2016)
10.7939/R3542JK56
Thinking inside and outside the blood vessel: S1P-mediated control of vascular tone and the impact of CMV infection
D. Kerage (2016)
10.1152/ajpheart.00807.2015
Carvedilol-responsive microRNAs, miR-199a-3p and -214 protect cardiomyocytes from simulated ischemia-reperfusion injury.
Kyoung-mi Park (2016)
10.1002/jcp.24734
VEGF Receptor 2 (VEGFR2) Activation Is Essential for Osteocyte Survival Induced by Mechanotransduction
Luis F de Castro (2015)
10.1002/stem.1882
Autotaxin‐LPA Axis Regulates hMSC Migration by Adherent Junction Disruption and Cytoskeletal Rearrangement Via LPAR1/3‐Dependent PKC/GSK3β/β‐Catenin and PKC/Rho GTPase Pathways
J. Ryu (2015)
See more
Semantic Scholar Logo Some data provided by SemanticScholar