← Back to Search
A Light Scattering And Fluorescence Emission Coupled Ratiometry Using The Interaction Of Functional CdS Quantum Dots With Aminoglycoside Antibiotics As A Model System.
Qie Gen Liao, Y. Li, C. Huang
Published 2007 · Medicine, Chemistry
Download PDFAnalyze on Scholarcy
Any signals, if their intensities have simple functional relationship with analyte concentration, can be applied to analytical purposes. Rayleigh light scattering signals and fluorescence signals are twins in flurospectroscopy, so the light scattering signals are the major interference when the Stokes shift is small. Herein, we propose a light scattering and fluorescence emission (LS-FL) coupled ratiometry using CdS quantum dots (QDs) as a fluorescence probe to detect aminoglycoside antibiotics (AGs). As model analytes, AGs, when attached to the surface of CdS-QDs via electrostatic interaction in aqueous medium, result in strong enhanced light scattering (LS) emission characterized at 376nm and fluorescence quenching of CdS-QDs at 500nm. Thus, a ratiometry using the coexistent light scattering and fluorescent emission signals has been proposed. Based on the linear relationship between logarithm of light scattering and fluorescence emission ratio (R) and logarithm of AGs concentration, a novel assay of AGs is established with the limits of detection (3sigma) being 58-190nmol l(-1), and applied successfully to detect AGs injection and serum samples.
This paper references
Dual emission probe for luminescence oxygen sensing: a critical comparison between intensity, lifetime and ratiometric measurements.
Hannes Hochreiner (2005)
Flow injection fluorescence immunoassay for gentamicin using sol-gel-derived mesoporous biomaterial.
H. Yang (2002)
Laboratory guidelines for monitoring of antimicrobial drugs
C. Hammett-Stabler (1998)
Photochemistry of colloidal semiconductors. 20. Surface modification and stability of strong luminescing CdS particles
L. Spanhel (1987)
Non-derivatization approach to high-performance liquid chromatography-fluorescence detection for aminoglycoside antibiotics based on a ligand displacement reaction.
M. Yang (2001)
Wavelength-ratiometric near-physiological pH sensors based on 6-aminoquinolinium boronic acid probes.
R. Badugu (2005)
Quantum dots-based reverse phase protein microarray.
Masato Shingyoji (2005)
Determination of nanograms of nucleic acids by their enhancement effect on the resonance light scattering of the cobalt(II)/4-[(5-chloro-2-pyridyl)azo]-1,3-diaminobenzene complex.
C. Huang (1997)
Pulmonary retention of free and liposome-encapsulated tobramycin after intratracheal administration in uninfected rats and rats infected with Pseudomonas aeruginosa.
Abdelwahab Omri (1994)
A resonance light scattering ratiometry applied for binding study of organic small molecules with biopolymer.
C. Huang (2006)
A ratiometric fluorescence probe for selective visual sensing of Zn2+.
A. Ajayaghosh (2005)
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
G. Grynkiewicz (1985)
Activities of Tobramycin and Six Other Antibiotics against Pseudomonas aeruginosa Isolates from Patients with Cystic Fibrosis
R. Shawar (1999)
Topics in Fluorescence Spectroscopy
J. Lakowicz (2002)
Chemiluminescence of CdTe nanocrystals induced by direct chemical oxidation and its size-dependent and surfactant-sensitized effect.
Z. Wang (2005)
High-sensitivity detection of DNA hybridization on microarrays using resonance light scattering.
Paul G. Bao (2002)
Reversible ratiometric probe for quantitative DNA measurements.
J. Ueberfeld (2004)
Single biosensor immunoassay for the detection of five aminoglycosides in reconstituted skimmed milk
W. Haasnoot (2003)
Determination of Nucleic Acids by a Resonance Light-Scattering Technique with α,β,γ,δ-Tetrakis[4- (trimethylammoniumyl)phenyl]porphine
C. Huang (1996)
Luminescent CdS quantum dots as selective ion probes.
Y. Chen (2002)
A sensitive method for the detection of catecholamine based on fluorescence quenching of CdSe nanocrystals.
Ying Ma (2005)
Microarray-based detection of protein binding and functionality by gold nanoparticle probes.
Zhenxin Wang (2005)
Porphyrin assembly on beta-cyclodextrin for selective sensing and detection of a zinc ion based on the dual emission fluorescence ratio.
R. Yang (2003)
L. J. Yu (2002)
Porphyrin Assemblies On DNA As Studied By A Resonance Light-Scattering Technique
R. Pasternack (1993)
J. Ueberfeld (2004)
G. Crynkiewicz (1985)
Y. J. Long (2005)
Lakowicz (Ed.), Topics in Fluorescence Spectroscopy
J. R. Lakowicz (1994)
R. H. Yang (2003)
CdSe quantum dots as luminescent probes for spironolactone determination.
Jiangong Liang (2006)
Angular-ratiometric plasmon-resonance based light scattering for bioaffinity sensing.
K. Aslan (2005)
Towards single-spot multianalyte molecular beacon biosensors.
Christopher M. Strohsahl (2005)
Electronic wave functions in semiconductor clusters: experiment and theory
L. Brus (1986)
Synthesis and Characterization of Strongly Luminescing ZnS-Capped CdSe Nanocrystals
Margaret A. Hines and (1996)
C. Z. Huang (1996)
A wide dynamic range detection of biopolymer medicines with resonance light scattering and absorption ratiometry
Y. Long (2005)
Fluorescence resonance energy transfer in doubly-quantum dot labeled IgG system.
Q. Ma (2005)
This paper is referenced by
Binding of daunorubicin to human serum albumin using molecular modeling and its analytical application.
F. Cui (2008)
Study on the interaction between CdSe quantum dots and chitosan by scattering spectra.
Juanjuan Peng (2009)
Synchronous fluorescence determination of mercury ion with glutathione-capped CdS nanoparticles as a fluorescence probe.
A-Ni Liang (2010)
A facile and label-free ratiometric optical sensor for selective detection of norepinephrine by combining second-order scattering and fluorescence signals
Y. Zhang (2019)
Simple and sensitive determination of papain by resonance light-scattering with CdSe quantum dots.
S. Huang (2013)
Highly sensitive determination of poly(hexamethylene guanidine) by Rayleigh scattering using aggregation of silver nanoparticles
A. Artemyeva (2014)
Synchronous fluorescence determination and molecular modeling of 5-Aminosalicylic acid (5-ASA) interacted with human serum albumin
F. Cui (2008)
High-sensitivity detection of polysaccharide using phosphodiesters quaternary ammonium salt as probe by decreased resonance light scattering.
Zhanguang Chen (2009)
Graphene quantum dots and the resonance light scattering technique for trace analysis of phenol in different water samples.
Ruiling Sun (2014)
Dextran-coated CdSe quantum dots for the optical detection of monosaccharides by resonance light-scattering technique
Shuang Hu (2010)
Effect of dextran molecular weight on resonance light-scattering of quantum dots modified with dextran and concanavalin A
Shuang Hu (2011)
Nanocatalytic resonance scattering spectral analysis
Zhiliang Jiang (2010)
Layer-by-layer self-assembly xylenol orange functionalized CdSe/CdS quantum dots as a turn-on fluorescence lead ion sensor.
Q. Zhao (2013)
Low-Dimension Nanomaterial-Based Sensing Matrices for Antibiotics Detection: A Mini Review
Yucan Dong (2020)
Coomassie brilliant blue R-250 as a new surface-enhanced Raman scattering probe for prion protein through a dual-aptamer mechanism.
P. Hu (2015)
Highly sensitive synchronous fluorescence determination of mercury (II) based on the denatured ovalbumin coated CdTe QDs.
Yan-Qin Wang (2012)
Dual-aptamer-based sensitive and selective detection of prion protein through the fluorescence resonance energy transfer between quantum dots and graphene oxide
S. Zhen (2013)
Recent Developments of the Resonance Light Scattering Technique: Technical Evolution, New Probes and Applications
Jian Ling (2007)
Microarray Technology for Major Chemical Contaminants Analysis in Food: Current Status and Prospects
Zhaowei Zhang (2012)
Studying the interaction between CdTe quantum dots and Nile blue by absorption, fluorescence and resonance Rayleigh scattering spectra.
Juan-Juan Peng (2010)
Determination of Aspirin Using Functionalized Cadmium-Tellurium Quantum Dots as a Fluorescence Probe
H. Li (2015)
Resonance Rayleigh-scattering spectral method for the determination of some aminoglycoside antibiotics using CdTe quantum dots as a probe.
L. Wang (2010)
Ultra-sensitive detection of prion protein with a long range resonance energy transfer strategy.
P. Hu (2010)
Light-scattering signals from nanoparticles in biochemical assay, pharmaceutical analysis and biological imaging
J. Ling (2009)
Critical approach to synchronous spectrofluorimetry. II
A. Andrade-Eiroa (2010)