← Back to Search
Relevance And Regulation Of Cell Density.
Gabriel E Neurohr, A. Amon
Published 2020 · Biology, Medicine
Download PDFAnalyze on Scholarcy
Cell density shows very little variation within a given cell type. For example, in humans variability in cell density among cells of a given cell type is 100 times smaller than variation in cell mass. This tight control indicates that maintenance of a cell type-specific cell density is important for cell function. Indeed, pathological conditions such as cellular senescence are accompanied by changes in cell density. Despite the apparent importance of cell-type-specific density, we know little about how cell density affects cell function, how it is controlled, and how it sometimes changes as part of a developmental process or in response to changes in the environment. The recent development of new technologies to accurately measure the cell density of single cells in suspension and in tissues is likely to provide answers to these important questions.
This paper references
Translational control of lipogenic enzymes in the cell cycle of synchronous, growing yeast cells
Heidi M Blank (2017)
Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine
J. Cheng (2015)
Studying protein dynamics in living cells
J. Lippincott-Schwartz (2001)
Buoyant density variation during the cell cycle of Saccharomyces cerevisiae.
W. Baldwin (1984)
Separation of subcellular components and viruses by combined rate- and isopycnic-zonal centrifugation.
Anderson Ng (1966)
Microtubule assembly nucleated by isolated centrosomes
T. Mitchison (1984)
A Coordinated Global Control over Cellular Transcription
J. Zhurinsky (2010)
Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis
Sungmin Son (2015)
Trehalose Is a Versatile and Long-Lived Chaperone for Desiccation Tolerance
Hugo Tapia (2014)
Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis.
E. Maeno (2000)
Mitogen-activated protein kinases as key players in osmotic stress signaling.
Xiangyu Zhou (2016)
Growth and metabolism of inositol-starved Saccharomyces cerevisiae.
S. Henry (1977)
Actin polymerization and ATP hydrolysis.
M. Carlier (1990)
Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae
P. Novick (1979)
The Centrosome Is a Selective Condensate that Nucleates Microtubules by Concentrating Tubulin
Jeffrey B Woodruff (2017)
Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy.
Dan Fu (2012)
Mediator and RNA polymerase II clusters associate in transcription-dependent condensates
Won-Ki Cho (2018)
Excessive Cell Growth Causes Cytoplasm Dilution And Contributes to Senescence
Gabriel E Neurohr (2019)
Life and Death of Lymphocytes: A Volume Regulation Affair
C. Bortner (2011)
Desiccation tolerance: an unusual window into stress biology
D. Koshland (2019)
SEPARATION OF HELA CELLS BY COLLOIDAL SILICA DENSITY GRADIENT CENTRIFUGATION
D. Wolff (1972)
Physiology of the yeast Kluyveromyces marxianus during batch and chemostat cultures with glucose as the sole carbon source.
G. Fonseca (2007)
Regulated Formation of an Amyloid-like Translational Repressor Governs Gametogenesis
Luke E. Berchowitz (2015)
High Viscosity and Anisotropy Characterize the Cytoplasm of Fungal Dormant Stress-Resistant Spores
J. Dijksterhuis (2006)
Dilution of the cell cycle inhibitor Whi5 controls budding yeast cell size
K. Schmoller (2015)
Osmotic Properties of Human Red Cells
D. Savitz (1964)
The effect of macromolecular crowding on protein aggregation and amyloid fibril formation
L. Munishkina (2004)
Leukocyte density and volume in normal subjects and in patients with acute lymphoblastic leukemia.
A. Zipursky (1976)
Fluorescence recovery after photobleaching in material and life sciences: putting theory into practice.
N. Loren (2015)
Functional transcription promoters at DNA double-strand breaks mediate RNA-driven phase separation of damage response factors
F. Pessina (2019)
Physiology of the bacterial cell
F. C. Neidhardt (1990)
Glass formation and dormancy in bacterial spores
S. Ablett (1999)
Fluorescent measurement of the intracellular pH during sporulation of Saccharomyces cerevisiae.
J. Aon (1997)
Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under different conditions of glucose repression.
A. Gombert (2001)
Measurement of the internal pH of yeast spores by 31P nuclear magnetic resonance.
J. Barton (1980)
Multiple Phases of Chondrocyte Enlargement Underlie Differences in Skeletal Proportions
K. Cooper (2013)
Dehydration partitioned within core protoplast accounts for heat resistance of bacterial spores
T. C. Beaman (1984)
Fractionation of Escherichia coli cell populations at different stages during growth transition to stationary phase
Hideki Makinoshima (2002)
In Situ Cryo-Electron Tomography: A Post-Reductionist Approach to Structural Biology.
Shoh Asano (2016)
The Bacterial Cytoplasm Has Glass-like Properties and Is Fluidized by Metabolic Activity
Bradley R. Parry (2014)
Flux distributions in anaerobic, glucose-limited continuous cultures of Saccharomyces cerevisiae.
T. L. Nissen (1997)
Reciprocal Phosphorylation of Yeast Glycerol-3-Phosphate Dehydrogenases in Adaptation to Distinct Types of Stress
Y. J. Lee (2012)
Quantitative Analysis of Fission Yeast Transcriptomes and Proteomes in Proliferating and Quiescent Cells
S. Marguerat (2012)
Measurement of mass, density, and volume during the cell cycle of yeast
A. Bryan (2009)
Measuring single-cell density
William H. Grover (2011)
Optical volume and mass measurements show that mammalian cells swell during mitosis
E. Zlotek-Zlotkiewicz (2015)
Biophysical changes reduce energetic demand in growth factor–deprived lymphocytes
Vivian C. Hecht (2016)
Label-free DNA imaging in vivo with stimulated Raman scattering microscopy
Fa-Ke Lu (2015)
Intracellular Water Exchange for Measuring the Dry Mass, Water Mass and Changes in Chemical Composition of Living Cells
Francisco Feijó Delgado (2013)
Modulation of Chemical Composition and Other Parameters of the Cell at Different Exponential Growth Rates.
H. Bremer (2008)
A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: Implications for spore dormancy
A. Cowan (2003)
Osmostress-Induced Cell Volume Loss Delays Yeast Hog1 Signaling by Limiting Diffusion Processes and by Hog1-Specific Effects
Roja Babazadeh (2013)
Autoproteolytic activation of pro-caspases by oligomerization.
X. Yang (1998)
Fluorescence correlation spectroscopy: past, present, future.
E. Elson (2011)
Hyperspectral imaging with stimulated Raman scattering by chirped femtosecond lasers.
Dan Fu (2013)
Regulation of Transmembrane Signaling by Phase Separation.
Lindsay B Case (2019)
Genomic expression programs in the response of yeast cells to environmental changes.
A. Gasch (2000)
Oligomerization Is a General Mechanism for the Activation of Apoptosis Initiator and Inflammatory Procaspases*
D. Chang (2003)
Density-gradient centrifugation enables the purification of cultured corneal endothelial cells for cell therapy by eliminating senescent cells
N. Okumura (2015)
Decoupling of Rates of Protein Synthesis from Cell Expansion Leads to Supergrowth.
Benjamin Knapp (2019)
The osmotic insensitivity of sonicated liposomes and the density of phospholipid-cholesterol mixtures.
S. M. Johnson (1973)
Cytoplasmic condensation is both necessary and sufficient to induce apoptotic cell death
Nola Jean Ernest (2008)
Macromolecular Crowding In Vitro, In Vivo, and In Between.
G. Rivas (2016)
Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts
M. Lohka (1985)
Inflating bacterial cells by increased protein synthesis
Markus Basan (2015)
Synergy between the small intrinsically disordered protein Hsp12 and trehalose sustain viability after severe desiccation
Skylar Xantus Kim (2018)
Living with water stress: evolution of osmolyte systems.
P. Yancey (1982)
Coordination of growth with cell division in the yeast Saccharomyces cerevisiae.
G. C. Johnston (1977)
Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
C. Freudiger (2008)
Increasing intracellular trehalose is sufficient to confer desiccation tolerance to Saccharomyces cerevisiae
Hugo Tapia (2015)
Severe osmotic compression triggers a slowdown of intracellular signaling, which can be explained by molecular crowding
Agnès Miermont (2013)
Down-regulation of TORC2-Ypk1 signaling promotes MAPK-independent survival under hyperosmotic stress
A. Muir (2015)
Decrease in Plasma Membrane Tension Triggers PtdIns(4,5)P2 Phase Separation to Inactivate TORC2
Margot Riggi (2018)
Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation.
Thomas C. Boothby (2017)
An integrated view on a eukaryotic osmoregulation system
S. Hohmann (2015)
Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures.
L. Hartwell (1970)
Disorders of erythrocyte hydration.
Patrick G. Gallagher (2017)
mTOR Signaling in Growth, Metabolism, and Disease
Robert A Saxton (2017)
Quantitative 3D imaging of whole, unstained cells by using X-ray diffraction microscopy
H. Jiang (2010)
Imaging Live Cells Using Quantum Dots.
Jyoti K. Jaiswal (2015)
Average protein density is a molecular‐weight‐dependent function
H. Fischer (2004)
Adipose tissue density, estimated adipose lipid fraction and whole body adiposity in male cadavers.
A. D. Martin (1994)
Measuring Cellular Biomass Composition for Computational Biology Applications
Ashley E. Beck (2018)
In situ measurement of absolute concentrations by Normalized Raman Imaging
Seungeun Oh (2019)
Dilution and titration of cell-cycle regulators may control cell size in budding yeast
F. S. Heldt (2018)
A practicable and accurate method to differentiate between intra- and extracellular water of microbial cells.
P. Illmer (1999)
Hormone-induced cell death. Purification ad properties of thymocytes undergoing apoptosis after glucocorticoid treatment.
A. Wyllie (1982)
Cyclin D-Cdk4,6 Drives Cell-Cycle Progression via the Retinoblastoma Protein's C-Terminal Helix.
Benjamin R. Topacio (2019)
Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate
E. Sokolova. (2013)
mTORC1 Controls Phase Separation and the Biophysical Properties of the Cytoplasm by Tuning Crowding
M. Delarue (2018)
Tubulin dynamics in cultured mammalian cells
W. Saxton (1984)
Intracellular Macromolecules in Cell Volume Control and Methods of Their Quantification.
M. Model (2018)
Isolation of Yeast Ascospores Free of Vegetative Cell Contamination
A. Chatterjee (1982)
The Chemical Components of a Cell
B. Alberts (2002)
A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy
M. Munder (2016)
Sterilization of Microorganisms with Supercritical Carbon Dioxide
M. Kamihira (1987)
Phase separation of 53BP1 determines liquid‐like behavior of DNA repair compartments
S. Kilic (2019)
Weighing of biomolecules, single cells and single nanoparticles in fluid
T. Burg (2007)
Use of quantum dots for live cell imaging
Jyoti K. Jaiswal (2004)
Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis.
S. O'Rourke (2004)
DNA Content, Cell Size, and Cell Senescence.
R. Veitia (2019)
Cellular concentrations of enzymes and their substrates.
K. R. Albe (1990)
Nucleoid Size Scaling and Intracellular Organization of Translation across Bacteria
W. T. Gray (2019)
Bacterial dry matter content and biomass estimations.
G. Bratbak (1984)
Nutrient sensing and TOR signaling in yeast and mammals
A. González (2017)
Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway
P. Novick (1980)
Hog1 Mitogen-Activated Protein Kinase Phosphorylation Targets the Yeast Fps1 Aquaglyceroporin for Endocytosis, Thereby Rendering Cells Resistant to Acetic Acid
M. Mollapour (2007)
Molecular sieving properties of the cytoplasm of Escherichia coli and consequences of osmotic stress
Jacek T. Mika (2010)
Universal behavior of the osmotically compressed cell and its analogy to the colloidal glass transition
E. Zhou (2009)
Combining Three-Dimensional Quantitative Phase Imaging and Fluorescence Microscopy for the Study of Cell Pathophysiology
Y. Kim (2018)
Plasma membrane stress induces relocalization of Slm proteins and activation of TORC2 to promote sphingolipid synthesis
Doris Berchtold (2012)
Subdiffusion of loci and cytoplasmic particles are different in compressed Escherichia coli cells
S. Yu (2018)
Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains
Ann Boija (2018)
A glucose-starvation response regulates the diffusion of macromolecules
Ryan Joyner (2016)
Quantitative phase imaging in biomedicine
Y. Park (2018)
Effect of Macromolecular Crowding Agents on Human Immunodeficiency Virus Type 1 Capsid Protein Assembly In Vitro
M. del Álamo (2005)
Influence of oxygen on the growth of Saccharomyces cerevisiae in continuous culture
K. Furukawa (1983)
Bacterial spore heat resistance correlated with water content, wet density, and protoplast/sporoplast volume ratio.
T. C. Beaman (1982)
Dynamic instability of microtubule growth
T. Mitchison (1984)
Enzymatic replication of the origin of the Escherichia coli chromosome.
Robert S Fuller (1981)
This paper is referenced by
Biologically-informed neural networks guide mechanistic modeling from sparse experimental data
John H. Lagergren (2020)
Computationally enhanced quantitative phase microscopy reveals autonomous oscillations in mammalian cell growth
Xili Liu (2020)
The environmental stress response causes ribosome loss in aneuploid yeast cells
Allegra Terhorst (2020)
The environmental stress response causes ribosome loss in aneuploid yeast cells
Allegra Terhorst (2020)
Physical properties of the cytoplasm modulate the rates of microtubule growth and shrinkage
A. T. Molines (2020)
Variations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast
Pascal D. Odermatt (2020)